Proefschrift--Rijksuniversiteit te Leiden.

Thesis (Ph. D.)--Brigham Young University. Dept. of Chemistry and Biochemistry, 2007. / Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1951. / Parts I.B., C., D., F., and II. A. reprinted from Journal of biological chemistry: Part I.B.: vol. 185, no. 2 (Aug. 1950), p. 741-747 ; Part I.C.: vol. 187, no. 2 (Dec. 1950), p. 597-604 ; Part I.D.: vol. 189, no. 2 (Apr. 1951), p. 659-663 ; Part I.F.: vol. 189, no. 1 (Mar. 1951), p. 361-369 ; Part II. A.: vol. 185, no. 2 (Aug. 1950), p. 887-893. Remainder typescript. Vita. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1968. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--University of Missouri-Columbia, 2005. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file viewed on (January 22, 2007) Includes bibliographical references.

Two kinds of affinity chromatography, CDP-choline- and CTP-Sepharose 4B, were investigated for purification of the cytosolic CTP:phosphocholine cytidylyltransferase from rat liver. The enzyme did not show strong affinity for the CDP-choline Sepharose resin, but bound to the CTP-Sepharose column in the presence of 14 mM magnesium acetate. The combination of CTP affinity chromatography with ion-exchange techniques provided about 70-fold purification of the cytosolic enzyme with a specific activity of about 90 units per milligram protein. The influence of diphenylsulfone compounds on the synthesis of phosphatidylcholine by the CDP-choline pathway was examined in isolated rat hepatocytes and HeLa cells. The administration of the sulfones (100 ug/ml), except dapsone, to HeLa cells inhibited the total [methyl-³H]choline incorporation into the cells, but did not change the rate of conversion of choline to phosphatidylcholine. The addition of the sulfones (100 ug/ml) to rat hepatocytes did not inhibit the biosynthesis of phosphatidylcholine and choline metabolism. The effect of vasopressin on the distribution of cytidylyl-transferase between cytosol and microsomes in rat hepatocytes was also investigated. The digitonin-mediated release of cytosolic cytidylyltransferase was reduced from the cells treated with vasopressin (5-20 nM) , while the enhanced rate of incorporation of [methyl-³H]choline into phosphatidylcholine was not observed. / Medicine, Faculty of / Biochemistry and Molecular Biology, Department of / Graduate

A dissertation submitted to the Faculty of Health Sciences, Umversity of the Witwatersrand, Johannesburg, for the degree of Masters of Science in Medicine (Virology). Johannesburg, 2001 / This dissertation examines HIV-1 subtype C nucleotide and amino acid Nef sequences from South African recently-infected (n=12) and chronically-infected individuals (n=9) and from AIDS patients (n=ll). The overall aim is to determine which regions of subtype C Nef are variable and which are conserved and to associate these regions with targeting by the immune system. Phylogenetic analysis and sequence alignments showed that there was no association of Nef variations with stage of disease. Nef amino acid alignments of 32 sequences showed a high degree of conservation in areas of functional and structural importance. The mean intrasubtype variation from these isolates was 15.8%, with sequences from the AIDS cohort being significantly (p<0.05) more variable than those from recent or chronic infection / IT2019

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Pyrimidine nucleotide biosynthesis was studies in Acinetobacter calcoaceticus ADP-1. Pyrimidine auxotrophic mutants were isolated and characterized for this purpose. One such Pyr mutant, strain ADP-1-218 was chosen for further study.