Germination, respiration, and photosynthesis in seeds of dwarf mistletoe (Arcenthobium) were studied. The effects of 1 hour soakings of seeds in aqueous solutions of 1, 2, or 3% H2O2 or 1, 2, 3, 4, or 5% Chlorox on germination of seeds were tested. Germination rates for seeds from three consecutive years (1975-1977) were obtained. Germination rates varied widely. This variance transcended subtle changes in treatment. Pretreatment of seeds with Chlorox resulted in significantly lower germination rates than those of the control. Chlorophyll concentrations were determined for seeds and aerial shoot tissue of four species. Seeds of A. douglasii had the highest chlorophyll concentration (0.39 mg/g fresh weight) while seeds of A. tsugense had the lowest concentration (0.25 mg/g fresh weight). Net O2 uptake by seeds of A. campylopodum in dark and in light was measured by manometric and polarographic methods. The mean values were 353 μl O2 g-1 h-1 in the dark and 201 O2 g-1 h-1 in the light. The difference between these rates is apparently due to O2 evolution during photosynthesis. In light the seeds can fix 43% of the CO2 produced by respiration. Experiments in which seeds were exposed to 14CO2 in light confirmed that the seeds are able to fix CO2. Extraction of seeds with ethanol showed that 97-99% of the incorporated 14C was ethanol soluble. Ten to sixteen percent of the ethanol fraction was chloroform soluble while the rest was H2o soluble. Ion exchange separation of the H2o phase showed that 11-25% of 14C activity was cationic, 15-29% anionic, and 53-67% neutral.

京都大学 / 0048 / 新制・課程博士 / 博士(工学) / 甲第21074号 / 工博第4438号 / 新制||工||1690(附属図書館) / 京都大学大学院工学研究科分子工学専攻 / (主査)教授 田中 庸裕, 教授 梶 弘典, 教授 今堀 博 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DFAM

Temperature is a major factor affecting plant growth and development at all stages. The objectives of this study were to investigate the temperature effects on all aspects of soybean growth and development. Five experiments were conducted at a wide range of temperatures under optimum water and nutrient conditions at specific growth stages. Two cultivars, Asgrow AG5332 (AG) and Progeny P5333RY (PR) from MG V with different growth habits, were used. All studies except the seed germination were conducted in sunlit plant growth chambers under optimum water and nutrient conditions. The seed germination experiment was conducted in a temperature-controlled incubator. Germination traits were measured during seed germination studies. In other experiments, plant growth, developmental rates, gas exchange parameters, and seed yield and quality were measured. Cultivars did not differ for seed germination and emergence traits. Quadratic functions best described time to 50% germination seed germination and emergence rates. The three cardinal temperatures for seed germination were 8.56°C (base) 27. 96°C (optimum) and 46.92°C (maximum). The base and optimum temperature for seed emergence were 10.6 and 36.7°C, respectively. During the early-season, 0-21 days, root and shoot growth parameters responded similar temperature responses, the root traits have a lower optimum (29.91°C) than the shoot parameters (36.56°C). Cultivars varied in their response to temperature during vegetative development, particularly for growth parameters, and the temperature optimums for various parameters. Node addition rate was not different among the cultivars but increased with increase in temperature. The flowering time (R1) from emergence showed quadratic trends with an increase in temperature up to 28°C and increased slightly at the higher temperatures. The PR cultivar, on an average, took 15 additional days to reach flowering compared AG cultivar across temperatures. Pod and seed yield and individual seed weight and harvest indices showed quadratic trends with maximum values at 25.82 °C for AG and 23.36 °C for PR. The functional algorithms could be helpful for management and in improving crop models.

No description available.

No description available.

Regulated deficit irrigation (RDI) is a common and useful practice for water conservation and improving grape quality. To attain better grape characteristics and wine quality, a substantial degree of irrigation stress is intentionally allowed to occur during the first part of berry formation and can continue until later into veraison. Understanding the effects of deficit irrigation on photosynthetic rates could be helpful in determining at what degree and duration a grower should perform this irrigation practice. The focus of this study was to determine the effects of using differing degrees of RDI in a vineyard located in Paso Robles, California (central coast region) on gas exchange of the Syrah variety. The target irrigation levels were set for each season at 75, 60, 45, and 30% ETc of a fully irrigated vine (100% ET). The 60% replication was considered as the control for this study, as it is the standard target ET rate for the vineyard where this study was conducted. A gas analyzer (LICOR 6200) was used to measure the overall rate of photosynthesis during two successive growing seasons (2004 & 2005). Measurements were taken from bloom through harvest and were compared among the four different irrigation levels. Along with overall photosynthetic rate, the leaf temperature, stomatal conductance, light level, and relative humidity were also measured. The results of the two year study were statistically compared through an analysis of variance (ANOVA) and analyzed for their significance. The results of the study showed that minor differences in the mean photosynthetic rates were found to occur during brief periods of the growing season. These differences ranged from 1-4 weeks and did not occur at similar times of the growing season. However, no statistical significant differences were found to exist when compared among the four irrigation levels for the entire growing season. Observed differences in canopy sizes indicated that irrigation amounts had affected the overall growth to some degree during this two year study. Several plant physiological measurements showed a significant difference in the measured gas exchange rates between sun exposed leaves and the shade leaves within the treatment area. A significant correlation of the effect of leaf temperature on stomatal conductance was observed to exist in one of the irrigation treatments (45% ET) during this study. Other plant physiological measurements indicated that highly significant differences existed between the photosynthesis rate and leaf temperature. Photosynthetic rates were highly significantly correlated to leaf conductance, air temperature, and relative humidity. A significant difference of photosynthetic rates was identified to occur between stomatal conductance and air temperature. This study concludes that differential irrigation amounts on Syrah in the Central Coast region, specifically Paso Robles, have minimal effect on overall photosynthetic rate and does not fully support the anisohydric stomatal reaction that has recently been studied by plant physiologists working with this variety.

Modern microbialites provide the opportunity to explore the influences of biology on microbialite formation and understand how biosignatures can be preserved in these structures. In this study, we compared δ13Ccarb values from nodule and surface biofilm carbonates on microbialite structures across depths and locations throughout Pavilion Lake to evaluate whether variable light exposure produced limitations to biosignature formation. At depths below 21 m, vertical profiling of δ13Ccarb across colour transitions of surface biofilm on microbialite structures was performed to identify spatial arrangement of autotrophic and heterotrophic biosignatures. Finally, preservation of the photosynthetic biosignature over time was investigated by collecting carbonates beneath the microbialite surface. These investigations were performed in order to better characterize the factors controlling biosignature formation, distribution, and preservation within Pavilion Lake.Decreasing trends of δ13Ccarb with depth across study sites indicated that attenuated sunlight in the water column is likely the primary control on biosignature formation. Below 21 m, photosynthetic enrichments representing biosignatures on microbialite surfaces were reduced and recorded δ13Ccarb values that fell within the predicted equilibrium range. Biosignature loss is suggested to result from the relative proportions of autotrophic and heterotrophic processes changing at depths and producing average δ13Ccarb values. Variability of where biosignatures are lost on the microbialite surface indicated that the spatial extent of photosynthetic communities producing enrichments is potentially influenced by variable incidences of light at these depths. Although no definitive biosignatures of heterotrophy were identified, several interfaces were identified where the balancing proportions of autotrophic and heterotrophic processes influenced by light variability potentially mediate biosignature loss. Decreasing trends of δ13Ccarb beneath the microbialite surface and estimates of past microbialite growth rates indicated that surface biosignatures are lost within 100 – 400 years. It is suggested that infilling processes overprint enrichments and deplete δ13Ccarb values due to heterotrophic abundance below the microbialite surface. This is supported by an isotopic mass balance that predicts smaller inputs of heterotrophically-depleted DIC are required to sufficiently overprint δ13Ccarb enrichments. These results concluded that the photosynthetic biosignature identified in Pavilion Lake is short-lived and mitigated by biological processes. / Thesis / Master of Science (MSc)

Bubbles of oxygen from Elodea canadensis were collected and found 5 per mil enriched in 0-18. This confirms the results of both Dole and Vinogradov, who found 0-18 enrichment of photosynthetic oxygen relative to its source, i.e. water. Photosynthesis and respiration appear to have produced the Dole effect, i.e. the 22 per mil 0-18 enrichment of atmospheric oxygen relative to ocean water, its probable source. These results are contrary to findings for both UV photolysis and electrolysis of water both evolving oxygen found depleted in 0-18.

No description available.

No description available.