Im Vortrag werden die Funktionsprinzipien von PGP sowie verwendete Verfahren dargelegt. Ziel ist die Schaffung einer geeigneten Technologie im Universitaetsrechenzentrum zur Sicherung des Mailbetriebs.

Magistrinio darbo pavadinimas „Vaikų hiperkinezinių sutrikimų šiuolaikinė slauga” Darbo vadovė: VU MF Psichiatrijos klinikos Vaikų psichiatrijos ir socialinės pediatrijos centro docentė Sigita Lesinskienė Darbo autorė: VU MF slaugos magistartūros II kurso S. Bakutytė Vilniaus Universitetas Medicinos Fakultetas, Vidaus ligų pagrindų ir slaugos katedra Darbo metodika. Tyrime dalyvavo 40 vaikų tėvų, kurių vaikai ambulatoriškai gydosi paskirtais psichostimuliatorių grupės preparatais (PGP) (Ritalin'u ® ar Concerta XL®). Atlikta pusiau struktūrizuota tėvų apklausa telefonu VUVL filialo Vaiko raidos centro Vaikų psichiatrijos skyriuje gydytų vaikų, kuriems diagnozuotas HS ir pradėtas gydymas psichostimuliatorių grupės preparatais (PGP). Anketa sudaryta iš dviejų dalių: pirma anketos dalis susideda iš 32 klausimų, kuri buvo sudaryta savarankiškai, ir antra anketos dalis – vaistų šalutinių poveikių vertinimo skalė (Russel A. Barkley and Kevin Murphy „SIDE EFFECTS RATING SCALE“). Duomenys apdoroti statistikos programa SPSS for Windows v. 9 bei “MS Exel 2002” programa. Darbo rezultatai. Daugiausia tyrime dalyvavo 11-15 m. amžiaus vaikų, kurie sudarė 75% visų tiriamųjų, t.y. 30 iš 40 vaikų. Berniukams HS diagnozuojamas ir gydymas skiriamas dažniau nei mergaitėms, tai sudarė atitinkamai 87,5% ir 12,5%. Vaikai stacionare pradėti gydyti psichostimuliatorių grupės preparatais (PGP): Ritalin‘u® ir Concerta XL® atitinkamai (23 berniukai - 57,5% ir 17 mergaičių – 42,5%). Du trečdaliai vaikų po... [toliau žr. visą tekstą] / SUMMARY Master‘s degree: Contemprorary nursing of children with hyperactive disorder Work tutor: VU MF Psichiatrijos klinikos Vaikų psichiatrijos ir socialinės pediatrijos centro docentė Sigita Lesinskienė Work author: student of the II course of master nursing speciality, Sandra Bakutytė Methodology. In this research participate 40 child parents, who children dispensary take psycho-stimulants group preparations (Ritalin or Concerta). This research accomplished parents interview by telephone, who children diagnosed ADHD and medicated psycho-stimulants group preparations in Child evolution centre children psychiatrical department of VUVL affiliate. Questionnaire made of two parts: first questionnaire part consist of 32 questions, which made independently, and second questionnaire part – medicine side effects rating scale (Russel A. Barkley and Kevin Murphy “Side effects rating scale”). Information processed by statistical programme SPSS for Windows v. 9 and “MS Exel 2002”. Results. The most part in the research participate 11 – 15 years old child, which consist 75%, it is 30 of 40 children. ADHD for boys is diagnosing and healing often than girls, it takes accordingly 87,5% and 12,5%. Children in stationary medicated psycho-stimulants group preparations: Ritalin and concerta accordingly 23 boys – 57,5% and 17 girls – 42,5%. Two-thirds of child after medical continues psycho-stimulants usage dispensary, but more than half 25 (62,5%) of all child medicines takes not regular, it... [to full text]

Stuttgart, Univ., Diplomarb., 2001.

Trichoderma species are ubiquitous soil saprotrophs and well-characterized biological control agents. Certain strains have also been shown to stimulate plant growth through the production of bioactive secondary metabolites, and are therefore receiving increased attention as natural plant growth stimulants. Previous research at the University of Exeter (Ryder et al. (2012) Microbiology 158, 84-97) has shown that the Plant Growth Promotion activity of a biocontrol strain of Trichoderma hamatum (strain GD12) can be dramatically enhanced by targeted mutation of the N-acetyl-β-D-glucosaminidase-encoding gene nag. However, due to the loss in chitinase activities, the mutant (ΔThnag::hph) displays loss of saprotrophic competitiveness and reduced fitness as a biocontrol agent. We set out to investigate how we can use genetic manipulation to improve strain GD12 in the context of biological control and plant growth promotion. We approached this by firstly sequencing the whole genome of GD12 and then using the information available from this to produce a targeted deletion mutant in the GD12 background disrupting one of the most down regulated proteins in the ΔThnag::hph, a branched chain amino acid transaminase (bcat), implicated in the production of secondary metabolites. Secondly, we aimed to engineer hyper-secretion and enhanced PGP activities in GD12 without impairing biocontrol activity. Over-expression of the S. cerevisiae gene dolichol-phosphate mannose synthase (dpm1) in T. reesei leads to altered cell wall architecture and increased secretory potential. Using the constitutive promoter ToxA, we over-expressed the dpm1 gene in T. hamatum GD12 and assessed its effects on the biocontrol and PGP activities of the fungus. The data presented herein, shows, that bcat deletion in T. hamatum GD12 results in a detrimental effect of germination of lettuce seedlings grown in the presence of ∆Thbcat::hph. We show that single copy insertions of ToxA-dpm1 leads to improved PGP activities, while biocontrol fitness is unaffected. However, while multiple copy insertions similarly lead to enhanced PGP, such strains display impaired biocontrol of soil-borne pathogens such as the plurivorous damping-off pathogen Sclerotinia sclerotiorum. This work demonstrates that while significant improvements in crop productivity can be achieved through genetic modification of the beneficial rhizosphere fungus T. hamatum GD12, it can have important consequences for other aspects of its biology and ecology and competence as a soil-borne microorganism.

<p>Korisni mikroorganizmi ili mikroorganizmi promotori biljnog rasta (PGPM) ispoljavaju efekat na rast i razviće biljaka azotofiksacijom, mobilizacijom hraniva (N, P, Fe) u zemlji&scaron;tu i produkcijom fitohormona te na taj način stimuli&scaron;u rast biljaka, povećavaju prinos i &scaron;tite biljke od patogena. Cilj ovih istraživanja bio je da se izvr&scaron;i karakterizacija bakterija izolovanih iz rizosfere kukuruza, inokulacija semena kukuruza odabranim izolatima, ispitivanje efektivnosti primene pojedinačnih i združenih kultura izolata na mikrobiolo&scaron;ku aktivnost u rizosfernom zemlji&scaron;tu, kao i na početni rast i razvoj biljke, sadržaj pojedinih hranljivih elemenata u biljnom materijalu i prinos kukuruza. Kori&scaron;ćeno je 50 izolata iz rizosfernog zemlji&scaron;ta hibrida kukuruza NS 6010 gajenog na zemlji&scaron;tu tipa karbonatni černozem (13 izolata roda Azotobacter, 16 izolata roda Bacillus, 15 izolata roda Pseudomonas, 6 izolata roda Streptomyces). Karakterizacija izolata obuhvatila je određivanje morfolo&scaron;kih, fiziolo&scaron;kih, biohemijskih i PGP osobina. Inokulacija semena kukuruza izvr&scaron;ena je s osam odabranih izolata primenjenih pojedinačno i u sme&scaron;i i to s tri izolata azotobaktera (Azb5, Azb8, Azb13), s dva izolata bacilusa (Bac9, Bac15 ), s dva izolata pseudomonasa (Pse1, Pse5) i s jednim izolatom aktinomiceta (Act6). U ogledima su kori&scaron;ćeni hibridi kukuruza NS 6010 i NS 6030 selekcionisani u Odeljenju za kukuruz Instituta za ratarstvo i povrtarstvo, a efektivnost primene odabranih izolata na mikrobiolo&scaron;ku aktivnost u rizosfernom zemlji&scaron;tu, kao i na biljku kukuruza, ispitana je u polukontrolisanim i poljskim uslovima. Brojnost mikroorganizama određena je metodom agarnih ploča, a dehidrogenazna aktivnost spektrofotometrijskom metodom. Uticaj izolata na klijanje semena ispitan je u laboratorijskim uslovima. Uticaj izolata na početni rast<br />biljaka ispitan je u fazama 3. i 5-7. lista merenjem visine i suve mase nadzemnog dela<br />biljke. Sadržaj azota, fosfora, bakra i cinka u biljnom materijalu (nadzemni deo/list)<br />određen je u fazi 5-7. lista kukuruza za ogled u polukontrolisanim uslovima i u fazi svilanja<br />za ogled u polju. Ukupan prinos zrna kukuruza po jedinici povr&scaron;ine određen je<br />preračunavanjem prinosa po svakoj elementarnoj parceli. Najbolji efekat na brojnost<br />mikroorganizama dobijen je primenom izolata A zb5, B ac15, P se1, A ct6 i sme&scaron;e i zolata.Na povećanje dehidrogenazne aktivnosti najvi&scaron;e je uticala primena izolata Azb5, Azb8,<br />Bac15 i Act6. Inokulacija je imala pozitivan uticaj na klijavost, visinu i suvu masu<br />nadzemnog dela biljke, sadržaj N, P, Zn i Cu u biljnom materijalu, kao i prinos kukuruza.<br />Najbolji efekat na visinu i masu nadzemnog dela biljke u polukontrolisanim uslovima<br />utvrđen je sa izolatima azotobaktera, Bac15 i Pse1, dok je u poljskim uslovima najveće<br />povećanje ovih parametara dobijeno primenom izolata Pse5 i Act6. U proseku, najbolji<br />efekat na prinos dobijen je primenom sme&scaron;e izolata. Rezultati ovih istraživanja treba da<br />posluže kao osnova za dalja istraživanja koja će omogućiti odabir visoko kompatibilne<br />zajednice ispitivanih hibrida kukuruza i najefektivnijih vrsta PGPM u cilju proizvodnje<br />mikrobiolo&scaron;kog preparata kojim bi se ostvario veći prinos i omogućilo održavanje i<br />povećanje plodnosti zemlji&scaron;ta.<br />&nbsp;</p> / <p>Beneficial or plant growth promoting microorganisms (PGPM) exert effect on plant growth and development through nitrogen fixation, mobilization of nutrients (N, P, Fe) in soil and production of phytohormones and thereby stimulate plant growth, increase yield and reduce pathogen infection. The aim of this study was to perform characterization of bacteria isolated from maize rhizosphere, inoculation of maize seeds with selected isolates, testing the effectiveness of single and co-inoculation on microbial activity in rhizosphere, as well as on initial growth and development of plants, content of some nutrients in plant material and yield of maize. 50 isolates from rhizosphere of maize hybrid NS 6010 grown on calcareous chernozem soil (13 isolates of the genus Azotobacter, 16 isolates of Bacillus, 15 isolates of Pseudomonas, 6 isolates of Streptomyces) were used. Characterization of isolates included determination of morphological, physiological, biochemical and PGP traits. Inoculation of maize seeds was carried out with eight selected strains applied individually and in mixture, and that with three isolates of Azotobacter (Azb5, Azb8, Azb13), two isolates of Bacillus (Bac9, Bac15), two isolates of Pseudomonas (Pse1, Pse5) and one isolate of Streptomyces (Act6). The experimental objects were maize hybrids NS 6010 and NS 6030 developed at Maize Department, Institute of Field and Vegetable Crops, and the effectiveness of selected isolates on microbial activity in rhizosphere, as well as on maize plant, was tested in semi-controlled and field conditions. The number of microorganisms was determined by agar plates method, and dehydrogenase activity by spectrophotometric method. Effect of isolates on maize seed germination was tested in the laboratory. The effect of isolates on initial plant growth was tested at 3 and 5-7 leaf stages by measuring height and dry weight of above ground plant parts. The contents of nitrogen, phosphorus, copper and zinc in plant material (above ground part/leaf) were determined at 5-7 leaf stage for experiment in semi-controlled conditions and at silking stage for field trial. Thetotal grain yield per unit area is determined by recalculating yield for each elementary</p><p>plot. The best effect on the number of microorganisms was obtained by applying Azb5,<br />Bac15, Pse1, Act6 and mixtures of isolates. The largest increase in dehydrogenase activity<br />was achieved with isolates Azb5, Azb8, Bac15 and Act6. Inoculation had a positive impact<br />on germination, height and dry weight of above ground part of the plant, the content of<br />N, P , Z n and C u i n p lant material, as well as the yield of maize. The best effect on the<br />height and weight of above ground part of the plant in semi-controlled conditions was<br />achieved with isolates of azotobacters, Bac15 and Pse1, while in field conditions the<br />largest increase in these parameters was obtained with isolates Pse5 and Act6. On average,<br />the best effect on yield was obtained by using a mixture of isolates. The results of this<br />study should be the basis for further research which would allow the selection of highly<br />compatible community of maize hybrids and the most effective strains of PGPM and be<br />used in the production of microbial preparations in order to achieve higher yields and<br />facilitate the maintenance and increase fertility of soil.<br />&nbsp;</p>

<p>PGP mikroorganizmi (Plant Growth Promoting) su predmet mnogobrojnih istraživanja, čiji je glavni cilj pronalaženje adekvatnog načina njihove primene u poljoprivredi, hortikulturi, &scaron;umarstvu i za&scaron;tititi životne sredine. Cilj ovih istraživanja bio je izolacija i karakterizacija mikroorganizama sa PGP svojstvima iz rizosfere engleskog ljulja, ispitivanje sposobnosti njihovog preživljavanja nakon uno&scaron;enja u zemlji&scaron;te, kao i praćenje uticaja njihove primene na parametre prinosa i mikrobiolo&scaron;ku aktivnost u rizosferi biljke. Karakterizacija izolata roda Pseudomonas, Bacillus i Streptomyces obuhvatila je određivanje fiziolo&scaron;kih, biohemijskih i PGP osobina. Uticaj introdukcije odabranih izolata i gljive Trichoderma asperellum na brojnost i mikrobiolo&scaron;ku aktivnost u rizosferi engleskog ljulja, određivan je standardnim metodama na selektivnim hranljivim podlogama, a dehidrogenazna aktivnost spektrofotometrijskom metodom. U laboratorijskim uslovima ispitivan je efekat primene izolata na klijavost, dužinu korenka i stabaoca klice semena engleskog ljulja. U toku godine, uzimana su tri otkosa i pri tome su određivani dužina nadzemnog dela i korena biljke (cm) i prinos zelene i suve materije nadzemnog dela biljke po otkosu (t/ha). Na osnovu morfolo&scaron;kih, fiziolo&scaron;ko-biohemijskih, kao i PGP osobina koje su izolati pokazali, te na osnovu rezultata mnogobrojnih dosada&scaron;njih istraživanja, može se zaključiti da izolati P1 i P9 pripadaju vrsti Pseudomonas putida, izolat P12 Pseudomonas fluorescens, izolati B1, B3 i B6 vrsti Bacillus subtilis, a izolati A1, A2, A3 rodu Streptomyces. Brojnost pojedinih sistematskih i fiziolo&scaron;kih grupa mikroorganizama kao i enzimatska aktivnost u rizosferi engleskog ljulja, zavisila je od primenjenih inokulanata. Primena izolata Pseudomonas sp. P12 pozitivno je uticala na povećanje ukupnog broja mikroorganizama, brojnost gljiva, aminoheterotrofa i aktinomiceta. Izolat Bacillus sp. B1 uticao je na povećanje ukupnog broja mikroorganizama, brojnosti gljiva i azotobaktera. Primena izolata Streptomyces sp. A3 dovela je do povećanja broja aktinomiceta i oligonitrofila, dok je primena Trichoderma asperellum uticala pozitivno na povećanje broja aminoheterotrofa i azotobaktera. Primena izolata Pseudomonas sp. P12 i Streptomyces sp. A3 imala je najveći efekat na dehidrogenaznu aktivnost. Inokulacija je imala pozitivan uticaj na klijavost, svežu i suvu masu biljke, visinu i dužinu korena biljaka. Primena gljive Trichoderma asperellum i izolata Streptomyces sp. A3 delovala je pozitivno na klijavost, dužinu korenka i stabaoca klice. U proseku, najbolji efekat na prinos sveže i suve materije, kao i na visinu nadzemnog dela i dužinu korena biljke, imala je primena izolata Pseudomonas sp.P12 i Bacillus sp. B1.U proizvodnji krmnih trava mikroorganizmi jo&scaron; nisu na&scaron;li značajniju praktičnu primenu iako je veći i kvalitetniji prinos jedan od ciljeva stočarske proizvodnje. Rezultati ovih istraživanja su pokazali da se primenom mikroorganizama mogu postići pozitivni efekti i u proizvodnji engleskog ljulja. Zbog toga je veoma značajno da se vr&scaron;e dalja ispitivanja uzajamnog odnosa primenjenih mikroorganizama i engleskog ljulja u poljskim uslovima, kako bi se optimizirao način i vreme primene inokulanata</p> / <p>PGP microorganisms (Plant Growth Promoting) have been the subject of many research projects, whose main goal is to find appropriate methods of their use in agriculture, horticulture, forestry and environmental protection. The aim of this study is the isolation and characterization of microorganisms with PGP characteristics from the rhizosphere of perennial ryegrass, testing their ability to survive after entering the soil, and monitoring the impact of their application on yield parameters and microbial activity in the rhizosphere of plants. Characterization of Pseudomonas, Bacillus and Streptomyces included determination of the physiological, biochemical and PGP characteristics. Impact of the introduction of selected isolates and fungi Trichoderma asperellum on quantity and microbial activity in the rhizosphere of ryegrass was determined by using the standard method of selective media; dehydrogenase activity by the spectrophotometric method. The effects of the implementation of isolates on germination, seedling length sprouts seeds of perennial ryegrass were studied under laboratory conditions. During the year, three cuttings were taken. At each of the three cuttings, length of stem and roots of plants (cm) and yields of fresh and dry matter of the plant (t / ha) were measured.<br />Based on morphological, physiological and biochemical characteristics and PGP characteristics observed in the isolates, and based on the results of many previous studies, it can be concluded that (1) P1 and P9 isolates belong to the species Pseudomonas putida, (2)Pseudomonas fluorescens P12 isolate, isolates of B1, B3, B6, belong to the species Bacillus subtilis, and (3) A1, A2 and A3 isolates belong to the ordo Streptomyces. The quantity of systematic and physiological groups of microorganisms and enzymatic activity in the rhizosphere of ryegrass depended on the applied inoculants. Application of Pseudomonas sp. P12 had positive effects on increasing the total number of microorganisms, fungi, aminoheterotrophs and actinomycetes. Isolate Bacillus sp. B1 affected the increase of the total number of micro-organisms in the fungi and Azotobacter. Application of Streptomyces sp. isolates A3 led to an increase in the number of actinomycetes and oligonitrophyls, while the application of Trichoderma asperellum had positive impact on increasing the number aminoheterotrophs and Azotobacter. Application of Pseudomonas sp. P12 and Streptomyces sp. A3 had the greatest effect on dehydrogenase activity. Inoculation had a positive effect on germination, fresh and dry weight of plant, height and rootlength of plants. Introduction of fungus Trichoderma asperellum and Streptomyces sp. A3 isolate acted positively on germination, length of seedling of germs. On average, the best effects on the yield of fresh and dry matter, the height of the stem of the plant and the length of the root were attained by the application of Pseudomonas sp.P12 and Bacillus sp. B1 isolates. Microorganisms have not yet found significant practical use in the production of forage grasses, even though more qualitative yield has been sought in livestock production. The results of this study demonstrate that positive results in the production of perennial ryegrass can be achieved by the use of microorganisms. Therefore, it is very important to carry out further studies of the relationship between applied microorganisms and ryegrass under field conditions in order to optimize the method and time of application of inoculants.</p>

Bakgrund: Graviditetsrelaterad bäckensmärta (PGP) är vanligt förekommande hos gravida. Punktprevalensen har uppgetts till cirka 20 %. Bäckensmärtan utlöses av belastning och inverkar negativt på det dagliga livets aktiviteter såsom att bära, lyfta och förflytta sig. Det finns få studier på effekten av hållningskorrigering och ergonomi vid PGP. Syfte: Syftet med studien var att undersöka om en patientutbildning i ergonomi med fokus på praktisk träning av aktiviteterna gång, vändning i säng och uppresning hade effekt på smärta och aktivitetsbegränsning hos gravida kvinnor med bäckensmärta. Metod: En single-subject experimental design studie med AB design genomfördes med sex gravida deltagare med PGP. Interventionen bestod av praktisk träning av rörelsetekniker vid gång, vändning i säng och uppresning i kombination med information om PGP. Som utfallsmått för smärta användes Numeric Rating Scale, för aktivitetsbegränsning användes Timed Up and Go samt Pelvic Girdle Questionnaire. Två standardavvikelser-metoden (2SD-metoden) användes för att analysera data. Resultat: En deltagare upplevde sig helt återställd vid studiens slut och hade signifikant minskad smärta vid gång och vändning i säng samt signifikant minskad aktivitetsbegränsning. En deltagare upplevde sig mycket försämrad och hade signifikant ökad smärta vid uppresning samt signifikant ökad aktivitetsbegränsning. Övriga deltagare hade överlag oförändrade resultat vad gäller smärta och aktivitetsbegränsning. Slutsats: Det kunde inte ses någon positiv effekt på smärta och aktivitetsbegränsning förutom för en deltagare. Fyra av deltagarna upplevde en förbättring av sina bäckenbesvär vid studiens slut. / Background: Pregnancy related Pelvic Girdle Pain (PGP) is common. The point prevalence has been estimated to 20 %. The pain is elicited by movement and loading activities and is negatively affecting the activities of daily life. Few studies are made on the effect of correction of posture and ergonomics for PGP. Aim: The aim of the study was to evaluate possible changes in pain and disability after a treatment including patient education about ergonomics and practical training of a movement technique during walking, turning over in bed and rising from a chair. Method: A single-subject experimental design with AB-design was carried out. Six pregnant subjects with PGP were included. The subjects were educated about PGP and movement techniques during walking, turning over in bed and rising from a chair was taught and practiced. As outcome measures Numerical Rating Scale was used for pain, and Timed Up and Go and Pelvic Girdle Questionnaire were used for disability. The two standard deviation band method (2SD-method) was used to analyze data. Results: One subject got significantly reduced pain while walking and turning over in bed and got significantly reduced disability. One subject got significantly increased pain while rising from a chair and significantly increased disability. The majority of subjects got neither increased or decreased pain or disability. Conclusion: No positive effect on pain and disability could be established for more than one subject. Four subjects experienced their pelvic condition improved.

<p>The work in this thesis addresses design and development of multimodal dialogue recommender systems for the home context-of-use. In the design part, two investigations on multimodal recommendation dialogue interaction in the home context are reported on. The first study gives implications for the design of dialogue system interaction including personalization and a three-entity multimodal interaction model accommodating dialogue feedback in order to make the interaction more efficient and successful. In the second study a dialogue corpus of movie recommendation dialogues is collected and analyzed, providing a characterization of such dialogues. We identify three initiative types that need to be addressed in a recommender dialogue system implementation: system-driven preference requests, userdriven information requests, and preference volunteering. Through the process of dialogue distilling, a dialogue control strategy covering system-driven preference requests from the corpus is arrived at.</p><p>In the development part, an application-driven development process is adopted where reusable generic components evolve through the iterative and incremental refinement of dialogue systems. The Phase Graph Processor (PGP) design pattern is one such evolved component suggesting a phase-based control of dialogue systems. PGP is a generic and flexible micro architecture accommodating frequent change of requirements inherent of agile, evolutionary system development. As PGP has been used in a series of previous information-providing dialogue system projects, a standard phase graph has been established that covers the second initiative type; user-driven information requests. The phase graph is incrementally refined in order to provide user preference modeling, thus addressing the third initiative type, and multimodality as indicated by the user studies. In the iterative development of the multimodal recommender dialogue system MADFILM the phase graph is coupled with the dialogue control strategy in order to cater for the seamless integration of the three initiative types.</p> / Report code: LiU-TEK-LIC-2004:08.

<p>Huvudfrågan i arbetet är hur produktivitet och säkerhet kombineras i de säkerhetslösningar som existerar idag samt huruvida högre säkerhet leder till ökad komplexitet. Det tillvägagångssätt som använts består av en lastmätning av Secure Socket Layer (SSL), och Pretty Good Privacy (PGP), samt en teoretisk jämförelse av säkerhetslösningarna PGP, SSL och Secure Electronic Transaction (SET). Detta innebär att PGP som har jämförelsevis hög säkerhet när det kommer till kryptering även är den säkerhetslösning är mest resurskrävande. SSL däremot är mindre säkert med betydligt lägre resursanvändning. Generellt kan man med facit i hand säga att den lösning som är säkrast även är den mest komplexa. Den säkerhetsaspekt lastmätningen bygger på är främst krypteringsalgoritm och nyckellängd. Även om dessa aspekter är allmänt vedertagna i säkerhetstänkande måste även hänsyn tas till säkerhetstänkandet som helhet. SET visar ett sätt att kringgå den klassiska motsättningen mellan säkerhet och produktivitet genom att begränsa användningsområdet, men detta på bekostnad av flexibilitet och ger vidare potential för interoperabilitet.</p>

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