Nous avons identifié une association poly(ADP-ribose) (PAR)-dépendante entre PARP1 et UHRF1. UHRF1 est PARylé par PARP1 et lie le PAR de façon non covalente. L’absence de PARP1 (i) perturbe l’association de UHRF1 et DNMT1, (ii) induit une ubiquitination excessive de DNMT1 par UHRF1 favorisant sa dégradation au cours du cycle, (iii) favorise la transcription des régions de l’hétérochromatine péricentrique (pHC) (iv) et perturbe la localisation de la marque répressive H4K20me3 au niveau des foyers de l’pHC. Dans un deuxième temps, nous avons étudié le rôle de l’association KAP1-HP1 dans la réponse cellulaire aux dommages. L’interaction entre ces deux partenaires est essentielle pour le recrutement de KAP1 sur les sites de cassures. Après induction de cassures, l’absence d’interaction induit un délai dans la réparation des cassures double-brins et une diminution de la survie cellulaire. Une analyse détaillée suggère une déficience du mécanisme de réparation par recombinaison homologue. / We identified a poly(ADP-ribose) (PAR)-dependent interaction between PARP1 and UHRF1. UHRF1 is PARylated by PARP1 and binds PAR in a non-covalent way. The absence of PARP1 (i) impairs the UHRF1/DNMT1 interaction, (ii) induces excessive UHRF1-mediated ubiquitination of DNMT1 promoting its degradation during the cell cycle, (iii) increases the transcription of pericentric heterochromatin (pHC) regions (iv) and impairs the localization of the repressive histone mark H4K20me3 on pHC. In a second project we studied the role of the KAP1/HP1 interaction in response to DNA damage. The interaction between the two partners is essential for KAP1 recruitment to DNA damage sites. The absence of the interaction, after damage, induces a delay of the double strand break repair kinetics and decreases the cell survival rate. A more detailed analysis suggests a deficiency of the homologous recombination repair pathway.

With the advancement in the current wireless technology standards such as terrestrial digital video broadcasting systems (DVB-T, DVB-T2, and DVB-H) and the massive usage of the Internet over mobile devices, streaming of television channels in smart phones has become a necessary advancement for mobile users. As, UMTS dominating the entire mobile market globally and with the evolution of LTE, several mobile operators are working on an MBMS framework which will help to launch mobile TV services on respective operators. This paper deals with terrestrial and mobile TV with IP multicasting and broadcasting and is aimed to improve system spectral efficiency.  With the help of IP multicasting, the base station can be able to provide with significantly less spectrum by saving it from the channels which the user is not viewing currently. This case is analysed from several sets, called schemes of resource plan sets. The transmitter scheduling is dealt with by means of a Packet and Resource Plan Scheduling (PARPS) algorithm, and the simulated results are plotted in Matlab which assists in analysing the efficiency in the spectrum management and the coverage probability for the number of transmitters used for each scheme. The schemes are simulated in Matlab for different number of transmitters (2-7) in both the static and random model. The SFN schemes are offering greater coverage probability than MFN schemes, in all cases. Multicasting over Continuous Transmission Dynamic Single Frequency Network (CT-DSFN) offers a 1342% and 582% gain in Multi-user System Spectral Efficiency (MSSE) for 7 transmitters, from Broadcasting over MFN and Broadcasting over SFN respectively.  For 7 transmitters, Multicasting over CT-DSFN offers a 1213% and 428% gain in System Spectral Efficiency (SSE) from Broadcasting over MFN and Broadcasting over SFN respectively.