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41	Sticky triangles: New tools for experimental phasing of biological macromolecules / Sticky triangles: Neue Werkzeuge für die experimentelle Phasierung von biologischen Makromolekülen Beck, Tobias 16 September 2010 (has links) No description available. 540 Chemie Chemistry Strukturbestimmung Strukturlösung biologische Makromoleküle Proteine experimentelle Phasierung anomale Dispersion Schweratomderivatisierung structure determination structure solution biological macromolecules proteins experimenal phasing anomalous dispersion heavy-atom derivatisation 35.62 35.76 35.90 42.13 SP 000: Strukturchemie SXL 440: Struktur {Biochemie} WF 100: Methoden {Molekularbiologie Gentechnologie}
42	Aplikace metodiky Projektového managementu na projektu v podniku / Project Management Methodology Application on Company´s Project Salaj, Tomáš January 2011 (has links) Diploma thesis focuses on the development of parking building with commercial premises Rychtářka in Plzeň, which is realized in the form of public and private sector partnership. The thesis deals with effective application of project management methods in light of the general contractor Hermosa Parking Plzeň a.s. Information and knowledge gained in the theoretical part of the thesis are then used in the practical part for the design of project management methodics on development project Rychtářka. The outcome of practical part of the thesis is project management methodic, which should help to secure the optimal solution for complex structured process that leads to the realization of parking building Rychtářka and the compliance with all the specific characteristics of development projects.
43	Stochastic Modeling of Electricity Prices and the Impact on Balancing Power Investments / Stokastisk modellering av elpriser och effekten på investeringar i balanskraft Ruthberg, Richard, Wogenius, Sebastian January 2016 (has links) Introducing more intermittent renewable energy sources in the energy system makes the role of balancing power more important. Furthermore, an increased infeed from intermittent renewable energy sources also has the effect of creating lower and more volatile electricity prices. Hence, investing in balancing power is prone to high risks with respect to expected profits, which is why a good representation of electricity prices is vital in order to motivate future investments. We propose a stochastic multi-factor model to be used for simulating the long-run dynamics of electricity prices as input to investment valuation of power generation assets. In particular, the proposed model is used to assess the impact of electricity price dynamics on investment decisions with respect to balancing power generation, where a combined heat and power plant is studied in detail. Since the main goal of the framework is to create a long-term representation of electricity prices so that the distributional characteristics of electricity prices are maintained, commonly cited as seasonality, mean reversion and spikes, the model is evaluated in terms of yearly duration which describes the distribution of electricity prices over time. The core aspects of the framework are derived from the mean-reverting Pilipovic model of commodity prices, but where we extend the assumptions in a multi-factor framework by adding a functional link to the supply- and demand for power as well as outdoor temperature. On average, using the proposed model as a way to represent future prices yields a maximum 9 percent overand underprediction of duration respectively, a result far better than those obtained by simpler models such as a seasonal profile or mean estimates which do not incorporate the full characteristics of electricity prices. Using the different aspects of the model, we show that variations of electricity prices have a large impact on the investment decision with respect to balancing power. The realized value of the flexibility to produce electricity in a combined heat and power plant is calculated, which yields a valuation close to historical realized values. Compared with simpler models, this is a significant improvement. Finally, we show that by including characteristics such as non-constant volatility and spiky behavior in investment decisions, the expected value of balancing power generators, such as combined heat and power plants, increases. / I takt med att fler intermittenta förnyelsebara energikällor tillför el i dagens energisystem, blir också balanskraftens roll i dessa system allt viktigare. Vidare så har en ökning av andelen intermittenta förnyelsebara energikällor även effekten att de bidrar till lägre men också mer volatila elpriser. Därmed är även investeringar i balanskraft kopplade till stora risker med avseende på förväntade vinster, vilket gör att en god representation av elpriser är central vid investeringsbeslut. Vi föreslår en stokastisk flerfaktormodell för att simulera den långsiktiga dynamiken i elpriser som bas för värdering av generatortillgångar. Mer specifikt används modellen till att utvärdera effekten av elprisers dynamik på investeringsbeslut med avseende på balanskraft, där ett kraftvärmeverk studeras i detalj. Eftersom huvudmålet med ramverket är att skapa en långsiktig representation av elpriser så att deras fördelningsmässiga karakteristika bevaras, vilket i litteraturen citeras som regression mot medelvärde, säsongsvariationer, hög volatilitet och spikar, så utvärderas modellen i termer av årlig prisvaraktighet som beskriver fördelningen av elpriser över tid. Kärnan i ramverket utgår från Pilipovic-modellen av råvarupriser, men där vi utvecklar antaganden i ett flerfaktorramverk genom att lägga till en länkfunktion till tillgång- och efterfrågan på el samt utomhustemperatur. Vid användande av modellen som ett sätt att representera framtida priser, fås en maximal över- och underprediktion av prisvaraktighet om 9 procent, ett resultat som är bättre än det som ges av enklare modellering såsom säsongsprofiler eller enkla medelvärdesestimat som inte tar hänsyn till elprisernas fulla karakteristika. Till sist visar vi med modellens olika komponenter att variationer i elpriser, och därmed antaganden som används i långsiktig modellering, har stor betydelse med avseende på investeringsbeslut i balanskraft. Det realiserade värdet av flexibiliteten att producera el för ett kraftvärmeverk beräknas, vilket ger en värdering nära faktiska realiserade värden baserade på historiska priser och som enklare modeller inte kan konkurrera med. Slutligen visar detta också att inkluderandet av icke-konstant volatilitet och spikkarakteristika i investeringsbeslut ger ett högre förväntat värde av tillgångar som kan producera balanskraft, såsom kraftvärmeverk. Energy investment investment valuation renewable energy production electricity price modeling long-term combined heat and power CHP balancing power intermittent renewable energy modeling Pilipovic model multi-factor model sinusoidal regression Ornstein-Uhlenbeck estimation electricity price duration prediction Nord Pool Sweden electricity market future energy systems phasing out nuclear power energy policy. Mathematical Analysis Matematisk analys
44	Structural Investigation of Processing α-Glucosidase I from Saccharomyces cerevisiae Barker, Megan 20 August 2012 (has links) N-glycosylation is the most common eukaryotic post-translational modification, impacting on protein stability, folding, and protein-protein interactions. More broadly, N-glycans play biological roles in reaction kinetics modulation, intracellular protein trafficking, and cell-cell communications. The machinery responsible for the initial stages of N-glycan assembly and processing is found on the membrane of the endoplasmic reticulum. Following N-glycan transfer to a nascent glycoprotein, the enzyme Processing α-Glucosidase I (GluI) catalyzes the selective removal of the terminal glucose residue. GluI is a highly substrate-specific enzyme, requiring a minimum glucotriose for catalysis; this glycan is uniquely found in biology in this pathway. The structural basis of the high substrate selectivity and the details of the mechanism of hydrolysis of this reaction have not been characterized. Understanding the structural foundation of this unique relationship forms the major aim of this work. To approach this goal, the S. cerevisiae homolog soluble protein, Cwht1p, was investigated. Cwht1p was expressed and purified in the methyltrophic yeast P. pastoris, improving protein yield to be sufficient for crystallization screens. From Cwht1p crystals, the structure was solved using mercury SAD phasing at a resolution of 2 Å, and two catalytic residues were proposed based upon structural similarity with characterized enzymes. Subsequently, computational methods using a glucotriose ligand were applied to predict the mode of substrate binding. From these results, a proposed model of substrate binding has been formulated, which may be conserved in eukaryotic GluI homologs. Biomolecular structure Eukaryotic glycosylation glycosylation N-glycan N-linked glycosylation Carbohydrate biochemistry Inhibitor antiviral therapeutic Structure-based drug design glucosidase glycosidase glycoside hydrolase post-translational modification enzyme enzyme mechanism inverting mechanism structural biology protein structure 6xHis tag Crystal packing sugar substrate X-ray crystallography single anomalous dispersion PHENIX heavy-atom phasing docking autodock autodock vina biophysical methods tryptophan fluorescence glucose oxidase activity assay enzyme inhibition Protein expression Protein purification Pichia pastoris Saccharomyces cerevisiae fondue AOX1 promoter glycoside hydrolysis substrate binding model molecular dynamics molecular dynamics Binding site Active site cleft Endoplasmic reticulum Glycan processing Glycan trimming protein-protein interactions cell-cell communication X-ray diffraction crystallization secreted protein substrate selectivity kojibiose glucotriose miglitol deoxynojirimycin Glc3Man9GlcNAc2 free oligosaccharide species GluI Cwh41 Cwh41p Cwht1p 0306 0307 0760
45	Structural Investigation of Processing α-Glucosidase I from Saccharomyces cerevisiae Barker, Megan 20 August 2012 (has links) N-glycosylation is the most common eukaryotic post-translational modification, impacting on protein stability, folding, and protein-protein interactions. More broadly, N-glycans play biological roles in reaction kinetics modulation, intracellular protein trafficking, and cell-cell communications. The machinery responsible for the initial stages of N-glycan assembly and processing is found on the membrane of the endoplasmic reticulum. Following N-glycan transfer to a nascent glycoprotein, the enzyme Processing α-Glucosidase I (GluI) catalyzes the selective removal of the terminal glucose residue. GluI is a highly substrate-specific enzyme, requiring a minimum glucotriose for catalysis; this glycan is uniquely found in biology in this pathway. The structural basis of the high substrate selectivity and the details of the mechanism of hydrolysis of this reaction have not been characterized. Understanding the structural foundation of this unique relationship forms the major aim of this work. To approach this goal, the S. cerevisiae homolog soluble protein, Cwht1p, was investigated. Cwht1p was expressed and purified in the methyltrophic yeast P. pastoris, improving protein yield to be sufficient for crystallization screens. From Cwht1p crystals, the structure was solved using mercury SAD phasing at a resolution of 2 Å, and two catalytic residues were proposed based upon structural similarity with characterized enzymes. Subsequently, computational methods using a glucotriose ligand were applied to predict the mode of substrate binding. From these results, a proposed model of substrate binding has been formulated, which may be conserved in eukaryotic GluI homologs. Biomolecular structure Eukaryotic glycosylation glycosylation N-glycan N-linked glycosylation Carbohydrate biochemistry Inhibitor antiviral therapeutic Structure-based drug design glucosidase glycosidase glycoside hydrolase post-translational modification enzyme enzyme mechanism inverting mechanism structural biology protein structure 6xHis tag Crystal packing sugar substrate X-ray crystallography single anomalous dispersion PHENIX heavy-atom phasing docking autodock autodock vina biophysical methods tryptophan fluorescence glucose oxidase activity assay enzyme inhibition Protein expression Protein purification Pichia pastoris Saccharomyces cerevisiae fondue AOX1 promoter glycoside hydrolysis substrate binding model molecular dynamics molecular dynamics Binding site Active site cleft Endoplasmic reticulum Glycan processing Glycan trimming protein-protein interactions cell-cell communication X-ray diffraction crystallization secreted protein substrate selectivity kojibiose glucotriose miglitol deoxynojirimycin Glc3Man9GlcNAc2 free oligosaccharide species GluI Cwh41 Cwh41p Cwht1p 0306 0307 0760

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