ABSTRACT MULTIDRUG RESISTANCE IN LOCALLY ADVANCED BREAST CANCER ATALAY, Mustafa Can Ph. D., Department of Biotechnology Supervisor: Prof. Dr. Ufuk G&Uuml / ND&Uuml / Z June 2004, 70 pages Breast cancer is the most frequently detected cancer among women. Early diagnosis leads to long term survival when the patients are treated with surgery, radiotherapy, chemotherapy, and hormone therapy. Unfortunately, advanced disease could still be encountered in some patients resulting in a poorer prognosis. The primary treatment modality is chemotherapy for this group of patients. Drug resistance is a serious problem resulting in the use of different drugs during chemotherapy and knowing the possibility of resistance before initiating first line chemotherapy may save time and money, and most importantly, may increase patient&rsquo / s survival. Therefore in this study, multidrug resistance is studied in locally advanced breast cancer patients. The breast tissues obtained from 25 patients both before and after chemotherapy were examined for drug resistance. Reverse transcriptase polymerase chain reaction was used for the detection of mdr1 and mrp1 gene expression. In addition, immunohistochemistry technique was used for P-glycoprotein and MRP1 detection. JSB-1 and QCRL-1 monoclonal antibodies were utilized to detect P-glycoprotein and MRP1, respectively. Five patients were unresponsive to chemotherapy. In four of these patients mdr1 gene expression was induced by chemotherapy where as the fifth patient initially had mdr1 gene expression. In addition, Pgp positivity was detected in 9 patients after chemotherapy. Both the induction of mdr1 gene expression (p&lt / 0.001) and Pgp positivity (p&lt / 0.001) during chemotherapy were significantly related with clinical response. On the other hand, mrp1 gene expression and MRP1 positivity were detected in 68% of the patients before the therapy. After chemotherapy, mrp1 expression increased to 84%. Although 80% of the clinically unresponsive patients had mrp1 gene expression, the relation between mrp1 expression and clinical drug response was not strong. Thus, it can be concluded that in locally advanced breast cancer mdr1 gene expression during chemotherapy contributed to clinical unresponsiveness. However, mrp1 gene expression did not correlate strongly with the clinical response. When RT-PCR and immunohistochemistry methods are compared in terms of detection of drug resistance, it seems that both methods gave similar and reliable results.

No description available.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-12-17T17:32:13Z No. of bitstreams: 2 ricardo_costa_sousa.pdf: 978334 bytes, checksum: 95ce2c281d2839fc0eb4f11a7f66ac37 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-12-17T17:32:39Z (GMT) No. of bitstreams: 2 ricardo_costa_sousa.pdf: 978334 bytes, checksum: 95ce2c281d2839fc0eb4f11a7f66ac37 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2015-12-17T17:32:39Z (GMT). No. of bitstreams: 2 ricardo_costa_sousa.pdf: 978334 bytes, checksum: 95ce2c281d2839fc0eb4f11a7f66ac37 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2011 / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / Foi desenvolvida uma Prova de Ganho em Peso (PGP) a pasto com animais da ra?a Guzer? na fazenda Meleiro no munic?pio Curvelo ? MG, oficializada pela Associa??o Brasileira dos Criadores de Zebu (ABCZ). Foram avaliados 45 machos da ra?a Guzer? rec?m-desmamados, com peso e idade iniciais m?dios e desvio padr?o de 219,9 ? 38,05 kg e 325,8 ? 28,0 dias, respectivamente. Na prova os animais consumiram pastagens de Brachiaria brizantha e suplementa??o m?ltipla, receberam tratamento sanit?rio e foram mantidos em condi??es uniformes de manejo por um per?odo de 294 dias. As avalia??es aconteceram a cada 56 dias. A partir desta PGP buscou-se avaliar uma fun??o n?o-linear que melhor descrevesse a curva de crescimento dos animais, estimar o desenvolvimento relativo da ?rea de olho de lombo, medida por ultrassonografia, da circunfer?ncia escrotal e de medidas morfom?tricas em rela??o ao peso vivo de bovinos da ra?a Guzer? atrav?s do estudo do crescimento alom?trico; avaliou-se tamb?m um conjunto de caracter?sticas, por meio de an?lise de componentes principais, visando identificar as caracter?sticas que representam a maior parte da varia??o fenot?pica. As caracter?sticas avaliadas por componentes principais foram: peso aos 205 dias (P205), peso aos 365 dias (P365), peso aos 550 dias (P55), ganho m?dio di?rio (GMD), circunfer?ncia escrotal (CE), altura na garupa (HG), comprimento corporal (CC), circunfer?ncia tor?cica (CT), ?rea de olho de lombo (AOL), estrutura (E), precocidade (P) e musculosidade (M). No presente estudo, a fun??o de Von Bertalanffy foi a que melhor se ajustou para descrever o crescimento dos machos da ra?a Guzer? na PGP a pasto, j? o crescimento alom?trico apresentou-se heterog?nico negativo para todas as caracter?sticas avaliadas em rela??o ao peso vivo. Quanto a an?lise de componentes principais os tr?s primeiros componentes principais explicaram 74,51% da varia??o total dos dados, sendo estes associados aos maiores autovalores e retendo assim, maior vari?ncia dos dados. As caracter?sticas que tiveram maior participa??o nestes componentes foram P205, P e M. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT It was developed in a performance test (PGP) to grazing animals on the farm Guzera Meleiro Curvelo the municipality - MG, officiated by the Brazilian Association of Zebu Breeders (ABCZ). We evaluated 45 male weanling Guzer?, with initial average weight and age and standard deviation of 219.9 ? 38.05 kg and 325.8 ? 28.0 days, respectively. At trial, the animals were grazing Brachiaria brizantha and supplementation multiple received sanitary treatment and were maintained under uniform management for a period of 294 days. The evaluations took place every 56 days. From this PGP sought to evaluate a nonlinear function that best describe the growth curve of animals, estimate the relative development of the loin eye area measured by ultrasound, scrotal circumference and morphometric measurements in relation to body weight bovine Guzera through the study of allometric growth, was also evaluated a set of features through principal component analysis, to identify the characteristics that represent most of the phenotypic variation. The characteristics evaluated by principal components were: weight at 205 days (P205), weight at 365 days (P365), weight at 550 days (P550), average weight gain (ADG), scrotal circumference (SC), hip height (HG), body length (CC), thoracic circumference (TC), loin eye area (LEA), structure (E), precocity (P) and muscle (M). In this study, the Von Bertalanffy function was the best fit to describe the growth of males Guzera grazing on PGP, as the allometric growth presented heterogonic negative for all traits in relation to body weight. In this study, the Von Bertalanffy function was the best fit to describe the growth of males Guzera grazing on PGP, as the allometric growth presented heterogonic negative for all traits in relation to body weight. As for principal components analysis the first three principal components explained 74.51% of the total variation of data, which are associated with larger eigenvalues and thus retaining, greater data variance. The characters with the highest participation in these components were P205, P and M.

Submitted by PAULO GABRIEL FRANCO DOS SANTOS null (paulogabriel22@yahoo.com.br) on 2017-07-28T17:35:29Z No. of bitstreams: 1 Tese - Paulo Gabriel F dos Santos - 2017.pdf: 1759717 bytes, checksum: b00e6d4d3a56c3e3b21a857eac1c0924 (MD5) / Approved for entry into archive by Luiz Galeffi (luizgaleffi@gmail.com) on 2017-08-03T14:39:41Z (GMT) No. of bitstreams: 1 santos_pgf_dr_bauru.pdf: 1759717 bytes, checksum: b00e6d4d3a56c3e3b21a857eac1c0924 (MD5) / Made available in DSpace on 2017-08-03T14:39:41Z (GMT). No. of bitstreams: 1 santos_pgf_dr_bauru.pdf: 1759717 bytes, checksum: b00e6d4d3a56c3e3b21a857eac1c0924 (MD5) Previous issue date: 2017-05-29 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A presente tese busca sustentar um modelo de formação de professores baseado na perspectiva da experiência formativa de Walter Benjamin. Diante do declínio da experiência na modernidade, o desafio desta investigação é direcionado no sentido de criar um espaço favorável à experiência formativa, reivindicando relações orgânicas entre sujeito e objeto de conhecimento, do trabalhador com os produtos e o contexto do seu trabalho e como sobrevivência à reiteração do sujeito em tempos de industrialização e de império da racionalidade instrumental. Busca-se também desenvolver um caminho formativo para a profanação da Ciência e a Tecnologia pela via do tratamento das Questões Sociocientíficas, isto é, a reapropriação destes empreendimentos pelo ser humano como produção cultural, histórica, dinâmica, de modo a desmistificá-las e questionar sua suposta neutralidade e linearidade ascendente. Assim, o objetivo central da pesquisa é delinear a natureza de um contexto formativo de professores com intencionalidades de se constituir como comunidade de experiência e caracterizar o caráter do tratamento de Questões Sociocientíficas nesse contexto. O processo metodológico de pesquisa, de natureza qualitativa, inclui a participação ativa do pesquisador em reuniões periódicas com os professores, esforços para criar um lugar de construção de sentido, produção coletiva de conhecimentos e de conteúdos para trabalhar em sala de aula pelos professores envolvidos. Entendendo que a narração é uma expressão genuína da experiência e que narrar se converte também em uma experiência, as narrações dos professores do Pequeno Grupo de Pesquisa (PGP) são as fontes de informação para o desenvolvimento da análise, bem como as experiências do pesquisador. A análise é desenvolvida sob a perspectiva de constelação que consiste em um momento analítico-conceitual, com categorias criadas com base nos referenciais teóricos adotados, e representacional, que consiste na justaposição das categorias, constituição de imagens dialéticas e reagrupamento das imagens, configurando um movimento do particular concreto para a totalidade social apreendida pela experiência de pesquisa. De forma geral, caminhar entre dois mundos conceituais e concretos é transitar em um caminho tensionado, a figura ambígua do pesquisador como estrangeiro e como figura central garante a sustentação da interface Universidade-Escola, o trabalho docente povoado de fantasmagorias e incluso no fluxo da multidão requer interrupção e objetivação da realidade para torná-la elemento experiencial e o contexto de formação se constitui como uma promessa de redenção para os docentes, o exercício de rememoração e construção de significados contribui para a conformação de valores e práticas no contexto formativo e a elaboração e tratamento de Questões Sociocientíficas em um contexto de experiência formativa reclama elementos de legitimidade, relevância dos conteúdos e das práticas, abertura para a criatividade e autoria, profanação dos empreendimentos da ciência e da tecnologia e constante retorno para o lugar de formação para sua sustentação e produção de sentido. / This thesis seeks to sustain a teacher education model based on the perspective of the formative experience of Walter Benjamin. Faced with the decline of experience in modernity, the challenge of this research is directed towards creating a favorable space to formative experience, demanding for organic relations between subject and object of knowledge, worker and the products and the context of its work, as survival to the reification of the subject in times of industrialization and empire of instrumental rationality. The aim is to also develop a formative way to the profanation of Science and Technology via the treatment of socio-scientific issues, i.e. the reappropriation of these institutions by humans as cultural, historical and dynamic production, in order to demystify them and to inquire their supposed neutrality and ascending linearity. Thus, the central objective of the research is to delineate the nature of a formative context of teachers with intentions to constitute itself as a community of experience and to characterize the character of the treatment of Socio-Scientific Issues in this context. The methodological research process, of qualitative nature, includes the active participation of the researcher in regular meetings with teachers, efforts to create a place of construction of meaning, collective production of knowledge and content to work in the classroom by the teachers involved. Understanding that the narration is a genuine expression of experience and narrate also becomes experience, the narrations of the teachers in the Small Group of Reasearch (SGR) are the sources of information for the development of analysis, as well as the experiences of the researcher. The analysis is developed in the constellation perspective which consists of an analytical-conceptual moment, with categories created based on the theoretical framework adopted, and representational moment, which is the juxtaposition of categories, constitution of dialectical images and images reassembly, setting a movement from the particular concrete to the social totality apprehended by the research’s experience. In general, to walk between two conceptual and concrete worlds is to move on a tense path, the ambiguous figure of the researcher as a foreigner and the central figure guarantees the support of the University-School interface, the teaching work populated by phantasmagorias and as part of the crowd flow requires interruption and objectification of reality to make it an experiential element, the context of formation represents a promise of redemption for teachers, the exercise of rememoring and constructing meanings contributes to the conformation of values and practices in the formative context, and the elaboration and treatment of Socio-Scientific Issues in a context of formative experience claims for elements of legitimacy, relevance of contents and practices, openness to creativity and authorship, profanation of science and technology enterprises and constant return to the formation place for their support and production of sense.

Central mechanisms involving aldosterone - mineralocorticoid receptor (MR) activation mediate the increase in sympathetic tone after myocardial infarction (MI). We hypothesized that an increase in cardiac sympathetic activity (CSA) post MI facilitates cardiac sympathetic axonal sprouting, and that central MR blockade attenuates CSA and reduces cardiac sympathetic hyperinnervation post MI. Western blotting and qRT-PCR were used to assess protein and gene expression, and fluorescent immunohistochemistry was used to study changes in sympathetic innervation. Tyrosine hydroxylase (TH) and Norepinephrine transporter protein content in the non-infarcted base of the heart remained unaltered. In contrast, protein gene product (PGP 9.5) protein was significantly increased 2 fold in the base of the heart, and 6 fold in the peri-infarct area at 1 wk post MI, and associated with increased ubiquitin expression. Cardiac myocytes rather than sympathetic axons were identified as the main source of elevated PGP 9.5 expression. At the infarct border sympathetic hyperinnervation was observed with a 4 fold increase in growth associated protein 43 (GAP 43), a 2 fold increase in TH and a 50% increase in PGP 9.5 positive fibers when compared to the epicardial side of the left ventricle in sham rats. Central infusion of the MR blocker eplerenone at 5 ug/day for 9 days post MI markedly attenuated the increase in TH, GAP 43 and PGP 9.5 nerve densities at the infarct border. Central MR blockade may attenuate sympathetic hyperinnervation by several mechanisms, including decreasing CSA post MI, or affecting expression or function of nerve growth factor protein. Marked PGP 9.5 expression occurs in cardiomyocytes early post MI, which may contribute to the increase in ubiquitin and the early cardiac remodeling post MI.

Tato diplomová práce se zabývá problémem bezpečné distribuce software. Je navrženo zlepšení s pomocí doménového systému, který je použit pro uložení verifikačních klíčů, potřebných pro ověření integrity balíků stáhnutých pomocí správce balíků. Navíc je navržena rozšířená verze, které se zabývá zabezpečením metadat repositářů. Obě verze jsou implementovány v jazyce Python a integrovány do správce balíků dnf. Tato implementace je otestována ve virtuálním prostředí, diskutována a zhodnocena z hlediska způsobené zátěže.

Das Dokument beschreibt in kurzer Form das Sicherheitskonzept im Archivsystem MONARCH.Die archivierten Dokumente werden durch verschiedene Hash-Algorithmen und digitale Signaturen vor nachträglichen Veränderungen geschützt. Dadurch kann dem Benutzer von MONARCH die Unversehrtheit der archivierten Publikationen garantiert werden.

Vortrag ueber das Warum und Wie der Kryptografie mit oeffentlichen Schluesseln am Beispiel von GNU Privacy Guard

The work in this thesis addresses design and development of multimodal dialogue recommender systems for the home context-of-use. In the design part, two investigations on multimodal recommendation dialogue interaction in the home context are reported on. The first study gives implications for the design of dialogue system interaction including personalization and a three-entity multimodal interaction model accommodating dialogue feedback in order to make the interaction more efficient and successful. In the second study a dialogue corpus of movie recommendation dialogues is collected and analyzed, providing a characterization of such dialogues. We identify three initiative types that need to be addressed in a recommender dialogue system implementation: system-driven preference requests, userdriven information requests, and preference volunteering. Through the process of dialogue distilling, a dialogue control strategy covering system-driven preference requests from the corpus is arrived at. In the development part, an application-driven development process is adopted where reusable generic components evolve through the iterative and incremental refinement of dialogue systems. The Phase Graph Processor (PGP) design pattern is one such evolved component suggesting a phase-based control of dialogue systems. PGP is a generic and flexible micro architecture accommodating frequent change of requirements inherent of agile, evolutionary system development. As PGP has been used in a series of previous information-providing dialogue system projects, a standard phase graph has been established that covers the second initiative type; user-driven information requests. The phase graph is incrementally refined in order to provide user preference modeling, thus addressing the third initiative type, and multimodality as indicated by the user studies. In the iterative development of the multimodal recommender dialogue system MADFILM the phase graph is coupled with the dialogue control strategy in order to cater for the seamless integration of the three initiative types.

Bakgrund: Lumbal smärta (LP) och bäckengördelssmärta (PGP) är vanligt förekommande för gravida kvinnor. Detta beror på olika faktorer, till exempel mekaniska, hormonella och övriga livsstilsrelaterade faktorer. Denna typ av problematik är vanligt förekommande under den tredje trimestern av graviditeten. Denna period ställer krav på den gravida kvinnan då fostret är som störst under denna period. Fysisk aktivitet har enligt tidigare forskning haft en positiv inverkan på lumbal smärta och bäckengördelsssmärta under graviditet. Den träning som rekommenderas under graviditet är muskelstärkande träning, framför allt träning för bäckenbottenmuskulaturen och bålen. Syfte: Syftet med denna litteraturöversikt var att sammanställa litteratur gällande hur fysisk träning kan påverka ländryggssmärta och bäckengördelssmärta hos gravida kvinnor i den tredje trimestern. Metod: En litteraturstudie utfördes. PICO modellen användes för att avgränsa frågeställningen. Därefter tillämpades en uttömmande sökstrategi för att hitta relevanta artiklar. Databaserna PubMed, CINAHL och AMED användes. Inkluderade artiklar kvalitetsgranskades med hjälp av SBU:s granskningsmallar. Resultat: Åtta artiklar inkluderades. Dessa visade att fysisk träning har reducerande effekt på Lumbal smärta och bäckengördelssmärta. Träning för bäckenbottenmuskulatur, bål och nedre extremitet studerades specifikt och hade en positiv inverkan på aktuell problematik. Diskussion: Eftersom flera olika typer av artiklar inkluderades, blev evidensgraden begränsad. Det fanns inte tillräckligt med relevanta RCT-studier att tillgå i valda databaser för att uppnå hög evidensgrad. Konklusion: Olika typer av fysisk träning har effekt på aktuell problematik. Vidare forskning behövs för att kunna dra slutsatser gällande ämnet.