A bacterium and a fungus identified as a Pseudomonas sp. and a Penicillium sp. were isolated by the soil perfusion enrichment technique, and are able to utilize phloroglucinol as the sole carbon source in a medium containing various inorganic salts. Chemical and physical factors affecting growth of the organism and the rate of phloroglucinol utilization were studied. A new pathway for the decomposition of phloroglucinol by the Pseudomonas sp. has been proposed. This pathway involves the removal of a hydroxyl group from phloroglucinol forming a conjugated phenolic compound followed by the oxidation of the bond between the third and fourth carbon atoms forming beta-ketoadipic acid. The enzymes involved in this pathway are not constitutive, but are induced through the formation of adaptive enzymes. The enzymatic reactions were NADPH2 dependent. The Km of the enzyme for phloroglucinol was determined. The Penicillium sp. utilizes phloroglucinol and accumulates a product which is not further metabolized.

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The purpose of this investigation was to form various derivatives of phlorogluc inol and to treat these with furfural in hydrochloric acid solution to see if, in this manner, a clearer understanding of the nature of the reaction between furfural and phloroglucinol night not ue ascertained.

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The following information, as revealed in these studies, is claimed to be a contribution to knowledge. A bacterium and a fungus identified as a Pseudomonas sp. and a Penicillium sp. were isolated by the soil perfusion enrichment technique, and are able to utilize phloroglucinol as the sole carbon source in a medium containing various inorganic salts. [...]

Thesis (M.S.)--University of Wisconsin--Madison, 1969. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

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O floroglucinol é um composto fenólico precursor de diversas moléculas com atividades biológicas já descritas na literatura, com destaque para a antidepressiva. O modelo experimental com zebrafish tem sido bastante utilizado em várias linhas de pesquisa biológica, como, por exemplo, para avaliação da atividade anticonvulsivante. A partir de estudos que mostram uma correlação entre compostos antidepressivos e anticonvulsivantes, nosso grupo administrou floroglucinol e dois derivados sintéticos (composto 7 e composto 8) em zebrafish para observação da atividade locomotora e exploratória no open tank e, posteriormente, à avaliação através do modelo convulsivo induzido por pentilenotetrazol (PTZ). Além disso, os níveis de captação de glutamato e a toxicidade dos compostos foram avaliados em cérebro total de zebrafish. O comportamento dos animais não sofreu alteração em relação ao controle para nenhum dos compostos testados. O composto 7 aumentou significativamente o tempo para os animais atingirem a primeira convulsão além de reduzir a intenidade da crise convulsiva. Também se observou aumento na captação de glutamato para esse composo, sem sinais de toxicidade envolvidos. Desta forma, nossos resultados contribuem para a busca de compostos potencialmente ativos frente a crises convulsivas induzidas por PTZ. / Phloroglucinol, a phenolic compound, which is precursor of several molecules with biological activities are described in the literature, mainly for antidepressant activity. The zebrafish experimental model has been widely used in many kinds of biological research, for example, to evaluate the anticonvulsant activity. From studies that shows correlation between antidepressants and anticonvulsant compounds, our group managed phloroglucinol and two synthetic derivatives (compound 7 and compound 8) in zebrafish in order to observe the locomotor and exploratory activity on open tank and subsequently, conduct the evaluation through the seizure model induced by pentylenetetrazol (PTZ). Furthermore, glutamate uptake and toxicity levels of the compounds were evaluated in zebrafish’s whole brain. The animals' behavior did not change compared to control for any of the tested compounds. The compound 7 increased significantly the time for the animals reach the first seizure and reduce the seizure intensity. It was also observed an increase in glutamate uptake for this compound without signs of toxicity involved. Thus, our results contribute to the search for potentially active compounds against seizures induced by PTZ.