The objective of this research is to investigate the use of laser direct writing to micro-pattern low loss passive optical channel waveguide devices using a new hybrid organic/inorganic polymer. Review of literature shows previous methods of optical waveguide device patterning as well as application of other non-polymer materials. System setup and design of the waveguide components are discussed. Results show that laser direct writing of the hybrid polymer produce single mode interconnects with a loss of less 1dB/cm.

Anemi kan uppstå till följd av förlust av erytrocyter eller försämrad produktion av nya erytrocyter. För att upptäcka och följa upp patienter med anemi är det viktigt att korrekt kunna mäta hemoglobinkoncentrationen (Hb) i blodet. En vanlig metod för att mäta Hb-koncentration är fotometri i kombination med en kemisk omvandling. Liksom alla mätningar med ljus är dessa känsliga för turbiditet i provet. Lipemi är en vanlig källa till turbiditet som kan uppstå till exempel som följd av en fettrik måltid, diabetes mellitus, lever- eller njursjukdomar, alkoholism och vissa läkemedel. Olika instrumenttillverkare har olika metoder för att motverka interferensen av lipemi. Sysmex hematologi-instrument XN-10 använder en fettlösande bärarvätska i sin fotometriska kanal (HGB) och HemoCue mäter vid en andra våglängd som ska kompensera för turbiditet. Sysmex XN-10 har också en optisk kanal (HGB-O) som är till för att räkna retikulocyter genom att mäta deras nukleinsyra- samt Hb-innehåll men ger då också ett beräknat värde på Hb-koncentrationen i hela provet. Syftet med denna studie var att jämföra HGB och den HGB-O för bestämning av Hb-koncentrationen i helblod. Båda kanalerna jämfördes även med HemoCue Hb 201+ vid bestämning av Hbkoncentrationen i svårt lipemiska prover. Hb-mätning på plasma från motsvarande prover utfördes också för att undersöka om värdet motsvarade Hb-höjningen i de lipemiska proverna. Prover analyserade med både HGB och HGB-O på Sysmex XN-10 på klinisk kemi vid Skånes universitetssjukhus i Lund under november månad 2018 (n = 392) jämfördes med hjälp av Spearmans rangkorrelationskoefficient. Lipemi simulerades med fettemulsionen Intralipid i totalt 32 prover. Färdiganalyserade patientprover från föregående dag delades i ett lipemiskt prov med Intralipidtillsats och ett nollprov med tillsats av NaCl-lösning i en motsvarande volym. Differenserna mellan de lipemiska- och nollprovernas Hb-värden testades för signifikans med icke-parametrisk Wilcoxons teckenrangtest. Kruskal-Wallis samt Dunns's tester användes för att visa på signifikanta skillnader mellan de tre metoderna. Signifikansnivån sattes vid p < 0,05. Resultaten visade god korrelation mellan HGB - och HGB-O Hb-värden med ett Spearman korrelationsvärde på 0,982.  Jämförelsen av metoderna vid lipemi visade signifikant skillnad mellan nollprov och lipemiskt prov för HGB- (p < 0,001) men inte HGB-O (p = 0,11) på XN-10. HemoCue Hb 201+ visade också signifikant skillnad (p < 0,001) vid lipemi men med lägre median-värde än HGB och mindre spridning än HGB-O. HGB-O:s median-värde tydde på minst lipemipåverkan men spridningen av differenserna var stor. Spridningen av HGB-O resultaten kan bero på hemolys då endast intracellulärt Hb mäts i denna kanal. Resultaten i denna studie tyder på att HemoCue-metoden är den mest pålitliga vid Hb-mätning av lipemiska prover och därmed det lämpligaste komplementet till HGBmetoden / Anemia can arise from either loss of erythrocytes or impaired production of new erythrocytes. In order to discover and evaluate the treatment of anemic patients, correct Hb measurements are important. A common method to measure Hb concentration is photometry in combination with chemical conversion of the Hb. Like all light-dependent methods this suffers from a vulnerability to turbidity that scatters light. Lipemia is a common cause of turbidity caused by e.g. recent intake of high fat foods, diabetes mellitus, liver or kidney disease, alcoholism and some drugs. Manufacturers of Hb analyzers use different methods to counter the influence of interference from lipemia on measurements. Sysmex XN-10 analyzers use a fat dissolving sheath fluid in its photometric channel (HGB) and HemoCue measures absorbance at a second wavelength to compensate for turbidity. Sysmex XN-10 also has an optic channel (HGB-O) for counting reticulocytes by measuring their nucleic acid and Hb content. At the same time this channel measures Hb equivalents of erythrocytes and gives a calculated value of Hb content in the entire sample. The aim of this study was to compare the photometric and the optical channels for measuring Hb concentration in whole blood. Both the Sysmex XN-10 channels were compared with HemoCue Hb 201+ when measuring Hb concentrations in lipemic samples. Plasma Hb concentration was determined for the corresponding samples in order to investigate correlation between elevation in Hb concentration with and without simulated lipemia and in the plasma after centrifugation. Samples analyzed at Skånes University Hospital in Lund during the month of November 2018 (n = 392) using both HGB and HGB-O on XN-10 were compared using Spearman's signed correlations coefficient. Lipemia was simulated by using the fat emulsion Intralipid in a total of 32 samples. Samples collected and analyzed on the previous day was used for the study. Each sample was split into one part with added Intralipid to form a lipemic sample and one part with NaCl-solution of the same volume as Intralipid in the lipemic sample. The differences between lipemic and non lipemic samples was tested for significance by the non-parametric Wilcoxons signed ranks test for each of the methods. Significance between the three methods was tested by using Kruskal-Wallis and Dunn's tests. Level of significance was set to p < 0.05. The results showed good correlation between earlier test run on both HGB and HGB-O with a Spearman correlation score of 0,982.  A significant difference was found between lipemic and non lipemic samples with the photometric method (p < 0,001) but not the optical method (p = 0,11) on XN10. HemoCue Hb 201+ also showed a significant difference (p < 0,001) between lipemic and non lipemic samples but a lower median than HGB and less deviation than HGB-O. The median of HGB-O indicated that it was influenced the least by lipemia of the three methods but had the greatest deviation of the differences. The greater deviation of HGB-O values may have been caused by hemolysis since the method measures intra cellular Hb. HemoCue shows according to this study the slightest deviation of the three methods and a less heightened median value compared to HGB which confirms the methods suitability as complement to HGB when dealing with lipemic samples.

Cette thèse s’intéresse à l’utilisation de techniques de communications bidirectionnelles par optique sans fil pour le suivi à distance des données médicales d’un patient. Plus précisément, un lien infrarouge est considéré pour la voie montante entre un dispositif porté par le patient et plusieurs récepteurs disposés au plafond. Pour la voie descendante, on étudie un lien en lumière visible, lié aux sources d’éclairage de l’environnement. Afin d’illustrer le potentiel de la technologie pour ce type d’application, la contribution principale est l’étude des performances des canaux de communication infrarouge et visible en considérant le fait que le dispositif est porté par un patient en mouvement dans l’environnement. Après la description générale des communications par optique sans fil, les principes de base et les différentes caractéristiques sont plus particulièrement détaillés dans le domaine du visible dont le composant clé est la source LED pour la double fonction d’éclairage et de communication. La méthode utilisée pour la simulation du canal optique sans fil est basée sur un logiciel de lancer de rayons associé à la technique de Monte-Carlo qui permet une modélisation complexe du scénario de télésurveillance. L’étude du canal en visible a ainsi montré que pour prédire les performances avec fiabilité, il était important de modéliser la présence du corps du patient ainsi que les variations induites par son mouvement sur la position et l’orientation du dispositif porté. De plus, l’impact des propriétés de réflectivité optique du corps ne peut être négligé. La performance globale du lien bidirectionnel Infrarouge/Visible a ensuite été discutée en termes de probabilité de rupture pour les différentes contraintes en débit et en qualité de service associées à la plupart des applications médicales. Les différents résultats illustrent la faisabilité du scénario étudié et donc le potentiel de l’optique sans fil en alternative aux radiofréquences pour des applications de télésurveillance médicale. Pour conclure, des expérimentations en infrarouge permettent de valider les résultats du lien montant. Pour le lien descendant, un premier banc expérimental est présenté, permettant d’envisager de nombreuses perspectives. / This thesis focuses on bidirectional optical wireless communication techniques for the indoor monitoring of medical patient data. More precisely, the uplink uses infrared transmissions between a device carried by the patient and several receivers dispatched on the ceiling. For the downlink, the study deals with Visible Light Communications (VLC) using the lighting of the environment. In order to illustrate the potential of this technology for medical monitoring, the main contribution of this study consists in the evaluation of the infrared and visible channel performance considering a wearable device and patient mobility in the environment. After a general description of optical wireless communications, the thesis focuses on basic principles and characteristics of VLC, in particular the LED which is the key component allowing to provide both lighting and communication. The method used for the optical channel simulation is based on ray-tracing method associated with Monte-Carlo technique permitting a complex modeling of the studied scenario. From VLC channel study, it is shown that in order to carefully predict performance, it is important to model the presence of the patient’s body and to consider the variations induced by its movement on the position and the orientation of the wearable device. Moreover, the impact of body’s reflectivity optical properties cannot be neglected. The overall performance of the Infrared/Visible bidirectional link is then discussed in terms of outage probability for several data rates and qualities of services corresponding to several medical data types. The results highlight the feasibility of the considered monitoring scenario and the potential of optical wireless communication as an alternative for radiofrequencies regarding remote transmission. To conclude, some experimentations contribute to validation for the infrared uplink. Finally, the development of an experimental test bed on the visible downlink opens the way for future tests in order to validate the overall theoretical performances.

Made available in DSpace on 2016-04-04T18:31:45Z (GMT). No. of bitstreams: 1 RICARDO VICENTE FAVERO.pdf: 1635235 bytes, checksum: d51f441103ff9f2ad94576b0bdd11b9f (MD5) Previous issue date: 2015-11-13 / The wavelength division multiplexing (WDM) optical network accommodates traffic load in 100, 50 and 25 GHz fixed-grid channel. This fixed-grid condition limits the number of lightpath for each optical fiber (80 channels in c-band) and doesn t allow bit rates with bandwidth over 50 GHz. To improve these factors, the flexibly grid elastic optical network (EON) was proposed, aiming accommodate adequately bit rates demand by customers. This proposal allows efficiency bandwidth and also expands bit rates supported by network. The EON bandwidth efficiency is obtained by routing and spectrum assignment (RSA) algorithm which acts to maximize the bandwidth utilization. Even with RSA, EON still show fragmentation rates substantial. In this context, this work proposes a new elastic optical network defragmentation strategy. This defragmentation strategy selects the lightpaths from the most fragmented link. The defragmentation process is based on RSA (DF-RSA). The DF-RSA determines the new position to reallocate the connection selected and performs. Using computer simulation of EON operation, were submitted several bit rates demands with different modulations format and traffic load between 45 and 100 erlang. Two simulation scenarios were proposed. The first one, compare the performance of RSA algorithm first-fit (FF) with and without defragmentation. It was considered as defragmentation process beginning point (trigger), the number of release connections. This scenario had until 48% of relative gain on minimizing blocking probability. The second scenario compared the performance of the follows RSA algorithms: FF, Maximize Path Spectrum Consecutiveness (MPSC) and Fragmentation Aware (FA). The FF was evaluated with and without defragmentation process and the others just with defragmentation process. The trigger employed was eventual connection blocked. The second scenario reached over the 80% blocking probability relative gain in 50 erlang traffic load. We conclude that the new elastic optical network defragmentation offers substantial gain bandwidth utilization and consequently blocking probability reduction. / As redes ?pticas de multiplexa??o por divis?o de comprimento de onda (WDM) acomodam o tr?fego em canais fixos de 100, 50 e 25 GHz. Esta condi??o de grade fixa limita o n?mero de conex?es por fibra ?ptica (80 canais na banda C), e n?o permite taxas de transmiss?o com ocupa??o espectral acima de 50 GHz. Para melhorar estes fatores, foram propostas as redes ?pticas el?sticas (EON) com canais flex?veis, visando acomodar adequadamente as taxas de transmiss?o demandas pelos usu?rios. Esta proposta possibilita maior efici?ncia espectral e tamb?m amplia as taxas de transmiss?o suportadas pela rede. A efici?ncia espectral nas EONs ? obtida com os algoritmos de roteamento e atribui??o espectral (Routing and Spectrum Assignment, RSA), que atuam para maximizar seu uso espectral. Mesmo com o uso de RSAs, as EONs ainda apresentam ?ndices de fragmenta??o consider?veis. Neste contexto, este trabalho prop?e uma nova estrat?gia de desfragmenta??o espectral para EONs. Esta proposta de desfragmenta??o seleciona as conex?es do enlace mais fragmentado, para o processo de desfragmenta??o. A desfragmenta??o baseia seu processo de realoca??o de conex?es por RSA, denominado DF-RSA. O DF-RSA determina a nova posi??o e realiza a realoca??o das conex?es. Com o uso de simula??o computacional da opera??o de funcionamento da EON, foram submetidas v?rias demandas de taxas de transmiss?o com diferentes modula??es e cargas de tr?fego entre 45 e 100 erlang. Foram propostos dois cen?rios de simula??o. No primeiro, foi comparado o desempenho do algoritmo RSA First-Fit (FF) com e sem o processo de desfragmenta??o. Considerou-se como ponto de inicio das desfragmenta??es (gatilho), o n?mero de conex?es liberadas da rede. Neste cen?rio obteve-se at? 48% de ganho relativo na minimiza??o da probabilidade de bloqueio. No segundo cen?rio, foram comparados os desempenhos dos seguintes algoritmos RSAs: FF, Maximize Path Spectrum Consecutiveness (MPSC) e Fragmentation Aware (FA). O FF foi avaliado com e sem desfragmenta??o e os demais somente com desfragmenta??o. Empregou-se como gatilho o eventual bloqueio de conex?o. O segundo cen?rio alcan?ou mais de 80% de ganho relativo de probabilidade de bloqueio para carga de tr?fego de 50 erlang. Conclui-se que a nova estrat?gia de desfragmenta??o para EONs oferece ganhos consider?veis na utiliza??o espectral e, consequentemente, redu??o na probabilidade de bloqueio.