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1	Vliv promotorové sekvence na využití NAD+ jako substrátu pro iniciaci transkripce RNA polymerázou / Effect of promoter sequence on utilization of NAD+ as a substrate for transcription initiation by RNA polymerase Pinkas, Daniel January 2018 (has links) For a long time, 5' cap has been thought to be privilege only for eukaryotic organisms in form of 7-methylguanosine cap at the end of mRNA. This was changed only a few years ago. By using methods liquid chromatography and mass spectrometry a new molecule associated with RNA of Escherichia coli has been found. This molecule turned out to be nicotinamide adenine dinucleotide (NAD+ ) attached to 5' end of some small regulatory RNAs (sRNA). Later it has been shown, that RNA polymerase can attach NAD+ at 5' of RNA ab initio, meaning that RNA polymerase can utilize NAD+ as a substrate for transcription initiation. To some extent substrate for transcription initiation is chosen based on promoter sequence. Crucial requirement is presence of adenine at +1 position of DNA coding strand. This thesis focuses on promoter sequence requirements for transcription initiation with NAD+ . As a template for transcription four promoters with different modifications and their chimeras are used: RNA1, Pveg, lac UV5 and rrnB P1. Also, I tried to compare RNA polymerase from E. coli and B. subtilis in terms of transcription initiation substrate usage. Lastly, I describe here isolation of NudC, enzyme that cleaves NAD+ to nicotinamide mononucleotide (NMN) and adenosine monophosphate (AMP). NudC will be used for upcoming...
2	Analysis of Neuronal Diseases in the Model Organism <i>Aspergillus nidulans</i> / Die Analyse neuronaler Krankheiten im Modellorganismus <i>Aspergillus nidulans</i> Laubinger, Karen 29 October 2008 (has links) No description available. 570 Biowissenschaften, Biologie Mathematics and Computer Science Aspergillus nidulans α-Synuklein NUDC NUDF Parkinson Lissenzephalie Aspergillus nidulans α-synuclein NUDC NUDF Parkinson 42.13 42.15 42.20 42.30 42.51 WF 200: Molekularbiologie WJD 300: Mutationen {Biologie, Genetik} WUV 000: Angewandte Mikrobiologie

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Vliv promotorové sekvence na využití NAD+ jako substrátu pro iniciaci transkripce RNA polymerázou / Effect of promoter sequence on utilization of NAD+ as a substrate for transcription initiation by RNA polymerase

Analysis of Neuronal Diseases in the Model Organism <i>Aspergillus nidulans</i> / Die Analyse neuronaler Krankheiten im Modellorganismus <i>Aspergillus nidulans</i>