Dans cette étude, des aspects importants du rapport entre pesticides et abeilles domestiques (Apis mellifera L.) ont été traités. Dans la première partie, les effets de l’exposition des abeilles aux poussières contaminées avec trois neonicotinoides et le fipronil ont été étudiés. En fait, des quantités considérables de ces pesticides, utilisés pour l’enrobage des semences, sont dispersées pendant le semis du mais, et peuvent donc représenter une voie d’exposition des abeilles.En particulier, une voie spécifique d’exposition, le contact indirect, a été pris en compte. Les effets létaux et sub-létaux (mortalité aigue, butinage, développement des colonies, capacité d’orientation) des poussières ont été évalués en laboratoire, en tunnel et en plein champ. La dispersion réelle des poussières pendant le semis avait été évalué précédemment.Les résultats ont montré un effet significatif, sur la mortalité, de l’exposition aux poussières contaminées avec neonicotinoides et fipronil, en laboratoire et en tunnel. Par contre, la capacité d’orientation des abeilles n’a été pas influencée par l’exposition aux concentrations testées.Dans la deuxième partie de la recherche, on a étudié l’impact de différentes pesticides (chimiques et biologiques) sur les changements biochimiques et physiologiques des abeilles exposées. Ces paramètres on été évalué pour différentes façons et durées d’exposition. En particulier, trois expérimentations ont été effectuées en combinant les spores de Bacillus thuringiensis avec la deltamethine, les spores de Bt avec le fipronil et le traitement avec deltamethrine et le fongicide difenoconazole. Certains enzymes impliqués dans la détoxification, le stress oxydant et le métabolisme énergétique (GST, ALP, SOD, CAT, G6PDH, GAPDH) ont été sélectionnés pour évaluer les variations de leur activité suite à l’exposition aux pesticides. L’analyse des différents indicateurs biochimiques, comme le GST et le ALP, a mis en évidence des variations physiologiques qui peuvent être liés à l’exposition aux pesticides. Cette méthodologie pourrait donc représenter un nouvel aspect de l’évaluation des effets sub-létaux des pesticides chez l’abeille. / In this study, some important aspects of the relationship between honey bees (Apis mellifera L.) and pesticides have been investigated. In the first part of the research, the effects of the exposure of honey bees to neonicotinoids and fipronil contaminated dusts were analyzed. In fact, considerable amounts of these pesticides, employed for maize seed dressing treatments, may be dispersed during the sowing operations, thus representing a way of intoxication for honey bees. In particular, a specific way of exposure to this pesticides formulation, the indirect contact, was taken into account. To this aim, we conducted different experimentations, in laboratory, in semi-field and in open field conditions in order to assess the effects on mortality, foraging behaviour, colony development and capacity of orientation. The real dispersal of contaminated dusts was previously assessed in specific filed trials. The results showed a significant effect on mortality of neonicotinoids and fipronil contaminated dusts, both in laboratory and in semi-field trials. However, no effects were evidenced in honey bees orientation capacity.In the second part, the impact of various pesticides (chemical and biological) on honey bee biochemical-physiological changes, was evaluated. Different ways and durations of exposure to the tested products were also employed. Three experimentations were performed, combining Bt spores and deltamethrin, Bt spores and fipronil, difenoconazole and deltamethrin. Several important enzymes (GST, ALP, SOD, CAT, G6PDH, GAPDH) were selected in order to test the pesticides induced variations in their activity. In particular, these enzymes are involved in different pathways of detoxification, oxidative stress defence and energetic metabolism. The analysis of different biochemical indicators highlighted some interesting physiological variations that can be linked to the pesticide exposure. We therefore stress the attention on the possibility of using such a methodology as a novel toxicity endpoint in environmental risk assessment.

Submitted by Johnny Rodrigues (johnnyrodrigues@ufcg.edu.br) on 2018-05-17T18:15:59Z No. of bitstreams: 1 WHALAMYS LOURENÇO DE ARAÚJO - DISSERTAÇÃO PPGHT 2015..pdf: 998803 bytes, checksum: 00aca588e4e5182e7ebe10263ae94f8c (MD5) / Made available in DSpace on 2018-05-17T18:15:59Z (GMT). No. of bitstreams: 1 WHALAMYS LOURENÇO DE ARAÚJO - DISSERTAÇÃO PPGHT 2015..pdf: 998803 bytes, checksum: 00aca588e4e5182e7ebe10263ae94f8c (MD5) Previous issue date: 2015 / Capes / O cultivo do meloeiro (Cucumis melo L.) é de grande importância para a economia brasileira. Seu cultivo tem demonstrado significativa expansão nas duas últimas décadas, especialmente nas regiões Semiáridas do Nordeste. Apesar dos fatores favoráveis à produtividade do meloeiro, vale salientar fatores limitantes, destacando-se os danos ocasionados por pragas que destroem o cultivo e, consequentemente, diminuindo a colheita, obrigando que medidas de controle sejam adotadas. Entres estas, estão aplicações de inseticidas químicos, entre eles os neonicotinoides. Estudos têm demonstrado os danos colaterais destes inseticidas a insetos benéficos como abelhas, que por sua vez são de fundamental importância para a polinização de 90% das angiospermas, principalmente para o melão. Este trabalho teve como objetivo avaliar a toxicidade de neonicotinoides empregados para o controle de pragas na cultura do meloeiro à abelhas A. mellifera Linnaeus. Os bioensaios foram realizados em laboratório, sendo tomadas medidas repetidas no tempo de mortalidade para os produtos comerciais Actara 250 WG (tiametoxam), Evidence 700 WG (imidacloprid), Mospilan (acetamipride) e Orfeu (acetamipride). A exposição das abelhas aos compostos foi realizada por meio de pulverização e ingestão de alimento contaminado com as maiores e menores doses recomendadas pelos fabricantes. Independente do modo de exposição, tiametoxam, imidaclopride e acetamipride (duas formulações) foram tóxicos, diminuindo o tempo de vida útil das abelhas em até 1, 2, 11 e 11 dias, respectivamente, quando comparados com a testemunha, que por sua vez sobreviveram 18 dias, em média. O inseticida tiametoxam foi extremamente tóxico quando pulverizado sobre as abelhas, bem como quando contaminante da dieta ingerida. O imidaclopride também apresentou toxicidade em ambos os experimentos, sendo depois do tiametoxam o mais tóxicos. O inseticida acetamipride apresentou toxicidade, porém não tão alta, quanto às observadas com o tiametoxam e o imidaclopride. / The cultivation of melon (Cucumis melo L.) is of great importance for the Brazilian economy, especially in semiarid regions of the Northeast. Despite the favorable factors to the yield of melon other limiting factors have been highlighted as the damage caused by pests, which undertake to harvest, requiring that control measures are adopted. Among these are applications of chemical insecticides, including the neonicotinoids. Studies have shown the collateral damage of these insecticides to beneficial insects like bees, important pollinators of 90% of angiosperms, mainly to melon. The objective of this study was to evaluate the toxicity of neonicotinoids used for pest control in the melon crop to Linnaeus honeybees. Bioassays were performed in the laboratory, being taken repeated measures in time to mortality commercial products Actara 250 WG (thiamethoxam), Evidence 700 WG (imidacloprid), Mospilan (acetamiprid) and Orpheus (acetamiprid) (two formulations). The exposure of bees to the compounds was performed by spraying and ingestion of food contaminated with higher and lower doses recommended by the manufacturers. Regardless of the exposure mode, all insecticides were toxic, reducing the useful life of bees within 1, 2 and 11 days, respectively, compared with the control (distilled water + pure candy), which survived 18 days, average . The insecticide thiamethoxam in both experiments and doses, was extremely toxic; as well as Imidacloprid, the second most toxic. The Acetamiprid insecticide, in both formulations showed toxicity, but not so high as those observed with other insecticides in the study.