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The purpose of this study was to observe penetration profiles of lysozyme on a variety of contact lens materials by confocal microscopy, to analyze influential factors that are involved in these penetration curves and to suggest possible mechanisms related to the in-eye clinical performance of these materials. An FITC-lysozyme conjugate was synthesized in-house by amine reaction. Contact lenses were incubated in a lysozyme solution with a final concentration of 1.9 mg/mL for various periods before undergoing microscopic analysis. Optimal parameters for confocal scanning were successfully obtained to acquire desired fluorescence signals on various contact lenses. Measurement units were converted into absolute amounts of lysozyme using lysozyme data from ^(125)I gamma counting studies. A rhodamine labeled dextran solution was applied to distingush the surface of the contact lenses under examination. The data from these studies were then used to calculate the theoretical numbers of layers of adsorbed lysozyme on the lens surface. The results show that there were distinct differences in lysozyme penetration in the twelve hydrogel materials examined. A pure pHEMA lens, with a water content of 38%, deposited lysozyme primarily on the lens surface after 24 hours, with full penetration occurring after 4-weeks of incubation. Three types of non-ionic contact lens materials with water contents > 50% exibited rapid penetration within the lens bulk after 24-hours incubation, with increased deposition within the matrix after 4 weeks. Two ionic, high water content polymers (Acuvue 2 and Focus Monthly) exhibited markedly different penetration profiles, particularly after 24 hours, with very rapid and total penetration in Acuvue 2, as compared with partial penetration in Focus Monthly. Modern silicone hydrogel contact lenses can be nominally divided into first generation, plasma-modified materials and second generation materials which incorporate an internal wetting agent such as polyvinyl pyrrolidone (PVP). These materials exhibited different lysozyme deposition profiles. Lysozyme fully penetrated PureVision after 24 hours, whereas no lysozyme penetration occurred on lenses manufactured from Focus Night & Day or O_2Optix, even after 4 weeks. Lenses manufactured from Acuvue Advance and Acuvue OASYS, two second generation silicone hydrogel lenses, also displayed their own characteristic deposition profile. Acuvue Advance always exhibited a partial penetration of lysozyme within the matrix, even after 4 weeks of doping. Interestingly, Acuvue OASYS showed a similar profile to Focus Night & Day and O_2Optix, with predominantly surface deposition occurring. To confirm possible surface adsorption of lysozyme on surface-coated Focus Night & Day and O_2Optix, a rigid polymethylmethacrylate (PMMA) contact lens was used as a model of surface adsorption. A mounting medium containing rhodamine labeled dextran was scanned to distinguish the lens surface, as it was assumed that no surface penetration of the very high molecular weight dextran would occur. Using this model, it was confirmed that surface adsorption of lysozyme occurred on these plasmacoated lens materials, which is similar to that seen with PMMA. In a further experiment, it was seen that lysozyme sorption on Acuvue OASYS exhibits a penetration profile which is different to that seen in Focus Night & Day and O_2Optix, with lysozyme just penetrating the lens surface. The results from the studies described above demonstrated that in 24 hours lysozyme sorption did not achieve a complete monolayer. However, after 4 weeks multi-layer adsorption occurred, with the more hydrophilic materials depositing the most lysozyme. The quantitative measurement of lysozyme penetration on and into contact lens materials by confocal microscopy combined with ^(125)I labelling offers a valuable tool to discover the potential mechanisms of interactions between protein and polymer materials. This study reveals some important information that may be beneficial to contact lens development and will prove to be valuable in other more broad areas of biomedical research in which polymers and biological fluids come into contact. / Thesis / Master of Applied Science (MASc)

京都大学 / 新制・課程博士 / 博士(理学) / 甲第25095号 / 理博第5002号 / 新制||理||1714(附属図書館) / 京都大学大学院理学研究科数学・数理解析専攻 / (主査)教授 小野 薫, 教授 大木谷 耕司, 准教授 入江 慶 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

The focus of this study is on the development of a state-of-the-art single-wheel roller rig for studying contact mechanics and dynamics in railroad applications. The use of indoor-based simulation tools has become a mainstay in vehicle testing for the automotive and railroad industries. In contrast to field-testing, roller rigs offer a controlled laboratory environment that can provide a successful path for obtaining data on the mechanics and dynamics of railway systems for a variety of operating conditions. The idea to develop a laboratory test rig started from the observation that there is a need for better-developed testing fixtures capable of accurately explaining the complex physics of wheel-rail contact toward designing faster, safer, and more efficient railway systems. A review of current roller rigs indicated that many desired functional requirements for studying contact mechanics currently are not available. Thus, the Virginia Tech Railway Technologies Laboratory (RTL) has embarked on a mission to develop a state-of-the-art testing facility that will allow experimental testing of contact mechanics in a dynamic, controlled, and consistent manner. VT roller rig will allow for closely replicating the boundary conditions of railroad wheel-rail contact via actively controlling all the wheel-rail interface degrees of freedom: cant angle, angle of attack, and lateral displacement. Two sophisticated independent drivelines are configured to precisely control the rotational speed of the wheels, and therefore their relative slip or creepage. A novel force measurement system, suitable for steel on steel contact, is configured to precisely measure the contact forces and moments at the contact patch. The control architecture is developed based on the SynqNet data acquisition system offered by Kollmorgen, the motors supplier. SynqNet provides a unified communication protocol between actuators, drives, and data acquisition system, hence eliminating data conversion among them. Various design analysis indicates that the rig successfully meets the set requirements: additional accuracy in measurements, and better control on the design of experiments. The test results show that the rig is capable of conducting various contact mechanics studies aimed for advancing the existing art. Beyond developing the experimental testing fixture for studying contact mechanics, this study provides a comprehensive review of the contact models. It discusses the simplifying assumptions for developing the models, compares the models functionality, and highlights the open areas that require further experimental and theoretical research. In addition, a multi-body dynamic model of the entire rig, using software package SIMPACK, is developed for conducting modal analysis of the rig and evaluating the performance of the rig's components. A MATLAB routine is also developed that provides a benchmark for developing creep curves from measurements of the rig and comparing them with existing creep curves. / Ph. D.

Despite the lack of consequences for not voting, many Americans do consider it a duty to participate in elections, with the exception of American youth, who have habitually failed to participate. In this study, the efficacy of contact among youth voters is studied as it relates to Election Day turnout and vote choice. Although political parties continue to exhaust vast resources on contact in an effort to mobilize the youth, it was not until the 2008 Presidential Election that American youth showed a significant increase in turnout. Rather than continue to expend resources on forms of contact that do not impact the cohort that most needs a method of mobilization, the useful forms can be identified and employed in the present and future. To determine which traditional form or forms of contact have the greatest positive impact on American youth, data from the 2008 American National Election Study is analyzed. In addition, to determine which new types of technology will most likely be useful in the future of youth mobilization, data was gathered from a sample set of 100 college students. The data from the 2008 ANES determined that Young Democrats were most highly affected by contact in terms of voter turnout. The affect of contact among young voters is to gain a larger portion of their vote than amongst older voters, but the Democratic Party stands out as the party most successful in doing so. Contact proved to have a positive effect on the people who need it least, those who already identify with a political party, and the least positive effect on those who need it most. Change in the methodology of contact alone will not be successful in getting these people to share their political beliefs, learn about candidates and parties, or even to vote on Election Day.