Desoxyribonukleinsäure (DNS) ermöglicht die Selbstorganisation von nanoskopischen Elementen zu dreidimensionalen Einheiten mit vorgegebener Form, Zusammensetzung und Größe wie sie in der Nanoelektronik, Nanophotonik und Metamaterialien Verwendung finden. In dieser Arbeit werden DNS Origami Strukturen, in der Gestaltvon Nanoformen, Nanoblätchen und Nanoröhren, als Gerüste für den Aufbau von Nanodrähten und Metall/Halbleiter/Metall Heterostrukturen aus Goldnanoteilchen, Halbleiterquantenpunkten und Halbleiterstäbchen verwendet. Die so hergestellten Einheiten wurden mittels Elektronenstrahllithographie kontaktiert um ihre elektrische Leitwerte zwischen 4:2K und Raumtemperatur zu charakterisieren. Ein neues Konzept für die lösungsbasierte Herstellung von leitenden Goldnanodrähten mittels DNS-Templates wurde eingeführt: hierbei wurden DNS-Nanoformen eingesetzt in denen positionsspezifisch angedockte Goldkeime durch auÿenstromlose Goldabscheidung wachsen. Durch konfigurierbare Verbindungsstellen können sich die einzelnen Formen zu mikrometerlangen Strukturen verbinden. Während der folgendenden Abscheidung von Gold schränken die Wände der Gussformen über das Wachstum so ein, dass sehr homogene Nanodrähte gewonnen werden können. Goldnanodrähte wurden auch C-förmig hergestellt indem Goldnanoteilchen in der gewünschten Form auf DNS Origami-Nanoblättchen angeordnet und wiederum durch außenstromlose Goldabscheidung zu durchgängigen Drähten vergröbert wurden. Einige Abschnitte der DNS-Nanoform-geprägten Drähte zeigen metallische Leitfähigkeit, während andere durch Lücken zwischen den Goldkörnern deutlich höhere Widerstände aufweisen. Alle hergestellten C-förmigen Nanodrähte stellten sich als nicht-metallisch heraus, sie zeigten Eigenschaften von Hopping-, thermionischem und Tunneltransport in Abhängigkeit von der Temperatur. Die Anwesenheit dieser verschiedenen Transportmechanismen deutet darauf hin, dass die C-förmigen Nanodrähte aus metallischen Abschnitten bestehen welche aber nur schwach miteinander verbunden sind. Zwei verschiedene Metall/Halbleiter/Metall-Heterostrukturen wurden hergestellt: Metall/Halbleiternanstäbchen/Metall-Strukturen mittels DNS-Nanoformen und Metall/Quantenpunkt/Metall-Strukturen mittels DNS-Nanoröhren-Vorlagen Goldnanoteilchen konnten durch die DNA templates mit hoher Ausbeute neben den Halbleiterelementen platziert werden. Nach der erfolgter Anordnung wurden die Goldnanoteilchen gewachsen um durchgängige Heterostrukturen zu erhalten. Die Einflüsse des Inkubationsmediums und der -zeit, des Buffers, sowie der Quantenpunkt- und Goldnanopartikelkonzentrationen auf die Abscheidungseffzienz von Goldnanotailchen auf DNS Nanoröhren wurden systematisch untersucht. Zusätzlich zur Bestimmung der Morphologie der durch Selbstorganisation hergestellten Heterostrukturen, wurden auch ihre elektrischen Eigenschaften im Hinblick auf ihre Anwendung in nanelektronischen Bauelementen, wie Einzelelektronentransitoren untersucht.:1. Introduction 2. Overview on DNA Nanotechnology 2.1. Basic Concepts of DNA 2.1.1. Nanoscale Dimensions 2.2. Self-Assembled Architectures from DNA 2.3. DNA Origami: Nanomolds, Nanosheets and Nanotubes 2.3.1. DNA Origami Method 2.3.2. Nanomolds 2.3.3. Nanosheets 2.3.4. Nanotubes 2.4. DNA/DNA Origami-Templated Metallic Nanowire Fabrication 2.4.1. DNA/DNA Origami Templates 2.4.2. Metal Nanoparticle Attachment Yield 2.4.3. Metal Growth 2.5. Electron Transport Mechanisms of DNA-Templated Metallic Nanowires 2.5.1. Lithographically Defined Contacts and I-V Measurements of the DNA-Templated Metal Wires 2.5.2. Lithographically Defined Contacts and I-V Measurements of the DNA Origami-Templated Metal Nanowires 2.6. Applications 2.6.1. Introduction to Metamaterials: DNA-Templated Metamaterial Fabrication 2.6.2. Introduction to Single Electron Tunneling: A DNA-Templated Self-Assembly Concept 3. Experimental Details 3.1. Preparation of Substrates 3.2. DNA Origami Preparation and Deposition 3.2.1. DNA Nanomolds and Formation of linear mold superstructures 3.2.2. DNA Nanotubes 3.2.3. DNA Nanosheets 3.3. Metallization of DNA Origami Structures 3.3.1. DNA Nanomolds 3.3.2. DNA Nanotubes 3.3.3. DNA Nanosheets 3.3.4. Gold Growth on the DNA Origami Nanotube and Nanosheet 3.4. Semiconductor Nanoparticle Preparation and Assembly 3.4.1. CdS Semiconductor Quantum Rods for DNA Nanomold. 3.4.2. CdSe/ZnS Core-shell quantum Dots for DNA Nanotube 3.5. Deposition of DNA origami structures on SiO2 /Si surface 3.5.1. Deposition of DNA Nanomolds 3.5.2. Deposition of DNA Nanosheets and Nanotubes 3.6. Structural Characterization 3.6.1. Atomic Force Microscopy 3.6.2. Scanning Electron Microscopy 3.7. Electrical Characterization 4. Results and Discussion 4.1. DNA Nanomold-Templated Assembly of Conductive Gold Nanowires 4.1.1. Introduction 4.1.2. Results and Discussion 4.1.3. Conclusion 4.2. Conductance measurements on Gold/Semiconductor/Gold heterojunctions templated by DNA Nanomolds 4.2.1. Introduction 4.2.2. Results and Discussion 4.2.3. Conclusion 4.3. C-shaped Gold Nanowires Templated by DNA Nanosheet 4.3.1. Introduction 4.3.2. Results and Discussion 4.3.3. Conclusion 4.4. Self-Assembled Gold/Semiconductor/Gold heterojunctions templated by DNA Nanotube 4.4.1. Introduction 4.4.2. Results and Discussion 4.4.3. Conclusion 5. Conclusion and Future Work A. Supplement for DNA Nanomold-Templated Assembly of Conductive Gold Nanowires B. Conductance measurements on Gold/Semiconductor/Gold heterojunctions templated by DNA Nanomolds C. Supplement for C-shaped Gold Nanowires Templated by DNA Nanosheet D. Supplement for heterojunctions templated by DNA Nanotube / DNA allows self-assembly of nanoscale units into three dimensional nanostructures with definite shape and size in fields such as nanoelectronics, metamaterials and nanophotonics. Different DNA origami templates, such as: nanomold, nanosheet and nanotube templates have been used to assemble gold nanoparticles, quantum dots and semiconductor rods into nanowires and metal/semiconductor/metal heterostructures. Structures have been contacted using electron-beam lithography for electrical conductance characterization at temperatures between 4:2K and room temperature has been performed. A new concept has been introduced for the solution-based fabrication of gold nanowires. To this end, DNA nanomolds have been employed, inside which electroless gold deposition is initiated by site-specifically attached seeds. Using configurable interfaces, individual mold elements self-assemble into micrometer-long mold structures. During subsequent internal gold deposition, the mold walls constrain the metal growth, such that highly homogeneous nanowires are obtained. Gold nanowires have also been manufactured in a C-shape using gold nanoparticles arranged in the desired shape on a DNA origami nanosheet and enhanced to form a continuous wire through electroless gold deposition. Some sections of the DNA nanomold-templated wires show metallic conductance, while other sections of the wires have a much higher resistance which is caused by boundaries between gold grains. All C-shaped wires have been found to be resistive showing hopping, thermionic and tunneling transport characteristics at different temperatures. The different transport mechanisms indicate that the C-shaped nanowires consist of metallic segments which are weakly coupled along the wire. Two types of metal/semiconductor/metal heterostructures have been fabricated: Metal/semiconductor-rod/metal using DNA nanomolds and metal/quantum-dot/metal structures using DNA nanotube. AuNPs were assembled with high yield adjacent to the semiconductor material using origami templates. After the assembly, the gold nanoparticles were grown to produce continuous heterostructures. The influence of the incubation medium, time, buffer, quantum dot and gold nanoparticle concentration on nanoparticle attachment yield was systematically investigated for the nanotube templates. In addition to the determination of the self-assembled heterostructures' morphology, electrical properties were investigated to evaluate their applicability nanoelectronic devices such as single electron transistors.:1. Introduction 2. Overview on DNA Nanotechnology 2.1. Basic Concepts of DNA 2.1.1. Nanoscale Dimensions 2.2. Self-Assembled Architectures from DNA 2.3. DNA Origami: Nanomolds, Nanosheets and Nanotubes 2.3.1. DNA Origami Method 2.3.2. Nanomolds 2.3.3. Nanosheets 2.3.4. Nanotubes 2.4. DNA/DNA Origami-Templated Metallic Nanowire Fabrication 2.4.1. DNA/DNA Origami Templates 2.4.2. Metal Nanoparticle Attachment Yield 2.4.3. Metal Growth 2.5. Electron Transport Mechanisms of DNA-Templated Metallic Nanowires 2.5.1. Lithographically Defined Contacts and I-V Measurements of the DNA-Templated Metal Wires 2.5.2. Lithographically Defined Contacts and I-V Measurements of the DNA Origami-Templated Metal Nanowires 2.6. Applications 2.6.1. Introduction to Metamaterials: DNA-Templated Metamaterial Fabrication 2.6.2. Introduction to Single Electron Tunneling: A DNA-Templated Self-Assembly Concept 3. Experimental Details 3.1. Preparation of Substrates 3.2. DNA Origami Preparation and Deposition 3.2.1. DNA Nanomolds and Formation of linear mold superstructures 3.2.2. DNA Nanotubes 3.2.3. DNA Nanosheets 3.3. Metallization of DNA Origami Structures 3.3.1. DNA Nanomolds 3.3.2. DNA Nanotubes 3.3.3. DNA Nanosheets 3.3.4. Gold Growth on the DNA Origami Nanotube and Nanosheet 3.4. Semiconductor Nanoparticle Preparation and Assembly 3.4.1. CdS Semiconductor Quantum Rods for DNA Nanomold. 3.4.2. CdSe/ZnS Core-shell quantum Dots for DNA Nanotube 3.5. Deposition of DNA origami structures on SiO2 /Si surface 3.5.1. Deposition of DNA Nanomolds 3.5.2. Deposition of DNA Nanosheets and Nanotubes 3.6. Structural Characterization 3.6.1. Atomic Force Microscopy 3.6.2. Scanning Electron Microscopy 3.7. Electrical Characterization 4. Results and Discussion 4.1. DNA Nanomold-Templated Assembly of Conductive Gold Nanowires 4.1.1. Introduction 4.1.2. Results and Discussion 4.1.3. Conclusion 4.2. Conductance measurements on Gold/Semiconductor/Gold heterojunctions templated by DNA Nanomolds 4.2.1. Introduction 4.2.2. Results and Discussion 4.2.3. Conclusion 4.3. C-shaped Gold Nanowires Templated by DNA Nanosheet 4.3.1. Introduction 4.3.2. Results and Discussion 4.3.3. Conclusion 4.4. Self-Assembled Gold/Semiconductor/Gold heterojunctions templated by DNA Nanotube 4.4.1. Introduction 4.4.2. Results and Discussion 4.4.3. Conclusion 5. Conclusion and Future Work A. Supplement for DNA Nanomold-Templated Assembly of Conductive Gold Nanowires B. Conductance measurements on Gold/Semiconductor/Gold heterojunctions templated by DNA Nanomolds C. Supplement for C-shaped Gold Nanowires Templated by DNA Nanosheet D. Supplement for heterojunctions templated by DNA Nanotube

DNA Nanotechnology allows the synthesis of nanometer sized objects that can be site specifically functionalized with a large variety of materials. However, many DNA structures need a higher ionic strength than that in common cell culture buffers or in bodily fluids to maintain their integrity and can be degraded quickly by nucleases. The aim of this dissertation was to overcome this deficiency with the help of cationic PEG-poly-lysine block copolymers that can electrostatically cover the DNA nanostructures to form “DNA origami polyplex micelles” (DOPMs). This straightforward, cost-effective and robust route to protect DNA-based structures could therefore enable applications in biology and nanomedicine, where un-protected DNA origami would be degraded. Moreover, owing to high polarity, the DNA-based structures are restricted to the aque-ous solution based buffers only. Any attempt to change the favorable conditions, leads to the distortion of the structures. In this work it was demonstrated that, by using the polyplex micellization strategy, the organic solubility of DNA origami structures can be improved. The strategy was also extended to functional ligands that are otherwise not soluble in organic solvents. With this strategy, it is now also possible to perform organic solution reactions on the DNA-based structures, opening up the possibility to use hydro-phobic organic reagents to synthesize novel materials. The polyplex micellization strategy therefore presents a cheap, robust, modular, reversible and versatile method to not only solubilize DNA structures in organic solvents but also improve their stability in biological environments. A third project was based on the possibility to synthesize complementary sequences to single-stranded gap regions in the DNA origami scaffold cost-effectively by a DNA polymerase rather than by a DNA synthesizer. For this purpose, four different wireframe DNA origami structures were designed to have single-stranded gap regions. The introduction of flexible gap regions resulted in fully collapsed or partially bent structures due to entropic spring effects. These structures were also used to demonstrate structural transformations with the help of DNA polymerases, expanding the collapsed bent structures to straightened tubes. This approach presents a powerful tool to build DNA wireframe structures more material-efficiently, and to quickly prototype and test new wireframe designs that can be expanded, rigidified or mechanically switched.:Abstract v Publications vii Acknowledgements ix Contents xiii Chapter 1 Introduction 1 1.1 Nanotechnology 1 1.1.1 History of nanotechnology 1 1.1.2 Phenomena that occur at nanoscale 4 1.1.3 Nature’s perspective of nanotechnology 4 1.1.4 Manufacturing nanomaterials 6 1.2 Deoxyribonucleic acid (DNA) 8 1.2.1 DNA, the genetic material, “The secret of life” 8 1.2.2 Structure of DNA 9 1.2.3 DNA synthesis 15 1.2.4 Stability of DNA 18 1.3 DNA nanotechnology 20 1.3.1 Historical development 20 1.3.2 DNA tile motifs 21 1.3.3 Directed nucleation assembly and algorithmic assembly 23 1.3.4 Scaffolded DNA origami and single-stranded DNA tiles 25 1.3.5 Expanding the design space offered by DNA 27 1.3.6 Assembling heterogeneous materials with DNA 30 1.3.7 Functional devices built using DNA nanostructures 35 Chapter 2 Motivation and objectives 40 Chapter 3 Block copolymer micellization as a protection strategy for DNA origami 42 3.1 Introduction 42 3.1.1 Cellular delivery of DNA nanostructures 42 3.1.2 The need for stability of DNA nanostructures 43 3.1.3 Non-viral gene therapy 44 3.2 Results and discussions 46 3.2.1 Strategy to form DNA origami polyplex micelles (DOPMs) 46 3.2.2 Optimizations 46 3.2.3 Decomplexation 53 3.2.4 Stability tests 55 3.2.5 Short PEG-PLys block copolymer 58 3.2.6 Compatibility with bulky ligands 59 3.2.7 Accessibility of handles on DOPMs 63 3.3 Conclusion 64 3.4 Outlook and state of the art 65 3.5 Methods 67 3.5.1 DNA origami folding 67 3.5.2 Preparation of ssDNA functionalized AuNPs 68 3.5.3 Agarose gel electrophoresis 69 3.5.4 Block copolymer preparation 70 3.5.5 DNA origami polyplex micelle preparation 70 3.5.6 Decomplexation of DOPM using dextran sulfate 73 3.5.7 Stability tests 74 3.5.8 tSEM characterization 75 3.5.9 AFM imaging 76 Chapter 4 Improving organic solubility and stability of DNA origami using polyplex micellization 77 4.1 Introduction 77 4.2 Results and discussions 79 4.2.1 Strategy for organic solubility of DNA origami 79 4.2.2 Proof of concept using AuNPs functionalized with ssDNA 80 4.2.3 Extending the strategy to DNA origami 82 4.2.4 Optimizations 86 4.2.5 Compatibility with functional ligands 88 4.2.6 Functionalization of DNA origami in organic solvent 94 4.3 Conclusion and outlook 95 4.4 Methods 97 4.4.1 Conjugation of functional ligands to DNA origami 97 4.4.2 Organic solubility 98 4.4.3 Reactions in organic solution on DOPMs 99 4.4.4 Fluorescence imaging using gel scanner 100 Chapter 5 Structural transformation of wireframe DNA origami via DNA polymerase assisted gap-filling 101 5.1 Introduction 101 5.2 Results and discussion 102 5.2.1 Design of the structures 102 5.2.2 Folding of gap-structures 105 5.2.3 Single-stranded DNA binding proteins 107 5.2.4 Gap filling with different polymerases 109 5.2.5 Gap filling with Phusion high-fidelity DNA polymerase 111 5.2.6 Optimization of the extension reaction using T4 DNA polymerase 115 5.2.7 Secondary structures 121 5.2.8 Folding kinetics of gap origami 124 5.2.9 Bending of tubes 125 5.3 Conclusion 126 5.4 Outlook 127 5.5 Methods 128 5.5.1 DNA origami folding 128 5.5.2 Gap filling of the wireframe DNA origami structures 128 5.5.3 Agarose gel electrophoresis 130 5.5.4 PAGE gel analysis 130 5.5.5 tSEM characterization 131 5.5.6 AFM imaging 131 5.5.7 AGE based folding-yield estimation 132 5.5.8 Gibbs free energy simulation using mfold 132 5.5.9 Staple list for folding the DNA origami triangulated structures 132 Appendix 134 A.1 Additional figures from chapter 3 134 A.2 Additional figures from chapter 4 137 A.3 Additional figures from chapter 5 149 Bibliography 155 Erklärung 171

Die rasant fortschreitende Digitalisierung in allen Bereichen des täglichen Lebens erzeugt einen immensen Bedarf an Mikrochips. Um diese Mikrochips zu erzeugen, durchlaufen Wafer eine Vielzahl an ressourcenaufwändigen Prozessschritten. In dieser Arbeit werden zwei Ansätze der reversiblen Immobilisierung von Biopolymeren verfolgt, welche als Grundlage für eine alternative Herstellung von Mikrochips fungieren könnten. Zum einen sollte die reversible Immobilisierung von Polyplexen auf Basis von 6HB DNA-Origami und kationischen Polymethacrylaten bzw. Poly(2-oxazolin)en auf strukturierten PDMAEMA-Bürsten nach Nawroth et al.[1],[2] realisiert werden. Zum anderen war die reversible Immobilisierung von Mikrotubuli nach Ionov et al.[3] unter Verwendung eines grafting-from Systems Ziel dieser Arbeit. Für die Immobilisierung von Polyplexen auf Polymerbürsten wurden die Polymere so gestaltet, dass sie mehrere Funktionen erfüllen können. Die Copolymere verfügten über einen kationischen Bereich mit 20 Wiederholeinheiten, welcher zur Anbindung an die 6HB diente, einen hydrophilen Spacer sowie mehrere Funktionalisierungsstellen. Das zuerst untersuchte methacrylische System auf Basis von HEMA stellte sich für den hier vorgesehenen Verwendungszweck als ungeeignet heraus. Für das Poly(2-oxazolin)-System wurden die nach Cesana et al.[4] und Hartlieb et al.[5] synthetisierten aminfunktionalisierten Monomere AmProOx, AmBuOx, AmPentOx und AmDecOx, sowie das nach He et al.[6] synthetisierte MAMeOx verwendet. Die Synthese der verwendeten Monomere konnte optimiert bzw. AmProOx und AmDecOx im Zuge dieser Arbeit erstmalig synthetisiert werden. AmProOx, AmBuOx und AmPentOx konnten mittels LCROP erstmalig in dieser Arbeit mit Benzyltosylat direkt initiiert und homopolymerisiert werden. Diese AmOx-Derivate erlauben als Blockcopolymer mit MeOx eine effektive und gut kontrollierbare Polyplexbildung mit 6HB. Dies wurde mittels GEP und AFM verifiziert. Es zeigte sich, dass MAMeOx ungeeignet war, da es keinen Polyplex mit 6HB bildete. Als mögliche Ursachen sind der zu geringe Abstand zwischen den Aminen in der Seitenkette sowie der zu geringe Abstand zwischen dem Amin in der Seitenkette und dem Rückgrat zu nennen. Die AmOx konnten in einem Triblockcopolymer zusammen mit MeOx bzw. EtOx und PynOx copolymerisiert werden. Über den PynOx-Block ist eine Funktionalisierung mittels Click Chemie möglich.[7] Es konnte gezeigt werden, dass die Polyplexe in ihrer Peripherie vollumfänglich durch die Polymere zusätzlich funktionalisiert werden können. So wurden Fluoreszenzfarbstoffe ebenso wie CRP Initiatoren eingeführt. Die angeschlossene Pfropfungspolymerisation auf Basis der CuCRP zeigte, im Vergleich zur Literatur,8 einen bis zum Zeitpunkt der Erstellung dieser Arbeit unbekannten Zuwachs an Polymerhülle von bis zu 70 nm (240%) innerhalb von 15 min. Eine Immobilisierung der 6HB-POx Polyplexe nach Nawroth et al.[1],[2] auf PDMAEMA-Bürsten zeigte sich als nicht erfolgreich. Es konnte auf den nanostrukturierten PDMAEMA-Bürsten keine Abscheidung der Polyplexe beobachtet werden. In Zukunft könnten die entwickelten Polyplexe durch eine Funktionalisierung der PynOx Einheiten mit Metallisierungskeimen als Nanodrahttemplate verwendet werden. Außerdem ist die Nutzung der POx-DNA-Origami- Polyplexe als formstabile Wirkstofftransportsysteme denkbar, ebenso wie die Verwendung der kationischen POx als Ersatz für Polyethylenimin (PEI)[9]–[11] in der Gentherapie. Für die Immobilisierung von Mikrotubuli Gleit-Assays mit gepfropften PNIPAM-Bürsten auf Grundlage der Publikation von Ionov et al.[3] wurde erstmalig die SI-CuCRP mit PNIPAM auf Gold untersucht. Hierfür wurde zuerst eine geeignete Initiator-SAM ermittelt, wobei die Wahl auf BiB UD-SH fiel, welche eine Schichtdicke von ca. 1,2 nm bei einem Wasserkontaktwinkel von ca. 72° aufwies. Um die ideale PNIPAM Schichtdicke für die Immobilisierung zu ermitteln, wurden mehrere Gradienten mit unterschiedlichen Schichtdicken hergestellt. Als ideale Schichtdicke erwies sich eine Höhe von 10 bis 20 nm PNIPAM im kollabierten Zustand. Die generierten PNIPAM Schichten auf Gold zeigten in den Gleit-Assays ein effektives Verhalten bezüglich der Immobilisierung der Mikrotubuli. Dies zeigte sich dadurch, dass im gequollenen Zustand unterhalb der LCST der PNIPAM-Bürsten die Mikrotubuli von der Oberfläche gelöst wurden. Oberhalb der LCST konnten sich die Mikrotubuli frei auf der Oberfläche bewegen. Gleiches konnte auf im Anschluss präparierten UV-strukturierten Proben nachvollzogen werden. Die Mikrotubuli waren nicht in der Lage, sich unterhalb der LCST auf den PNIPAM-Strukturen zu bewegen, jedoch auf Bereichen ohne PNIPAM. Oberhalb der LCST war es den Mikrotubuli wieder möglich, sich frei zu bewegen. Das entwickelte System für die reversible Immobilisierung von Mikrotubuli könnte im nächsten Schritt auf die von Nicolau et al.[12] entwickelten Arrays übertragen werden. [1] Nawroth, J. F.; Neisser, C.; Erbe, A.; Jordan, R. Nanoscale 2016, 8 (14), 7513–7522. [2] Nawroth, J. F. Synthese nanostrukturierter Polymerbürsten zur reversiblen Immobilisierung von DNA Origami, Dissertation, TU Dresden, 2017. [3] Ionov, L.; Stamm, M.; Diez, S. Nano Lett. 2006, 6 (9), 1982–1987. [4] Cesana, S.; Auernheimer, J.; Jordan, R.; Kessler, H.; Nuyken, O. Macromol. Chem. Phys. 2006, 207 (2), 183–192. [5] Hartlieb, M.; Pretzel, D.; Kempe, K.; Fritzsche, C.; Paulus, R. M.; Gottschaldt, M.; Schubert, U. S. Soft Matter 2013, 9 (18), 4693–4704. [6] He, Z.; Miao, L.; Jordan, R.; S-Manickam, D.; Luxenhofer, R.; Kabanov, A. V. Macromol. Biosci. 2015, 15 (7), 1004–1020. [7] Luxenhofer, R.; Jordan, R. Macromolecules 2006, 39 (10), 3509–3516. [8] Tokura, Y.; Jiang, Y.; Welle, A.; Stenzel, M. H.; Krzemien, K. M.; Michaelis, J.; Berger, R.; Barner-Kowollik, C.; Wu, Y.; Weil, T. Angew. Chemie - Int. Ed. 2016, 55 (19), 5692–5697. 9Boussif, O.; LezoualC’H, F.; Zanta, M. A.; Mergny, M. D.; Scherman, D.; Demeneix, B.; Behr, J. P. Proc. Natl. Acad. Sci. U. S. A. 1995, 92 (16), 7297–7301. [10] Horbinski, C.; Stachowiak, M. K.; Higgins, D.; Finnegan, S. G. BMC Neurosci. 2001, 2. [11] Dodds, E.; Piper, T. A.; Murphy, S. J.; Dickson, G. J. Neurochem. 1999, 72 (5), 2105–2112. [12] Nicolau, D. V; Lard, M.; Korten, T.; van Delft, F. C. M. J. M.; Persson, M.; Bengtsson, E.; Månsson, A.; Diez, S.; Linke, H.; Nicolau, D. V. Proc. Natl. Acad. Sci. 2016, 113 (10), 2591–2596.:Inhaltsverzeichnis Danksagung I Abkürzungsverzeichnis III 1. Einleitung 1 2. Grundlagen 3 2.1. DNA-Origami 3 2.2. Mikrotubuli als Nanoroboter 9 2.3. Poly(2-oxazolin)e 14 2.3.1. 2-Substituierte 2-Oxazoline 14 2.3.2. Lebende kationische ringöffnende Polymerisation 15 2.4. Oberflächenmodifikation mit Polymerbürsten 18 2.4.1. Selbstorganisierende Monoschichten 18 2.4.2. Polymerbürsten 19 2.4.3. Pfropfungspolymerisatonsarten 22 2.4.5. Thermoresponsive Polymerbürsten 27 2.5. Rasterkraftmikroskopie 29 3. Motivation 31 4. Ergebnisse und Diskussion 33 4.1. Kationische Bürsten auf Polymethacrylatbasis 33 4.1.1. Synthese der kationischen Copolymere 34 4.1.2. Funktionalisierung mit BiBB 38 4.1.3. Pfropfungspolymerisation 40 4.1.4. Polyplexbildung mit kationischen Polymethacrylaten 43 4.2. Synthese von 2-Oxazolinen mit Aminofunktionalität 45 4.3. Kationische Poly(2-oxazolin)e zur DNA-Origami-Komplexierung 50 4.3.1. Kinetik der Homopolymerisation der AmOx-Monomere 50 4.3.2. Erprobung der Click-Reaktion und der Pfropfung 53 4.3.3. Poly(2-oxazolin)e mit Aminfunktionalität zur Stabilisierung von DNA-Origami 59 4.3.4. Poly(2-oxazolin)e mit BiB-Typ Endfunktionalisierung 65 4.3.5. Kationische Poly(2-oxazolin)e für die Funktionalisierung von Polyplexen 69 4.3.6. Pfropfungspolymerisation auf immobilisierten Polyplexen 72 4.3.7. Pfropfungspolymerisation auf DNA-Origami in Lösung 74 4.3.8. Polyplex-gestütztes, gerichtetes Assemblieren von DNA-Origami 77 4.4. Reversible Immobilisierung von Mikrotubuli durch Polymerbürsten 81 4.4.1. Oberflächenmodifizierung 81 4.4.2. Mikrotubuli-Gleit-Assays auf PNIPAM-Gradienten 84 4.4.3. Mikrotubuli-Gleit-Assays auf strukturierten PNIPAM-Bürsten 89 5. Zusammenfassung und Ausblick 92 6. Experimentalteil 96 6.1. Geräte 96 6.2. Verbrauchsgüter 99 6.2.1. Chemikalien 99 6.2.2. 6-Helixbündel 6HB 99 6.2.3. Wafer 99 6.3. Synthese der Initiatoren 100 6.3.1. Benzyltosylat BnOTs 100 7.3.2. 2-Azidoethyl-2-bromoisobutyrat 100 6.4. Synthese der Monomere 103 6.4.1. Allgemeine Synthesevorschrift der AmOx Monomere 103 6.4.2. 2-(N-Methyl)aminomethyl-2-oxazolin MAMeOx 106 6.5. Polymersynthese 108 6.5.2. Homopolymerisation der AmOx Monomere 113 6.5.3. P(MeOx-grad-PynOx) P1 115 6.5.4. P(MAMeOx-co-MeOx-grad-PynOx) P2Boc 115 6.5.5. Synthese der P(AmOx-co-MeOx) 116 6.5.6. Allgemeine Synthesevorschrift der LCROP mit BiB-OH Terminierung 118 6.5.7. Allgemeine Synthesevorschrift der CuCRP mit einem POx-Makroinitiator 122 6.5.8. P(AmProOx-co-MeOx-grad-PynOx) P6Boc 126 6.5.10. P(AmPentOx-co-EtOx-grad-PynOx) P8Boc 128 6.6. Polymeranaloge Funktionalisierung 129 6.6.1. Allgemeine Bedingung der Funktionalisierung der HEMA-Gruppen mit BiBB 129 6.6.2. Pfropfungspolymerisation an PMETAC10-BiB mit HEMA 132 6.6.3. Allgemeine Vorschrift der Abspaltung der Boc-Schutzgruppe 133 6.6.4. Allgemeine Bedingungen der Huisgen 1,3-dipolaren kupferkatalysierten Azid-Alkin-Cycloaddition 137 6.6.5. Pfropfungspolymerisation an P1-BiB 141 6.6.6. Pfropfungspolymerisation an P2Boc-BiB 142 6.7. DNA-Origami-Polyplexe 143 6.7.1. Polyplexbildung 143 6.7.2. Präparation für AFM-Messung 143 6.7.3. Oberflächenbasierte Pfropfungspolymerisation 144 6.7.4. Lösungsbasierte Pfropfungspolymerisation 144 6.7.5. Polyplexabscheidung auf nanostrukturierten PDMAEMA Bürsten 144 6.8. Oberflächenmodifikation 145 6.8.1. Präparation der SiO2-Wafer mit PF-SAM 145 6.8.2. Selbst-initiierende Photopfropfung und Photopolymerisation SIPGP 145 6.8.3. Präparation Au-Wafer mit Thiol-SAM 145 6.8.4. UV-Lithografie 146 6.8.5. SI-CuCRP 146 6.9. Gleit-Assays 147 7. Quellen 149

DNA nanotechnology has developed a versatile set of methods to utilise DNA self-assembly for the bottom-up construction of arbitrary two- and three-dimensional DNA objects in the nanometre size range, and to functionalise the structures with unprecedented site-specificity with nanoscale objects such as metallic and semiconductor nanoparticles, proteins, fluorescent dyes, or synthetic polymers. The advances in structure assembly have resulted in the application of functional DNA-based nanostructures in a gamut of fields from nanoelectronic circuitry, nanophotonics, sensing, drug delivery, to the use as host structure or calibration standard for different types of microscopy. However, the analytical means for characterising DNA-based nanostructures drag behind these advances. Open questions remain, amongst others in quantitative single-structure evaluation. While techniques such as atomic force microscopy (AFM) or transmission electron microscopy (TEM) offer feature resolution in the range of few nanometres, the number of evaluated structures is often limited by the time-consuming manual data analysis. This thesis has introduced two new approaches to quantitative structure evaluation using AFM and super-resolution fluorescence microscopy (SRM). To obtain quantitative data, semi-automated computational image analysis routines were tailored in both approaches. AFM was used to quantify the attachment yield and placement accuracy of poly(3-tri(ethylene glycol)thiophene)-b-oligodeoxynucleotide diblock copolymers on a rectangular DNA origami. This work has also introduced the first hybrid of DNA origami and a conjugated polymer that uses a highly defined polythiophene derivative synthesised via state-of-the-art Kumada catalyst-transfer polycondensation. Among the AFM-based studies on polymer-origami-hybrids, this was the first to attempt near-single molecule resolution, and the first to introduce computational image analysis. Using the FindFoci tool of the software ImageJ revealed attachment yields per handle between 26 - 33%, and determined a single block copolymer position with a precision of 80 - 90%. The analysis has pointed out parameters that potentially influence the attachment yield such as the handle density and already attached objects. Furthermore, it has suggested interactions between the attached polymer molecules. The multicolour SRM approach used the principles of single-molecule high-resolution co-localisation (SHREC) to evaluate the structural integrity and the deposition side of the DNA origami frame “tPad” based on target distances and angles in a chiral fluorophore pattern the tPads were labelled with. The computatinal routine that was developed for image analysis utilised clustering to identify the patterns in a sample’s signals and to determine their characteristic distances and angles for hundreds of tPads simultaneously. The method excluded noise robustly, and depicted the moderate proportion of intact tPads in the samples correctly. With a registration error in the range of 10 -15 nm after mapping of the colour channels, the precision of a single distance measurements on the origami appeared in the range of 20 - 30 nm. By broadening the scope of computational AFM image analysis and taking on a new SRM approach for structure analysis, this work has presented working approaches towards new tools for quantitative analysis in DNA nanotechnology. Furthermore, the work has presented a new approach to constructing hybrid structures from DNA origami and conjugated polymers, which will open up new possibilities in the construction of nanoelectronic and nanophotonic structures.

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Der in den Ingenieurwissenschaften zunehmend populäre Einsatz der Origami-Technik eröffnet neue Möglichkeiten zur Herstellung von effizienten Tragkonstruktionen [1]–[5]. In Verbindung mit leistungsfähigen, zementbasierten Verbundwerkstoffen bietet die Origami-Technik einen innovativen Ansatz für Entwurf und Realisierung von leichten tragenden Strukturen nach dem Prinzip form follows force – dem Grundgedanken des SPP 1542. [Aus: Motivation und Zielsetzung] / The increasingly popular use of origami technology in the engineering sciences opens up new possibilities for the manufacture of efficient load-bearing structures [1]–[5]. In combination with high-performance, cement-based composite materials, origami technology of ers an innovative approach to the design and realisation of lightweight load-bearing structures based on the principle form follows force –the basic idea of SPP 1542. [Off: Motivation and objectives]

This research details an investigation into processes employing laser technology to create design-led three-dimensional textiles. An analysis of historical and contemporary methods for making three-dimensional textiles categorises these as processes that construct a three-dimensional textile, processes that apply or remove material from an existing textile to generate three-dimensionality or processes that form an existing textile into a three-dimensional shape. Techniques used in these processes are a combination of joining, cutting, forming or embellishment. Laser processing is embedded in textile manufacturing for cutting and marking. This research develops three novel processes: laser-assisted template pleating which offers full design freedom and may be applied to both textile and non-textile materials. The language of origami is used to describe designs and inspire new design. laser pre-processing of cashmere cloth which facilitates surface patterning through laser interventions in the manufacturing cycle. laser sintering on textile substrates which applies additive manufacturing techniques to textiles for the generation of three-dimensional surface patterning and structures. A method is developed for determining optimum parameters for laser processing materials. It may be used by designers for parameter selection for processing new materials or parameter modification when working across systems.

This dissertation includes two parts. In the first part the study is focused on the fabrication of multifunctional thin films for photovoltaic applications. There is no doubt about the importance of transforming world reliance from traditional energy resources, mainly fossil fuel, into renewable energies. Photovoltaic section still owns very small portion of the production, despite its fast growth and vast research investments. New methods and concepts are proposed in order to improve the efficiency of traditional solar cells or introduce new platforms. Recently, ferroelectric photovoltaics have gained interest among researchers. First objective in application of ferroelectric material is to utilize its large electric field as a replacement for or improvement of built-in electric field in semiconductor p-n junctions which is responsible for the separation of generated electron-hole pairs. Increase in built in electric field will increase open-circuit voltage of the solar cell. In this regard, thin films of ferroelectric hafnium dioxide doped with silicon have been fabricated using physical vapor deposition techniques. Scanning probe microscopy techniques (PFM and KPFM) have been employed to analyze ferroelectric response and surface potential of the sample. The effects of poling direction of the ferroelectric film on the surface potential and current-voltage characteristics of the cell have been investigated. The results showed that the direction of poling affects photoresponse of the cell and based on the direction it can either improved or diminished. In the second part of this work, epitaxial thin films have been synthesized with physical vapor deposition techniques such as sputtering and electron beam evaporation for the ultimate goal of producing multifunctional three-dimensional structures. Three-dimensional structures have been used for applications such as magnetic sensors, filters, micro-robots and can be used for modification of the surface of solar cells in order to improve light absorption and efficiency. One of the important techniques for producing 3-D structures is using origami techniques. The effectiveness of this technique depends on the control of parameters which define direction of bending and rolling of the film or curvature of the structure based on the residual stress in the structure after film’s release and on the quality and uniformity of the film. In epitaxially grown films, the magnitude and direction of the stress are optimized, so the control over direction of rolling or bending of the film can be controlled more accurately. For this purpose, deposition conditions for epitaxy of Zn, Fe, Ru, Ti, NaCl and Cr on Si, Al2O3 or MgO substrates have been investigated and optimized. Crystallinity, composition and morphology of the films were characterized using reflective high energy diffraction (RHEED), Auger electron spectroscopy (AES), energy dispersive X-ray (EDX), and scanning electron microscopy (SEM).

<div>Thin walled structures find wide applications in automotive industry as energy absorption devices. A great deal of research has been conducted to design thin walled structures, where the main objective is to reduce peak crushing forces and increase energy absorption capacity. With the advancement of computers and mathematics, it has been possible to develop 2D patterns which when folded turn into complex 3D structures. This technology can be used to develop patterns for getting structures with desired properties. </div><div>In this study, square origami tubes with folding pattern (Yoshimura pattern) is designed and studied extensively using numerical analysis. An accurate Finite Element Model (FEM) is developed to conduct the numerical analysis. A parametric study was conducted to study the influence of geometric parameters on the mechanical properties like peak crushing force, mean crushing force, load uniformity and maximum intrusion, when subjected to dynamic loading. </div><div>The results from this analysis are studied and various conclusions are drawn. It is found that, when the tube is folded with the pattern having specific dimension, the performance is enhanced significantly, with predictable and stable collapse. It is also found that the stiffness of the module varies with geometrical parameters. With a proper study it is possible to develop origami structures with functionally graded stiffness, the performance of which can be tuned as per requirement, hence, showing promising capabilities as an energy absorption device where progressive collapse from near to end impact end is desired.</div><div><br></div>

Orientador(a): Rosangela da Silva Leote / Banca: José Spaniol / Banca: Almir Almas / Resumo: Nesta dissertação, eu discuto algumas das relações entre arte, ciência e tecnologia a partir da conexão entre o papel e a eletrônica. O foco da dissertação é como o papel, tecnologia ancestral, pode ganhar outras aplicações, especialmente na arte, ao se conectar a sistemas de código aberto para robótica. O conhecimento teórico e experimental sobre o tema converte-se na produção de uma série de objetos cinéticos apresentados em uma exposição junto com o texto final desta dissertação, que contém também um tutorial abrindo o código das obras realizadas, permitindo a sua replicação por outras pessoas / Abstract: In this text, I discuss relations between art, science and technology starting from the connection between paper and electronics. The focus is how paper, ancestral technology, can gain other applications, especially in art, when connecting to open source systems for robotics. Theoretical and experimental knowledge on the subject compose a series of kinetic objects presented in an exposition along with the final text of this dissertation, which also contains a tutorial opening the code of the works carried out, allowing their replication by other people / Mestre