Orientadores: Pedro Duarte Novaes, Marcelo Rocha Marques / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-13T11:15:57Z (GMT). No. of bitstreams: 1 Vasconcelos_DanielFernandoPereira_D.pdf: 3147772 bytes, checksum: f14ddeae900959b567ea4b605c927b8b (MD5) Previous issue date: 2008 / Resumo: A administração intermitente de hormônio paratireóideo (PTH) é capaz de promover anabolismo ósseo, favorecendo a neoformação óssea em condições como osteoporose e reparo de fraturas. Sabendo-se que tanto os tecidos ósseos mandibulares e alveolares como os tecidos periodontais podem responder à administração de PTH de forma intermitente, o objetivo deste estudo foi avaliar histomorfometricamente o efeito da administração de PTH (1-34) em um modelo de defeito periodontal, do tipo fenestrado, na mandíbula de ratos. Foram utilizados neste estudo 32 ratos Wistar machos, com o objetivo de expor a região vestibular da raiz distal do primeiro molar inferior direito. A criação do defeito possibilitou a remoção parcial de ligamento periodontal e do cemento dentário. Após a cirurgia os animais foram separados aleatoriamente em 4 grupos (n=8): Grupo C14: administração intermitente (3 vezes por semana) do veículo de diluição do PTH por 14 dias; Grupo P14: administração intermitente de PTH (1-34) por 14 dias; Grupo C21: injeções intermitentes do veículo de diluição do PTH por 21 dias; Grupo P21: injeções intermitentes de PTH por 21 dias. Os animais foram mortos e preparados para avaliação histomorfométrica dos seguintes parâmetros: I - extensão inicial do defeito, II - extensão do defeito remanescente, III - área do defeito ósseo remanescente, IV - densidade do osso neoformado, V - área do calo formado e marcação para TRAP, VI - área do cemento neoformado, VII - análise do retardo óptico (birrefringência) do ligamento periodontal reinserido sobre a raiz operada. A análise intergrupo demonstrou que os defeitos tinham tamanhos similares inicialmente e que a administração de PTH reduziu significativamente (p<0,05) a extensão e a área do defeito remanescente. Além disso, o PTH aumentou significativamente (p<0,05) a densidade do osso neoformado, a área do calo, o número de osteoclastos presentes no calo formado, a área de cemento neoformado e a birrefringência do ligamento periodontal reinserido. Conclui-se que a administração intermitente de PTH (1-34), em modelo experimental de reparo em de ratos, pode influenciar positivamente o processo de reparação óssea e periodontal. / Abstract: The intermittent PTH administration is known to contribute to bone formation in cases of osteoporosis and bone fractures. Also, it has been reported to reduce periodontitis-related bone loss. The aim of this study was to evaluate the effect of anabolic PTH on periodontal repair and mandibular bone defect in rats. Fenestration defects were created unilaterally at the buccal surface of distal roots of lower first molars in Wistar rats (n=32), and both periodontal ligament and cementum were removed. Animals were then assigned to 4 groups (n=8): 1) C14 - placebo administration for 14 days; 2) P14 - PTH administration for 14 days; 3) C21 - placebo administration for 21 days; and 4) P21 - PTH administration for 21 days. All groups were treated intermittently (3 times a week). All animals were killed and prepared to histomorphometric analysis considering the following parameters: I - extension of the initial defect; II - extension of the remaining defect; III - area of the remaining defect; IV - density of neoformed bone; V - total callus area and staining for tartrate-resistant acidic phosphatase (TRAP); VI - area of the neoformed cementum; VII - polarized light microscopic analysis of root periodontal ligament reattachment. Intergroup analysis showed that the bone defects were initially similar in size (p>0.05). The intermittent PTH administration decreased (p<0.05) both extension and area of the remaining defect, and increased (p<0.05) the neoformed bone density and the total callus area. An increase in TRAP-positive cells was observed in the PTH treated groups (p<0.05). The area of the neoformed cementum and reattachment of the periodontal ligament were also observed to increase concerning PTH-treated groups (p<0.05). Data analysis suggests that the intermittent PTH administration might contribute to bone and periodontal repair in rats. / Doutorado / Histologia e Embriologia / Doutor em Biologia Buco-Dental

Orientador: Pedro Duarte Novaes / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-19T03:52:35Z (GMT). No. of bitstreams: 1 Omar_NadiaFayez_D.pdf: 1979982 bytes, checksum: 2ca79a3afb3b73396fe8807889683960 (MD5) Previous issue date: 2011 / Resumo: O ligamento periodontal (LP) é um tecido conjuntivo que ocupa o espaço entre o dente e seu alvéolo, e tem a função principal de ancoragem e suporte dos dentes. Baseados em dados na literatura que mostram que dentes em hipofunção são mais facilmente extraídos, investigamos qual(is) fatores e/ou moléculas poderiam ter relação com esse enfraquecimento da ancoragem do dente no osso por meio do LP. Dentre eles, investigamos a expressão de MMPs e TIMP-2, e organização do colágeno no LP, além da resistência que o LP oferece ao movimento eruptivo nos incisivos de ratos. Para isso, produzimos alteração na erupção de incisivos inferiores de ratos, durante períodos experimentais diferentes, produzindo hipofunção, hiperfunção e contenção do processo eruptivo, além da erupção normal; fazendo a medição da taxa de erupção em todos os grupos ao longo do período experimental. Os resultados mostraram que no grupo hipofuncional a atividade de MMP-2 e a desorganização do colágeno aumentaram, contribuindo para a diminuição da força de resistência ao movimento eruptivo e para o aumento da taxa de erupção neste grupo. No grupo contido, a erupção dos incisivos foi interrompida, porém a atividade de MMP-2 aumentou em níveis variados ao longo do período experimental, enquanto a organização do colágeno e, consequentemente, a força de resistência do LP ao movimento eruptivo foram levemente menores. Por outro lado no grupo hiperfuncional a taxa de erupção não sofreu alteração, mas a atividade de MMP-2 estava aumentada, juntamente com a força de resistência do LP ao movimento eruptivo, com leve desorganização do colágeno indicando, portanto, possíveis alterações em outras moléculas envolvidas no processo de remodelação da matriz extracelular no ligamento periodontal. As análises de MT1-MMP e TIMP-2, nas condições deste estudo, não mostraram diferenças estatísticas entre os grupos e períodos estudados. Esses dados sugerem papel da MMP-2 na remodelação do ligamento periodontal de incisivos de ratos, durante alterações na erupção, atuando diretamente sobre a degradação do colágeno, o que leva à alterações na resistência do ligamento ao movimento eruptivo, principalmente em hipofunção; e que MT1- MMP e TIMP-2 podem ter participação secundária neste processo / Abstract: The periodontal ligament (PL) is a tissue that occupies the space between tooth and its socket, and has main function to anchor and support the teeth. Based on published datas showing that hypofunctional teeth are more easily extracted, we investigated which one(s) factors and/or molecules could be related to the weakening of the periodontal ligament anchoring. We investigated the expression of MMPs and TIMP-2, collagen organization in the PL, and the resistance that the PL offers to the eruptive movement in rat incisors. Therefore, we altered the eruption of rat incisors, during different experimental periods, producing hypofunction, hyperfunction and restrain of the eruptive process, beyond the normal eruption, measuring the eruption rate, in all groups, throughout the experimental period. The results showed that in the hypofunctional group the MMP-2 activity and collagen disorganization increased, contributing to the decrease in the resistance strength to the eruptive movement and increased eruption rate in this group. In the restrain group, the incisor eruption was interrupted, but the MMP-2 activity increased, to varying degrees, throughout the experimental period, while the collagen organization and, thus, the resistance strength to the eruptive movement of the PL were slightly lower. On the other hand, in the hyperfunctional group the eruption rate did not change, but MMP-2 activity and PL resistance strength to eruptive movement were increased, with a slight collagen disorganization, indicating, therefore, possible changes in other molecules involved in the periodontal ligament extracellular matrix remodeling. Analyses of MT1-MMP and TIMP-2, under the conditions of this study, showed no statistical differences between groups and experimental periods. These data suggest the role of MMP-2 in periodontal ligament remodeling of rat incisors, during altered eruption, acting directly on collagen degradation, which leads to changes in PL resistance to the eruptive movement, in hypofunction condition, and MT1- MMP and TIMP-2 may have secondary participation in this process / Doutorado / Histologia e Embriologia / Doutor em Biologia Buco-Dental

Orientador: Pedro Duarte Novaes / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-22T06:39:35Z (GMT). No. of bitstreams: 1 Silva_KarlaRovarisda_M.pdf: 5383756 bytes, checksum: f46eb2dfd48068a21c19841f2e4d3581 (MD5) Previous issue date: 2013 / Resumo: Os efeitos adversos da radioterapia sobre os tecidos orais vêm sendo estudados com intuito de, cada vez mais, entender como esta age sobre o organismo, bem como para o desenvolvimento de métodos ou substâncias que visem à minimização dessas sequelas. Dentre os tecidos que estão na área de exposição, está o ligamento periodontal, que se afetado, pode ocasionar a perna dentária que impreterivelmente interfere na qualidade de vida do indivíduo. Este trabalho teve como objetivo avaliar o efeito da radiação ionizante sobre o ligamento periodontal do dente incisivo de rato albinus wistar, por meio da microscopia de polarização e do teste de força. A amostra constituiu-se de 30 ratos albinus wistar machos divididos em dois grupos, o grupo controle (15) e o grupo irradiado (15). O grupo irradiado foi submetido à sessão única de radioterapia com dose de 15Gy e após 14 dias todos os animais foram sacrificados. Um animal de cada grupo foi perdido durante a execução do trabalho. Desta forma, sete animais de cada grupo foram submetidos ao teste de resistência do ligamento periodontal e os sete restantes tiveram a organização do colágeno avaliada através da microscopia de polarização. Os resultados mostraram que ambos os testes apresentaram diferença estatisticamente significante entre os grupos (p<0,001). Através dos coeficientes de correlação de Pearson encontrou-se também uma forte correlação entre os resultados dos testes (resistência/polarização) de cada grupo (controle/irradiado) (R = 0,683; p < 0,001). Portanto, concluiu-se que a radioterapia pode levar à diminuição da resistência à força de intrusão e provocou a desorganização do colágeno no ligamento periodontal / Abstract: The adverse effects of radiotherapy on oral tissues have been studied in order to increasingly understand how it works on the body, as well as for the development of methods and substances aimed at minimizing these sequelae. Among the tissues that are in the exposition area, is the periodontal ligament which, when affected, can lead to tooth loss, that unfailingly interferes with quality of life. This study had as purpose to evaluate the effect of ionizing radiation over the periodontal ligament of the incisive tooth of the albinus wistar rat, trough the polarizing microscope and the strength test. The sample was composed of 30 albinus wistar male rats, separated into two groups, the control group (15) and the irradiated one (15). The irradiated group was subjected to an only session of radiotherapy, with a 15Gy dose, and after 14 days all the animals were sacrificed. One animal in each group was lost during the study's execution. Hence, seven animals of each group were subjected to the periodontal's ligament resistance test, and the seven remaining had their collagen organization evaluated trough the polarizing microscopy. The results showed that both tests exhibited statistically significance difference between the groups (p<0,001). Using the Pearson correlation coefficients a strong correlation was also found between the tests results (resistance/polarizing) of each group (control/irradiated) (R = 0,683; p<0,0001). Thereby, the conclusion was that radiotherapy can lead to a diminished resistance to the intrusion strength and to a disorganization of the periodontal's ligament collagen / Mestrado / Radiologia Odontologica / Mestra em Radiologia Odontológica

No description available.

The human jaw, like all other articulated body parts, exhibits small oscillatory movements during isometric holding tasks. These movements, known as physiological tremor, arise as a consequence of the interaction of various factors. One of these factors is reflex feedback from peripheral receptors. In the human jaw, receptors that innervate the periodontium are able to transduce minute changes in force. This thesis examines the contribution of these periodontal mechanoreceptors (PMRs) to the genesis of physiological tremor of the human jaw. By using frequency domain analysis of time series recorded during isometric biting tasks, the character of physiological jaw tremor can be revealed. Physiological jaw tremor was observed in force recorded from between the teeth as well as from electromyograms recorded from the principal muscles of mastication. These recordings have shown us that jaw physiological tremor consists of a frequency invariant component between 6 and 10Hz. This frequency remains unaltered under various load conditions where the mechanical resonance of the jaw would be expected to vary greatly (Chapter 2). Such findings indicate a ‘neurogenic’ origin for this tremor. A possible candidate for this neurogenic component of physiological tremor in the jaw is the reflex feedback arising from the PMRs. Using local anaesthetisation, it has been shown in this thesis, that by blocking outflow from the PMRs, the amplitude of neurogenic physiological jaw tremor can be reduced dramatically. This procedure caused a dramatic reduction in not only the mechanical recordings of tremor but also in the coupling between masseteric muscles bilaterally (Chapter 3) and between single motor units recorded from within a homonymous muscle (Chapter 4). The obvious mechanism by which periodontal mechanoreceptor anaesthetisation could reduce the amplitude of physiological tremor in the jaw would be by reducing the amplitude of the oscillatory input to the motoneurones driving the tremor. This interpretation remains controversial however as physiological tremor in the jaw can be observed at force levels above which the PMRs are supposedly saturated in their response. In light of this knowledge, the saturating characteristics of these receptors in terms of reflex output were examined. To do this, a novel stimulation paradigm was devised whereby the incisal teeth were mechanically stimulated with identical stimulus waveforms superimposed upon increasing tooth preloads. This necessitated the use of a frequency response method to quantify the reflexes. An optimal frequency for stimulation was identified and used to confirm that the hyperbolic saturating response of PMRs observed previously, translated to a similar phenomenon in masticatory reflexes (Chapter 5). These data reinforced the idea that physiological tremor in the jaw was not just a consequence of rhythmic reflex input from PMRs, as the dynamic reflex response uncoupled from the input as the receptor-mediated reflex response saturated. An alternative hypothesis was then developed that suggested the effect of PMR suppression in physiological tremor was via tonic rather than rhythmic effects on the masseteric motoneurone pool. By utilising a novel contraction strategy to manipulate the mean firing rate of the motor neuron pool at a given level of force production, data contained in Chapter 6 shows that population motor unit firing statistics influence the expression of physiological tremor, and such manipulations mimic, to an extent, the changes in firing statistics and tremor amplitude seen during anaesthetisation of the PMRs. This thesis therefore posits a mechanism whereby periodontal input influences the firing rate of motoneurones in such a way as to promote tremulous activity (Chapter 5). However, as this proposed mechanism did not explain the full extent of tremor suppression seen during PMR anaesthetisation it can therefore only be considered a contributing factor in a multifactor process. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1297555 / Thesis (Ph.D.) -- University of Adelaide, School of Molecular and Biomedical Science, 2007

The human jaw, like all other articulated body parts, exhibits small oscillatory movements during isometric holding tasks. These movements, known as physiological tremor, arise as a consequence of the interaction of various factors. One of these factors is reflex feedback from peripheral receptors. In the human jaw, receptors that innervate the periodontium are able to transduce minute changes in force. This thesis examines the contribution of these periodontal mechanoreceptors (PMRs) to the genesis of physiological tremor of the human jaw. By using frequency domain analysis of time series recorded during isometric biting tasks, the character of physiological jaw tremor can be revealed. Physiological jaw tremor was observed in force recorded from between the teeth as well as from electromyograms recorded from the principal muscles of mastication. These recordings have shown us that jaw physiological tremor consists of a frequency invariant component between 6 and 10Hz. This frequency remains unaltered under various load conditions where the mechanical resonance of the jaw would be expected to vary greatly (Chapter 2). Such findings indicate a ‘neurogenic’ origin for this tremor. A possible candidate for this neurogenic component of physiological tremor in the jaw is the reflex feedback arising from the PMRs. Using local anaesthetisation, it has been shown in this thesis, that by blocking outflow from the PMRs, the amplitude of neurogenic physiological jaw tremor can be reduced dramatically. This procedure caused a dramatic reduction in not only the mechanical recordings of tremor but also in the coupling between masseteric muscles bilaterally (Chapter 3) and between single motor units recorded from within a homonymous muscle (Chapter 4). The obvious mechanism by which periodontal mechanoreceptor anaesthetisation could reduce the amplitude of physiological tremor in the jaw would be by reducing the amplitude of the oscillatory input to the motoneurones driving the tremor. This interpretation remains controversial however as physiological tremor in the jaw can be observed at force levels above which the PMRs are supposedly saturated in their response. In light of this knowledge, the saturating characteristics of these receptors in terms of reflex output were examined. To do this, a novel stimulation paradigm was devised whereby the incisal teeth were mechanically stimulated with identical stimulus waveforms superimposed upon increasing tooth preloads. This necessitated the use of a frequency response method to quantify the reflexes. An optimal frequency for stimulation was identified and used to confirm that the hyperbolic saturating response of PMRs observed previously, translated to a similar phenomenon in masticatory reflexes (Chapter 5). These data reinforced the idea that physiological tremor in the jaw was not just a consequence of rhythmic reflex input from PMRs, as the dynamic reflex response uncoupled from the input as the receptor-mediated reflex response saturated. An alternative hypothesis was then developed that suggested the effect of PMR suppression in physiological tremor was via tonic rather than rhythmic effects on the masseteric motoneurone pool. By utilising a novel contraction strategy to manipulate the mean firing rate of the motor neuron pool at a given level of force production, data contained in Chapter 6 shows that population motor unit firing statistics influence the expression of physiological tremor, and such manipulations mimic, to an extent, the changes in firing statistics and tremor amplitude seen during anaesthetisation of the PMRs. This thesis therefore posits a mechanism whereby periodontal input influences the firing rate of motoneurones in such a way as to promote tremulous activity (Chapter 5). However, as this proposed mechanism did not explain the full extent of tremor suppression seen during PMR anaesthetisation it can therefore only be considered a contributing factor in a multifactor process. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1297555 / Thesis (Ph.D.) -- University of Adelaide, School of Molecular and Biomedical Science, 2007

Resumo: O prognóstico de um dente reimplantado está relacionado a integridade do ligamento periodontal cementário e da camada de cemento. Esta, por sua vez, e influenciada por vários fatores, sendo os principais, o periodo extra-alveolar e o meio de conservação. Existem dúvidas, no entanto, se alterações sistêmicas, como o diabetes, também podem interferir no prognóstico do reimplante dentário. Portanto, o propósito deste trabalho foi avaliar o processo de reparo do reimplante de incisivos de ratos diabéticos nao controlados após a manutenção dos dentes em leite bovino pasteurizado. Para tanto, 32 ratos foram divididos em dois grupos de 16 animais cada, sendo que no Grupo I foi administrada, por via endovenosa, injeção de tampão citrato e no Grupo II inoculada estreptozotocina dissolvida em tampão citrato pela mesma via, para a indução do diabetes. Após controle glicêmico e a comprovação das altas taxas de glicemia, o incisivo superior direito de cada animal foi extraído e mantido em leite durante sessenta minutos. Na sequência, realizou-se o tratamento endodontico com a colocação de curativo de hidróxido de cálcio e o posterior reimplante dos dentes em seus respectivos alveolos. A antibioticoterapia sistêmica foi instituida no pós-operatório imediato. Decorridos 10 e 60 dias após o reimplante, realizou-se a eutanasia dos animais e as peças obtidas foram submetidas ao processamento laboratorial para a obtenção dos cortes, os quais foram corados com hematoxilina e eosina e Tricromicro de Masson para análise histologica e histométrica. Os resultados demonstraram que o tecido conjuntivo adjacente a superfície radicular se apresentou desorganizado nos animais diabéticos. Ìndices menores de reabsorção radicular externa e anquilose também foram encontrados neste grupo, porém, sem diferenças estatísticas significantes. / Abstract: The prognosis of a replanted tooth is related to the integrity of the cemental periodontal ligament and the cementum layer, which, in turn, is influenced by several factors. Among these factors, the extra-alveolar time and the storage medium are the most important. However, it remains unclear whether systemic alterations, such as diabetes, may also interfere with the prognosis of tooth replantation. Therefore, the purpose of this investigation was therefore to evaluate the healing process of non-controlled diabetic rats' incisors replanted after storage of the teeth in bovine pasteurized milk. A total of 32 rats were assigned to two groups of 16 animals each. In group I, a citrate buffer injection was administered intravenously to rats while the animals in group II received an intravenous injection of streptozotocin dissolved in citrate buffer to induce diabetes. After confirmation of high glycemic levels, the maxillary right incisor of each animal was extracted and stored in milk during 60 minutes. Thereafter, the teeth were endodontically treated, with placement of a calcium hydroxide-based intracanal dressing, and the teeth replanted into their sockets. Systemic antibiotic therapy started in the immediate postoperative period. The animals were euthanized 10 and 60 days postoperatively. The anatomical pieces were removed, submitted to routine laboratorial processing and serially sectioned to obtain cuts that were stained by hematoxylin and eosin and Masson's Trichrome for histologic and histometric analyses. The results showed that the connective tissue adjacent to the root surface was disorganized in the diabetic rats. Lower indices of external root resorption and ankylosis were also observed in the diabetic group, though with no statistically significant difference. / Orientador: Celso Koogi Sonoda / Coorientador: Alessandra Marcondes Aranega / Banca: Luis Eduardo Marques Padovan / Banca: Wilson Roberto Poi / Mestre

O objetivo deste estudo foi avaliar os efeitos da glicose elevada sobre células do ligamento periodontal humano, e esclarecer o papel de microRNAs (miRNAs) nas alterações observadas. As células foram obtidas de dentes pré-molares de crianças/adolescentes e divididas em grupos: controle (5 mM de glicose) e glicose elevada (30 mM de glicose), mantidos por 7 dias. A glicose reduziu a adesão celular, reduziu o espraiamento e inibiu a migração celular, com redução da velocidade e direcionalidade. A glicose não alterou a proliferação celular, mas aumentou a morte por apoptose e a expressão de caspase 3. A expressão de miRNAs 29a e 29ax não foi modulada pela glicose, enquanto a de miRNAs 221 e 222 mostrou-se reduzida. A inibição de miR-221 e miR-222 reduziu a migração e aumentou a morte celular, enquanto a superexpressão destes dois miRs aumentou a migração celular e reduziu a morte por apoptose. Em conclusão, células do ligamento periodontal humano são sensíveis à elevada concentração de glicose em funções como migração e sobrevivência. / The objective of this study was to evaluate the effects of high glucose on human periodontal ligament cells, and to clarify the role of microRNAs (miRNAs) in these effects. The cells were obtained from children / adolescents premolar teeth and divided into groups: control (5 mM glucose) and high glucose (30 mM glucose), maintained for 7 days. Glucose reduced cell adhesion, reduced spreading and inhibited cell migration, with reduced speed and directionality. Glucose did not alter cell proliferation, but increased death by apoptosis and caspase 3 expression. Expression of 29a and 29ax miRNAs was not modulated by glucose, whereas miRNAs 221 and 222 were reduced. Inhibition of miR-221 and miR-222 reduced migration and increased cell death, while overexpression of these two miRs increased cell migration and reduced death by apoptosis. In conclusion, cells of the human periodontal ligament are sensitive to high glucose concentration in functions such as migration and survival.

A periodontite é uma doença inflamatória crônica, multifatorial, altamente prevalente na população que compromete os tecidos de suporte dos dentes gerando sequelas de difícil resolução, mesmo com as mais modernas técnicas regenerativas. A terapia tecidual parece ser uma alternativa promissora e as células indiferenciadas do ligamento periodontal (PDL) tem demonstrado grande potencial terapêutico. No entanto, o PDL necessita de estímulos adequados de biomodificadores para que a diferenciação ocorra de forma coordenada. As proteínas da matriz do esmalte (EMD) é um tipo de biomodificador que promove formação de novo cemento. Apesar de ser utilizada clinicamente, a sua associação com células frescas do PDL ainda não foi explorada. Este é um estudo da expressão gênica e proteica do PDL com finalidade de reaproveitamento do tecido recém-extraído, estimulado por EMD, tendo em vista a regeneração periodontal. Os resultados da expressão gênica apresentam VEGF (p=0.5194) com diferença entre medianas de -0.201 e FGF-2 (p = 0,0059) com diferença entre medianas de -0.4167. No estudo de citocinas, VEGF-A (p<0,0001) com diferença entre medianas de 60,93, enquanto VEGF-D (p=0.0049) com entre medianas de 2,45. Através dos resultados da expressão gênica baseada em FGF-2 e proteica baseada em VEGF-A, foi possível observar que as EMD modularam a resposta tecidual ex-vivo no período de 10 minutos tendo em vista o padrão angiogênico. Essa combinação poderá servir como uma proposta terapêutica, visando a aplicação clínica futura de tecidos comumente descartados após exodontia, como o PDL. / Periodontitis is a chronic inflammatory disease, multifactorial and highly prevalent in the world population that affects the teeth of the supporting tissues, generating sequels difficult to solve even with the most modern regenerative techniques. Tissue therapy appears to be a promising alternative and undifferentiated cells of the periodontal ligament (PDL) have shown great therapeutic potential. However, the PDL needs to appropriate stimuli biomodificatores that differentiation occurs in a coordinated fashion. The enamel matrix proteins (EMP) are a type of biomodificator that promotes new cementum formation. Despite being used clinically, its association with PDL fresh cells has not yet been explored. This is a study of gene and protein expression of PDL with reuse purpose of the newly extracted tissue stimulated by EMD, with a view to periodontal regeneration. The results show VEGF gene expression (p = 0.5194) difference in median -001 and FGF-2 (p = 0.0059) difference in medians of -0.4167. In the study of cytokines, VEGF-A (p <0.0001) with the difference between medians of 60.93, whereas VEGF-D (p = 0.0049) with from 2.45 medians. Through the results of the FGF-2-based gene expression and protein-based VEGF-A, it was observed that the EMD modulated the tissue response ex vivo in the 10-minute period considertingthe standard angiogenic. This combination may serve as a therapeutic approach aimed at future clinical application of tissues commonly discarded after extraction, such as the PDL.

A periodontite é uma doença inflamatória crônica, multifatorial, altamente prevalente na população que compromete os tecidos de suporte dos dentes gerando sequelas de difícil resolução, mesmo com as mais modernas técnicas regenerativas. A terapia tecidual parece ser uma alternativa promissora e as células indiferenciadas do ligamento periodontal (PDL) tem demonstrado grande potencial terapêutico. No entanto, o PDL necessita de estímulos adequados de biomodificadores para que a diferenciação ocorra de forma coordenada. As proteínas da matriz do esmalte (EMD) é um tipo de biomodificador que promove formação de novo cemento. Apesar de ser utilizada clinicamente, a sua associação com células frescas do PDL ainda não foi explorada. Este é um estudo da expressão gênica e proteica do PDL com finalidade de reaproveitamento do tecido recém-extraído, estimulado por EMD, tendo em vista a regeneração periodontal. Os resultados da expressão gênica apresentam VEGF (p=0.5194) com diferença entre medianas de -0.201 e FGF-2 (p = 0,0059) com diferença entre medianas de -0.4167. No estudo de citocinas, VEGF-A (p<0,0001) com diferença entre medianas de 60,93, enquanto VEGF-D (p=0.0049) com entre medianas de 2,45. Através dos resultados da expressão gênica baseada em FGF-2 e proteica baseada em VEGF-A, foi possível observar que as EMD modularam a resposta tecidual ex-vivo no período de 10 minutos tendo em vista o padrão angiogênico. Essa combinação poderá servir como uma proposta terapêutica, visando a aplicação clínica futura de tecidos comumente descartados após exodontia, como o PDL. / Periodontitis is a chronic inflammatory disease, multifactorial and highly prevalent in the world population that affects the teeth of the supporting tissues, generating sequels difficult to solve even with the most modern regenerative techniques. Tissue therapy appears to be a promising alternative and undifferentiated cells of the periodontal ligament (PDL) have shown great therapeutic potential. However, the PDL needs to appropriate stimuli biomodificatores that differentiation occurs in a coordinated fashion. The enamel matrix proteins (EMP) are a type of biomodificator that promotes new cementum formation. Despite being used clinically, its association with PDL fresh cells has not yet been explored. This is a study of gene and protein expression of PDL with reuse purpose of the newly extracted tissue stimulated by EMD, with a view to periodontal regeneration. The results show VEGF gene expression (p = 0.5194) difference in median -001 and FGF-2 (p = 0.0059) difference in medians of -0.4167. In the study of cytokines, VEGF-A (p <0.0001) with the difference between medians of 60.93, whereas VEGF-D (p = 0.0049) with from 2.45 medians. Through the results of the FGF-2-based gene expression and protein-based VEGF-A, it was observed that the EMD modulated the tissue response ex vivo in the 10-minute period considertingthe standard angiogenic. This combination may serve as a therapeutic approach aimed at future clinical application of tissues commonly discarded after extraction, such as the PDL.