Made available in DSpace on 2017-07-10T17:37:30Z (GMT). No. of bitstreams: 1 Vanessa Aparecida Antes.pdf: 571702 bytes, checksum: c629643a9f77bdb02f4dd1641cbecf68 (MD5) Previous issue date: 2008-08-29 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The aim of this work was to study the parasitism of Meloidogyne incognita on native plants from Western Paraná, Brazil, as well as to assess the genetic variability among different populations belonging to the race 3 of this nematode by RAPD technique. Natural infection was studied in thirty six native plant species, which were identified on the basis of the perineal pattern from mature females and esterase phenotype. Native species that showed no infestation on the root system were inoculated with 1.000 eggs and/or J2 of M. incognita. After 60 days, the inoculated plants were evaluated regarding the number of galls and the number of eggs/or J2 per root. The genetic variability from different single female populations of M. incognita race 3 was studied by RAPD technique, having been tested 10 primers. The native plants that were susceptible to M. incognita parasitism were Rabo-de-Bugio (Lonchocarpus muehlbergianus Hassl.), Ipê Roxo (Tabebuia impetiginosa Mart. Ex DC. Standl), Sanga D água (Croton urucurana Boill), Ipê Amarelo (Tabebuia ahrysotricha Mart ex DC. Standl.), Genipapo (Genipa americana L.), Ariticum Comum (Rollinia mucosa (Jacq.) Baill.) and Aroeira Vermelha (Schinus terebinthifolius Raddi.). On the other hand, M. javanica was found parasiting Café de Bugre (Cordia ecalyculata Vell.), Guatambu Vermelho (Aspidosperma subincanun Mart.) and Tarumã Branco (Cytrarexllum myruanthum Cham.). The DNA polymorphism showed that there was genetic variability among populations from a same race (3) of M. incognita, allowing the separation of them into five genetic groups, through reactions with the primers (O)AK20, A10, AQ12, AS08 and (OP)F01 / Objetivou-se com o presente trabalho estudar o parasitismo de Meloidogyne incognita em plantas nativas do oeste paranaense, bem como a variabilidade genética de populações de M. incognita raça 3 pela técnica RAPD. Trinta e seis espécies de plantas nativas foram analisadas com relação à infecção natural por Meloidogyne spp. A identificação de Meloidogyne foi feita com base na configuração perineal de fêmeas maduras e no fenótipo para a isoenzima esterase. Espécies nativas com ausência de galhas no sistema radicular foram inoculadas com 1.000 ovos e/ou J2 de uma população de campo de M. incognita. Após 60 dias da inoculação, as plantas foram avaliadas com relação ao número de galhas e número de ovos e/ou juvenis por raiz. A variabilidade genética de diferentes populações monoespecíficas de M. incognita raça 3 do noroeste paranaense foi estudada pela técnica RAPD com o teste de 10 primers. As plantas nativas hospedeiras de M. incognita foram Rabo-de-Bugio (Lonchocarpus muehlbergianus Hassl.), Ipê Roxo (Tabebuia impetiginosa Mart. Ex DC. Standl), Sanga D água (Croton urucurana Boill), Ipê Amarelo (Tabebuia ahrysotricha Mart ex DC. Standl.), Genipapo (Genipa americana L.), Ariticum Comum (Rollinia mucosa (Jacq.) Baill.) e Aroeira Vermelha (Schinus terebinthifolius Raddi.). Já M. javanica foi encontrado parasitando Café de Bugre (Cordia ecalyculata Vell.), Guatambu Vermelho (Aspidosperma subincanun Mart.) e Tarumã Branco (Cytrarexllum myruanthum Cham.). A análise do polimorfismo do DNA possibilitou a detecção de variabilidade genética para diferentes populações de uma mesma raça (3) de M. incognita, permitindo a separação das populações em 5 grupos genéticos, através de reações com os primers AK20, A10, AQ12, AS08 e F01

The Bureau of Land Managements (BLM) Emergency Fire Rehabilitation (EFR) policy was developed in 1985 to encourage protection of sites from soil erosion and to minimize potential changes in vegetation communities that may result from the dominance of weedy species. To achieve the goals of EFR policy, managers often used introduced perennial grasses that established quicker and competed better with introduced annuals than did native plants. However, the change of sagebrush-grass communities to communities dominated by introduced forage grasses has led to concerns for wildlife habitat. This concern contributed to a policy change encouraging the use of native species, when available, for rehabilitation projects. This study attempts to assess the effectiveness of BLM EFR projects in meeting the stated goals of the BLM EFR policy in the Great Basin. To do this, two field offices per state were randomly selected from an inclusive list of all Great Basin field offices. In 2001, we randomly selected three EFR projects per field office from those projects that used native species. On each project site, we used a common monitoring technique in association with monitoring techniques implemented by the BLM to assess if national EFR objectives were being met. A semi-structured survey was developed to determine the potential reasons why native and introduced plants were either used or not used, why monitoring was and was not proposed, and whether monitoring was implemented in rehabilitation projects. BLM monitoring techniques did not adequately evaluate EFR goal achievement. The time it took to implement any of the BLM methods did not differ significantly from the time needed to implement the common protocol on the two projects where BLM had implemented monitoring and used native plants (F[subscript 3,12]=1.63, P=0.23). Cost to implement the common monitoring technique was minimal and it directly measured aspects of stated EFR policy goals. Vegetative cover of all natives, seeded and volunteers, contributed half of the overall cover on EFR projects and was significantly higher than sown introduced species. Invasive species were intermediate and did not differ significantly from either the natives or the introduced. The seeded species were a subset of the native or introduced classes. Composition by cover between sown native, sown introduced, and invasive species did not differ significantly. Vegetation cover increased the surface soil stability 39% of the time and subsurface stability 56% beneath the vegetation. Respondents of the survey stated that they generally use more natives and more complex seed mixtures than they did historically. Many also stated that they prefer to use native over introduced species. However, most felt that introduced species are more effective in meeting EFR goals on the degraded sites than native species. All respondents would like to access a summarized report of other rehabilitation projects. The respondents were split between accessing it through the World Wide Web or through a written report. We believe that a common database could be created and maintained on the World Wide Web if a common sampling protocol was implemented. / Graduation date: 2004

The contamination of soil for copper had refusal contribute to plants establishment in mining and vineyards areas of South Brazil. Mycorrhyzal forestry species could be one alternative to viable the development of plants in contaminated areas for heavy metals. The work was developed at Soil Department and Botanical Garden, in Federal University of Santa Maria; FEPAGRO- FLORESATA, Santa Maria-RS and Camaquã Mines, RS. Firstly, it was evaluated the mycorrhizal occurrence in forestry species like Luehea divricata Mart. et Zucc., Parapiptadenia rigida (Benth) Brenan, Peltophorum dubium (Sprengel) Tauber., Cedrela fissilis Vell., Eugenia involucrata Dc., Fícus puschnathiana (Miq.) Miq., Apuleia Ieiocarpa (J. Vogel) J.F. Macbr., Inga marginata Willd., Tabebuia Alba (Cham.) Sandwith, Tabebuia heptaphylla (Vell.) Toledo, Enterolobium contortisiliquum (Vell.) Morong., Tipuana tipu (Benth.) O. Kuntze and Phytolacca dioica L. Roots and soil sampling were performed at Botanical Garden of Federal University of Santa Maria and in native forest on Boca do Monte, district of Santa Maria, RS. No one of studied species had found ectomycorrhizal association in natural environment conditions. Roots of Luehea divricata, Parapiptadenia rigida, Peltophorum dubium, Cedrela fissilis, Fícus puschnathiana, Ingá marginata, Tabebuia Alba, Tabebuia heptaphylla, Enterolobium contortisiliquum, Tipuana tipu and Phytolacca dioica had arbuscular mycorrhizal. An experiment in liquid and solid culture medium was conducted to select copper tolerant ectomycorrhizal fungi. The fungi tested in solid medium were: Pisolitus microcarpus UFSC-Pt116, Suillus sp. UFSM RA2.8, Suillus sp. UFSM RA118, Scleroderma sp. UFSM SC3.6 and Scleroderma sp. UFSC-Sc124. In liquid culture medium were tested the species: Pisolitus microcarpus UFSC-Pt116, Pisolitus tinctorius UFSC-Pt24, Scleroderma sp. UFSC-Sc124, UFSM MC and UFSM RA2.8. The fungi UFSC-Pt116 showed copper tolerance and produced high amount of fungi mycelium. Latter, it was characterized ectomycorrizal on Parapiptadenia rigida, Peltophorum dubium, Apuleia Ieiocarpa and Enterolobium contortisiliquum seedlings in vitro conditions, cultivated and previously inoculated with the ectomycorrhizal fungi UFSC-Pt116. Seedlings of Parapiptadenia rigida, Peltophorum dubium and Enterolobium contortisiliquum was formed association with UFSC Pt 116. The region of transcribed internal space was studied and evaluated the differences in sequencing polymorphism of this region of isolated UFSC-Pt116, it was compared to other ectomycorrhizal fungi like Pisolithus, Sclerodrema, Rhizopogon and Suillus available in the GenBank data. It was confirmed the morphological identification of the isolates as belonging to the genus Pisolithus. However, with molecular results did not confirm the species of this fungus. The second part, it was evaluated the tolerance to copper for forestry species like Parapiptadenia rigida, Peltophorum dubium, Cedrela fissilis and Enterolobium contortisiliquum. Seedlings of Cedrela fissilis was evidenced copper accumulation on leaves, stem and roots. Seedlings of Enterolobium contortisiliquum was accumulated copper on roots, but the metal was not translocated to stem and leaves. It is possible to consider Parapiptadenia rigida as a cooper excluding plant. Peltophorum dubium, Parapiptadenia rigida and Enterolobium contortisiliquum was showed high tolerance to contaminated soil with copper. The thirdly part, it was evaluated the behavior Peltophorum dubium seedlings inoculated with the UFSC Pt 116 ectomycorrhizal fungi in soil with high copper concentrations. The increasing of copper doses was reduced root length, specific root surface area, height and stem diameter of Peltophorum plants. Seedlings of inoculated Peltophorum were showed less copper concentration on tissues and reduced copper absorption on more elevated doses. It was concluded that isolates UFSC-Pt116, UFSC-Pt24 and UFSC-SC124 were efficient on mycelium production in medium with high copper amounts. The isolated UFSC-Pt116 was not efficient to promote Peltophorum growth in soil with high copper concentration, but this isolated was reduced the copper absorption for plant. / A contaminação do solo por cobre tem contribuído negativamente para o estabelecimento de plantas em áreas de mineração e áreas vinícolas do sul do Brasil. Espécies florestais micorrizadas podem ser uma alternativa para viabilizar o desenvolvimento de plantas em áreas contaminadas por metais. O trabalho foi desenvolvido no Departamento de Solos, no Jardim Botânico da Universidade Federal de Santa Maria; na FEPAGRO FLORESTA, Santa Maria e nas Minas do Camaquã, Caçapava do Sul, RS. A primeira etapa objetivou avaliar a ocorrência de associação micorrízica nas espécies florestais Luehea divricata Mart. et Zucc. (açoita-cavalo), Parapiptadenia rigida (Benth) Brenan (angico-vermelho), Peltophorum dubium (Sprengel) Tauber. (canafístula), Cedrela fissilis Vell. (cedro), Eugenia involucrata Dc. (cerejeira), Fícus puschnathiana (Miq.) Miq. (figueira-do-mato), Apuleia Ieiocarpa (J. Vogel) J.F. Macbr. (grápia), Inga marginata Willd. (ingá-feijão), Tabebuia Alba (Cham.) Sandwith (ipê-ouro), Tabebuia heptaphylla (Vell.) Toledo (ipê-roxo), Enterolobium contortisiliquum (Vell.) Morong. (timbaúva), Tipuana tipu (Benth.) O. Kuntze (tipuana) e Phytolacca dioica L. (umbu). As coletas de raízes e de solo foram realizadas no Jardim Botânico da Universidade Federal de Santa Maria e em mata nativa no distrito municipal de Boca do Monte, Santa Maria, RS. Nenhuma das espécies estudadas apresentaram associação com fungos ectomicorrízicos em ambiente natural. Raízes de açoita-cavalo, angico-vermelho, canafístula, cedro, figueira, ingá-feijão, ipê-ouro, ipê roxo, timbaúva, tipuana e umbu apresentam micorrizas arbusculares. Um segundo experimento foi desenvolvido para selecionar fungos ectomicorrízicos tolerante a cobre em meio de cultura líquido e sólido. Os fungos testados no meio de cultura sólido foram: Pisolithus microcarpus UFSC-Pt116, Suillus sp. UFSM RA2.8, Suillus sp. UFSM RA118, Scleroderma sp. UFSM SC3.6 e Scleroderma sp. UFSC-Sc124. No meio de cultura líquido foram testados os fungos: Pisolithus microcarpus UFSC-Pt116, Pisolithus tinctorius UFSC-Pt24, Scleroderma sp. UFSC Sc124, o fungo UFSM MC e o UFSM RA2.8. O fungo UFSC-Pt116 apresentou tolerância ao cobre e produziu maior quantidade de massa seca do micélio fúngico. Posteriormente, caracterizou-se a formação de ectomicorrizas em plântulas de angicovermelho, canafístula, grápia e timbaúva cultivadas in vitro, inoculadas com o fungo UFSCPt116. Plântulas de angico-vermelho, canafístula e timbaúva formam associação com fungo ectomicorrízico UFSC-Pt116. Estudou-se a região do espaço interno transcrito e analisaramse as diferenças no polimorfismo da seqüência desta região no isolado UFSC-Pt116 com as seqüências dos isolados de Pisolithus, Scleroderma, Rhizopogon e Suillus do GenBank. Confirmou-se a identificação morfológica do isolado como pertencente ao gênero Pisolithus. Contudo, com os resultados moleculares não foi possível confirmar a espécie do fungo. A segunda etapa objetivou avaliar o comportamento de angico-vermelho, canafístula, cedro e timbaúva tolerantes ao cobre. Mudas de cedro acumulam cobre na parte aérea e radicular. Mudas de timbaúva acumulam cobre na raiz da planta, não o translocam para a parte aérea. O angico-vermelho pode ser considerado uma planta exclusora de cobre. Canafístula, angicovermelho e timbaúva apresentaram maior tolerância a solo contaminado por cobre. A terceira etapa objetivou avaliar o comportamento de mudas de canafístula inoculadas com fungo ectomicorrízico em solo com excesso de cobre. O fungo ectomicorrízico utilizado como inoculante foi o isolado UFSC-Pt116. O incremento das doses de cobre reduziu o comprimento radicular, área superficial específica radicular, altura e diâmetro das plantas de canafístula. Mudas de canafístula inoculadas com fungo ectomicorrízico apresentaram menor teor de cobre em seu tecido e reduziram a absorção de cobre em dose mais elevada. Concluiuse que os isolados UFSC-Pt116, UFSC-Pt24 e UFSC-Sc124 foram eficientes in vitro na produção de micélio com elevada quantidade de cobre. O isolado UFSC-Pt116 não respondeu suficientemente para favorecer o desenvolvimento de canafístula em solo com excesso de cobre. Entretanto, este isolado reduziu a absorção de cobre pela planta.

Submitted by Claudia Rocha (claudia.rocha@udesc.br) on 2018-02-27T14:58:33Z No. of bitstreams: 1 PGPV16MA209.pdf: 5712412 bytes, checksum: 8b2731215560719c3583c42e4b347214 (MD5) / Made available in DSpace on 2018-02-27T14:58:33Z (GMT). No. of bitstreams: 1 PGPV16MA209.pdf: 5712412 bytes, checksum: 8b2731215560719c3583c42e4b347214 (MD5) Previous issue date: 2016-07-29 / Capes / Brazil is still considered a country with mega biodiversity, which corresponds to 44.830000 plant species, and some plants have ornamental potential. Many of these ornamental species are not used, and not even known by the population, which can help to increase the introduction of exotic species. Knowledge of native ornamental flora is a key factor for their conservation and sustainable management of the species. The objectives of this study were to know the native ornamental flora in field areas and forest edge through floristic survey and carry out phenological monitoring of four species. The study was conducted in the Mixed Ombrophilous Forest High-Montana, in a Private Reserve of the State Natural Heritage (RPPNE - Reserva Particular do Patrimônio Natural Estadual) Nascentes Farm, Urupema, SC. The property has a total area of 1367.43 hectares, located between the coordinates 27 52'37,33 "( South latitude) and 49 55 '14.39" (West longitude), with an altitude ranging from 1450 to 1750 meters and belongs to Klabin SA company. The weather is mild , humid, with well distributed rains throughout the period of the year and average annual rainfall of 1800 mm, the average annual temperature is 13 °C. The vegetation sampling was carried out from April/2015 to April/2016 in an area of approximately 32 hectares, adopting the traversal method, looking for specimens with ornamental potential. For phenological monitoring four species were selected: Callianthe fluviatilis (Vell.) Donnell (Malvaceae), Senecio icoglossus var. icoglossus, (Asteraceae), Sisyrinchium micranthum Cav. (Iridaceae) and Trichocline catharinensis Cabrera. The vegetative stages (sprouting and leaf fall) and reproductive (button, anthesis, unripe fruit and ripe fruit) of the species were biweekly accompanied from April 2015 to April 2016, using the qualitative method to evaluate each phenophase. They were made Spearman correlation analysis (rs) of phenophases with meteorological data, rainfall (mm), temperature (°C) and heat stroke (h), provided by EPAGRI /CIRAM. The floristic survey resulted in the registration of 23 families, 39 genera and 54 species with ornamental potential. The best represented families were Asteraceae (13 species), Iridaceae (5) and Lamiaceae (4) and for the other families, were recorded between the three species, Lythraceae, Solanaceae, and Verbenaceae (3) Apiaceae, Fabaceae, Melastomataceae, Onagraceae , Polygalaceae and Rubiaceae (2), Alstroemeriaceae Apocynaceae, Begoniaceae, Campanulaceae, Commelinaceae, Convolvulaceae, Eriocaulaceae, Lentibulariaceae, Liliaceae, Malvaceae and Orobanchaceae (1). The accompanied species presented flowering, fruiting, leaf fall and sprout in the studied months. The correlation analyzes showed that Callianthe fluviatilis species correlated with ripe fruit and insolation (r = 0.64; p = 0.0027), anthesis and minimum temperature (r = 0.058; p = 0.0092), button and minimum temperature (r = 0.62; p = 0.0041), immature fruit and maximum temperature (r = 0.75, p = 0.00016), ripe fruit and maximum temperature (r = 0.73; p = 0, 00032) ripe fruit and minimum temperature (r = 0.75, p = 0.00021). The species Senecio icoglossus DC. var. icoglossus showed positive correlation between ripe fruit and precipitation (r = 0.81; p = 0.0042), budding was negatively correlated with the minimum temperature (r = -0.84; p = 0.0018), leaf fall it was negatively correlated with minimum temperature (r = -0.84; p = 0.0018), leaf fall was negatively correlated with temperature and maximum (r = -0.80; p = 0.0052). Sisyrinchuim micranthum obtained positive correlation, ripe fruit and maximum temperature (r = 0.77; p = 0.0010), ripe fruit and minimum temperature (r = 0.82, p = 0.00029). Trichocline catharinensis showed highly significant correlation between buttons and minimum temperature (r = 0.69; p = 0.0003), and button and insolation (rs = 0.60; p = 0.0029). The four species studied were correlated with weather factors, environmental variables were important to the phenology of species with ornamental potential. / O Brasil é considerado um país com megabiodiversidade, o que corresponde a 44,830 mil espécies vegetais, e parte das plantas tem potencial ornamental. Muitas dessas espécies ornamentais não são utilizadas, e nem mesmo conhecidas pela população, que pode auxiliar ao aumento da introdução de espécies exóticas. O conhecimento da flora ornamental nativa é fator fundamental para sua conservação e manejo sustentável das espécies. Os objetivos deste estudo foram conhecer a flora ornamental nativas em áreas de campo e borda florestal, por meio de levantamento florístico, e realizar acompanhamento fenológico de quatro espécies. O estudo foi conduzido na Floresta Ombrófila Mista Alto-Montana, em uma Reserva Particular do Patrimônio Natural Estadual (RPPNE) Fazenda das Nascentes, Urupema, SC. A propriedade possui área total de 1.367,43 hectares, localizada entre as coordenadas 27º 52’37,33” (latitude) sul e 49º 55’ 14,39” (longitude) oeste, com altitude variando entre 1450 a 1750 metros e pertence à empresa Klabin SA. O clima da região é temperado, úmido, com chuvas bem distribuídas durante todo o período do ano e precipitação média anual de 1800 mm, a temperatura média anual é de 13 °C. A amostragem da vegetação foi realizada no período de 04/2015 a 04/2016 numa área de aproximadamente 32 hectares, adotando-se o método de caminhamento, em busca de espécimes com potencial ornamental. Para o acompanhamento fenológico foram selecionadas quatro espécies: Callianthe fluviatilis (Vell.) Donnell (Malvaceae), Senecio icoglossus var. icoglossus, (Asteraceae), Sisyrinchium micranthum Cav. (Iridaceae) e Trichocline catharinensis Cabrera. Foram acompanhadas quinzenalmente as fases vegetativa (brotação e queda foliar) e reprodutivas (botão, antese, frutos imaturos, e frutos maduros) das espécies no período de abril de 2015 a abril 2016, utilizando o método qualitativo para avaliar cada fenofase. Foram feitas a análise de correlação de Spearman (rs) das fenofases com os dados meteorológicos, precipitação (mm), temperatura (°C) e insolação (h), fornecidos pela EPAGRI/CIRAM. O levantamento florístico resultou no registro de 23 famílias, 39 gêneros e 54 espécies, com potencial ornamental. As famílias melhor representadas foram Asteraceae (13 espécies), Iridaceae (5) e Lamiaceae (4) e, para as demais famílias, foram registradas entre uma a três espécies, Lythraceae, Solanaceae e Verbenaceae (3) Apiaceae, Fabaceae, Melastomataceae, Onagraceae, Polygalaceae e Rubiaceae (2), Alstroemeriaceae Apocynaceae, Begoniaceae, Campanulaceae, Commelinaceae, Convolvulaceae, Eriocaulaceae, Lentibulariaceae, Liliaceae, Malvaceae e Orobanchaceae (1). As espécies acompanhadas apresentaram floração, frutificação, queda foliar e brotação nos meses estudados. As análises de correlação mostraram que a espécie Callianthe fluviatilis apresentou correlação com fruto maduro e insolação (r = 0,64; p = 0,0027), antese e temperatura mínima (r = 0,058; p = 0,0092), botão e temperatura mínima (r = 0,62; p = 0,0041), fruto imaturo e temperatura máxima (r = 0,75; p = 0,00016), fruto maduro e temperatura máxima (r = 0,73; p = 0,00032) fruto maduro e temperatura mínima (r = 0,75; p = 0,00021). A espécie Senecio icoglossus DC. var. icoglossus mostrou correlação positiva entre fruto maduro e precipitação (r = 0,81; p = 0,0042), a brotação foi correlacionada negativamente com a temperatura mínima (r = -0,84; p = 0,0018), a queda foliar foi correlacionada negativamente com temperatura mínima (r = -0,84; p = 0,0018), a queda foliar foi correlacionada negativamente com e temperatura máxima (r = -0,80; p = 0,0052). Sisyrinchuim micranthum obteve correlação positiva, fruto maduro e temperatura máxima (r = 0,77; p = 0,0010), fruto maduro e temperatura mínima (r = 0,82; p = 0,00029). Trichocline catharinensis apresentou correlação altamente significativa entre botões e temperatura mínima (rs = 0,69; p = 0,0003) e, botão e insolação (rs = 0,60; p = 0,0029). As quatro espécies estudadas tiveram correlação com os fatores meteorológicos, as variáveis ambientais foram importantes para os eventos fenológicos das espécies com potencial ornamental.

Durante muitas décadas, extensas áreas de florestas nativas têm sido sistematicamente destruídas no Brasil, muitas delas dando lugar a plantações florestais para fins industriais. A adequação à legislação ambiental recente, no que se refere à manutenção de áreas de preservação permanente e de reservas legais, passou a gerar a necessidade de reversão de áreas ocupadas com plantios comerciais para florestas nativas. Este trabalho teve como objetivo estudar a efetividade de técnicas para acelerar a regeneração natural da vegetação nativa em talhões de Eucalyptus saligna abandonados. O estudo foi realizado em áreas pertencentes à Suzano Papel e Celulose, onde foi criado o Parque das Neblinas, município de Bertioga, SP. A área experimental foi instalada em um talhão de terceira rotação com 45 ha, manejado anteriormente em ciclos de corte de sete anos. O experimento foi em blocos ao acaso com seis tratamentos e quatro repetições: T1=controle, T2-T5=redução de 25, 50, 75 e 100% da área basal, respectivamente, através de envenenamento com glyphosate; T6=corte raso. Cada parcela apresentou 20 m x 40 m. Inicialmente foi realizado um inventário da população de Eucalyptus saligna, bem como o levantamento fitossociológico de toda a regeneração natural existente acima de 1,30 m de altura. Foram mensurados 1.417 indivíduos de Eucalyptus saligna, com densidade absoluta de 738,02 ind/ha e área basal de 22,68 m²/ha. O método utilizado para morte em pé dos eucalitpos se mostrou eficiente. No conjunto dos 5.863 indivíduos amostrados da vegetação nativa, foram identificadas 135 espécies, pertencentes a 77 gêneros e a 39 famílias. A densidade total estimada foi de 1.035,41 indivíduos/ha e a área basal de 6,39 m²/ha, para a classe de indivíduos da regeneração natural com DAP 5 cm e de 3.864,58 indivíduos/ha e área basal de 3,25 m²/ ha, para regeneração natural com DAP < 5 cm e maior que 1,30 m de altura. Não houve efeito significativo dos tratamentos a 5% de probabilidade para as variáveis estruturais. Entretanto, o estudo da riqueza de espécies corrigida (correção feita usando-se o índice de Fisher) para a regeneração com DAP 5 cm revelou um decréscimo nas parcelas testemunha entre as fases inicial e final e um aumento deste índice para os demais tratamentos. Além disso, a variação percentual do índice de diversidade de Shannon (H) entre as épocas inicial e final apresentou diferenças significativas entre tratamentos e blocos, sendo que o T3 propiciou significativamente os maiores aumentos da diversidade de espécies (H). Embora não tenha havido efeito do tratamento na área basal da regeneração natural, houve uma correlação linear negativa desta variável com a área basal do eucalipto. O resultado indica uma tendência de aumento de área basal da vegetação nativa com DAP 5 cm à medida que se reduz a área basal do eucalipto. As análises mostraram que o T3 (redução de 50% da área basal do eucalipto através de morte em pé das árvores) propiciou os melhores resultados para densidade, área basal e riqueza de espécies para as duas classes de regeneração natural, após 40 meses. / For decades, large areas of native forests have been and still systematically destroyed in Brazil, many of them leading to forest plantations stands for industrial purposes. The adequacy to the recent environmental legislation regarding the maintenance of permanent preservation areas and legal reserves required reversing the areas occupied with commercial plantations into native forests. This study aimed at verifying the effect of techniques to accelerate the natural regeneration of native vegetation in abandoned production stands of Eucalyptus saligna. The study was conducted in areas belonging to the Suzano Papel e Celulose, at Parque das Neblinas, Bertioga, SP. The experiment was set up in a commercial Eucalyptus saligna stand with 45 ha, third rotation, previously managed in seven-year cutting cycles. The experimental design was in randomized blocks with six treatments and four replications. The treatments were: T1= control; T2-T5= reduction of 25, 50, 75 and 100% of basal area, respectively, through girdling and poisoning and T6= clearcut. Each plot had 20 x 40 meters. An inventory on the Eucalyptus saligna population was performed, as well as a phytosociological survey of the entire natural regeneration over 1.3 m in height 1,417 individuals of Eucalyptus saligna were measured and showed an absolute density of 738.02 ind/ha and 22.68 m²/ha basal area. As to the treatment applied (T2-T5) to the eucalyptus individuals, the method was proved efficient. We sampled 5,863 individuals of native vegetation belonging to 135 species of 77 genera and 39 families. The total density estimated was 1,035.41 individuals/ha and the basal area was 6.39 m²/ha for the DBH 5 cm class and 3,864.58 individuals/ha and basal area of 3.25 m²/ ha for the natural regeneration with DBH < 5 cm. There was no significant effect of the treatments at a 5% probability level for the variables studied. Although, the richness analysis corrected (correction through the Fishers index) for the DBH 5 cm class showed decrease in the control plots between the initial and final phases, and an increase in the richness of the remaining treatments. In addition, the percent variation of the Shannons diversity index between the initial and final times had a significant effect for treatments and blocks, being the T3 showed significant increase in species diversity (H). Although no significant effect was observed for the basal area, a negative linear correlation of this variable with the eucalypt basal area was observed. The result points to a trend of increase in the basal area of the native vegetation with DBH > 5 cm as the eucalypt basal area is reduced. T3 (50 % reduction of the eucalypt basal area by poisoning) showed best results for density, basal area and species richness of for both natural regeneration classes after 40 months.

Native and naturalized California plant species were screened for their phytoremediation potential for the cleanup of soil contaminated with petroleum hydrocarbons (PHCs), poly-aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), chlorinated dioxins/furans, and heavy metals. This screening was followed by controlled greenhouse experiments to further evaluate the phytoremediation potential of the best candidates. Field specimens and soils used for this study were collected from the former Energy Technology Engineering Center (ETEC) at the Santa Susana Field Laboratory in Southern California that was operated by the Department of Energy (DOE). Soils at this site contain all of the contaminants of interest (COIs). Nine plant species were screened in the field: Purple Needlegrass (Nassella pulchra), Blue Elderberry (Sambucus nigra), Laurel Sumac (Malosma laurina), Mule Fat (Baccharis salicifolia), Palmer’s Goldenbush (Ericameria palmeri), Summer Mustard (Hirschfeldia incana), Narrowleaf Milkweed (Asclepias fascicularis), Coyote Brush (Baccharis pilularis), and Thickleaf Yerba Santa (Eriodictyon crassifolium). In the field three samples of each species growing in contaminated soil and one of each species growing in uncontaminated soil were selected for harvesting and analysis. The roots, above ground plant tissue, and soil around the roots were sampled separately and analyzed for the COIs: PHCs, PAHs, PCBs, chlorinated dioxins/furans, and metals (which include mercury, silver, cadmium, and lead). All of the plants in the field appeared to generate compounds which result in higher measured PHC concentrations than those measured in the associated soil. The highest concentrations of PAHs in the roots were observed for Blue Elderberry (1740 ug/kg), Purple Needlegrass (703 ug/kg), and Yerba Santa (200 ug/kg). No uptake of PCBs was observed in the roots or foliage of any species. The highest concentrations of total chlorinated dioxins/furans in the roots were observed for Purple Needlegrass (2237 ng/kg), Blue Elderberry (1026 ng/kg), Palmer’s Goldenbush (432 ng/kg), and Yerba Santa (421 ng/kg). The highest concentrations of total chlorinated dioxins/furans in the foliage were observed for Yerba Santa (901 ng/kg), Palmer’s Goldenbush (757 ng/kg), and Purple Needlegrass (694 ng/kg). No uptake of mercury was observed in the roots or foliage of any species. The highest concentration of silver in the roots was observed for Laurel Sumac (7.34 mg/kg). Summer Mustard (SM) was the only species that showed uptake of silver into the foliage (0.405 mg/kg). The highest concentrations of cadmium in the roots and foliage were observed for Mule Fat (1.84 mg/kg and 3.64 mg/kg) and Coyote Brush (1.52 mg/kg and 2.12 mg/kg) and the greatest concentration of lead in the roots and foliage was observed for Purple Needlegrass (8.92 mg/kg and 1.17 mg/kg). Plants with a wide variety of observed contaminant uptake in the field were selected for a second phase of research in which three of the most promising species were grown in greenhouse microcosms to quantify the removal of contaminants from the soil. The three species selected based on preliminary results from the field study were Coyote Brush, Mule Fat, and Purple Needlegrass. Microcosms consisted of 2.17 kg of soil in 4-L glass jars with glass marbles for an underdrain. Plants were watered with deionized water and no leachate was collected. Five replicates of each microcosm type were created and incubated for 211 days with soil sampling at 85 and 211 days. Soil, plant roots/above ground tissue, and volatilization from the plants were analyzed for COIs to determine the mechanisms of phytoremediation. One set of microcosms was used to test the effect of addition of achelating agent (ethylenediaminetetraacetic acid) and another set was used to test the effect of fertilizer addition on phytoremediation potential. Three control treatments were tested: sterilized (gamma irradiation) soil planted with Purple Needlegrass, unplanted soil, and sterilized unplanted soil. None of the plant species demonstrated volatilization of COIs under these conditions. Volatilization of mercury was not tested for. The average PCB concentration (measured as Aroclor 1260) reductions in soils with Purple Needlegrass and chelated Coyote Brush were 49.4% and 51.4% respectively (p < 0.05). However, the sterilized unplanted control also had a decrease of Aroclor 1260 concentrations in the soil of 36.6% (p < 0.05). None of the species phytoextracted PCBs, so the mechanism of PCB remediation appears to be phytostimulation of the rhizosphere. Purple Needlegrass showed the greatest uptake of dioxins/furans into the foliage but did not appear to reduce the dioxin/furan concentrations in the soil. Coyote Brush, fertilized Coyote Brush, and Mule Fat also showed uptake of dioxins/furans into the roots and foliage. Only the Coyote Brush and fertilized Coyote Brush significantly (p = 0.036, p = 0.022) reduced the total dioxin/furan concentration in the soil (17.8% and 19.8% respectively). Coyote Brush may have stimulated microbes in the rhizosphere to better degrade the dioxins/furans. None of the plants were identified as hyper-accumulators of metals, and none of the soil metal concentrations significantly decreased in any of the microcosms. All of the metals (except mercury) were taken into the roots of plants to some degree, with Purple Needlegrass showing the most promise for metal extraction as it showed some of the highest concentrations of metals in roots and was the only species that contained mercury and silver in the foliage. This study suggests that there is some potential for phytoremediation of PCBs and chlorinated dioxins/furans. The results for petroleum hydrocarbons were inconclusive. Metal uptake was not substantial enough to lower metal concentrations in the soils. Thus phytoremediation of COIs at the site is limited and more aggressive forms of remediation may be required to reduce the concentrations of COIs quickly.

Invasive plants represent an important topic of study in current ecology because of their effects on whole ecosystems. The plants interact with the soil including soil biota, with the other plants in the community and with other organisms, eg. herbivores. Invasive plants often differ from non- invasive plants in nutrient utilization and can thus affect soil pH as well. They may also differ in the way they interact with mycorrhizal fungi that help the plants with nutrient uptake. In this study, the effect of invasive and native plants on soil properties is compared. Congeneric pairs of species, where one species is native and the other invasive, are compared. The native species are chosen so that they are dominant and therefore comparable to the invasive plants in the new environment. The evaluated soil properties are pH value and content of elemental nutrients from abiotic properties, and the amount of mycorrhizal fungi propagules and their spreading rate in soil from the biotic properties. In this study, the invasive and native plants differ only in the content of exchangeable phosphorus and potassium. Content of these two nutrients and one of the indicators of mycorrhizal fungi differ within the pairs of species as well. For most soil properties, the genus of the plant plays the main role, not...

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Many plants are not commercially produced due to a lack of accessible information on their horticultural requirements and landscape potential. Members of the Legume Family (Leguminosae) are often conspicuous components of the vegetation of arid and semi-arid subtropical regions. Many of these plants are suitable for landscaping use in areas suited to their cultivation. Coursetia glandulosa, Erythrina flabelliformis, Eysenhardtia orthocarpa, Haematoxylon brasiletto, Lysiloma watsonii, Pithecellobium mexicanum, and Sophora arizonica are woody legumes native to the Sonoran Desert region which offer a variety of form, texture, color and function. All of these plants grow readily from scarified seed. E. flabelliformis and E. orthocarpa are easy to propagate from stem cuttings. Some irrigation is necessary for establishment and reasonable growth in the landscape. Maintenance and pests are minimal. Freezing temperatures are the primary limiting factor to several of the plants. S. arizonica is slow growing and is prone to rot in the nursery.

Ações de recuperações de áreas degradadas têm sido amplamente empregadas, tanto por exigências legais como por iniciativas de grupos preocupados com a qualidade ambiental, assim não havendo uma padronização destas ações. Surgindo então a necessidade de avaliar se as metodologias empregadas estão alcançando seu objetivo e proporcionando uma auto-sustentação da comunidade florestal formada. O presente trabalho teve como objetivo descrever a comunidade arbustivo-arbórea de uma área restaurada, por meio de um plantio com alta diversidade, após 18 a 20 anos de sua implantação. O estudo foi desenvolvido em uma Floresta Estacional Semidecidual (FES) no município de Iracemápolis estado de São Paulo Sudeste do Brasil (22°35\'S e 47°31\'W). O clima regional é do tipo Cwa segundo a classificação de Köppen, com precipitação variando de 1.100 a 1.700mm por ano. O plantio foi realizado entre 1988 e 1990, em aproximadamente 50ha. Foi realizado um levantamento florístico e fitossociológico de duas áreas com 1,0ha cada, foram amostrados todos os indivíduos com perímetro a altura do peito igual ou maior que 10cm para calculo dos parâmetros fitossociológicos e dos índices de diversidade e de equidade. As espécies amostradas foram classificadas quanto a classe sucessional e a síndrome de dispersão. Foi verificada fisionomia florestal nas duas áreas estudadas. Foram encontrados 2.532 indivíduos vivos, distribuídos em 52,05% na área 1 e 47, 95% na área 2, e estavam distribuídos em 143 espécies, compondo 40 famílias. A altura do dossel foi inferior ao encontrado em remanescentes de FES, porém os índices de diversidade e equidade, bem como a densidade absoluta estiveram entre os valores obtidos para FES. As espécies que apresentaram maior crescimento foram Lafoensia glyptocarpa, Melia azedarach, Pterocarpus violaceus, Centrolobium tomentosum, Cariniana estrellensis, Triplaris americana, Cariniana legalis e Balfourodendron riedelianum na área 1 e Luehea divaricata, Pterogyne nitens, Citharexylum myrianthum e Triplaris americana na área 2. Foram verificados indivíduos com forma de vida vegetais diferentes dos introduzidos assim como espécies arbustivo-arbóreas diferentes das implantadas. A maior parte dos indivíduos regenerantes foi de espécies secundárias iniciais e pioneiras, sendo que as maiores espécies, tanto em altura como em área basal, foram provenientes das espécies plantadas. As duas áreas foram consideradas como restauradas e apresentam características de vegetação secundária. A composição inicial das espécies utilizadas e suas classes sucessionais influenciaram a composição da floresta formada com 18 e 20 anos. A utilização de alta diversidade em plantios de restauração apresenta-se de grande importância para o restabelecimento da estrutura, possibilitando o desenvolvimento dos processos existentes em florestas naturais nas áreas restauradas, especialmente em áreas não conectadas a fragmentos florestais naturais. / Restoration actions in degraded areas have been widely used, as much for legal requirements as for initiatives of groups worried about the ambient quality, in this way not having an standardizing of this actions. Appearing the necessity to evaluate if the employed methodologies are reaching its objective and providing a self-sustentation of the formed forest community. The present work had as objective to describe the community of tree and shrub from restored area, by a high diversity plantation, after 18 and 20 years of your implementation. The study was developed in Semideciduous Seasonal Forests (FES) at Iracemápolis City in São Paulo, Southeastern of Brazil (22°35\'S and 47°31\' W). The regional climate is Cwa, according to Köppen classification, with precipitation varying from 1.100 to 1.700mm per year. The plantation was realized between 1988 and 1990, in approximately 50ha. It was realized a floristic and phytosociological survey from two areas with 1,0ha each, where had been sampled individuals with perimeter at breast height equal or bigger than 10cm for calculate phytosociological parameter and diversity indices of equability. The showed species had been classified according to sucessional class and the syndrome of dispersion. Forest physiognomy was verified in the two studied areas. It has been found 2,532 individuals alive, distributed in 52,05% at area 1 and 47, 95% at area 2, and were distributed in 143 species, composing 40 families. The canopy heights had been less than found in FES remained, however, the diversity and equability index, and the absolute density has been among the value obtained to FES. The species that had presented greater growth had been Lafoensia glyptocarpa, Melia azedarach, Pterocarpus violaceus, Centrolobium tomentosum, Cariniana estrellensis, Triplaris americana, Cariniana legalis and Balfourodendron riedelianum in the area 1 and Luehea divaricata, Pterogyne nitens, Citharexylum myrianthum and Triplaris americana in area 2. Had been verified individuals with different vegetal life forms from introduced species as well as tree and shrub species different from implemented. Most of the regenerate individual was from pioneering and initial secondary species, and the biggest species, in height and in basal area had been coming from the planted species. It is concluded that the two areas are restored and present characteristic of secondary vegetation, the initial composition from used species and respective sucessional class had influenced the forest composition formed with 18 and 20 years. The use of high diversity in plantations of restoration present with high importance to re-establishing of the structure, enabling the development of existing process in a natural forests, especially in areas not connected with fragments of natural forests.