This master thesis is focused on the delays incurred in data transmitting over IP networks. The top of the paper is devoted to points, where delay is invoked and causes of the latency. For individual sources of delay are are described the parameters that affect the usual size of latency. The next section describes the types of networks and their typical delay. In the paper are also discussed tools for measuring delay and determining the number of intermediate devices. The thesis contains results of measurements delay time in the real network. For these results are listed conclusions about the delay for one intermediate device. There is also said general ratio of crow-fly distance and calculated distance. The last part deals the simulation of delay in the NS2 program. The results of thesis are scripts that shows the relation of delay and the number of switches, routers, and the length of the path.

All viruses encode for at least one viral interferon (IFN) antagonist, which is used to subvert the cellular IFN response, a powerful antiviral innate immune response. Numerous in vitro and in vivo studies have demonstrated that IFN antagonism is crucial for virus survival, suggesting that viral IFN antagonists could represent promising therapeutic targets. This study focuses on Respiratory Syncytial Virus (RSV), an important human pathogen for which there is no vaccine or virus-specific antiviral drug. RSV encodes two IFN antagonists NS1 and NS2, which play a critical role in RSV replication and pathogenicity. We developed a high-throughput screening (HTS) assay to target NS2 via our A549.pr(ISRE)GFP-RSV/NS2 cell-line, which contains a GFP gene under the control of an IFN-stimulated response element (ISRE) to monitor IFN- signalling pathway. NS2 inhibits the IFN-signalling pathway and hence GFP expression in the A549.pr(ISRE)GFP-RSV/NS2 cell-line by mediating STAT2 degradation. Using a HTS approach, we screened 16,000 compounds to identify small molecules that inhibit NS2 function and therefore relinquish the NS2 imposed block to IFN-signalling, leading to restoration of GFP expression. A total of twenty-eight hits were identified; elimination of false positives left eight hits, four of which (AV-14, -16, -18, -19) are the most promising. These four hit compounds have EC₅₀ values in the single μM range and three of them (AV-14, -16, -18) represent a chemically related series with an indole structure. We demonstrated that the hit compounds specifically inhibit the STAT2 degradation function of NS2, not the function of NS1 or unrelated viral IFN antagonists. At the current time, compounds do not restrict RSV replication in vitro, hence hit optimization is required to improve their potency. Nonetheless, these compounds could be used as chemical tools to determine the unknown mechanism by which NS2 mediates STAT2 degradation and tackle fundamental questions about RSV biology.

近十年來，由於無線網路的普及與人們對於影像串流服務的需求愈高，導致人們迫切需要更好的服務品質。但在IEEE 802.11無線網路中，本身的設計並非針對影像串流來設計，為讓影像串流能在無線網路更有效率，我們必須重新設計適合影像串流的無線網路。本研究首先探討IEEE 802.11 MAC層DCF (Distributed Coordination Function)與802.11e EDCA (Enhanced Distributed Channel Access)機制的潛在問題。由於DCF與EDCA並未特別對影像串流做設計，會導致具有時延(delay time)限制的影像封包等待過久造成失效，卻仍繼續傳送。本研究提出幾個有效方法，改善原本IEEE 802.11無線網路對影像串流傳輸效能不彰的現象。我們將利用跨層設計使MAC層能取得影像串流封包資訊，並改善DCF與EDCA的重傳(retransmission)機制，使用time limit與retry limit混和設計節省不必要的等待時間，並使用single-video multi-level queue改善傳輸效能。最後本研究將利用網路模擬器NS-2 (Network Simulater ver. 2)與影像串流測試實驗架構myEvalvid-NT作不同效能的驗證比較並評估我們所提出方法的有效性。 / Over the past decade, wireless network access and video streaming services have become more popular than ever. People are eager to have better quality of video streaming services over wireless network. However, IEEE 802.11 DCF and IEEE 802.11e EDCA are not specifically designed for video streaming. This leads to the problem of transmitting overdue video packets and thus degrades both the network performance and video quality. In this paper, we propose a hybrid design framework to improve the quality of video streaming. This framework consists of a MAC-centric cross-layer architecture to allow MAC-layer to retrieve video streaming packet information (slice type and transmission deadline), a retransmission mechanism of hybrid retransmission deadline and retry limit to save unnecessary packet waiting time, and a single-video multi-level queue to prioritize I/P/B slice delivery. Simulations show that the proposed methodology outperforms IEEE 802.11e, IEEE 802.11e Timebase and IEEE 802.11e MultiQ in packet loss rate, invalid packet ratio, lost and invalid packet ratio, delay time, jitter, and PSNR.

There is information to my master's thesis which is called “Web application for NS2 training”. This application works after installation and its source codes are saved on applied CD. It is said about implement Network Simulator 2. It helps to realize simulation of nets and then author inserts information about them into web application. Registered web's visitor has possibility to insert project into application. The project contents information about simulation created in NS2. Web application can also visible detail of possible project which is approved of administrator. Then the visitor can sort projects, search entered expression or connect his contribution to discussion forum. Administrator can approve users projects in his part of application. It makes available for the others. He can also delete them from database. Theory about technologies which are used for implementation of this application. It is talked about web Apache server, database MySQL server and programmable PHP language. There is also mentioned information about security of web application included possible attacks on applications and their database. It is presented proposal of database which creates core of application. This proposal is depended on application requirements. Next chapters give to reader whole image about functionality of application. There are mentioned samples of final graphical image of application. This document also provides the shows of source codes for creating database tables.