<p>The aim of this work was to understand the molecular mechanism of translation and the mechanism of translation termination, in particular. Cleavage of peptidyl-tRNA and peptide release terminates translation of mRNA on the ribosome. In prokaryotes, three release factors (RFs) are involved in this process. RF1 and RF2 recognise the three stop codons on mRNA and induce hydrolysis of the ester bond in peptidyl-tRNA. RF3 accelerates the rate of RF1 and RF2 recycling between ribosome in a GTP-dependent manner. We have clarified the mechanism of action of peptide release factor RF3. In the cell, free RF3 is in the GDP conformation. When RF3∙GDP binds to ribosome in complex with RF1 or RF2, these ribosome complexes act as guanine exchange factors for RF3 by inducing rapid dissociation of GDP. If, and only if, the peptide has been removed from tRNA, GDP is quickly replaced by GTP. Binding of GTP to RF3 induces a conformation of the factor with high affinity for the ribosome, which forces RF1 or RF2 to rapidly dissociate. Subsequent hydrolysis of GTP on RF3 induces a factor conformation with low affinity for the ribosome and rapid release of RF3∙GDP. It was further shown how the position of peptidyl-tRNA on the ribosome and the presence or absence of its peptide regulates the binding and GTPase activity of translation factors IF2, EF-G and EF-Tu. The result explains how idling GTPase hydrolysis and negative interference between different translation factors are minimized in living cells. The present biochemical observations, in conjunction with cryo-EM results, lead to new proposals for the role of hybrid sites in translocation of tRNAs, recycling of RF1 and RF2 by RF3 and recycling of post-termination ribosomes back to a new round of initiation.</p>

The aim of this work was to understand the molecular mechanism of translation and the mechanism of translation termination, in particular. Cleavage of peptidyl-tRNA and peptide release terminates translation of mRNA on the ribosome. In prokaryotes, three release factors (RFs) are involved in this process. RF1 and RF2 recognise the three stop codons on mRNA and induce hydrolysis of the ester bond in peptidyl-tRNA. RF3 accelerates the rate of RF1 and RF2 recycling between ribosome in a GTP-dependent manner. We have clarified the mechanism of action of peptide release factor RF3. In the cell, free RF3 is in the GDP conformation. When RF3∙GDP binds to ribosome in complex with RF1 or RF2, these ribosome complexes act as guanine exchange factors for RF3 by inducing rapid dissociation of GDP. If, and only if, the peptide has been removed from tRNA, GDP is quickly replaced by GTP. Binding of GTP to RF3 induces a conformation of the factor with high affinity for the ribosome, which forces RF1 or RF2 to rapidly dissociate. Subsequent hydrolysis of GTP on RF3 induces a factor conformation with low affinity for the ribosome and rapid release of RF3∙GDP. It was further shown how the position of peptidyl-tRNA on the ribosome and the presence or absence of its peptide regulates the binding and GTPase activity of translation factors IF2, EF-G and EF-Tu. The result explains how idling GTPase hydrolysis and negative interference between different translation factors are minimized in living cells. The present biochemical observations, in conjunction with cryo-EM results, lead to new proposals for the role of hybrid sites in translocation of tRNAs, recycling of RF1 and RF2 by RF3 and recycling of post-termination ribosomes back to a new round of initiation.

Thesis (M.A.I.S.)--Oregon State University, 2010. / Printout. Includes bibliographical references (leaves 99 - 103). Also available on the World Wide Web.

Having spent over 20 years under house arrest fighting for democracy in Myanmar, Aung San Suu Kyi has been a bastion for peace for decades. She has received many international awards, including the Nobel Peace Prize in 1991, which she accepted in person in 2012. The plight of the Rohingya, a Muslim minority in Myanmar, has marred Suu Kyi’s reputation as a bastion of peace, leading to calls for her to lose her Peace Prize. Why is it that Suu Kyi’s image as the future of peace so different from reality? That question is what this research attempts to answer. Through a rhetorical analysis of Suu Kyi’s Nobel lecture and the media coverage that followed it, the impact of the use of tropes becomes evident. Metonymy, synecdoche, and narrative emerge in both the lecture and media coverage. Suu Kyi’s use of tropes heavily influences public perception of her.

Thesis (Honors)--College of William and Mary, 2008. / Includes bibliographical references (leaves 60-64). Also available via the World Wide Web.

El Premio Nobel de Química de 2013 ha sido otorgado a A. Warshel, M. Levitt y M. Karplus debido al desarrollo de métodos híbridos de cálculo para química computacional. En este artículo se presentará una breve introducción del uso de los métodos de química computacional. Se describirá cómo se desarrollaron, y por qué, los métodos híbridos de cálculo, conocidos como QM/MM (Quantum Mechanics/Molecular Mechanics) para el estudio de sistemas macromoleculares, sobre todo para el caso de su aplicación en enzimas y bioquímica. Finalmente, se comentarán los alcances y expectativas futuras para estos métodos, desarrollados en los años 70. / The 2013 Nobel Prize in chemistry was awarded to A.Warshel, M.Levitt and M.Karplus for their contribution to the development of hybrid methods for computational chemistry. In this article a brief introduction about computational chemistry methods is presented. This paper will show the order in which the QM/MM (Quantum Mechanics/Molecular Mechanics) methods were developed for the study of macromolecular systems and specially their application in enzymes and biochemistry. Finally, the reach and future prospects of these methods originally developed by A. Warshel, M.Levitt and M. Karplus in the seventies will be discussed.

The purpose of this essay is to investigate how the Nobel Committee uses the concept of peace in their press releases about the Nobel Peace Prize. This essay starts by investigating various articles and books to establish a basic understanding of how to efficiently investigate peace. This research then prompts us to investigate the related concepts of War, Conflict and Violence. It also tasks us to investigate a possible expansion of the concept in the context of a expanding globalised world. We could also relate ideas of peace to ideas of democracy and world order. This is done through Kosellecks methods of ”begriffsgeschichte”, by investigating the use of these various terms as well as their semantic field, we get an idea of how they’re conceived, and thus, the context in which they are used. This essay uses press releases found on the Nobel Prize Committee website as well as the award speeches as complements. This essay shows us how the concept of peace has grown, incorperating new ideas of justice, democracy and human rights. This can be seen in the context of an increasingly complex, westernized, globalised world, furthering the need for wider, more nebulous concepts to describe it.

(in English): This thesis deals with a version of Boris Pasternak's novel Doctor Zhivago, translated into Czech by Jan Zábrana and Jiří Kovtun. The aims of the thesis are: (1) to follow the journey of the original exile edition from Italy to communist Czechoslovakia, (2) to clarify how both translators contributed to the poetry part of the novel, (3) to outline Zábrana's translation method a comparison of chosen typewritten versions of the translation from various phases of its creation. The primary source of material for all the individual objectives is Zábrana's inheritance, which answers the question why Jiří Kovtun's name does not appear in the first Czech edition of Doctor Zhivago (1990), although he is listed along with Zábrana as the translator of the poetry part in the remaining three editions (2003, 2005, 2011). The original material from the inheritance as well as Marie Zábranová's testimony emphasize the sequence of events that influenced the creation of Zábrana's translation. The final analysis compares chosen typewritten versions of the novel's eighth chapter with the first Czech edition and describes how Zábrana worked. The thesis aims to be beneficial for the Czech history of translation and contribute to the current knowledge about the translation method of Jan Zábrana. Keywords Jan...

Dominant market theories analyze markets as ahistorical entities without the need for professional groups that manage crucial functions within them. This thesis, in contrast, approaches markets as historical systems that develop over time and that can be constituted in many different ways because of different historical trajectories. Different professional groups managing market routines, further, are seen as a crucial part of markets. Two concepts are introduced: “market architecture”, the specific way a market is constituted at a given time; and “market technocrats”, the seemingly disinterested third party functionaries that manage routines in markets and advocate changes in market architecture. The thesis argues that market technocrats exist because of uncertainty and lack of trust between market actors, and that they are an important part of how market architectures develop over time. It presents an analytical framework for understanding market technocrats and how they interact with and develop markets. Four different aspects of market technocrats are explored: the process of establishing market technocrats in market routines; the capture of the authority of market technocrats by other market actors; the expansionistic behavior of market technocrats; and the way changes in economic theory, as an important part of how economists with technocratic authority advocate market change, can help to explain changes in markets. These aspects are explored through four empirical papers: The Market Technocracy of Import Substitution: The Role of Asymmetric Information and The Swedish Seed Association 1880–1935; Limits of Market Technocracy: Swedish Fertilizer Research and the Crisis of Objectivity 1945–1960; Central Banks, and the Pursuit of Influence, Prestige, and Legitimacy: The Creation of the Nobel Memorial Prize; and From Market Engineering to Institutional Engineering: Reform Economics in Sweden 1950–2000. The results of the papers form the basis of a hypothetical narrative of how the role of market technocrats has changed during the 20th century. This provides a roadmap for further research in the development of markets and the role of market technocrats.

Neste trabalho discutimos aspectos de natureza da ciência presentes em discursos proferidos por ganhadores do Prêmio Nobel. Analisamos quatro palestras utilizando metodologia apoiada na Análise Textual Discursiva; as palestras analisadas foram as de Marie Curie proferida em 1911, Chandrasekhara Venkata Raman em 1930, Alexander Fleming em 1945 e Richard Feynman em 1965. A escolha deu-se pela popularidade desses cientistas segundo o website oficial da premiação. Os laureados são apresentados contextualmente considerando os contextos epistemológicos, sociais e culturais de cada período. Baseamo-nos na abordagem de ciência integral para analisar as fontes primárias em busca de conceitos relacionados a natureza da ciência. Com isso, temos por objetivo fornecer subsídios para que educadores científicos em formação tenham à disposição uma metodologia confiável para introduzir discussões de natureza da ciência em sala de aula, utilizando-se de fontes históricas primárias. A problematização de uma instituição de tamanha importância quanto o Prêmio Nobel é relevante para um ensino de ciências que tenha uma abordagem histórica, social e epistemológica. Por meio de fontes históricas primárias, podem ser explicitados e problematizados conceitos sobre como se desenvolve e se constrói o fazer científico em nossa sociedade. / We discuss Nature of Science aspects presented in the official Nobel Lectures. We analyze 4 lectures using a methodology which is based on Discursive Textual Analysis: Marie Curie\"s in 1911, Chandrasekhara Venkata Raman\"s in 1930, Alexander Fleming\"s in 1945 and Richard Feynman\"s in 1965. These lectures were selected based on the laureates\" popularity among general public according to the official Nobel website. Laureates are presented considering epistemological, social and cultural contexts in each period. The whole science approach is used in order to read the primary sources seeking concepts related to nature of science. Our aim is to provide trustful methodologies and sources directed to undergraduated scientific educators, in order to introduce discussions on nature of science in classroom. It is relevant to approach critically such important institutions to the sciences as the Nobel Prize in a teaching program that envises an historical, social and epistemological approach. By using primary historical sources, we can foster discussions about concepts on how science is developed and constructed in our society.