Individuals with NIDDM and low insulin sensitivity (Si) have altered fuel use, which may appear in NIDDM relatives. Adolescent males, six with NIDDM relatives (POS) and six controls (CON), matched for fitness and fatness, participated. Peak oxygen uptake (V02pea), ventilatory threshold (VT) and body composition were measured. Insulin and glucose values were used for Si estimates. Oxygen consumption (V02) and respiratory exchange ratio (RER) measurements were made at rest and during exercise at 80,100 and 120% of VT for substrate use. No group differences were found in anthropometric data, V02pea, VT or blood measurements, although fat free mass (FFM) tended to be greater in CON. CON tended to use a higher percentage carbohydrate and a lower percentage of fat than POS, but differences were no longer present after normalization to FFM. Trends towards fuel use differences may suggest metabolic abnormalities are present in POS, but further investigation is necessary. / School of Physical Education, Sport, and Exercise Science

As more adults with cerebral palsy (CP) are surviving longer (1) an intervention is needed to help reduce spasticity and increase overall strength to improve mobility, and therefore life quality. Adults with CP are lacking a form of independent exercise that allows them to maintain or improve their ambulation skills (1, 2). A new approach to increase muscle strength and flexibility is called whole-body vibration (WBV). The goal of the current study was to determine the acute effects of using an individualized frequency (I-Freq) approach to WBV therapy on gait in adults with CP. In this study, eight adults with CP (age 20-51 years, six men, two women) participated in two sets of testing: the first set was used to determine their I-Freq and the second set to perform a 3D gait analysis before and after a WBV treatment. The WBV was administered in five sets of one minute of vibration followed by one minute of rest. The gait analyses included collection of kinematic and EMG data. Subjects experienced a significant increase in walking speed (P=0.047), stride length (P=0.017) and dynamic ankle range of motion (P=0.042) after the acute bout of WBV. These data show that WBV treatments at I-Freq could help adults with CP maintain their range of motion and overall mobility through an independent and cost effective means. 1. Murphy KP. The adult with cerebral palsy. Orthop Clin North Am2010 Oct;41(4):595-605. 2. Murphy KP, Sobus K, Bliss PM. The Adult with Cerebral Palsy: A Provider-Consumer Perspective. Physical Medicine and Rehabilitation Clinics of North America2009 Aug;20(3):509-+. / School of Physical Education, Sport, and Exercise Science

This study investigated differences in heart rate recovery (HRR) and the underlying autonomic mechanisms following maximal and submaximal exercise between boys and men. Children are known to have a faster HRR than adults. However, the mechanism responsible for these differences is still largely unknown, but is thought to be due to differences in the autonomic nervous system. Ten boys (10.1 ± 0.8 years old) and ten men (24.2 ± 1.3 years old) completed a supine recovery following a maximal and submaximal exercise bout. HRR was measured as the decline in HR during the first minute of recovery and heart rate variability (HRV) was used to measure autonomic function. Boys had a faster HRR during the first minute of recovery from maximal exercise (p<0.05), but there was no difference in HRR following submaximal exercise (p>0.05). The R-R coefficient of variation and high frequency power were both significantly greater following maximal exercise in boys than men. There were no differences in HRV variables following submaximal exercise. These findings suggest that faster parasympathetic re-activation could be a mechanism responsible for faster HRR in boys compared to men, especially following maximal exercise. / School of Physical Education, Sport, and Exercise Science

This investigation evaluated the Influence of beverage carbonation and carbohydrate content on fluid replacement following exercise/thermal dehydration. Eight recreationally trained men cycled at 50% of their maximal aerobic power in a hot environment (40°C 40% relative humidity) until a body weight loss of 4.12 ± 0.02% was attained. In the subsequent four hours subjects ingested one of four randomly assigned solutions at 15 minute intervals. The total volume ingested equalled that lost during dehydration. The solutions varied in their carbohydrate and carbonation content as follows: 1) CK: carbonated 10% carbohydrate solution, 2) NCK: non-carbonated 10% carbohydrate solution, 3) CNK: carbonated non-caloric solution, and 4) NCNK: non-carbonated non-caloric solution. Before dehydration and at 0, 60, 120, 180, and 240 minutes of recovery subjects performed a five minute cycling bout (SCB) at 70% venous blood samples were obtained before and after dehydration, and at 30, 90, 150, and 240 minutes of of their maximal aerobic power. In addition, resting data from the carbohydrate treatments were pooled and recovery. Rehydration evaluated from body weight, plasma volume, plasma protein, and urine volume data was not significantly (p> 0.05) different between treatments at any time. In addition, carbonated beverage (CK and CNK) ingestion did not significantly alter the pC02 or pH of venous blood at rest or the respiratory exchange ratio (RER) during exercise, however, the ingestion of carbohydrate beverages (CK and NCK) elevated the RER during the SCB relative to the level elicited by Ingestion of non-carbohydrate beverages. Oxygen consumption during the SCB at 240 minutes was significantly (p< 0.05) higher in the non-carbohydrate treatments (NCNK vs. CK and CNK and NCNK vs. NCK), however, no differences in caloric expenditure were observed. Arterialized blood lactate one minute after each SCB was not different between treatments. Lactate levels were significantly higher, however, when compared to the non-carbohydrate treatments. No significant differences were observed in ratings of perceived exertion, or ratings of gastrointestinal comfort. The results of this Investigation suggest that solutions which are carbonated and/or contain a carbohydrate concentration of 10 b are as effective as non-carbonated and/or non-carbohydrate solutions with regard to fluid replacement. In addition, the ingestion of carbonated beverages does not appear to be associated with alterations in the pC02 or pH of venous blood or gastric distress relative to the levels attained with non-carbonated beverages. / School of Physical Education

Eight well-trained male and female cyclists were studied to determine the effect of sodium and/or water intake on plasma aldosterone during six hours of cycling (55% VO2max) in a warm environment (Tdb = 350C WBGT = 30°C). Each subject randomly completed three trials (water = W; saline = S and no fluid = NF) at one week intervals. Venous blood samples were obtained before dehydration, at 2, 4, 5 and 6 hours during exercise, and also after dehydration. Plasma samples were analyzed for hemoglobin, sodium, potassium, aldosterone and osmolality. Sweat and urine samples were also collected and analyzed for sodium content. Plasma volume based on hemoglobin decreased significantly ( P < 0.01 ) at 15 min in all three trials (Trial W = -7.6%±1.12%; Trial S = -8.6%±1.42% and Trial NF = -6.7%±0.88%) and continued to decrease significantly in Trial NF during exercise ( -10.99% ± 1.3% at the 2th hr; -15.5% ±1.3% at the 4th hr and -16.8%±1.32% at the 5th hr).No significant differences were found betwee trials. Plasma sodium concentration [Na+] decreased over time in Trials W and S and increased in Trial NF due to plasma volume loss. Significant differences in [ Na+ ] were found between Trial NF and Trials W or S. Plasma sodium [Na+ ] adjusted by plasma volume change decreased significantly at 2 hours ( P < 0.01) in the three trials. Average total sodium content of plasma decreased by 125.9 mEq during Trial S, 223.1 mEq during Trial W and 147.1 mEq during Trial NF. Plasma potassium increased significantly (P < 0.01) at 2 hours in all three trials. Plasma osmolality increased significantly (P < 0.01) during prolonged exercise (Trial W = 287.1±2.4 mEq/l; Trial S = 289.4±1.17 mEq/1 and Trial NF = 306±1.6 mEq/1). No significant differences were found between Trials W and S although osmolality was lower in Trial W than in Trial S. A significant difference in osmolality was obtained between Trial NF and Trials W and S (P < 0.01). Plasma aldosterone increased significantly (P < 0.01) during exercise and decreased after exercise. No significant differences existed between Trials W and S although aldosterone levels were lower in Trial S than in Trial W. However, a significant difference was found between Trial NF and Trials W or S. The results of this study suggest that plasma aldosterone has an inverse relationship with plasma volume changes and total sodium concentrations. An increase in plasma potassium and a decrease in plasma sodium during prolonged exercise in a warm environment significantly enhanced plasma aldosterone concentration. The intake of water significantly decreased plasma aldosterone during prolonged exercise in a warm environment, but the intake of sodium had no significant effect in this study. / School of Physical Education

The purpose of this study was to compare the exercise blood pressure response of subjects with a history of exercise induced hypertension between the submaximal 3-3-3 exercise test and a maximal Bruce treadmill protocol. Seven male and four female subjects with a mean age of 49 ± 2 years were recruited for the study. All subjects were selected based on a previous elevation of exercise diastolic blood pressure >_ 10 mmHg during maximal exercise.The 3-3-3 test is a short, standardized treadmill test protocol that consists of one stage of treadmill walking at a 3% grade, at 3 miles per hour, for 3 minutes. Subjects completed the 3-3-3 test on one day and the Bruce protocol on a different day with 24-48 hours between tests. Test administration order was randomized and all tests were done at the same time of the morning with all blood pressures taken by the same technician with a mercury manometer.The increase in diastolic pressure from rest was significantly lower (p<0.004) during the 3-3-3 protocol compared to the peak of the Bruce protocol. There were no statistical differences between the change in diastolic pressure from rest to peak exercise when the 3-33 protocol was compared to the Bruce Stage I or when the Bruce Stage I was compared to the peak Bruce diastolic measurement. The mean change in diastolic pressure from rest to peak exercise in the3-3-3 protocol was 3.4 ± 2.6 mmHg as compared to 9.9 ± 2.0 mmHg for the Bruce Stage I, and 15.4 ± 2.4 mmHg from rest to peak exercise with the peak Bruce. There were no differences in resting or standing systolic or diastolic blood pressure values between protocols done on separate trial days. The change in systolic response from rest to peak exercise and the peak systolic pressures were significantly different (p<0.001) between the Bruce Stage I and peak Bruce and between the 3-3-3 and peak Bruce. There were no significant differences in the heart rate and rate pressure product between the third minute of the 3-3-3 protocol and the third minute of the Bruce Stage I (p<0.001).The 3-3-3 test was not able to elicit the same rise in diastolic blood pressure as the Bruce treadmill test in this population, although the Bruce Stage I was able to elicit a response that was not statistically different than the response of the peak Bruce protocol. This implies that submaximal treadmill testing may be a feasible method to screen for the presence of an exaggerated diastolic blood pressure response to exercise. / School of Physical Education

The purpose of this investigation was to determine the reliability and validity of VO2max estimates from the ACSM submaximal cycle ergometer test compared to a maximal cycle ergometer test. Thirty healthy male and female subjects aged 21-54 volunteered for this study. Each subject performed a maximal cycle ergometer test and two ACSM submaximal cycle ergometer tests. At least 48 hours elapsed between the maximal test and the first submaximal test while at least 24 hours elapsed between the two submaximal tests. Heart rates were recorded from a radio telemetry monitor and from 15 second palpation of the radial artery. A trained technician performed all palpations and was blinded from the telemetry monitor during testing to eliminate any bias. Maximal oxygen uptake (VO2max) was estimated by extrapolatingrate for each submaximal trial. To determine inter-test radio telemetry monitor (MON), to an age predicted maximal heart reliability between submaximal trials, correlational analysis andt-test were performed. A correlation of r= .863 and r= .767 were found between the two trials for MON V02max and PALP VO2max respectively. Paired t-test revealed no significant difference (p< .01) between MON V02max 1 and MON VO2max 2 or between PALP V02max 1 and PALP VO2max 2. Although there were no significant differences between trials, the mean data was often not indicative of the individual data since there were 8 MON VO2max cases and 19 PALP VO2max cases where VO2max estimates varied by > 0.5 L/min. There were significant differences between all V02max estimates when compared to measured VO2max and mean percent error in estimates for MON VO2max and PALP VO2max were 25.7% and 33.8%, respectively. The mean error of MON VO2max 1 and MON VO2max 2 were .61 and .63 L/min, respectively. The mean error for PALP VO2max 1 and PALP VO2max 2 were somewhat higher at .90 and .73 L/min, respectively. The present data indicates that a large error is present in VO2max estimates between trials and between measured and estimated VO2max. Therefore, caution must be used when interpreting VO2max estimates from the ACSM submaximal cycle ergometer test. / School of Physical Education

The purpose of this study was to examine if measurements of submaximal blood lactate may improve the prediction of VO2max from submaximal exercise tests. Subjects (39 men & 21 women, age 20-44 yrs) completed a branching protocol on a cycle ergometer which consisted of 3 submaximal stages of 4 minutes each followed by a maximal effort. During the last minute of each stage heart rate (HR) was recorded from telemetry and a capillary blood sample was obtained from a finger puncture. Maximal power output was estimated from the extrapolation of the 3 submaximal HRs to age predicted HRmax and blood lactate concentration was measured using a Yellow Springs analyzer (Yellow Springs, OH). Data were analyzed using multiple regression procedures. Estimated maximal power output (238 ± 56 W) was significantly related to measured VOzmaX (p < 0.001, r2= .523). Mean values for ACSM estimated, ACSM-revised and measured VOz.X were not significantly different (3.170 vs. 3.140 vs. 3.260 L/min respectively), however the SEE % for the ACSM and ACSM revised estimated VOz,. were 13.4% and 12.1%, respectively. When the change in power output relative to the change in blood lactate concentrations (po/BL) from stage 1 to stage 2 and the estimated maximal power output from 3 submaximal heart rates were added as independent variables to the regression model with measured VO2,t as the dependent variable, po/BL was significantly correlated with VO X , accounting for 7.6% of the variance in VOz... In conclusion, only the relative change in power output to the relative change in blood lactate measure from stage 1 to _2 were significant in improving the estimation of VO2 during submaximal exercise tests using a branching protocol. Other blood lactate measures did not improve the estimation of VOA,. in this study. / School of Physical Education

The purpose of the present study was to examine the relationship between exercise induced muscle damage and growth factors during two different modes of exercise. Nine healthy untrained male subjects participated in this study and performed two separate single bouts of isokinetic concentric (Con) and eccentric (Ecc) leg extension exercise on the CYBEX NORMT°". The workload was maintained at 75% of 1 RM for each trial, respectively. The maximum sets of 10 repetitions were performed during the Con trial, and the number was also duplicated during the Ecc trial, with 40 seconds of rest between sets. Serum levels of hGH, creatine kinase (CK), and lactic acid were measured, and the CK level was used to determine the degree of muscle tissue damage. A muscle soreness questionnaire was provided to the subjects to assess the degree of quadriceps muscle soreness following each trial. The EMG activity of the rectus femoris and vastus medialis muscles was recorded during each trial. The results of the present study demonstrated no significant differences in hGH output and CK activity between the exercise trials, although there was a significant different lactic acid response (P < 0.05). However, the Con trial produced significant increases (P < 0.05) in hGH and CK levels above the resting value at the post-exercise times. In fact, the 75% Con trial conducted in this study induced an increase in hGH release (peak: 8.23 ± 3.21 ng/ml) that was 2 X higher than a 120% Ecc trial (peak: 3.8 ± 1.2 ng/mI) of the prior study. The results of the present study demonstrate that a single bout of Con resistance exercise at the same intensity (75% of 1 RM), angular velocity, and ROM as a single bout of Ecc exercise can produce greater increases in hGH output and CK response than its Ecc counterpart. This finding does not support the previous results from this laboratory, showing that Ecc exercise is a stronger promoter of hGH output. However, it suggests that the amount of work performed is an important factor for hGH release because the exercise volume applied in the present study was greater than that of the prior study. The CK response of the subjects in this study, as well as the previous work indicate that hGH output is also dependent on exercise that elicits muscle damage. Therefore, the results of the present study suggest that the mode of exercise, Con vs. Ecc, is not as important as the stress placed on the exercising muscle in order to induce optimal muscle hypertrophy. / School of Physical Education

Background: Normative values of VO2max have been developed or updated based on the estimated VO2max, but measured normative values of VO2max have not been developed yet. VO2max has been reported to relate to coronary heart disease (CHD) risk factors, yet most of the studies have used estimated VO2max to compare CHD risk factors. Therefore, the purpose of this study was to develop norms for VO2max from the Ball State University (BSU) Adult Physical Fitness Program cohort that represented percentiles based on the measured VO2max. In addition, this study evaluated the relationship between measured VO2max and six coronary heart disease (CHD) risk factors, which include Body Bass Index (BMI), high density lipoprotein cholesterol (HDL-C), glucose, triglyceride (TG), total cholesterol (TC) and resting blood pressure (BP).Methods: Subjects were healthy men (N=1,867) and women (N=1,253), ranging in age from 19 to 75 years, who completed the standard BSU Adult Physical Fitness Program quiet and exercise testing sessions between 1971 and 2000, with the graded exercise testing (GXT) conducted with one of the following protocols including modified walking, running, Balke, Bruce, and BSU/Bruce ramp. To be included, subjects had to achieve respiratory exchange ratio (RER) >1.0 during their exercise test.Results: All subjects were classified into ten group determined by deciles of VO2max for each decade of age for males and females respectively. A linear regression showed that VO2max decreased 10.1% per decade (0.44 mi.kg'•min'•yr') for men and 9.7% per decade (0.32 ml•kg-l.min-l.yr') for women. There was no significant difference in the rate of agerelated decline in VO2max per decade between men and women or between deciles of VO2max. Also, the percent of subjects with an exercise history code >5 (regularly participate in exercise at least 3 days per week) was higher in the higher VO2max deciles. In addition, five positive CHD risk factors were inversely related to VO2max, and one negative CHD risk factor was directly related to VO2max. As expected, the higher CRF groups had a more favorable CHD risk factor profile. Also, the mean of VO2max decreased with the greater number of CHD risk factors.Conclusion: This study developed normative values of the VO2max based on measured VO2max. VO2max was significantly correlated to CHD risk factors. / School of Physical Education, Sport, and Exercise Science