Enhancing the performance of mixing and fluid entrainment by excitation of quasi-steady jets has been a subject of research for more than three decades. During the 1980s a special emphasis was placed on mechanically oscillating planar jets and the possibility to augment thrust of V/STOL aircraft. However, during this time, little attention was paid to the classification of flow regimes, the development of coherent structures or the existence of different regions in the flow within the jet near field. For the present study, a large aspect ratio nozzle was oscillated in the direction transverse to the width of the nozzle in simple harmonic motion. For a constant nozzle height, the stroke length, oscillation frequency and jet velocity were systematically varied. Over 240 flow cases were examined using a novel method of phase-locked flow visualisation. Following an initial analysis of the acquired data, a small subset of flow conditions was selected for further quantitative investigation using Particle Image Velocimetry (PIV). The phase-locked flow visualisation led to the identification and classification of three separate flow regimes, the Base Flow, the Resonant Flow and the Bifurcation Flow Regimes. Each regime is linked to the other regimes by the presence of a small number of repetitive coherent structures in the form of starting and stopping vortices. The analysis revealed a relationship between the stroke-to-nozzle height ratio and the ratio of the forcing frequency to the natural vortex shedding frequency in the planar jet. This directly contradicts the relationship between the Strouhal and Reynolds numbers of the jet that was proposed by previous investigators. Comparison of phase-locked PIV and flow visualisation data confirms both, the validity of the new regime classification and the identification of relevant large-scale structures. Time-averaged vorticity data are also used to further illustrate the differences between the three flow regimes. Investigation of the time-averaged qualitative data for the Base and Resonant Flow Regimes show that three distinct flow regions exist within both regimes. Adjacent to the nozzle is the initial formation region, where all large-scale structures form. This is followed by a coherent near-field region in which the jet exhibits very little spread for both the Base and Resonant Flow Regimes. Within this region no pairing of the large-scale vortices from the opposing sides of the flow can be found. This region is followed by a transition region that is marked by the sudden breakup and dissipation of all visible large-scale coherent structures. The vortex formation distance is then investigated using the available PIV data and compared with the results of previous investigations. The data show that the formation distance depends on the jet velocity, oscillation frequency and the stroke length. The agreement with previous data is poor due to differences in the method of measurement. Quantitative data are also used to investigate the centreline velocity decay in relation to changes of the jet Reynolds number and stroke-to-nozzle height ratio. The results show that the velocity decay rate increases with increasing stroke length as is expected from findings of earlier studies. In addition the centreline velocity decay rates in the mean jet transition region appear to be constant for each stroke length in the cases examined. Finally, conclusions are drawn and recommendations for future work are presented. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1349701 / Thesis (Ph.D.) -- University of Adelaide, School of Mechanical Engineering, 2009

Orientador: Eunice Oba / Resumo: Os meios diluidores de sêmen exercem importante papel na manutenção da qualidade e da viabilidade espermática, assim como da sua capacidade de fertilização após o processo de criopreservação. O estudo objetivou avaliar a eficácia do caseinato de sódio (2%) adicionado a diluente comercial com 15% de gema de ovo (grupo GC), na manutenção da fertilidade do sêmen bovino criopreservado e, como controle, empregou-se o mesmo diluente comercial com 20% de gema de ovo sem caseinato de sódio (grupo G). No experimento 1, em sêmen descongelado dos dois grupos foram avaliados padrões de cinética espermática, população de espermatozoides sem as alterações das membranas plasmática e acrossomal (IMPA), sem desestabilização da membrana (SDM) e sem translocação de fosfatidilserina da membrana (STF), alto potencial mitocondrial (APM) e geração de superóxido (O2-). Nos experimentos 2 e 3 realizaram-se respectivamente, teste de fertilidade in vitro (taxa de clivagem no dia 3 e taxa de formação de embriões no dia 8 após a fecundação) e in vivo (porcentagem de prenhez) por meio da IATF. De acordo com os resultados, verificou-se diferença na cinética espermática entre os dois grupos, a favor do grupo GC (P<0,05) principalmente após estresse térmico (T90). Com relação às avaliações da IMPA, SDM, STF, observaram-se diferenças também no T90 em favor do grupo GC (P<0,05) para as três avaliações. Contudo, o alto potencial mitocondrial (APM) e as espécies reativas ao oxigênio (EROS) foram menores no T0 e ... (Resumo completo, clicar acesso eletrônico abaixo) / Doutor

Cette thèse de doctorat a été réalisée dans le cadre du projet HARVEST, programme de recherche initié en 2001 au LEGI et consacré au développement d'un nouveau concept d'hydrolienne à axe vertical inspiré des turbines Darrieus pour la récupération de l'énergie cinétique des courants marins et fluviaux. Ce travail s'est focalisé sur la mise en place d'un moyen de mesure par Vélocimétrie par Image de Particules deux dimensions – deux composantes (2D-2C) et deux dimensions – trois composantes (2D-3C). L'objectif est d'une part de constituer une base de données expérimentale pour la validation locale des simulations numériques RANS 2D et 3D menées dans le cadre de travaux précédents, et d'autre part d'améliorer la compréhension des phénomènes hydrodynamiques instationnaires rencontrés dans ces machines et en particulier du décrochage dynamique. La confrontation des mesures expérimentales et des simulations a notamment permis de mettre en évidence les points forts et les limites des modèles numériques dans les différents régimes de fonctionnement de la machine. / This PhD thesis has been carried out within the framework of the HARVEST project. This research program, initiated in 2001 by the LEGI laboratory (Grenoble, France), is devoted to the development of a new marine turbine concept inspired from Darrieus turbines in order to convert kinetic energy of marine, tidal or river currents into electric energy. This work has been focused on the development of an experimental apparatus based on two dimensions – two components (2D-2C) and two dimensions – three components (2D-3C) Particle Image Velocimetry. The objective is to provide an experimental database for the local validation of 2D and 3D RANS computations and to improve our understanding of unsteady hydrodynamics phenomena experienced in this type of turbine and especially of dynamic stall. Comparison between measurements and computations enabled to identify strengths and limitations of numerical models for various operating conditions of this type of turbine.

Made available in DSpace on 2018-08-02T00:02:59Z (GMT). No. of bitstreams: 1 tese_11317_ALAN VICTOR FERREIRA MODOLO .pdf: 4918358 bytes, checksum: 4f9210c521179af173132f3ad0f9737f (MD5) Previous issue date: 2017-03-24 / Ao longo do último século, o Dam Break Flow vem sendo estudado por muitos autores. A compreensão deste tipo de fenômeno é de grande relevância, uma vez que envolve aplicações em barragens que oferecem múltiplos benefícios para a humanidade. Porém, o rompimento de uma dessas barragens pode trazer consequências indesejáveis e imensuráveis para a sociedade e para o setor de mineração, por exemplo. Neste trabalho estamos interessados em entender como o número de Froude e as propriedades não-newtonianas, particularmente a viscoplasticidade, afetam o perfil de interface do escoamento e a distância alcançada pelo material após o Dam Break Flow. Tais resultados são comparados com o deslocamento de um fluido newtoniano. Através de uma simples abordagem experimental, uma solução de material viscoplástico ou newtoniana incialmente armazenada em um reservatório é instantaneamente liberada. As imagens do escoamento são capturadas durante o teste de duas maneiras diferentes. Uma é feita utilizando uma câmera CCD e a outra é através da Velocimetria por Imagem de Partícula. A partir das imagens obtidas pela câmera CCD, obtém-se um mapa do escoamento, com as suas respectivas velocidades, nível de líquido, formato das interfaces ar-líquido e distância percorrida pelo material são observadas. Já os resultados obtidos com o PIV, são para determinar o campo de velocidade dos momentos iniciais do Dam Break Flow para os diferentes tipos de materiais estudados. Neste trabalho, é mostrado que a plasticidade do material tem total influência sobre a distância alcançada pelo escoamento e também resultam em instabilidades ao longo do mesmo. Foi ainda identificado que o fluido viscoplástico alcança velocidades iniciais maiores que o caso newtoniano. Porém, no decorrer do escoamento, decrescem rapidamente. Quando analisado o caso viscoplástico para um leito com rugosidade, nota-se que para uma superfície lisa, há deslizamento do material de forma significativa para altos valores de plasticidade. Através da análise com PIV, foi constatado que a velocidade de frente de onda é um pouco menor do que a velocidade máxima obtida pelo escoamento. Palavras chave: Dam Break Flow, material viscoplástico, PIV, plasticidade, deslizamento.

Despite significant recent progress, dense, time-resolved imaging of complex, non-stationary 3D flow velocities remains an elusive goal. This work tackles this problem by extending an established 2D method, Particle Imaging Velocimetry, to three dimensions by encoding depth into color. The encoding is achieved by illuminating the flow volume with a continuum of light planes (a “rainbow”), such that each depth corresponds to a specific wavelength of light. A diffractive component in the camera optics ensures that all planes are in focus simultaneously. With this setup, a single color camera is sufficient to track 3D trajectories of particles by combining 2D spatial and 1D color information. For reconstruction, this thesis derives an image formation model for recovering stationary 3D particle positions. 3D velocity estimation is achieved with a variant of 3D optical flow that accounts for both physical constraints as well as the rainbow image formation model. The proposed method is evaluated by both simulations and an experimental prototype setup.

Les mécanismes d’évolution spatio-temporelle des structures turbulentes instationnaires tridimensionnelles, et en particulier ceux rencontrés aux plus grandes échelles, sont à l’origine de phénomènes d’instabilité qui conduisent très souvent à une diminution de la performance des systèmes énergétiques. C’est le cas des variations cycle-à-cycle dans le moteur à combustion interne. Malgré les progrès substantiels réalisés par la simulation numérique en mécanique des fluides, les approches expérimentales demeurent essentielles pour l’analyse et la compréhension des phénomènes physiques ayant lieu. Dans ce travail de thèse, deux types de vélocimétrie par image de particules (PIV) ont été appliqués et adaptés au banc moteur optique du laboratoire Coria pour étudier l’écoulement en fonction de six conditions de fonctionnement du moteur. La PIV Haute Cadence 2D2C a permis d’abord d’obtenir un suivi temporel de l’écoulement dans le cylindre durant un même cycle moteur ainsi qu’identifier ces variations cycliques. La PIV Tomographique 3D3C a permis ensuite d’étendre les données mesurées vers l’espace tridimensionnel. La Tomo-PIV fait intervenir 4 caméras en position angulaire visualisant un environnement de géométrie complexe, confinée, ayant un accès optique restreint et introduisant des déformations optiques importantes. Cela a nécessité une attention particulière vis-à-vis du processus de calibration 3D des modèles de caméras. Des analyses conditionnées 2D et 3D de l’écoulement sont effectuées en se basant principalement sur la décomposition propre orthogonale (POD) permettant de séparer les différentes échelles de structure et le critère Γ permettant l’identification des centres des tourbillons. / The unsteady evolution of three-dimensional large scale flow structures can often lead to a decrease in the performance of energetic systems. This is the case of cycle-to-cycle variations occurring in the internal combustion engine. Despite the substantial advancement made by numerical simulations in fluid mechanics, experimental measurements remain a requirement to validate any numerical model of a physical process. In this thesis, two types of particle image velocimetry (PIV) were applied and adapted to the optical engine test bench of the Coria laboratory in order to study the in-cylinder flow with respect to six operating conditions. First, the Time-Resolved PIV (2D2C) allowed obtaining a temporal tracking of the in-cylinder flow and identifying cyclic variabilities. Then tomographic PIV (3D3C) allowed extending the measured data to the three-dimensional domain. The Tomo-PIV setup consisted of 4 cameras in angular positioning, visualizing a confined environment with restricted optical access and important optical deformations. This required a particular attention regarding the 3D calibration process of camera models. 2D and 3D conditional analyses of the flow were performed using the proper orthogonal decomposition (POD) allowing to separate the different scales of flow structures and the Γ criterion allowing the identification of vortices centres.

In this study, an experimental investigation was performed to characterize the dynamic stall of pitching wings and provide confirmation of the existence of the arch-shaped vortex for moderate sweep wing. Dynamic stall is a complex flow, which happens because of a sudden change of incident angle during the pitching motion. The pitching motion of a wing can cause instability in the shear layer and generate the separation burst at certain angles. For a pitching wing, the dynamic stall vortex is characterized by the formation of an arch-shaped vortex to the evolution of a ring-shaped vortex. The leg of the arch-shaped vortex causes a negative pressure region on the airfoil surface and can, in fact, generate greater lift. However, in certain conditions, the detachment of the arch-shaped vortex from the airfoil surface can cause high pressure and vibration in the structures. The formation of the arch-shaped vortex and its evolution were systematically investigated using cutting-edge flow diagnostic techniques, and the physics of the dynamic stall is explained in addition to providing the confirmation of the theory developed based on Computational Fluid Dynamics. The study was done using Particle Image Velocimetry (PIV) and Pressure-Sensitive Paint for three sweep angle wings. The wings, with an aspect ratio of AR=4 and a NACA 0012 section assembled with round-tip, are considered for the current experimental study. The sweep angles = 0, 15, and 30 degrees were considered to compare the flow phenomena. The PIV results show the formation of a laminar separation bubble and its evolution to a dynamic stall vortex. The increase of sweep angle causes the formation of such vortices towards the wing tip. In the process of finding the footprint of the vortices and pressure distribution on the surface of the wings, a single-shot lifetime method using fast porous paint was used. The results show the existence of suction pressure and later grows towards the trailing edge of the wing due to the formation of a dynamic stall vortex. In addition, at Re=2x10^5 and reduced frequency k=0.2, a moderate sweep airfoil shows the apparent footprint of the arch-shaped vortex, which confirms the current theory.

Over the last 30 years, Particle Image Velocimetry (PIV) has become the most powerful tool to study velocity fields in fluid mechanics. This technique is non-intrusive requiring seeding the flow with small tracer particles. The hardware required for these sophisticated PIV methods is very expensive (CCD or CMOS high-speed cameras and lasers), and the present dissertation aims to develop novel and inexpensive alternatives. The first part of this work investigates the use of multiple smartphones as a lower-cost Tomographic-PIV system for reconstructing 3D-3C velocity fields. We use colored shadows to imprint two or three different time-steps on the same image in a RGB-backlit configuration. We use commercially available Tomo-PIV software for the calibration, 3-D particle reconstruction, and particle-field correlations, to obtain three velocity components in a volume. The proposed system is tested with a vortex ring and the results are compared to stereoscopic-PIV for error estimations. We expand this work to a high-speed time-resolved setup to obtain 3D-3C velocity fields in time. This improvement is possible using newer smartphones capable of recording high-speed video at HD resolution. The challenges of using such cameras are presented and tackled. The illumination system, testing flow and image processing is similar to the one presented in the first section. A benchmark of the smartphone system is carried out comparing it to a Tomo-PIV system capable of recording 4K video resolution. A different approach is proposed to reconstruct a 3D-3C velocity field using a single color video camera. This technique uses chromatic structured light with color-gradients projected perpendicularly with respect to the color camera. Thus, we encode the depth position of the particles with a different wavelength of light. Different light sources are used to produce such color gradients. Finally, a variation of the previous technique is tested using a single monochromatic camera and structured volumetric illumination with spatially varying intensity profiles. This technique enables us to encode the depth position of every particle in their intrinsic brightness. The proposed system can achieve a depth resolution of 200 levels, i.e., an order of magnitude higher than previously proposed systems.

No description available.

An in vitro arterial flow bioreactor system for the generation of physiological flows in a biological environment was designed, constructed, and characterized. The design was based on models previously used to investigate the response of endothelial cells to shear. The model interfaces a bioreactor with flow elements to compose a flow loop that reproduces arterial flow conditions within the bioreactor. High-resolution (8.6 microns) time-resolved (4 ms) velocity field measurements within the bioreactor were obtained using Particle Image Velocimetry (PIV). Two physiological flows were considered, corresponding to medium human arteries at rest and exercise conditions: first, with an average Reynolds number of 150 and a Womersley parameter of 6.4, and second, with an average Reynolds number of 300 and a Womersley parameter of 9.0. Two cases were considered: first, using a smooth artery section, and second, with a confluent layer of human microvascular endothelial cells grown on the inner surface of the artery section. The instantaneous wall shear stress, time-averaged wall shear stress, and oscillatory shear index were computed from the velocity field measurements and compared for the cases with and without cells. These measurements were used to assess the value of the system for measurement of correlations between fluid dynamics and the response of biological tissue. It was determined that the flow present in such a system is not an accurate reproduction of physiological flow, and that direct measurement of the flow is necessary for accurate quantification of cellular response to fluid parameters. / Master of Science