Master of Science

Lincosamides form a small but important group of specialized microbial metabolites with antibiotic activity. The most important members of this group are celesticetin and clinically used lincomycin. Structurally, lincosamides are composed of an amino sugar and an amino acid connected by an amide bond. The amino acid precursors of both lincosamides remarkably differ. Proteinogenic L-proline is the precursor of celesticetin, while an unusual amino acid (2S,4R)-4-propyl- L-proline (PPL) is incorporated in the more efficient compound lincomycin. Surprisingly, both these precursors are recognized and activated for further biosynthetic steps by homologous adenylation domains CcbC and LmbC, respectively. The detailed description of this amino acid recognition and activation step, which is critical for the biological activity of the resulting compound, was the aim of the first part of this thesis. The site-directed mutagenesis of the LmbC substrate binding pocket and biochemical characterization of resulting mutants were employed to identify the residues crucial for the activation of PPL. Subsequently, we experimentally simulated the molecular evolution leading from L-proline-specific substrate binding pocket (like in CcbC) to the PPL-specific enzyme (LmbC). The substitution of only three amino acid...

Submitted by JOSETE BISPO RIBEIRO (josetebisporibeiro@gmail.com) on 2016-09-08T10:48:46Z No. of bitstreams: 1 Tese JOSETE BISPO RIBEIRO.pdf: 1208356 bytes, checksum: 9aea1bbdc5a48aa8bd8070c08b2b5d99 (MD5) / Rejected by Maria Auxiliadora da Silva Lopes (silopes@ufba.br), reason: Prezada Josete, Preencha os campos somente com as iniciais maiúsculas, exceto os nomes próprios. Saudações, Auxiliadora on 2016-09-08T16:37:56Z (GMT) / Submitted by JOSETE BISPO RIBEIRO (josetebisporibeiro@gmail.com) on 2016-09-09T01:45:30Z No. of bitstreams: 1 Tese JOSETE BISPO RIBEIRO.pdf: 1208356 bytes, checksum: 9aea1bbdc5a48aa8bd8070c08b2b5d99 (MD5) / Approved for entry into archive by Maria Auxiliadora da Silva Lopes (silopes@ufba.br) on 2016-09-09T17:50:54Z (GMT) No. of bitstreams: 1 Tese JOSETE BISPO RIBEIRO.pdf: 1208356 bytes, checksum: 9aea1bbdc5a48aa8bd8070c08b2b5d99 (MD5) / Made available in DSpace on 2016-09-09T17:50:54Z (GMT). No. of bitstreams: 1 Tese JOSETE BISPO RIBEIRO.pdf: 1208356 bytes, checksum: 9aea1bbdc5a48aa8bd8070c08b2b5d99 (MD5) / Esta investigação estuda a funcionalidade dos jogos digitais como instrumentos pedagógicos voltados para promover aprendizagem colaborativa em segurança pública. Para isso, focalizou a abordagem sobre os jogos do tipo Role Playing Game (RPG), para construir uma aplicação pedagógica, numa versão digital, denominada RPG Digital PMBA, voltada para utilização nos processos instrucionais da Polícia Militar da Bahia (PMBA). Identificou-se uma representação virtual e operabilidade lógica mais adequada à apresentação de ocorrências policiais militares, que, face à perspectiva decisória, promovessem nos profissionais policiais militares aperfeiçoamento de competências profissionais inerentes ao seu modus operandi. Este instrumento pedagógico, construído colaborativamente entre analistas cognitivos e especialistas em segurança pública, foi aplicado para teste de efetividade com os policiais militares do Batalhão Especializado de Polícia Turística (BEPTUR), unidade operacional vinculada à PMBA. Por se tratar de uma pesquisa aplicada, foram realizados experimentos reais, através de simulações virtuais de ocorrências policiais militares similares, em conteúdo especifico e narrativas usuais, àquelas encontradas no campo de atuação natural dos sujeitos da comunidade de prática. Considerando trata-se de uma investigação sobre tecnologias educativas, utilizou-se da abordagem metodológica Design Based Research (DBR), como método de pesquisa e dos pressupostos da Praxiologia, como concepção epistemológica, tendo em vista a indissociável relação formativa e instrucional entre o conhecimento teórico e a experiência prática, típica de profissionais da área de segurança pública. / ABSTRACT This investigation studies the funcionality of digital games how pedagogical instrument to promove collaborate knowledge in public security. Then, the focused approach about the games how role playing games (RPG), to build one pedagogical application, in a digital version, meaning RPG PMBA digital, to use instructions process of military police of Bahia (PMBA). It identified a virtual representation and logic operation more appropriate to submit police reports that, given operative perspective, to promote improvement of professional competencies inherents to yours. This pedagogical tool built collaboratively between cognitive analysts and public security experts, was applied to effectiveness test with militaries polices of special touristic police group (BEPTUR), operation linked to the PMBA, because it is an applied research, real experiments were performed through virtual simulations of similar military police reports, on specific content and the usual narratives, those found in the natural field of activity of the subjects of the community practice. Whereas it is a research on educational technology was used the methodological approach (DBR), as research method and praxeology of assumptions, such as epistemological conception, in view of the indissoluble training and institutional relationship between theoretical knowledge and practical experience, typical public stafety professionals area.