The KwaZulu-Natal Bight is an important area along the South African east coast, stretching 160 km north from Scottsburgh to St Lucia (Lutjeharms et al., 2000). The Bight is of interest to the region as the area contains some distinct physical features, which are presumed to drive the ecological functioning of the shelf ecosystem through their role in nutrient sources. These include the Tugela River, the second largest river in South Africa in terms of outflow, and the Agulhas Current that forms an outer border at the edge of the continental shelf. Phytoplankton interacts with the majority of essential ecological networks and therefore greatly influences marine ecosystems. To this end, it is necessary to understand their ecophysiological rate processes – particularly those that are influenced by the dominant nutrient inputs to the Bight. The overall aim of this project is therefore to provide an insight into the sources of nutrients driving phytoplankton productivity in the Bight. Synoptic surveys were conducted to provide an indication of the distribution of Total Suspended Solids (TSS), Particulate Organic Matter (POM) and phytoplankton in the Bight, while focussed experiments used stable isotopes to examine the rate processes involving C and N acquisition, as well as sources of N available in the surface water. Concentration of particulate organic phosphorus and nitrogen were found to be higher in the wet season when compared to the dry season. During the wet season a large variation in chlorophyll-a fluorescence was observed across the Bight, while natural abundance isotope data indicated a seasonal change in the nutrient source available. For the wet season nutrient concentration varied with site and depth, however uptake rates (μg N.1ˉ¹.hˉ¹) measured using ¹⁵N tracer additions were not significantly different with site and depth. Alternatively, the dry season showed a significant difference between site in surface waters. In the wet season the mid shelf area had the highest uptake rate and phytoplankton biomass while the Richards Bay north site dominated, with regard to the previously mentioned factors, in the dry season. At the time of the experiments, neither the Durban eddy nor the upwelling cell were present, and hypotheses regarding the importance of these physical features in driving phytoplankton nutrient acquisition could not be assessed. However, a notable difference in uptake rate between the wet and dry seasons was observed, and this difference is likely due to the fluvial sources of nutrients from the Tugela and many other rivers entering the KZN coast, which are absent during the dry season. The results indicate that terrestrial nutrient sources play a major role in influencing nutrient concentrations on the Bight, and hence influence the nearshore ecosystem of the region. / Thesis (M.Sc.)-University of KwaZulu-Natal, Westville, 2012.

Mechanisms controlling the productivity, abundançe and taxonomic composition of phytoplankton in tropical Lake Malawi were examined by monitoring phytoplankton dynamics in 8 regions covering the length of the lake (560 km) over a 10-12 month period, and relating these dynamics to spatio-temporal changes in thermal structure, nutrient availability, and meteorological conditions. In addition, nearshore benthic photosynthetic rates were measured in 7 different months.Spatial and temporal changes in areal photosynthetic rates were due almost entirely to changes in the efficiency of light utilization by the phyroplankton community. An evaluation of potential factors which might influence tight utilization indicates that nutrient availabitity is the most important. Most of the photosynthetic N and P demand is met by internal recycling within the upper 200 m, and therefore spatio-temporal variation of phytoplankton photosynthetic rate is closely related to changes in mixing regime. It is shown that the dominant meteorological factors responsible for changes in the mixing regime were solar radiation and windspeed. A comparison with previous photosynthesis data for Lake Malawi indicates that windspeed is a dominant factor controlling interannual variability. Shallow areas of the lake were more productive than deep areas, due to more intense upwelling and more efficient internal nutrient recycling in shallow waters. Within the littoral zone, benthic photosynthetic rates were very high, accounting for 14% to 28% of total net photosynthesis within the shallow southeast arm. Phytoplankton biomass was not correlated with photosynthetic rate, indicating that biomass loss processes were important in controlling biomass variability. Changes in phytoplankton taxonomic composition were related to changes in mixing regime. Cyanobacteria and chlorophytes were dominant throughout much of the study period, but diatoms made up a significant proportion of total biomass during periods of increased turbulence and nutrient availability. An analysis of phytoplankton surface area : volume ratios revealed that organism shape and size are important determinants in species succession. Previous studies have emphasized the low variability of phytoplankton biomass and photosynthetic rates in tropical lakes, relative to temperate lakes. An inter-lake comparison reveals that this tenet does not apply to large lakes. Fluctuations in the mixing regime of large tropical lakes have an effect on phytoplankton variability similar in magnitude to the effect of fluctuating solar irradiance in large temperate lakes.

Planktono bendrijos tyrimai teikia esminę informaciją vertinant ežerų ekologinę būklę ir jų raidos tendencijas, nes planktono dumbliai yra pagrindiniai, o dažnai ir vieninteliai pirminės organinės medžiagos producentai, o zooplanktonas yra grandis tarp pirminių producentų ir aukštesnių mitybos lygmenų pelaginėje mitybos grandinėje. Vertingiausi ekologiniu pažiūriu yra mezotrofiniai Lietuvos ežerai. Juos pagal zooplanktono bendrijas galima suskirstyti į 3 (charakteringas) grupes: ežerus, kuriuose gyvena irklakojis vėžiagyvis Limnocalanus macrurus, ežerus apgyvendintus kito relikto Eurytemora lacustris ir ežerus be ledynmečio reliktinių planktono vėžiagyvių. Informacija apie šių ežerų grupėse vykstančius procesus teikia galimybę vertinti Lietuvos mezotrofinių ežerų raidos tendencijas. Darbo tikslas – nustatyti charakteringų mezotrofinių Lietuvos ežerų planktono dumblių ir vėžiagyvių bendrijų struktūrą ir kaitą, palyginti ežerų grupių planktono bendrijas ir įvertinti Lietuvos mezotrofinių ežerų kitimo tendencijas. Darbe atlikti kompleksiniai mezotrofinių ežerų fitoplanktono ir zooplanktono struktūros tyrimai. Šie duomenys įvertina dabartinę Lietuvos mezotrofinių ežerų būklę ir jos raidos tendencijas, bei papildo informaciją apie mezotrofinių ežerų fitoplanktoną ir zooplanktoną. Pirmą kartą Lietuvoje atlikti dumblių ramybės stadijų aktyvacijos veiksnių tyrimai. Tirtuose ežeruose apibūdintos 32 anksčiau Lietuvoje neregistruotos dumblių ir melsvabakterių rūšys. / Studies of plankton community provide vital information in assessing the ecological status of lakes and their evolution. Phytoplankton is essential, and often the only primary producer of organic matter. Zooplankton takes an important place in the pelagic food chain because of the link between the primary producers and higher trophic levels. The most valuable are Lithuanian mesotrophic lakes. Depending on zooplankton communities, those lakes can be divided in 3 (characteristic) groups: lakes with glacial relict calanoids Limnocalanus macrurus, lakes with another calanoids Eurytemora lacustris and lakes without relict crustaceans. The information obtained after examination of several lakes from different groups, is useful for evaluating the status of Lithuanian mesotrophic lakes. The aim of the present study was to identify defining characteristics of phytoplankton and zooplankton community structure and change in Lithuanian mesotrophic lakes, compare plankton communities and to assess change trends of Lithuanian mesotrophic lakes. In this study complex analyses of phytoplankton and zooplankton structure were performing in mesotrophic lakes. Our data assess the current state of Lithuanian mesotrophic lakes and complement the information about phytoplankton and zooplankton in mesotrophic lakes. The obtained results are important for evaluation of the ecological status of the lakes and providing the environmental control tools to ensure good ecological status of the lakes. For... [to full text]

Studies of plankton community provide vital information in assessing the ecological status of lakes and their evolution. Phytoplankton is essential, and often the only primary producer of organic matter. Zooplankton takes an important place in the pelagic food chain because of the link between the primary producers and higher trophic levels. The most valuable are Lithuanian mesotrophic lakes. Depending on zooplankton communities, those lakes can be divided in 3 (characteristic) groups: lakes with glacial relict calanoids Limnocalanus macrurus, lakes with another calanoids Eurytemora lacustris and lakes without relict crustaceans. The information obtained after examination of several lakes from different groups, is useful for evaluating the status of Lithuanian mesotrophic lakes. The aim of the present study was to identify defining characteristics of phytoplankton and zooplankton community structure and change in Lithuanian mesotrophic lakes, compare plankton communities and to assess change trends of Lithuanian mesotrophic lakes. In this study complex analyses of phytoplankton and zooplankton structure were performing in mesotrophic lakes. Our data assess the current state of Lithuanian mesotrophic lakes and complement the information about phytoplankton and zooplankton in mesotrophic lakes. The obtained results are important for evaluation of the ecological status of the lakes and providing the environmental control tools to ensure good ecological status of the lakes. For... [to full text] / Planktono bendrijos tyrimai teikia esminę informaciją vertinant ežerų ekologinę būklę ir jų raidos tendencijas, nes planktono dumbliai yra pagrindiniai, o dažnai ir vieninteliai pirminės organinės medžiagos producentai, o zooplanktonas yra grandis tarp pirminių producentų ir aukštesnių mitybos lygmenų pelaginėje mitybos grandinėje. Vertingiausi ekologiniu pažiūriu yra mezotrofiniai Lietuvos ežerai. Juos pagal zooplanktono bendrijas galima suskirstyti į 3 (charakteringas) grupes: ežerus, kuriuose gyvena irklakojis vėžiagyvis Limnocalanus macrurus, ežerus apgyvendintus kito relikto Eurytemora lacustris ir ežerus be ledynmečio reliktinių planktono vėžiagyvių. Informacija apie šių ežerų grupėse vykstančius procesus teikia galimybę vertinti Lietuvos mezotrofinių ežerų raidos tendencijas. Darbo tikslas – nustatyti charakteringų mezotrofinių Lietuvos ežerų planktono dumblių ir vėžiagyvių bendrijų struktūrą ir kaitą, palyginti ežerų grupių planktono bendrijas ir įvertinti Lietuvos mezotrofinių ežerų kitimo tendencijas. Darbe atlikti kompleksiniai mezotrofinių ežerų fitoplanktono ir zooplanktono struktūros tyrimai. Šie duomenys įvertina dabartinę Lietuvos mezotrofinių ežerų būklę ir jos raidos tendencijas, bei papildo informaciją apie mezotrofinių ežerų fitoplanktoną ir zooplanktoną. Pirmą kartą Lietuvoje atlikti dumblių ramybės stadijų aktyvacijos veiksnių tyrimai. Tirtuose ežeruose apibūdintos 32 anksčiau Lietuvoje neregistruotos dumblių ir melsvabakterių rūšys.

Marine productivity along the western Antarctic Peninsula (WAP) is declining. The WAP is site of the fastest regional warming in the southern hemisphere, and has experienced atmospheric and oceanic temperature increases leading to increased glacial inputs and reduced winter sea-ice cover. Sea-ice is a key link between climate and phytoplankton production, as melting sea-ice stratifies the water column and provides a source of micronutrients to surface waters. Reductions in ice cover have been accompanied by declining chlorophyll (chl; a proxy for phytoplankton biomass), and a shift to smaller cell sizes in phytoplankton communities. These reductions have implications for carbon drawdown and production available to higher trophic levels. However, little is known about phytoplankton shifts at the community level, as existing studies are based on satellite records and photosynthetic pigment analyses. To elucidate the nature of the changes within phytoplankton assemblages, high-resolution time-series data of diatom speciation are coupled to environmental data from five years in Ryder Bay (Adelaide Island, WAP). Long-term monitoring at this site by the British Antarctic Survey has identified a strong relationship between chl and water column stratification, and this study spans a wide range of physical conditions and biological production. By comparing high- and low-chl phytoplankton assemblages, this study investigates the mechanisms underlying productivity changes and the manner in which these changes impact nutrient cycling, drawdown and trophic transfer. The results presented here are the first full season in-situ records documenting differences in phytoplankton and diatom assemblages between highand low-chl years. The primary difference between chl conditions is a dramatic decline in diatom abundance. This analysis indicates that the mechanism producing low-chl seasons is less stratified surface waters, where light levels are much more variable than in high-chl years. Overall production is reduced, and small increases are seen in biomass of prymnesiophytes, which are better adapted to variable light. These shifts in phytoplankton composition and size structure are consistent with a southward propagation of observed climate change effects. Within the diatom community, changes in seasonal succession and a decrease in species richness occurred following low winter sea-ice. As the main component of high productivity and that most efficiently transferred to higher trophic levels, variation in diatom production due to environmental conditions is a mechanism to explain the observed WAP ecosystem changes and chl decline. Changes in phytoplankton stocks and composition also affect nutrient use, and here the use of silicon and iron (Si and Fe, respectively, which limit productivity in large areas of the Southern Ocean) is investigated. Seasonal Si budgets estimated from Si isotopes indicate a 40 – 70% decline in Si use between high-chl and intermediate-chl years, in agreement with other indices of productivity. The consequences of reduced demand and changing supply suggest future accumulation of Si in WAP surface waters. This should increase Si export away from the WAP shelf, which may act as a mechanism to enhance productivity and carbon drawdown in the wider Southern Ocean. Sources of Fe were assessed by direct measurement and naturally occurring radioisotopes of radium. These reveal significant inputs at the surface (due to glacial sources) and to deep waters (from shelf/slope sediments), which dominate supply to the surface mixed layer at different times. Iron availability and nutrient drawdown indicate that Fe is supplied to WAP surface waters in excess of biological demand. Projected changes to Fe sources and sinks indicate that continued warming will increase the WAP Fe inventory. As for Si, this excess Fe may also be advected away from the shelf, making this region a net Fe source to the Southern Ocean.

The western Antarctic Peninsula (WAP) is a hotspot of climatic and oceanographic change, with a 6°C rise in winter atmospheric temperatures and >1°C warming of the surface ocean since the 1950s. These trends are having a profound impact on the physical environment at the WAP, with widespread glacial retreat, a 40% decline in sea ice coverage and intensification of deep water upwelling. The main objective of this study is to assess the response of phytoplankton productivity to these changes, and implications for the marine carbon and nitrogen cycles in the WAP coastal zone. An extensive suite of biogeochemical and physical oceanographic data was collected over five austral summer growing seasons in northern Marguerite Bay between 2004 and 2010. Concentrations and isotopic compositions ( 15N, 13C, 14C) of dissolved nitrate, dissolved inorganic carbon species, particulate nitrogen, organic carbon and chlorophyll a are used in the context of a substantial ancillary dataset to investigate nutrient supply, phytoplankton productivity and nutrient uptake, export flux and the fate of organic material, and the factors underpinning pronounced seasonal and interannual variability. High-resolution biogeochemical time-series data for surface and underlying seawater, sea ice brine, sediment trap material and coretop sediments allow detailed examination of carbon and nitrogen cycle processes under contrasting oceanographic conditions and the interaction between these marine processes and air-sea exchange of climate-relevant CO2. This study shows that the WAP marine environment is currently a summertime sink for atmospheric CO2 in most years due to high productivity and biological carbon uptake sufficient to offset the CO2 supply from circumpolar deep waters, which act as a persistent source of heat, nutrients and CO2 across the shelf. For the first time, CO2 sink/source behaviour is parameterised in terms of nitrate utilisation, by exploiting the relationship between CO2 and nitrate concentrations, and deriving the nitrate depletion at which surface ocean CO2 is undersaturated relative to atmosphere and carbon sink behaviour is achieved. This could have vast utility in examining CO2 sink/source dynamics over greater spatial and temporal scales than by direct CO2 measurements, of which availability is more limited. This study documents abrupt changes in phytoplankton productivity, nitrate utilisation and biological CO2 uptake during a period of rapid sea ice decline. In fact, nitrate utilisation, particulate organic matter production and biological CO2 uptake all decrease by at least 50 % between a sea ice-influenced, high productivity season and one of low sea ice and low productivity. The key driver of interannual variability in production and export of organic material is found to be upper ocean stratification and its regulation of light availability to phytoplankton. Productivity, CO2 uptake and export are maximal when stratification is sufficient to provide a stable well-lit surface environment for phytoplankton growth, but with some degree of mixing to promote export of suspended organic matter. Strong stratification causes intense initial production, but retention of suspended organic particles in the surface ocean induces a self-shading effect, and overall productivity, CO2 uptake and export fluxes are low. When stratification is weak, mixing of phytoplankton over a larger depth range exposes cells to a wider range of light levels and reduces photosynthetic efficiency, thus total productivity and CO2 uptake. A conceptual model is developed here, which attempts to describe the mechanism by which sea ice dynamics exert the principal control on stratification and therefore productivity and CO2 uptake at the WAP, with potential application to other regions of the Antarctic continental shelf. Although meteoric waters (glacial melt and precipitation) are more prevalent in surface waters throughout the study, sea ice meltwater variability is driven by large and rapid spring/early summer pulses, which stabilise the upper ocean and initiate phytoplankton growth. The timing and magnitude of these sea ice melt pulses then exert the key control on stratification and seasonal productivity. In a low sea ice year of this study, the sea ice trigger mechanism was absent and productivity was low. This strongly suggests that ongoing sea ice decline at the WAP and greater frequency of such low sea ice years is likely to drive a dramatic reduction in productivity and export, which would substantially reduce the capacity of the summertime CO2 sink in this region. Ongoing warming and ecosystem change are thus likely to have severe impacts on net CO2 sink/source behaviour at the WAP over the annual cycle, and the role of the Southern Ocean in regulating atmospheric CO2 and global climate. Finally, factors influencing the stable isotopic signature of particulate organic carbon ( 13CPOC), a common paleo-proxy, are assessed. 13CPOC is greatly influenced by seasonal shifts in diatom assemblages and isotopically heavy sea ice material, so cannot be used as a robust proxy for ambient CO2 in the coastal Southern Ocean.

I investigated the usefulness of phytoplankton for the removal of surface water contaminants. Three experiments, consisting of nine large mesocosms (92.2 m3) were suspended in the flooded DJX uranium pit at Cluff Lake (Saskatchewan, Canada), and filled with contaminated mine water. During the summer of 2003, each mesocosm was fertilized with a different amount of phosphorus throughout the 35 day experiment to stimulate phytoplankton growth, and to create a range in phosphorus load (g) to examine how contaminants may be affected by different nutrient regimes. Algal growth was rapid in fertilized mesocosms as demonstrated by chlorophyll a profiles. As phosphorus loads increased there were significant declines in the surface water concentrations of As, Co, Cu, Mn, Ni, and Zn. This decline was near significant for uranium. The surface water concentrations of Ra226, Mo, and Se showed no relationship to phosphorus load. Contaminant concentrations in sediment traps suspended at the bottom of each mesocosm generally showed the opposite trend to that observed in the surface water, with most contaminants (As, Co, Cu, Mn, Ni, Ra226, U, and Zn) exhibiting a significant positive relationship (P < 0.05) with phosphorus load. Sediment trap concentration of Se and Mo did not respond to nutrient treatments. Similar experiments were repeated during the mid- and late-summer of 2004, with 5 mesocosms being fertilized with phosphorus, and another 4 with both phosphorus and ammonium to create different nutrient gradients. Results from these experiments were much more variable than those seen in the experiment conducted in 2003, and small samples (n = 5 for phosphorus treatments and n = 4 for both phosphorus and ammonium treatments) yielded insufficient statistical power to effectively determine statistically significant trends. However, contaminant sedimentation tended to respond to phosphorus treatments in a similar manner as results from 2003; phosphorus-with-ammonium treatments had little positive effect on contaminant sedimentation rates. My results suggest that phytoremediation has the potential to lower many surface water contaminants through the sedimentation of phytoplankton. Based on our results from 2003, we estimate that the Saskatchewan Surface Water Quality Objectives (SSWQO) for the DJX pit would be met in approximately 45 weeks for Co, 65 weeks for Ni, 15 weeks for U, and 5 weeks for Zn if treated using phytoremediation.Note:Appendix A content (pages 92-95) contains copyrighted material which has been removed. It can be viewed in the original thesis upon request.

Mechanisms controlling the productivity, abundançe and taxonomic composition of phytoplankton in tropical Lake Malawi were examined by monitoring phytoplankton dynamics in 8 regions covering the length of the lake (560 km) over a 10-12 month period, and relating these dynamics to spatio-temporal changes in thermal structure, nutrient availability, and meteorological conditions. In addition, nearshore benthic photosynthetic rates were measured in 7 different months.Spatial and temporal changes in areal photosynthetic rates were due almost entirely to changes in the efficiency of light utilization by the phyroplankton community. An evaluation of potential factors which might influence tight utilization indicates that nutrient availabitity is the most important. Most of the photosynthetic N and P demand is met by internal recycling within the upper 200 m, and therefore spatio-temporal variation of phytoplankton photosynthetic rate is closely related to changes in mixing regime. It is shown that the dominant meteorological factors responsible for changes in the mixing regime were solar radiation and windspeed. A comparison with previous photosynthesis data for Lake Malawi indicates that windspeed is a dominant factor controlling interannual variability. Shallow areas of the lake were more productive than deep areas, due to more intense upwelling and more efficient internal nutrient recycling in shallow waters. Within the littoral zone, benthic photosynthetic rates were very high, accounting for 14% to 28% of total net photosynthesis within the shallow southeast arm. Phytoplankton biomass was not correlated with photosynthetic rate, indicating that biomass loss processes were important in controlling biomass variability. Changes in phytoplankton taxonomic composition were related to changes in mixing regime. Cyanobacteria and chlorophytes were dominant throughout much of the study period, but diatoms made up a significant proportion of total biomass during periods of increased turbulence and nutrient availability. An analysis of phytoplankton surface area : volume ratios revealed that organism shape and size are important determinants in species succession. Previous studies have emphasized the low variability of phytoplankton biomass and photosynthetic rates in tropical lakes, relative to temperate lakes. An inter-lake comparison reveals that this tenet does not apply to large lakes. Fluctuations in the mixing regime of large tropical lakes have an effect on phytoplankton variability similar in magnitude to the effect of fluctuating solar irradiance in large temperate lakes.

A mesocosm experiment with four triplicated treatments (control, clear carbon, pigment and dissolved organic matter) was carried out to investigate the effects of light (by pigment addition) and carbon (C) on phytoplankton communities and on the grazing pressure of microzooplankton on phytoplankton in the Baltic Sea. Phytoplankton concentration and species composition were determined by using an inverted microscope following the Utermöhl method. In order to measure differences in phyto-zooplankton trophic interactions between treatments, a dilution experiment was performed at the end of the mesocosm experiment. Surprisingly, the results show that light attenuation was beneficial to phytoplankton production while carbon enrichment had negative effects on phytoplankton production, the highest phytoplankton production was in the low light treatment and lowest phytoplankton production was in the clear C treatment. Cyanobacteria were the most dominant group, representing over 95% of the phytoplankton community. Diatoms were the least dominant group, representing less than 5% of the phytoplankton community. Microzooplankton grazing pressure was affected by light attenuation and C enrichment. Grazing pressure was highest on cyanobacteria in the clear C treatment, but chrysophytes experienced low grazing pressure and had high growth rates in the Control treatment where microzooplankton fed selectively on chrysophytes. The least abundant group, diatoms, decreased although they experienced no grazing pressure in the Control and clear C treatments. This experiment resulted in two surprising findings. First, increased light intensity reduced phytoplankton biomass, especially in the clear C treatment, and second, cyanobacteria constituted an important food source for microzooplankton grazers. This study adds to the increasing evidence that light effects can be counterintuitive and that cyanobacteria are not necessarily grazing resistant and can meditate nutrient transport to higher trophic levels.

This thesis characterizes the cycling of carbon, nitrogen and silicon by marine polar diatoms through the aid of a field study and a laboratory study. Field studies were conducted along a transect from Victoria, Canada to Barrow, Alaska and particulate carbon, nitrogen and silicon, chlorophyll a, nitrate, phosphate, silicic acid, and carbon and nitrogen incorporation, along with biogenic silica net incorporation were measured. Total primary production was lowest in the NE Pacific (0.3 to 1.0 mmol m-3 day-1), with new production contributing 17 to 38% of total production. Biogenic silica net incorporation in the upper 250 m of the water column in the NE Pacific was relatively low (0 to 0.12 mmol m-3 day-1), but positive, indicating the opportunity for export from the euphotic zone. Total primary, new production and production by siliceous plankton was highest in the Chukchi Sea, due to the influence of nutrient influx from the Anadyr Stream. Total primary production ranged from 1.0 to 3.2 mmol m-3 day-1, new production contributed as much as 56% of total production, and the production by siliceous phytoplankton was as high as 5.6 mmol m-3 day-1. Siliceous biomass was usually recycled in the upper water column of the Bering and the Chukchi Seas, in contrast to the NE Pacific. The interference of lithogenic material on the measurement of biogenic silica was explored using data from the Bering and Chukchi Seas. Results show that lithogenic interference is location specific. Sediment clay composition data should be considered when high concentrations of lithogenic silica are present. The laboratory study examined the effects of different irradiance and temperature conditions on two polar diatom species: Thalassiosira antarctica and Porosira glacialis. Temperature and irradiance had species-specific effects on the cellular content of carbon, nitrogen and silicon. The relationship between growth rate and silicon content for T. antarctica showed that silicon content increased as growth rate decreased, which is in agreement with previous studies. However, this relationship did not hold for P. glacialis at low temperatures. These species-specific effects complicate the understanding of how environmental change will influence phytoplankton populations in Arctic and sub-Arctic marine areas. In general, primary production was lower in the Bering and Chukchi Seas when compared to previous studies, however it is unknown whether differences are due to interannual variability or a trend of decreasing production. Data from both the field and laboratory component indicate a high amount of biological silicon cycling in polar environments. This study represents the first time net silicon incorporation has been measured as far north as the Chukchi Sea.