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Photodynamic effects of meta-tetra-(hydroxyphenyl)-chlorin (mTHPC) and pyropheophorbide-a methyl ester (MPPa) in tumour cells : a mechanistic studySun, Xun 01 January 2001 (has links)
No description available.
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Synthesis and Characterization of New Silicon Phthalocyanines and Nonyl Acridine Orange Analogues for Photodynamic Therapy StudiesZhang, Ping January 2009 (has links)
Thesis (Ph.D.)--Case Western Reserve University, 2009 / Abstract Department of Chemistry Title from PDF (viewed on 13 April 2009) Available online via the OhioLINK ETD Center
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Photodynamic therapy (PDT) in malignant glioma: is intra-tumoural injection of the photosensitizer by stereotactic technique superior to conventional intravenous administration?.January 1996 (has links)
Chan Yung. / Publication date from spine. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves 80-95). / Acknowledgement --- p.3 / Abstract --- p.4 / Chapter Chapter 1 --- Introduction --- p.7 / Chapter "1,1" --- Current status in treatment of gliomas --- p.7 / Chapter 1.1.1 --- Surgery of gliomas --- p.7 / Chapter 1.1.2 --- Radiation therapy of gliomas --- p.9 / Chapter 1.1.3 --- Chemotherapy for gliomas --- p.10 / Chapter 1.1.4 --- New modalities --- p.11 / Chapter 1.2 --- Basic principle of photodynamic therapy --- p.13 / Chapter 1.2.1 --- Photosensitizer --- p.13 / Chapter 1.2.2 --- Photosensitising effect of HpD --- p.13 / Chapter 1.2.3 --- Mechanism of selectivity in PDT --- p.15 / Chapter 1.2.4 --- Selective localization and the blood brain barrier --- p.16 / Chapter 1.2.5 --- Cytotoxic effect of PDT --- p.18 / Chapter 1.2.6 --- PDT on normal brain --- p.19 / Chapter 1.3 --- Photodynamic therapy in clinical neurosurgery --- p.21 / Chapter 1.4 --- The aims of the study --- p.26 / Chapter Chapter 2 --- Materials and methods --- p.28 / Chapter 2.1 --- Patient population --- p.28 / Chapter 2.2 --- Photosensitizer --- p.28 / Chapter 2.3 --- Administration of HpD --- p.29 / Chapter 2.4 --- Measurement of HpD --- p.30 / Chapter 2.5 --- Fluorescence microscopic examination --- p.32 / Chapter 2.6 --- Confocal laser scanning microscopic examination --- p.33 / Chapter Chapter 3 --- Results --- p.38 / Chapter 3.1 --- HpD measurement --- p.38 / Chapter 3.2 --- Fluorescence microscope examination --- p.40 / Chapter 3.3 --- Confocal laser scanning microscope (CLSM) examination --- p.41 / Chapter Chapter 4 --- Discussion --- p.56 / Chapter 4.1 --- Quantifying HpD level --- p.58 / Chapter 4.2 --- Quantifying HpD in the intravenous administration group --- p.59 / Chapter 4.3 --- Quantifying HpD in the intratumoural injection group --- p.63 / Chapter 4.4 --- Flourescence microscopic study of HpD distribution of malignant gliomas --- p.67 / Chapter 4.5 --- Confocal laser scanning microscopy (CLSM) study of HpD localization of malignant gliomas --- p.72 / Chapter 4.6 --- Conclusion --- p.74 / Chapter Chapter 5 --- Summary --- p.76 / Chapter Chapter 6 --- References --- p.80
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Toward dual and targeted cancer therapy with novel phthalocyanine-based photosensitizers. / CUHK electronic theses & dissertations collectionJanuary 2012 (has links)
酞菁是一種廣泛應用的多用途功能材料。本研究工作的目的是探索酞菁作為用於靶向光動力療法 (PDT) 的可激活的和高效光敏劑的潛力。就這研究課題,本論文描述了ー系列精心設計的酞菁光敏劑的合成、其光譜表徵、光物理屬性和體外光動力活性。 / 第一章概述了光動力療法,包括其歷史發展、光物理和生物機制及臨床應用現狀。本章介紹各種不同類型的光敏劑,其中重點介紹了酞菁,因它被視為最有前途的第二代光敏劑之一。為了提高療效,過往有不少努力都投放在去把光敏劑功能化,以實現它在化學療法和光動力雙效治療及靶向治療的作用。本章的結尾部分,我們更會引用相關的例子去討論和解釋這種先進治療方式的概念以及近期的發展。 / 含鉑藥物已作為臨床化療藥物廣泛應用於多種癌症的治療。我們設計了一種新型的酞菁鉑配合物共軛體系,以取得光動力和化學療法的雙重效果。第二章主要描述了由鋅(II)酞菁和具有抗癌性的奧沙利鉑衍生物構成的共軛體系的合成方法和光譜特性,同時對該共軛體系基本的光物理性能,聚合行為和體外光動力性能進行了考察,並與一些化合物模型進行了對比。該共軛體系展示出一種協同效應。在HT29 人結腸腺癌細胞內,奧沙利鉑的存在導致該共軛體系在黑暗裡產生暗毒性;在光照下,基於鋅(II)酞菁的存在,它產生了增强的細胞毒素效應。這種高效光動力行為也可以歸結為其較高的細胞攝取和活性氧 (ROS) 產生的功效。該共軛體系更傾向定位於細胞溶酶體並誘使主要通過細胞凋亡的細胞死亡。 / 多胺是普遍存在於細胞的陽離子,並在細胞的繁殖和分化中起着多重作用。許多腫瘤細胞表現出具有較高的多胺含量並且激活多胺載體以導入外源多胺來維持快速的細胞分裂活動。因此,與多胺類化合物的結合是提高光敏材料針對腫瘤細胞的方法之一。第三章描述了一系列多胺取代的鋅(II)酞菁的合成、光譜表徵和光物理特性,並考察了它們對於B16 黑色素瘤和中國倉鼠卵巢 CHO 細胞的光動力行為。同時對其細胞攝取率、亞細胞定位和細胞死亡機制進行了報導。 / 除了與這些腫瘤特異載體共軛連接,可被腫瘤相關刺激激活的光敏劑也是能提高腫瘤選擇性的一個好方法。谷胱甘肽 (GSH) 在細胞質中是最豐富的硫醇,也是在生物化學過程中主要的還原劑。細胞內的谷胱甘肽 (ca. 10 mM) 與細胞外部 (ca. 2 μM) 相比具有較高的濃度。 因此,我們可以利用以這個特性來啟動光敏劑。第四章報告了一種具有還原效應的矽(IV)酞菁。該化合物軸向由兩個二茂鐵基查爾酮衍生物通過二硫鍵取代,並易於在還原條件下快速斷裂。我們通過不同的光譜測試,對該化合物基本的光物理性能及其在還原試劑作用下的斷裂動力學進行了研究。我們也同時考察了存在和不存在外部還原試劑條件下,其對於MCF-7 人乳腺癌細胞的體外光動力行為,包括細胞毒素效應、細胞攝取和亞細胞定位。 / 為了進一步提高腫瘤的選擇性,發展一種可同時被兩種腫瘤相關刺激激活的光敏劑也是體現靶向治療的方法之一。 癌細胞除了比正常細胞含有較高的谷胱甘肽濃度外,它的另一種特性是其外區域的pH 值 (ca. 6.8) 比周圍正常組織(ca. 7.3) 相對較低。這種獨特的特徵也是一種機制來啟動光敏劑。第五章主要探討一種具有pH 和還原雙回應的矽(IV)酞菁。該化合物軸向分別由二茂鐵衍生物通過二硫鍵和腙鍵取代。由於連接鍵在生理的pH 值和低濃度還原試劑或其中一項的條件下不會斷裂,所以在二茂鐵的淬滅的影響下,光敏劑維持在光動力惰性的狀態。但是,在酸性和高濃度還原試劑的情況下,光敏劑會被激活。我們會在這一章探討這些外來刺激對光敏劑的光物理特性和體外光動力行為的影響。 / 第六章會就本項研究作出總結,而第七章則報導實驗程序。第八章列舉了本論文所引用的參考文獻。 / 論文的最後會附上所有新化合物的氫及碳核磁共振譜圖。 / Phthalocyanines are versatile functional materials for a wide range of applications. This research work aims to explore their potential as activatable and efficient photosensitizers for targeted photodynamic therapy (PDT). This thesis describes the synthesis, spectroscopic characterization, photophysical properties, and in vitro photodynamic activities of several series of carefully designed phthalocyanine-based photosensitizers. / Chapter 1 presents an overview of PDT, including its historical development, photophysical and biological mechanisms, and current clinical applications. Various classes of photosensitizers are introduced with emphasis put on phthalocyanines, which have emerged as a promising class of second-generation photosensitizers for PDT. In order to enhance the therapeutic efficacy, considerable effort has been expended to functionalize the photosensitizers with a view to achieving dual and targeted therapy. The concept and recent development of this advanced modality is discussed and illustrated with relevant examples at the end of this chapter. / Platinum-based drugs have been widely used as clinical chemotherapeutic drugs for the treatment of a variety of cancers. In order to bring about a dual photodynamic and chemotherapeutic effect, we have designed a novel phthalocyanine-platinum complex conjugate. Chapter 2 presents the synthesis and spectroscopic characterization of this conjugate, which comprises of a zinc(II) phthalocyanine and an oxaliplatin derivative that is known to have antitumor activity. The basic photophysical properties, aggregation behavior, and in vitro photodynamic activities of this conjugate have also been investigated and compared with those of some model compounds. This conjugate demonstrates a synergistic effect in which it shows a cytotoxic effect in the dark due to the oxaliplatin moiety and an enhanced cytotoxicity upon illumination due to the phthalocyanine unit toward the HT29 human colon adenocarcinoma cells. The high photodynamic activity can also be attributed to its higher cellular uptake and reactive oxygen pecies (ROS) generation efficiency. This conjugate shows preferential localization in the lysosomes and induces cell death mainly through apoptosis. / Polyamines are ubiquitous cellular cations that play multifunctional roles in cell proliferation and differentiation. Many tumors cells have been shown to contain elevated polyamine levels and active polyamine transporters for importing exogenous polyamines in order to sustain rapid cell division. As a result, conjugation of polyamine analogues is one of the potential strategies to improve the tumor-targeting property of photosensitizers. Chapter 3 describes the synthesis, spectroscopic characterization, and photophysical properties of a series of polyamine-substituted zinc(II) phthalocyanines. Their photodynamic activities toward B16 melanoma and Chinese hamster ovary (CHO) cells have been investigated. Their cellular uptake, subcellular localization, and cell death mechanism are also reported herein. / Apart from conjugation to these tumor-specific vectors, the use of photosensitizers that can be activated by tumor-associated stimuli is also a promising approach to enhance the tumor selectivity. Glutathione (GSH) is the most abundant thiol in cytoplasm and the major reducing agent in many biochemical processes. The much higher intracellular GSH concentration (ca. 10 mM) compared with the extracellular levels (ca. 2 μM) provides a mechanism for activating photosensitizers. Chapter 4 reports the molecular design and development of a novel redox-responsive silicon(IV) phthalocyanine axially substituted with two ferrocenyl-chalcone derivatives via disulfide bonds, which are prone to rapid cleavage under a reducing environment. The basic photophysical properties of this compound and its cleavage kinetics upon exposure to a reductive stimulant have been studied by various spectroscopic methods. Its in vitro photodynamic activities including cytotoxicity, cellular uptake, and subcellular localization towa d MCF-7 human breast cancer cells, both in the absence and presence of an external reducing agent, have also been examined. / To further enhance the tumor specificity, development of activatable photosensitizers that can be activated concurrently by two different tumor-associated stimuli is also a promising strategy toward targeted PDT. Aside from the difference in GSH content between tumor and normal tissues, another unique feature of tumors is that their extracellular pH (ca. 6.8) is relatively lower as compared with that around the normal tissues (ca. 7.3). This characteristic offers another mechanism to trigger the response of photosensitizers. Chapter 5 focuses on the exploration of a dual pH- and redox-responsive silicon(IV) phthalocyanine in which the ferrocenyl quenchers are axially coordinated to the macrocycle through an acid-labile hydrazone bond and a reducible disulfide bond. At physiological pH and low level of reducing agent, or under one of these conditions, the linker(s) remain(s) intact and hence the photosensitizer remains photodynamically “inactive“ due to the quenching effect induced by the ferrocenyl unit(s). However, it becomes activated in an environment with low pH and high level of reducing agent, which is analogous to the conditions in tumor tissues. The effects of these external stimuli on the photophysical properties and in vitro photodynamic activities of this novel photosensitizer are examined in this chapter. / Chapter 6 concludes the present study while Chapter 7 reports the experimental procedures. All references adapted in this manuscript are presented in Chapter 8. / ¹H and ¹⁶C{¹H} NMR spectra of all the new compounds are given in the Appendix. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Lau, Ting Fong Janet. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2012. / Includes bibliographical references (leaves 191-202). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese. / Table of Contents --- p.i / Acknowledgment --- p.vi / Abstract --- p.ix / Abstract (in Chinese) --- p.xii / Abbreviations --- p.xiv / List of Figures --- p.xviii / List of Tables --- p.xxvi / List of Schemes --- p.xxviii / Publication Related to This Thesis --- p.xxx / Chapter Chapter 1 --- Introduction / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.1.1 --- History of Photodynamic Therapy --- p.1 / Chapter 1.1.2 --- Basic Principles of Photodynamic Therapy --- p.4 / Chapter 1.1.2.1 --- Photophysical Mechanisms --- p.4 / Chapter 1.1.2.2 --- Biological Mechanisms --- p.7 / Chapter 1.2 --- Photosensitizers of Photodynamic Therapy --- p.8 / Chapter 1.2.1 --- Characteristics of Ideal Photosensitizers --- p.10 / Chapter 1.2.2 --- First-Generation Photosensitizers --- p.11 / Chapter 1.2.3 --- Second-Generation Photosensitizers --- p.13 / Chapter 1.2.4 --- Phthalocyanine-Based Photosensitizers --- p.17 / Chapter 1.3 --- Toward Targeted Photodynamic Therapy --- p.23 / Chapter 1.3.1 --- Site-Specific Delivery of Photosensitizers --- p.23 / Chapter 1.3.1.1 --- Conjugation to Monoclonal Antibodies --- p.23 / Chapter 1.3.1.2 --- Conjugation to Tumor Vessel-Targeted Peptides --- p.24 / Chapter 1.3.1.3 --- Conjugation to Folic Acid --- p.26 / Chapter 1.3.2 --- Activatable Photosensitizers --- p.27 / Chapter 1.3.2.1 --- Environmental Activatable Photosensitizers --- p.27 / Chapter 1.3.2.2 --- Photodynamic Molecular Beacons --- p.30 / Chapter 1.4 --- Dual Chemo- and Photodynamic Therapy --- p.34 / Chapter 1.4.1 --- Covalent Conjugation --- p.34 / Chapter 1.4.2 --- Co-encapsulation in Polymeric Micelles --- p.36 / Chapter 1.4.3 --- Sequential Administration --- p.38 / Chapter 1.5 --- Objectives of This Study --- p.41 / Chapter Chapter 2 --- A Zinc(II) Phthalocyanine Conjugated with an Oxaliplatin Derivative for Dual Chemo- and Photodynamic Therapy / Chapter 2.1 --- Introduction --- p.42 / Chapter 2.2 --- Results and Discussion --- p.43 / Chapter 2.2.1 --- Molecular Design, Synthesis, and Characterization --- p.43 / Chapter 2.2.2 --- Electronic Absorption and Photophysical Properties --- p.46 / Chapter 2.2.3 --- In Vitro Photodynamic Activities --- p.49 / Chapter 2.3 --- Summary --- p.60 / Chapter Chapter 3 --- Zinc(II) Phthalocyanine-Polyamine Conjugates as Efficient Photosensitizers for Photodynamic Therapy / Chapter 3.1 --- Introduction --- p.62 / Chapter 3.2 --- Results and Discussion --- p.64 / Chapter 3.2.1 --- Preparation and Characterization --- p.64 / Chapter 3.2.2 --- Electronic Absorption and Photophysical Properties --- p.68 / Chapter 3.2.3 --- In Vitro Photodynamic Activities --- p.74 / Chapter 3.3 --- Summary --- p.84 / Chapter Chapter 4 --- A Redox-Responsive Silicon(IV) Phthalocyanine for Targeted Photodynamic Therapy / Chapter 4.1 --- Introduction --- p.85 / Chapter 4.2 --- Results and Discussion --- p.86 / Chapter 4.2.1 --- Preparation and Characterization --- p.86 / Chapter 4.2.2 --- Electronic Absorption and Photophysical Properties --- p.89 / Chapter 4.2.3 --- In Vitro Photodynamic Activities --- p.99 / Chapter 4.3 --- Summary --- p.104 / Chapter Chapter 5 --- A Dual pH- and Redox-Responsive Phthalocyanine-Based Photosensitizer for Targeted Photodynamic Therapy / Chapter 5.1 --- Introduction --- p.106 / Chapter 5.2 --- Results and Discussion --- p.107 / Chapter 5.2.1 --- Molecular Design, Synthesis, and Characterization --- p.107 / Chapter 5.2.2 --- Electronic Absorption and Photophysical Properties --- p.115 / Chapter 5.2.3 --- pH- and Redox-Responsive Properties --- p.118 / Chapter 5.2.4 --- In Vitro Photodynamic Activities --- p.130 / Chapter 5.3 --- Summary --- p.136 / Chapter Chapter 6 --- Conclusion and Future Outlook --- p.137 / Chapter Chapter 7 --- Experimental Section / Chapter 7.1 --- General --- p.138 / Chapter 7.1.1 --- Materials and Methods --- p.138 / Chapter 7.1.2 --- Photophysical Measurements --- p.139 / Chapter 7.1.3 --- Cell Lines and Culture Conditions --- p.140 / Chapter 7.1.4 --- Photocytotoxicity Assay --- p.141 / Chapter 7.1.5 --- ROS Measurements --- p.141 / Chapter 7.1.6 --- Intracellular Fluorescence Studies --- p.142 / Chapter 7.1.7 --- Cellular Uptake Determined by an Extraction Method --- p.142 / Chapter 7.1.8 --- Subcellular Localization Studies --- p.143 / Chapter 7.1.9 --- Flow Cytometric Studies --- p.144 / Chapter 7.2 --- Experiments Described in Chapter 2 --- p.145 / Chapter 7.2.1 --- Synthesis --- p.145 / Chapter 7.2.2 --- Photocytotoxicity Assay --- p.155 / Chapter 7.2.3 --- Intracellular Fluorescence Studies --- p.155 / Chapter 7.2.4 --- Cellular Uptake Determined by an Extraction Method --- p.155 / Chapter 7.2.5 --- Subcellular Localization Studies --- p.156 / Chapter 7.3 --- Experiments Described in Chapter 3 --- p.156 / Chapter 7.3.1 --- Synthesis --- p.156 / Chapter 7.3.2 --- Photocytotoxicity Assay --- p.171 / Chapter 7.3.3 --- Effect on Spermidine on the Cellular Uptake --- p.171 / Chapter 7.3.4 --- Intracellular Fluorescence Studies --- p.172 / Chapter 7.3.5 --- Subcellular Localization Studies --- p.172 / Chapter 7.4 --- Experiments Described in Chapter 4 --- p.173 / Chapter 7.4.1 --- Synthesis --- p.173 / Chapter 7.4.2 --- Redox-Responsive Fluorescence Emission Studies --- p.180 / Chapter 7.4.3 --- Redox-Responsive Singlet Oxygen Generation Studies --- p.180 / Chapter 7.4.4 --- Photocytotoxicity Assay --- p.180 / Chapter 7.4.5 --- Intracellular Fluorescence Studies --- p.181 / Chapter 7.4.6 --- Subcellular Localization Studies --- p.181 / Chapter 7.5 --- Experiments Described in Chapter 5 --- p.182 / Chapter 7.5.1 --- Synthesis --- p.182 / Chapter 7.5.2 --- pH- and Redox-Responsive Fluorescence Emission Studies --- p.189 / Chapter 7.5.3 --- pH- and Redox-Responsive Singlet Oxygen Generation Studies --- p.189 / Chapter 7.5.4 --- Intracellular Fluorescence Studies --- p.190 / Chapter Chapter 8 --- References --- p.191 / Chapter Appendix --- ¹H and ¹³C{¹H} NMR Spectra --- p.203
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Novel carboxy and glycosylated phathalocyanines as efficient photosensitizers for photodynamic therapy. / CUHK electronic theses & dissertations collectionJanuary 2007 (has links)
by Choi, Chi Fung. / "September 2007." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references. / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstracts in English and Chinese.
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A mechanistic study on the photodynamic effects of pyropheophorbide-a methyl ester (MPPa) on prostate cancer PC-3MTian, Yuanyuan 01 January 2004 (has links)
No description available.
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Photodynamic therapy in nasopharyngeal carcinoma : mechanistic studies of five photosensitizersYow, Li Miu Ngan 01 January 2003 (has links)
No description available.
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Photodynamic therapy of merocyanine 540 and temoporfin mTHPC on neuroblastoma neuro-2ALee, Yiu Lun 01 January 1999 (has links)
No description available.
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Photosensitizers for photodynamic therapy : uptake into endothelial cells and tissues, and effects on the microvascular structureMenezes da Silva, Fernando Antonio January 1994 (has links)
No description available.
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Photodynamic therapy with haematoporphyrin derivative /Cowled, Prudence Anne. January 1986 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, 1986. / Includes bibliographical references (leaves 159-194).
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