Distribution feeders form the last leg of the bulk power system and have the responsibility of providing reliable power to the customers. These feeders experience voltage drops due to a combination of feeder length, load distribution, and other factors. Traditionally, voltage drop was a major concern. Now, due to an ever-increasing PV penetration, overvoltage has also become a major concern. This limits the amount of solar PV that may be integrated. Few solutions exist to improve the voltage profile, where the most common is the use of voltage control devices like shunt capacitors and voltage regulators. Due to a large number of design parameters to be considered, the determination of the numbers and locations of these devices is a challenging problem. Significant research has been done to address this problem, utilizing a wide array of optimization techniques. However, many utilities still determine these locations and numbers manually. This is because most algorithms have not been adequately validated. The validation of a voltage profile design (VPD) algorithm has been presented here. The validation of this algorithm was carried out on a set of statistically relevant feeders. These feeders were chosen based on the results obtained from a feeder taxonomy study using clustering analysis. The algorithm was found to be effective in enhancing the amount of solar PV a feeder may host, while still maintaining all the voltages within the ANSI standard limits. Furthermore, the methodology adopted here may also be used for the validation of other algorithms. / Master of Science / Utilities have the responsibility of providing reliable power supply to their customers. Traditionally, bulk power was generated and transmitted over long distances incurring losses and voltage drops along the way. Now, with the integration of distributed energy resources, particularly solar photovoltaic (PV) generators at the customer locations, overvoltage has also become a problem. This requires adoption of measures which can help in maintaining the voltages within standard limits. Several options exist to compensate for these voltage issues, the most commonly used is voltage control devices like capacitor banks and voltage regulators. However, determining the required numbers of these devices and their appropriate locations is a challenging problem. Even though a number of algorithms have been proposed to give automated solutions to this problem, most utilities still use a manual approach. This is because these algorithms have not been validated on a statistically relevant set of feeders. To solve this issue, the validation of a voltage profile design (VPD) algorithm is presented in this thesis. The ability of this algorithm to enhance the amount of PV that may be connected to a distribution network has been validated on a set of feeders. The feeders were chosen based on the results obtained from clustering analysis, a machine learning concept. The cost effectiveness of this algorithm has also been investigated and significant savings were observed. Furthermore, the methodology adopted here can be easily extended for the validation of other algorithms as well.

Solar PV-based distributed generation has increased significantly over the last few years, and the rapid growth is expected to continue in the foreseeable future. As the penetration levels of distributed generation increase, power systems will become increasingly decentralized with bi-directional flow of electricity between the transmission and distribution networks. To manage such decentralized power systems, planners and operators need models that accurately reflect the structure of, and interactions between the transmission and distribution networks. Moreover, algorithms that can simulate the steady state and dynamics of power systems using these models are also needed. In this context, integrated transmission and distribution system modeling and simulation has become an important research area in recent years, and the primary focus so far has been on studying the steady state response of power systems using integrated transmission and distribution system models. The primary objective of this dissertation is to develop an analysis approach and a program that can simulate the dynamics of three phase, integrated transmission and distribution system models, and use the program to demonstrate the advantages of evaluating the impact of solar PV-based distributed generation on power systems dynamics using such models. To realize this objective, a new dynamic simulation analysis approach is presented, the implementation of the approach in a program is discussed, and verification studies are presented to demonstrate the accuracy of the program. A new dynamic model for small solar PV-based distributed generation is also investigated. This model can interface with unbalanced networks and change its real power output according to the incident solar irradiation. Finally, application of the dynamic simulation program for evaluating the impact of solar PV units using an integrated transmission and distribution system model is discussed. The dissertation presents a new approach for studying the impact of solar PV-based distributed generation on power systems dynamics, and demonstrates that the solar PV impact studies performed using the program and integrated transmission and distribution system models provide insights about the dynamic response of power systems that cannot be obtained using traditional dynamic simulation approaches that rely on transmission only models. / Ph. D. / To ensure that electricity is delivered to consumers in a reliable manner, power system planners and operators rely on computer-based modeling and analysis of the electric grid. The software currently being used for this purpose are designed to simulate either the high voltage transmission networks, or the low voltage distribution networks. Till now these software have worked well as the electricity flow in the electric grid is largely unidirectional, from the transmission network to the distribution network. Neglecting the distribution network topology in transmission network models or vice-versa in such a structure of the electric grid does not introduce significant calculation errors. However, the rapid growth of consumer-owned and operated solar photovoltaics (PV) based distributed generation over the last few years, which is expected to continue in the foreseeable future, has necessitated a rethink of this modeling and analysis paradigm. As the penetration levels of distributed generation increase, the electric grid will become increasingly decentralized and there will be bi-directional flow of electricity between the transmission and distribution networks. Accurate analysis of such a decentralized electric grid cannot be performed if either the distribution or the transmission network topology is neglected in the models. Integrated transmission and distribution system modeling and simulation, where transmission and distribution networks are modeled as one single unit, has, therefore, become an important research area in recent years. This dissertation makes a contribution to this research area by presenting an analysis approach and a program that can be used to simulate the dynamics (time varying behavior of the electric grid when subjected to disturbances such as short-circuits) of integrated transmission and distribution system models. A dynamic model of solar PV-based distributed generation that can be used to simulate their behavior during dynamic simulations is also investigated. Finally, an application of the program is discussed where the impact of solar PV-based distributed generation on the dynamics of the electric grid is studied by using the solar PV model and an integrated transmission and distribution system model. The dissertation shows that by simulating integrated transmission and distribution system models using the dynamic simulation program, insights about the impact of solar PV-based distributed generation on the dynamics of the electric grid can be obtained, which the transmission only models cannot provide.

Yes / This paper proposes a probabilistic multi-objective optimization method for the operation of three-phase distribution networks incorporating active network management (ANM) schemes including coordinated voltage control and adaptive power factor control. The proposed probabilistic method incorporates detailed modelling of three-phase distribution network components and considers different operational objectives. The method simultaneously minimizes the total energy losses of the lines from the point of view of distribution network operators (DNOs) and maximizes the energy generated by photovoltaic (PV) cells considering ANM schemes and network constraints. Uncertainties related to intermittent generation of PVs and load demands are modelled by probability density functions (PDFs). Monte Carlo simulation method is employed to use the generated PDFs. The problem is solved using ɛ-constraint approach and fuzzy satisfying method is used to select the best solution from the Pareto optimal set. The effectiveness of the proposed probabilistic method is demonstrated with IEEE 13- and 34- bus test feeders.

As society proceeds to advance and rely more and more on electricity, the number of high power solutions are on the rise for all types of industries. One industry where this is prolificis in the charging industry which has increasing demands coming from across the transport sector. In this thesis the objective is to unveil the growing need of high power charging solutions in electric aviation and how these implementations should be faced at an airport. Turnaround time for airplanes is crucial to keep low and with this comes the need of powerful electrical infrastructure. The increase of nationwide fast charging infrastructure and power-hungry industries takes a large toll on the regional and nationwide electrical grid. To analyze this problem in the thesis, a battery energy storage system and photovoltaic system are put into action and their impact is simulated in MATLABs Simulink. Results are gathered and the positive peak shaving capabilities for this potential solution is clear. Results also show a substantial increase to the total power consumed by the airport as fast charging is introduced for electrical airplanes. It also showed how well a battery energy storage system pairs with a photovoltaic system. Furthermore, the photovoltaic system works well with supplying both the airport infrastructure as well as the battery storage system with residual electricity. Another aspect of a photovoltaic system lies in its reflective properties, which can blind both pilots and other personnel. Therefore it is important that location for the photovoltaic system is well thought out to ensure safety at the airport. In future research a more detailed and complex simulation model would be recommended to have more reliable results.

Xanthomonas oryzae pv. oryzae (Xoo) est une bactérie phytopathogène responsable de la bactériose vasculaire du riz, maladie pouvant engendrer de fortes pertes de rendement à travers le monde. La course à l'armement entre la bactérie et sa plante hôte correspond d'une part à la mise en place de la virulence par le microorganisme et d'autre part en la résistance du végétal face à l'agression. Comprendre les mécanismes par lesquels Xoo accompli son cycle infectieux est d'une importance cruciale pour le développement futur de nouvelle méthode de luttes. Plusieurs approches complémentaires ont été mises en uvre afin de caractériser des éléments associés au pouvoir pathogène de Xoo.Dans un premier temps nous avons effectué une analyse protéomique comparative. Cette approche a permis l'identification chez une souche Africaine de Xoo d'un jeu de protéines induites par HrpX et susceptibles de jouer un rôle dans la virulence. Dans un second temps, l'implication de deux peptides dans la virulence Xoo a été étudiée. Le premier de ces peptides, supposé être le facteur d'avirulenceAvrXa21, a fait l'objet d'une caractérisation fonctionnelle et phylogénique. Le second peptide est synthétisé par un cluster NRPS, similaire à l'un de ceux présent chez Xanthomonas albilineans. Afin d'élucider l'importance de la molécule synthétisée par cette voie pour Xoo, une étude préliminaire impliquant la mutation d'un élément régulateur des NRPS a été effectuée. En dernier lieu, des informations nouvelles ont été apportées sur la topologie de la protéine membranaire HrcR qui est une composante essentielle du système de sécrétion de type III chez la plupart des bactéries appartenant au genre Xanthomonas. / Xanthomonas oryzae pv. oryzae (Xoo) is the agent of bacterial leaf blight BLB in rice, a disease which causes considerable yield losses throughout the world. In the arms race underlying the interactions between the microorganism and the host, the presence of virulence factors in the former parallels that of resistance factors in the latter. Understanding the mechanisms of Xoo's infectious cycle is of paramount importance for the elaboration of new fighting strategies to combat BLB. To achieve this, several complementary approaches to characterize components of Xoo's pathogenicity have been employed.First, we performed comparative proteomics that allowed us to identify novel HrpX-induced candidate pathogenicity factors of an African Xoo strain. Second, the involvement of two peptides in Xoo's pathogenicity has been investigated. One was speculated to be the avirulence factor AvrXa21 and has been characterized both functionally and phylogenetically. The other one was found to be synthesized by a Non-Ribosomal Peptide Synthetase (NRPS), reminescent to NRPS genes found in Xanthomonas albilineans. In order to determine the role of NRPS-mediated synthesis in Xoo virulence, we studied a strain carrying a mutated regulatory gene of the NRPS pathway. Finally, we provide new information on the topology of the HrcR membrane protein which is a conserved component of the type III secretion system of most Xanthomonas.

The thesis is divided into two main parts, theoretical and practical. It deals with the solution to assess the level of physical skills combined with preschool children, as a higher level of organized skiing helps training organized by kindergarten and what effect this can have skiing skills training on cognitive abilities and social side of the child's personality. The first section contains theoretical information drawn from the bibliographic sources of pedagogy, psychology, antropomotoriky, physical education and sport. Proceeds from the general content of the first chapter of her kindergarten educational concepts of the system, organizational forms of physical education and movement preschool child. It is followed by the second chapter a more specific topic for skiing children. Based on the development of motor skills through learning to its evaluation of motor skills on skis. Linkages between individual chapters see you in finding criteria for assessing the level of motor skills, cognitive skills and socialization in FEP PE curriculum. The practical part describes the action research group involved in preschool children with processed results of a detailed assessment of the level of motor skills on skis. At each level of subchapters is compared to skiing skills between age and sex of children,...

Cette thèse porte sur le développement d’une approche pratique de modélisation/simulation des systèmes solaires combinés Photovoltaïques/Thermiques PV/T. Il s’agit d’une approche basée sur un modèle d’ordre réduit en représentation d’état (ORRE). En effet, les systèmes solaires thermiques, électriques et combinés intégrés aux bâtiments possèdent des spécificités permettant de s’affranchir des méthodes numériques classiques (mécanique des fluides numérique et thermique numérique). Ces méthodes sont réputées dans le domaine de l’aérodynamique, de l’aéraulique…etc. Par contre, dans le domaine du mix-énergétique tels que celui considéré dans ce mémoire, l’application directe de ce modèle peut conduire à des dépassements des capacités mémoire ou des temps de calcul exorbitants. Une alternative est de développer des méthodes adaptées au problème physique considéré, en traitant l’aspect multi-physique toute en restant dans une taille de données raisonnable et du temps de calcul réduit. La méthodologie de modélisation consiste à réduire les dimensions des équations qui régissent le problème. En se basant sur la symétrie du système, puis en découpant le système en zones de contrôle basées sur une valeur moyenne gouvernée par les nombres adimensionnels de Biot (Bi) et de Fourier (Fo). Les résultats obtenus en fonctionnement dynamique pourront nous fournir des paramètres de sorties, plus particulièrement, les rendements électrique, thermique et la puissance de circulation du fluide caloporteur. L’avantage de l’approche proposée réside dans la simplification du modèle résultant, qui est représenté par un seul système d’équations algébriques en représentation d’état regroupant tous les éléments physiques du système en fonctionnement dynamique (conditions aux limites variables dans le temps). Ce modèle regroupe la variable fondamentale qui est la température, et les deux types de contrôle et de conception. De plus, le modèle d’ORRE est intégrable dans le fonctionnement en temps réel des systèmes PV/T intégrés aux bâtiments (PV/T-Bât) afin d’accompagner leurs régulation et gestion des flux mise en jeu. Le modèle ainsi proposé a fait l’objet d’une validation où les résultats numériques ont été comparés aux résultats expérimentaux. En effet, quatre configurations ont été étudiées et évoquées dans une approche linéaire. Les résultats obtenus montrent une cohérence tolérable entre les résultats expérimentaux, et numériques. Cette cohérence a été évaluée en termes d’incertitude entre les résultats du modèle et le cas étudié expérimentalement. Le cas d’un système non-linéaire a été également abordé. En effet, rares sont les travaux qui ont été publiés mettant en valeur les phénomènes non-linéaires dans les systèmes complexes PV/T-Bât, Ainsi, on a développé avec la même stratégie, des modèles bilinéaires qui modélise le mieux possible le comportement thermique dans les systèmes PV/T-Bât. Une étude d’optimisation du système multi-physique en introduisant une étude paramétrique est menée en terme afin d’étudier la sensibilité des paramètres sur le rendement énergétique. Cependant, les études d’optimisation paramétriques restent limitées et insuffisantes à cause de la résolution mono-objectif du problème d’optimisation, alors que notre système manifeste un comportement combiné et multi-physique de nature contradictoire. Pour ce faire, une optimisation multi-objectifs est introduite avec trois fonctions objectif en employant l’algorithme génétique NSGA-II. L’originalité de notre méthode est d’employer l’algorithme en régime dynamique afin de choisir la conception du système la plus optimale. Les résultats trouvés peuvent contribuer à améliorer la conception des systèmes PV/T-Bât et l’optimisation de leur fonctionnement / This thesis consists to develop a simplified model approach for Photovoltaic / Thermal (PV / T) combined solar system based on state-space reduced order model. The building integrated solar systems are getting high attention in these last decencies, as well as the performance increasing which require high numerical methods to improve the design and reducing the costs. In one hand, the CFD methods are useful tool to predict the energy (mechanical and thermal) of combined PV/T systems, but it requires an expensive computing capacities and exorbitant calculation times, On the other hand, the PV/T systems can generate both the electrical and thermal flows, and requires an easily and performant optimization model. An alternative is to develop methods that are adapted to the physical problem under consideration, treating the multi-physics aspect while remaining in a reasonable data size and reduced computing time. The first part of the current thesis consists to develop a mathematical model which consists of reducing the dimensions of the governed equations. Based on the symmetry of the geometry, the system is subdivided into control areas which governed by the dimensionless Biot (Bi) and Fourier (Fo) numbers. The obtained results in dynamic mode can provide output key parameters, more particularly the electrical and thermal efficiencies and the dissipated hydrodynamic power. The advantage of this approach lies in the simplification of the resulting model, which is represented by a single state-space representation that groups all the physical elements of the system into dynamic mode, i.e. in continuous variation of the boundary condition. This model groups the fundamental variable, which is the temperature, and two type parameters, which are the control parameters and the design parameters. In addition, the reduced order model can be integrated into real-time operation of building-integrated PV / T (BIPV/T) systems in order to support their regulation and management of intervening flows. In order to validate the use of our model, it is necessary to test it for several cases of Building Integrated PV/T systems (BIPV/T). For this, four major configurations were studied and discussed in a linear approach; the found results show a good agreement with experimental works. A second level has been developed as part of our thesis work, which is the non-linearity in combined PV / T and BIPV/T systems; in particular, bilinear models have been developed with the same strategy which best models the thermal behavior in BIPV/T systems. The second issue, related to Multi-physics aspect. Furthermore, in order to evaluate the sensitivity of the parameters, a parametric optimization has been made with dimensionless numbers. However, parametric optimization studies remain limited and insufficient because of the single-objective resolution of the optimization problem, whereas our system manifests a mixed and multi-physics behavior with contradictory nature. To do this, a multi-objective optimization is introduced with three objective functions using the NSGA-II genetic algorithm. The originality of our method is to use the algorithm in dynamic mode in order to choose the design of the optimal system. The found results can contribute to the design of BIPV/T systems and optimize their operation

Este trabalho propõe um sistema fotovoltaico (PV) multifuncional conectado a um sistema elétrico trifásico a quatro fios e operando com estratégia dual de compensação ativa de potência. Além da injeção de potência ativa na rede elétrica, o sistema PV operara como um condicionador unificado de qualidade de energia (UPQC) suprimindo as correntes harmônicas da carga e compensando a potência reativa. Adicionalmente, tensões balanceadas, reguladas e livre de harmônicos são fornecidas para a carga. O sistema PV-UPQC é baseado em uma estratégia de compensação dual, na qual o conversor série opera como uma fonte de corrente senoidal, enquanto o conversor paralelo atua como uma fonte de tensão senoidal. Como resultado, nenhum comportamento transitório é observado na troca entre os modos de operação ilhado e interconectado do sistema PV-UPQC. Além disso, para contornar os problemas associados às mudanças bruscas de irradiação solar, uma malha de controle feed-forward atua na geração das referências de corrente do conversor série, de modo que se obtenha um rápido balanço de potência entre o sistema PV e a rede elétrica. Como resultado, as respostas dinâmicas tanto da corrente elétrica quanto da tensão do barramento CC são melhoradas. A análise detalhada envolvendo o fluxo de potência ativa dos inversores é realizada proporcionando um melhor entendimento sobre a operação do sistema PV-UPQC, bem como auxiliando no dimensionamento dos conversores de potência. Resultados de simulação e experimentais são apresentados para avaliar os desempenhos estáticos e dinâmicos do PV-UPQC conectado ao sistema elétrico de distribuição. / This work proposes a multifunctional single-stage three-phase four-wire grid-connected photovoltaic (PV) system operating with a dual active power-line compensating strategy. Besides injection of active power into the grid, the PV system operates as a unified power quality conditioner (UPQC), suppressing the load harmonic currents and compensating reactive power. Additionally, regulated, balanced and harmonic free output voltages are provided to the load. Since the PV-UPQC is based on a dual compensation strategy, the series converter operates as a sinusoidal current source, whereas the parallel converter operates as a sinusoidal voltage source. Thus, seamless transition can be achieved from the interconnected to the islanding operation modes, and vice-versa, without load voltage transients. Moreover, to overcome problems associated with sudden solar irradiation changes, fast power balance involving the PV array and the grid is obtained, since the feed-forward control loop acts on the generation of the series inverter current references. As a result, the dynamic responses of both inverter currents and dc-bus voltage are improved. Detailed analysis involving the active power flow through the inverters is performed allowing proper understanding of the PV-UPQC operation, as well as assisting to design of the power converters. Simulation and experimental results are presented to evaluate both dynamic and static performances of the PV-UPQC tied to the electrical distribution system.

Genomas bacterianos são extremamente dinâmicos e boa parte dessa dinâmica ocorre devido a transferência horizontal e aquisição de DNA exógeno, um processo natural e fundamental para a evolução, adaptação e diversificação dos microrganismos. Ilhas genômicas são grandes regiões do cromossomo bacteriano adquiridas por transferência horizontal e estão presentes em apenas algumas linhagens, podendo conferir alguma vantagem adaptativa. Em Pseudomonas aeruginosa, até o momento foram caracterizadas pelo menos dezesseis ilhas genômicas e cada uma delas confere características diferentes a seus hospedeiros. Em P. aeruginosa UCBPP-PA14 (ou simplesmente PA14), encontram-se duas ilhas de patogenicidade denominadas PAPI-1 e PAPI-2, sendo a primeira a maior e a mais estudada, contendo 115 ORFs (“Open Reading Frame”, ou quadros abertos de leitura). Dentre estes, o gene int codifica uma integrase essencial para a excisão e integração de PAPI-1, e o gene soj é necessário para a manutenção da ilha na célula e é expresso apenas quando esta se encontra na forma epissomal. PAPI-1 codifica um provável sistema de secreção tipo IV (T4SS), similar ao do elemento móvel ICEHin1056, responsável pela transferência deste para outras bactérias. O objetivo deste trabalho foi identificar fatores genéticos e ambientais que contribuem para a transferência de PAPI-1 entre linhagens de P. aeruginosa. Foi observado que os mutantes por transposon nos genes PAPI-1 PA14_59860, PA14_59880, PA14_59920 e PA14_59940 têm a frequência de transferência de PAPI-1 diminuída, mas os genes interrompidos nesses mutantes não são essenciais para a excisão da ilha. Também foi mostrado que os reguladores percepção de quorum RhlR e MvfR têm influência na expressão do gene int, mas não de soj. O terceiro regulador de percepção de quorum, LasR, assim como a proteína H-NS MvaT, não tem influência na expressão de int e de soj. Ensaios de RT-PCR quantitativos mostraram que o choque térmico aumentou os níveis do mRNA de soj, mas não de int, corroborando dados previamente sugeridos pela literatura, que mostram uma maior freqüência de transferência de PAPI-1 nessas condições. Também foi analisado se o segundo mensageiro celular em bactérias, c-di-GMP, poderia contribuir para a excisão/manutenção de PAPI-1. Alterações nas concentrações deste segundo mensageiro pela superexpressão de proteínas responsáveis por sua síntese ou degradação não foram capazes de afetar a excisão/manutenção de PAPI-1. Houve diminuição na frequência de transferência de PAPI-1 a partir de linhagens doadoras superexpressando uma diguanilato ciclase ou uma fosfodiesterase de c-di-GMP, mas provavelmente este efeito não se deve aos níveis alterados desse segundo mensageiro. Em Xanthomonas axonopodis pv. citri 306 (XAC), uma região de 86 kb possui uma grande semelhança com PAPI-1 na organização dos genes. Além disso, possui uma série de ORFs relacionados aos genes “core” que definem uma família de ilhas genômicas sintênicas das quais PAPI-1 faz parte. Entretanto, os genes acessórios encontrados entre os blocos de genes conservados varia muito entre PAPI-1 e a região de XAC. Foi determinado que esta região de XAC não pode se excisar do cromossomo nas condições analisadas. Por fim, com o intuito de verificar as possíveis interações entre os produtos dos genes conservados em PAPI-1 e XAC, ensaios de duplo-híbrido foram realizados, porém não foi possível determiná-las, visto que apenas resultados falsos positivos foram obtidos. Este trabalho mostrou pela primeira vez que a percepção de quorum está envolvida com a expressão de soj e determinou uma extensa similaridade entre essa ilha e uma região do genoma de XAC / Bacterial genomes are extremely dynamic mostly because of horizontal gene transfer, a natural and fundamental process for evolution, adaptation and diversification of microorganisms. Genomic islands are large DNA segments acquired by horizontal gene transfer which are present only in a few strains and may confer some adaptative advantage. At least sixteen genomic islands have been characterized in Pseudomonas aeruginosa to date and each confers different characteristics to its host strain. P. aeruginosa UCBPP-PA14 (PA14) harbors two pathogenicity islands named PAPI-1 and PAPI-2. PAPI-1 is the largest one, carrying 115 open reading frames (ORFs). Among these, int codes for an integrase essential for PAPI-1 excision and integration, and soj is required for maintaining PAPI-1 in the cells, being expressed only when this island is in an episomal, circular form. PAPI-1 also harbors genes coding for a type four secretion system (T4SS) similar to the mobile element ICEHin1056, which is responsible for transferring this element to other bacteria. In this work, we show that transposon insertion in PAPI-1 genes PA14_59860, PA14_59880, PA14_59920 and PA14_59940 lowered the frequency of conjugation of PAPI-1 from PA14 to other bacteria, but those genes were not essential for PAPI-1 excision. Quorum sensing regulators RhlR and MvfR had a role in int expression, but did not alter soj transcription. The third quorum sensing regulator LasR, as well as the H-NS protein MvaT, did not alter both int and soj expression. Quantitative RT-PCR assays showed that cells incubated at heat shock conditions present higher levels of soj, mRNA, confirming published data that showed an increase in PAPI-1 transfer in these conditions. It was also analyzed whether the second messenger c-di-GMP would contribute to PAPI-1 excision/maintenance. Changes in the levels of this second messenger in cells overexpressing proteins responsible for its synthesis or degradation did not affect PAPI-1 excision and maintenance. A decrease in PAPI-1 transfer frequency was detected when those cells were used as donors in conjugation, but this effect cannot be attributed to the altered c-di-GMP levels. In Xanthomonas axonopodis pv. citri 306 (XAC), an 86 kb genome region shares similarity with PAPI-1 regarding gene homology and organization. It also carries ORFs related to the core genes that define a syntenic family of genomic islands that includes PAPI-1. Nevertheless, the accessory genes dispersed among the clusters of conserved genes are not related, when comparing PAPI-1 and this region in XAC. An epissomal form of this putative XAC island could not be detected in the conditions tested in this work. Finally, in order to verify interactions involving the conserved proteins in PAPI-1 and XAC, two hybrid assays were carried out, but only false positives results were obtained

Le cuivre, souvent utilisé pour gérer les bactérioses en agriculture, est largement utilisé dans la lutte contre Xanthomonas citri pv. citri (Xcc), agent responsable du chancre asiatique des agrumes. La récente détection d’un phénotype résistant au cuivre (CuR) chez Xcc dans deux territoires ultramarins français a motivé une étude génomique qui a révélé, dans les génomes de souches CuR, la présence d’un plasmide conjugatif portant un transposon adaptatif de type Tn3. Sa conservation chez plusieurs espèces de Xanthomonas phytopathogènes suggère le rôle des transferts horizontaux (HGT) dans l’adaptation de Xcc. Nous avons donc analysé, dans l’océan Indien, les relations phylogénétiques de souches sensibles et CuR en prenant en compte à la fois les SNP et les variations de contenu en gènes. La datation de la phylogénie a permis de formuler des scenarii d’introduction de la bactérie dans la région. La phylogénie a montré une structure géographique forte à l’échelle de l’océan Indien, qui s’estompe à l’échelle de la Réunion et disparaît à l’échelle du verger. Au sein des vergers, l’admixture est un élément favorable aux HGT entre souches génétiquement différentes. Ils sont pourtant peu caractérisés chez les bactéries du genre Xanthomonas. Nous avons ainsi analysé la dispersion de l’ensemble des gènes plasmidiques connus de la famille des Xanthomonadaceae dans l’ensemble des génomes bactériens de NCBI, mettant en évidence à la fois la forte prévalence des gènes plasmidiques au sein des Xanthomonadaceae mais aussi la limite taxonomique forte à leur échange par conjugaison. L’importance du mosaïsme plasmidique, en partie lié aux éléments mobiles a aussi été illustrée. L’ensemble de nos résultats souligne l’importance des HGT dans l’évolution des bactéries du genre Xanthomonas, et la nécessité de caractériser finement le contenu et le fonctionnement du génome environnemental des Xanthomonadaceae pour appréhender au mieux l’adaptation de ces bactéries phytopathogènes. / Copper, frequently used in agriculture to control bacterial diseases, is commonly used against Xanthomonas citri pv. citri (Xcc), the bacterial agent of Asiatic citrus canker. The recent detection of a copper-resistant phenotype in two French overseas regions motivated a genomic study which revealed, in copper-resistant (CuR) strains, a conjugative plasmid encoding an adaptive transposon of the Tn3 family. Its conservation in several Xanthomonas species suggested the role of horizontal gene transfer (HGT) in Xcc adaptation. We therefore analyzed the evolutionary history of susceptible and CuR Xcc strains in the Indian Ocean using both SNP and gene content variations. The dating of the obtained phylogeny allowed us to hypothesize the history of Xcc introduction into the region. The phylogeny showed a strong geographic structure among islands of the Indian Ocean region, which faded at the Réunion scale and disappeared at the grove scale. Among the groves, admixture is a factor favoring HGT between genetically distinct strains. This form of evolution is however largely uncharacterized in the Xanthomonas genus. To fill this gap, we searched genetic homology between the whole known plasmid gene content of the Xanthomonadaceae family and the complete set of genomes hosted in NCBI databases. We highlighted both the ubiquity of plasmid genes in the Xanthomonadaceae family and the taxonomical barrier of their sharing by conjugation. The small fraction of genes that were exchanged through the complete sharing of plasmids also revealed the importance of plasmid mosaicism, partly due to mobile genetic elements. Taken together, our results highlight the importance of bacterial communities in the evolution of phytopathogenic bacteria of the Xanthomonas genus, and the need for a precise characterization of the content and the functioning of the Xanthomonadaceae environmental genome in order to fully apprehend the adaptation of these phytopathogenic bacteria.