ABSTRACT Classifying and Mapping Diversity in a Species-Poor System: the mangrove meta-community of Laguna Chacahua National Park, Oaxaca, Mexico by Elizabeth Kay Weisgerber Both field transects and imagery grid plots were analyzed with the goal of creating a community classification map for the mangrove forest of Parque Nacional Lagunas de Chacahua. In total, data was collected in 49 sites throughout the park, recording measures such as DBH, basal area, estimated dominance, frequency, cover and relative dominance. Field locations were marked and georeferenced with a GPS and grid plots overlaid on satellite imagery of the park were generated via a random number table. Species' attributes delineating the identifiable features for each dominant species within Chacahua were noted and used to recognize patterns in species assemblages. Fourteen distinct ‘sub-communities’ within the mangrove meta-community were recognized using this data and verified with 165 field photos. Relative dominance values were compared between field and grid data. These data revealed a similar pattern with Rhizophora mangle being most dominant under each method. Avicennia germinans was second in relative dominance, Laguncularia racemosa a close third while Conocarpus erectus was rarely found, most likely due to over-harvesting. Rapid degradation of mangrove forests is occurring on a global scale. Understanding the complex dynamics that occur within the mangrove meta-community is essential to its conservation. Vegetation maps are essential tool in monitoring changes throughout the mangrove but are rarely of sufficient detail for everyday use. Generating highly detailed vegetation maps in a cost-effective and timely manner is an important step for their conservation, particularly in developing countries. This study demonstrates methods and techniques for producing a vegetation map that portrays the level of complexity that exists within the species-poor mangrove environment. This map will be donated to the management team of Parque Nacional Lagunas de Chacahua to better aid in management.

Knowledge of the impacts of white-tailed deer (Odocoileus virginianus; hereafter deer) as dominant herbivores throughout the Southeastern United States of America is lacking. To address this, three paired experimental units of exclosures and controls were constructed in 2000 on three Wildlife Management Areas across Mississippi within the ecoregions of the Upper Coastal Plain, Lower Coastal Plain, and the Mississippi Alluvial Valley. Vegetation was sampled in the summers of 2000, 2005, and 2021 including vegetation structure, canopy coverage, basal area, and species composition. Additionally, in 2005 and 2021, biomass was sampled to determine potential impacts on nutritional carrying capacity. Among the three study sites, vegetation metrics followed successional trends and were not influenced by herbivory or lack thereof. Additionally, regional differences in nutritional carrying capacity seemed to be of greater importance than herbivory. This research illustrates the difference in effects of deer by region and forest type.

Utilizing biomass such as lignin, bamboo, soybean, corn stalk, rice husk, etc., as a carbon source to produce graphene-based nanomaterials has been reported recently. However, the potential of using such nanomaterials for engineering and environmental applications has not been realized. This dissertation investigates the use of graphene-based nanomaterials synthesized from using biomass as a carbon source for water remediation and cement-based composites’ (CBCs) property enhancement. The first chapter introduces graphene and graphene-based nanomaterials, as well as the synthesis and application of graphene-based nanomaterials for removing heavy metals in an aqueous solution and for property enhancement in CBCs. The experimental investigation on the pyrolytic synthesis of graphene-encapsulated iron nanoparticles from biochar (BC) as the carbon source (BC-G@Fe0) was covered in the second chapter. Two synthetic routes for producing BC-G@Fe0, i.e., impregnation-carbonization (route-I) and pyrolysis-impregnation-carbonization (route-II) processes, were investigated experimentally using different characterization techniques and heavy metal removal methods. The third chapter reports the experimental performances of the heavy metal removal of Pb2+, Cu2+, and Ag+ from an aqueous solution using BC-G@Fe0. The effectivenesses of various adsorption benchmarks, such as pH, kinetics, and isotherms were assessed. Additionally, the removal efficiency of BC-G@Fe0 was evaluated. BC-G@Fe0 sample made from route II, in particular, FeCl2-impregnated-BC with 15% wt% iron loading carbonized at 1000 ℃ for 1h showed promising Pb2+, Cu2+, and Ag+ removal capacities of 0.30, 1.58, and 1.91 mmol/g, respectively. The fourth chapter experimentally investigated the reinforcement effect of commercially sourced, industrial graphene nanoplates (IG) on the mechanical properties of CBCs. This investigation was based on a hypothesis that the uniform dispersion of IG would significantly enhance the compressive strength of CBC. The main outcome of this research was that, while the wet dispersion mixing process of IG into CBC did not consistently yield significant increases in the composite compressive strength, but the newly proposed dry dispersion process demonstrated significant increases (22%) in the composite compressive strength. Chapter Five investigated the synthesis of lignin-based graphene nanoplatelets (LG) and their application in CBC reinforcement. The main findings were that LG did not show impressive increases compared to IG, even when dry dispersion was introduced. This was attributed to LG's lack of effective surface area compared to IG. Finally, a general conclusion and outlook for the future of research into biomass-based graphene nanomaterials were discussed in chapter six.

Piratebush (Buckleya distichophylla (Nutt.) Torr.) is a rare, hemiparasitic shrub with the only extant populations in western North Carolina, northeastern Tennessee, and southwestern Virginia. The preferred natural hosts of piratebush, Carolina and eastern hemlocks, have seen sharp declines over the last decade due to the invasive hemlock woolly adelgid. Virginia pine, another important host of piratebush, is also susceptible to disease, specifically Cronartium appalachianum, a rust fungus for which piratebush is the secondary host. This study described and analyzed current demographic parameters of three Tennessee piratebush populations. Additionally, spatial patterns of disease and demographic characters were analyzed. These data were compared to data from previous censuses to infer the impacts of diseases on piratebush and its host. All three populations were relatively stable in numbers and age structure over the past thirty years. Plant height and stems per shrub were similar among populations and stable over time. Seedlings represented 14%-19% of populations and non-flowering plants 33%-41% of populations. Two populations had an equal sex ratio and one population was male-biased. Disease prevalence was similar among populations but disease was more severe at Temple Ridge. The effects of hemlock decline were most acute at the Temple Ridge population where areas of high hemlock decline were associated with lower vigor piratebush individuals. Piratebush individuals near Virginia pines were more likely to be infected by C. appalachianum, and individuals infected by the rust fungus were more likely to have lower vigor. If hemlock decline is causing a piratebush host shift toward Virginia pine, piratebush populations may also decline because of potential enhanced infection by C. appalachianum. Treatment to prevent HWA infestation may be needed because of its effectiveness in improving the health of both hemlock and piratebush populations.

Species of trees inhabit diverse and heterogeneous environments, and often play important ecological roles in such communities. As a result of their vast ecological breadth, trees have become adapted to various environmental pressures. In this dissertation I examine various environmental factors that drive evolutionary dynamics in threePinusspecies in California and Nevada, USA. In chapter two, I assess the role of management influence of thinning, fire, and their interaction on fine-scale gene flow within fire-suppressed populations of Pinus lambertiana, a historically dominant and ecologically important member of mixed-conifer forests of the Sierra Nevada, California. Here, I find evidence that treatment prescription differentially affects fine-scale genetic structure and effective gene flow in this species. In my third chapter, I describe the development of a dense linkage map for Pinus balfouriana which I use in chapter four to assess the quantitative trait locus (QTL) landscape of water-use efficiency across two isolated ranges of the species. I find evidence that precipitation-related variables structure the geographical range of P. balfouriana, that traits related to water-use efficiency are heritable and differentiated across populations, and associated QTLs underlying this phenotypic variation explain large proportions of total variation. In chapter five, I assess evidence for local adaptation to the eastern Sierra Nevada rain shadow within P. albicaulisacross fine spatial scales of the Lake Tahoe Basin, USA. Here, genetic variation of traits related to water availability were structured more so across populations than neutral variation, and loci identified by genome-wide association methods show elevated signals of local adaptation that track soil water availability. In chapter six, I review theory related to polygenic local adaptation and literature of genotype-phenotype associations in trees. I find that evidence suggests a polygenic basis for many traits important to conservation and industry, and I suggest paths forward to best describing such genetic bases in tree species. Overall, my results show that spatial and genetic structure of trees are often driven by their environment, and that ongoing selective pressures driven by environmental change will continue to be important in these systems.

This dissertation uses the constructivist grounded theory methods of Charmaz (2011) to explore: 1) the unique characteristics of landscape-scale collaboration; 2) implications for collaborative capacity-building strategies; and 3) the relationship between conflict, landscape-scale collaboration, and conflict resolution. The study was conducted through the US Forest Service's Collaborative Forest Landscape Restoration Program (CFLRP). In the 1980s and 1990s, national forest management conflicts brought the forest industry to a standstill, with many jobs lost. In addition, historic fire suppression practices have made our national forests highly vulnerable to catastrophic wildfire. Many have strong opinions about what should be done and how. The proposed substantive theory suggests landscape-scale collaboration can serve as a conflict prevention, problem solving, or conflict resolution venue and offer opportunities for remarkable efficiencies in forest restoration as well as profoundly restorative transformation in ecological, social, economic, personal, and spiritual dimensions. It identifies unique characteristics of collaboration at this scale; suggests that realizing benefits depends on collaborative capacities at the collaborator, constituent organization, collaborative stakeholder group, and sponsoring organization levels, and on mastering nine challenges; and suggests eight implications for collaborative capacity building strategies. The study contributes to forest restoration, reduced loss of life and livelihood, and economic recovery by contributing to CFLRP effectiveness. It contributes to the field of conflict resolution by: illuminating the collaboration / conflict resolution relationship; a particular application of collaboration; related sources of conflict; and conflict resolution strategies. It advances new directions of study for conflict resolution scholars--i.e., how to help agencies and groups strengthen their collaborative capacities.

River restoration projects are being installed worldwide to rehabilitate degraded river habitat. Many of these projects focus on stream habitat improvement (SHI), and an estimated 60%of the 37,000 projects listed in the National River Restoration Science Synthesis Program focus on SHI for salmon and trout species. These projects frequently lack a sufficient monitoring program or account for the environmental costs associated with SHI. The present study used life cycle assessment (LCA) techniques and topographic effectiveness monitoring to quantify environmental costs on the basis of geomorphic change. This methodology was a novel approach to assessing the cost-benefit relationship of SHI. To test this methodology, two phases of the Lower Scotts Creek Floodplain and Habitat Enhancement Project (LSCR) were used as a case study. The LSCR was a SHI project installed along the northern coast of Santa Cruz County, California, USA. A limited scope LCA was used to quantify the life cycle impacts of raw material production, materials transportation, and on-site construction. Once these baseline results were produced, a topographic monitoring program was used to quantify the topographic diversity index (TDI) in pre- and post-project conditions. The TDI percent change was used to scale the baseline LCA results, which quantified the environmental impacts based on geomorphic change. Phase II outperformed phase I. Phase I had greater cumulative environmental impacts and experienced a 7.7 % TDI increase from pre- to post-project conditions. Phase II had 43% less cumulative environmental impacts and experienced a 7.9% TDI increase from pre- to post-project conditions. The impacts in phase I were greater because of the amount of material excavated to create off-channel features, which were a key feature of the LSCR. A scenario analysis also was conducted within the LCA component of this study. The scenario analysis suggests that life cycle impacts could be reduced by 30%-65% by using the accelerated recruitment method in place of importing materials to build large wood complexes. The results of this study suggest that managers may improve the environmental performance of SHI projects by: (1) using the accelerated recruitment method to introduce larger key pieces to the channel, reducing the need to import materials; (2) using nursery grown plants as opposed to excavating plants for revegetation; (3) minimizing fuel combustion in heavy equipment and haul trucks by ensuring clear access to the channel and streambank, using small engine equipment to clear access corridors during site preparation, running more fuel-efficient machinery or bio-fuel powered machinery, and by attempting to minimize haul distances by sourcing materials locally; and (4) utilizing a “franken-log” design (a ballasted LWC configuration with a rootwad fastened to the downstream end of a log) in LWCs which led to favorable TDI change. This study concluded that LCA could be a valuable tool for monitoring SHI and river restoration projects and that further research of the TDI analysis is justified.

This research examines the relationship between understory plant diversity and logs in a Pacific Northwest (PNW) Sitka spruce (Picea sitchensis)-western hemlock (Tsuga heterophylla) old-growth, coastal forest. These forests are renowned for their high forest productivity, frequent wind storms, and slow log decomposition rates that produce unmatched accumulations of coarse woody debris (CWD) yet few studies have examined the relationship between CWD and understory vegetation ecology. My research addressed this topic by comparing understory plant census data between paired fallen log and forest floor sites (n=20 pairs). My objectives were to: 1) determine the influence of substrate type on community composition and diversity, and 2) examine successional pathways and species assemblage patterns on CWD in various stages of decomposition. To meet these objectives I employed non-metric multidimensional scaling (NMDS) ordinations and unsupervised cluster analyses to identify and compare community assemblages on both substrates. These methods revealed similar species diversity and evenness between log and forest floor sites with compositional differences within and between substrates corresponding to habitat availability for colonization and light and moisture gradients. My results also suggest understory successional pathways related to decay class and characterized by an initial abundance of bryophytes, forbs, and seedlings followed by woody shrubs. Understory communities developing on logs also experienced increasing diversity, evenness, and divergence from forest floor communities consistent with log decomposition. These results differ from findings for boreal forests that reveal increasing similarity between substrate communities with increasing decay class. Recommendations for future research include the employment of a more robust sample size and direct measurements of environmental variables. Additional comparator studies are also needed to confirm the effects of forest type and decomposition on the relationship between CWD and forest understory communities. This study demonstrates how fine-scale wind disturbance fosters biodiversity through the creation of CWD substrate. My results and future research are essential for the development of silvicultural models designed to promote biodiversity in PNW coastal forests.

A botanical inventory of Doe Mountain Recreation Area (DMRA) in northeastern Tennessee was conducted to help guide conservation-based management. A total of 484 species were found in DMRA, comprising 94 families, and 285 genera, 10 species listed in the state rare plant list, and 76 exotic species. Two species, Liatris virgata and Lycopodiella inundata, were new state records. Water in the Lycopodiella seep was an order of magnitude more acid than at other sites. An analysis of the wildland-urban interface showed that only 13% of the area was classified as uninhabited. The inventory-invasion index, introduced to quantify the relative degree of botanical uniqueness, was indicative of an under-explored or unique area but less so compared to some other botanically-rich regional sites. Management should aim to protect acid seeps, arid roadside slopes, curtail mowing a roadside that supports a state endangered species, and avoid herbicides in the biodiverse power line corridor.

Literature shows that production of cottonseed adhesives is feasible to develop an environmentally friendly and competitive bio-based wood adhesive. Defatted cottonseed and water-washed cottonseed meals were prepared from glandless cottonseed and were used in adhesive formulations to produce three-ply yellow poplar (Liriodendron tulipifera) plywood panels as the first objective. These two cottonseed meals were compared with the properties of plywood panels made with an adhesive formulated from a commercial soybean meal, as a control. Adhesive resins were prepared from each protein meal with sodium metabisulfite (Na2S2O5) and one of two polyamido-amine-epichlorohydrin (PAE) wet strength agents, and the plywood panels were produced by hot pressing for 7, 8.5 and 10 minutes at 135°C with a constant pressure of 1.241 MPa. Panels prepared from three protein meals had comparable shear strengths. The combinations of the two cottonseed preparations and the two wet strength agents produced panels with acceptable wet resistant properties, whereas the soybean meal only produced acceptable panels with one of the wet strength agents. Because the panels prepared from the two cottonseed meals had comparable properties, there appears to be no benefit to including a water-washing step to increase the meal’s protein level. The second objective of this research was to reduce the hot press time and develop cottonseed meals into adhesives to become comparable to commercial soybean-based adhesives. New cottonseed, water-washed cottonseed, and commercial in-house soybean meals were separately prepared with deionized water, sodium metabisulfite, and PAE to produce three-ply yellow poplar plywood panels. The panels were hot pressed for 4, 5, and 6 minutes at 135°C with a constant pressure of 1.241 MPa. Panels prepared from the three meals and commercial soybean plywood panels had comparable mechanical shear strengths and water resistance properties. Results indicated that press time, meal types, and interactions were statistically significant. Shear strength results indicate that cottonseed could be used alternatively to soybean. The new cottonseed panels were more resistant to delamination than soybean. The cottonseed meals showed great promise for applicability as a formaldehyde-free, bio-based, and environmentally friendly hardwood plywood wood-based adhesives product for use in interior type applications.