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Thesis (Ph. D.)--University of Adelaide, Dept. of Horticulture, viticulture and ocnology, 1996. / Copy of author's previously published article inserted. Includes bibliographical references (leaves 127-154).

Pistacia is an economically important genus because it contains the pistachio crop, P. vera, which has edible seeds of considerable commercial importance. The evolutionary history of the genus and the taxonomic relationships among the species are controversial and not well understood. This study that has been conducted on this genus to refine taxonomic and evolutionary relationship utilizing different types of data (including morphology, anatomy and molecular) The studied species were the following: Pistacia aethiopica J. O. Kokwaro, P. atlantica Desf., P. chinensis Bunge, P. eurycarpa Yaltirik, P. falcata Becc. ex Martelli, P. integerrima Stew. ex Brand., P. khinjuk Stocks, P. lentiscus L., P. mexicana HBK, P. mutica Fisch. & Mey., P. palaestina Boiss., P. terebinthus L., P. texana Swingle, P. vera L., and P. weinmannifolia Poiss. ex Franch. Phylogenetic analysis based on morphological data strongly supported the monophyly of Pistacia. The genus divided into two monophyletic groups. One group (Section Pistacia) contains P. atlantica, P. chinensis, P. eurycarpa, P. falcata, P. integerrima, P. khinjuk, P. mutica, P. palaestina, P. terebinthus, and P. vera while the other group (Section Lentiscus) contains P. aethiopica, P. lentiscus, P. mexicana, P. texana, and P. weinmannifolia. In anatomical analysis, all species had anomocytic stomata. In most species, the stomata density was higher on the abaxial surface than the adaxial. The ratio of abaxial to adaxial stomatal density varied from 0.0 to 1.7. Stomatal distribution may provide insights into how Pistacia species evolve in terms of leaf anatomy and respond to different climatic changes. Stomatal distribution changed (losing stomata on either surface) as the genus moved into regions of higher rainfall. This study revealed leaflets of P. vera, which have random orientation, were isobilateral, while leaflets of the other species were dorsiventral and were oriented horizontally. RAPD analysis showed that P. khinjuk and P. vera are very close species. This study provides more insights into understanding the evolution, taxonomy and genetics of this economically important genus. / Ph. D.

Copy of author's previously published article inserted. Bibliography: leaves 127-154. Pollination and fruit development were investigated in relation to abscission and abnormalities, specifically, blanking, semi-blanking, non-splitting and premature splitting of fruit. Pollen germination was assessed in Pistacia vera, P. atlantica and P. terebinthus. The pollen tube pathway in pistachio was documented precisely. Growth periods of normal and abnormal pistachio fruits were investigated.

Copy of author's previously published article inserted. / Bibliography: leaves 127-154. / xiv, 155 leaves, [19] leaves of plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Pollination and fruit development were investigated in relation to abscission and abnormalities, specifically, blanking, semi-blanking, non-splitting and premature splitting of fruit. Pollen germination was assessed in Pistacia vera, P. atlantica and P. terebinthus. The pollen tube pathway in pistachio was documented precisely. Growth periods of normal and abnormal pistachio fruits were investigated. / Thesis (Ph.D.)--University of Adelaide, Dept. of Horticulture, Viticulture and Ocnology, Waite Agricultural Research Institute, 1996

Cette thèse étudie la dynamique de la végétation Holocène pour Burmarrad dans le NW Malte et fournit une reconstruction paléoclimatique quantitative à base de pollen pour cet archipel méditerranéen situé au centre. Le record de pollen donne un nouvel aperçu l'échange végétation 7280-1730 cal BP qui correspondent bien avec other régional dossiers. La reconstruction du climat fournit également forte corrélation avec les sites du sud (en dessous de 40oN) de la Méditerranée. L'interprétation suggère un paysage initialement ouvert au début du néolithique, se développer en un dense Pistacia brousse ca. 6700 calBP. Depuis environ 4450 calBP le paysage devient de nouveau ouvert, coïncidant avec le début de l'âge du bronze sur l'archipel. Cette période coïncide avec une instabilité accrue du climat (entre 4500 et 3700 calBP) qui est suivie par une diminution progressive de la disponibilité de l'humidité de l'été à la fin de l'Holocène. Durant la période romaine début de l'occupation (1972-1730 calBP) le paysage reste généralement ouvert avec une augmentation modérée de Olea. Cette augmentation correspond à des preuves archéologiques pour la production de l'huile d'olive dans la région, avec l'augmentation des taxons cultivés des cultures et des espèces rudérales associés, ainsi que d'une hausse des cas d'incendies. Cette thèse propose également une synthèse des résultats d'un autre noyau (BM1) provenant de la même zone de chalandise, ainsi que les résultats d'une étude préliminaire de la pluie de pollen de surface moderne. L'archipel fournit des indications sur la végétation, les impacts humains et les changements climatiques dans un contexte de l'île au cours de l'Holocène. / This thesis investigates the Holocene vegetation dynamics for Burmarrad in north-west Malta and provides a pollen-based quantitative palaeoclimatic reconstruction for this centrally located Mediterranean archipelago. The pollen record from this site provides new insight into the vegetation changes from 7280 to 1730 cal BP which correspond well with other regional records. The climate reconstruction for the area also provides strong correlation with southern (below 40oN) Mediterranean sites. The interpretation suggests an initially open landscape during the early Neolithic, surrounding a large palaeobay, developing into a dense Pistacia scrubland ca. 6700 cal BP. From about 4450 cal BP the landscape once again becomes open, coinciding with the start of the Bronze Age on the archipelago. This period is concurrent with increased climatic instability (between 4500 and 3700 cal BP) which is followed by a gradual decrease in summer moisture availability in the late Holocene. During the early Roman occupation period (1972 to 1730 cal BP) the landscape remains generally open with a moderate increase in Olea. This increase corresponds to archaeological evidence for olive oil production in the area, along with increases in cultivated crop taxa and associated ruderal species, as well as a rise in fire events. This thesis also provides a synthesis with the results from another core (BM1) taken from the same catchment area, as well as results of a preliminary modern surface pollen rain study. The Maltese archipelago provides important insight into vegetation, human impacts and climatic changes in an island context during the Holocene.

L’extraction par fluide supercritique en particulier à l’aide du dioxyde carbone, présente plusieurs avantages par rapport aux procédés d’extraction classiques tels que le gain du temps, la sélectivité et l'absence de dégradation des substances extraites. Dans ce contexte, l’extraction des fleurs de Myrte et de Pisticia a été étudiée en déterminant leurs conditions optimales pour avoir un bon rendement. Cette étude a montré pour les deux plantes un rendement élevé en travaillant à des pressions importantes à l'ordre de 200 bars, à une basse température de 40°C et pour des particules de petites tailles de l’ordre de 220 µm. L'extrait de Myrte a montré une activité antioxydante meilleure que celle de l’huile essentielle de la même plante avec un IC50 de l‘ordre de 30 µg/ml. L'optimisation du pouvoir antioxydant des extraits de Pistacia par CO2 supercritique a montré un comportement différent de celui du rendement et pour des faibles pressions d’extraction (80 bars), l'activité antioxydante est meilleure. A 84 bars, l'étude a montré la possibilité d'obtenir un extrait présentant un rapport qualité prix meilleur pour des installations supercritiques industrielle de grandes tailles. Afin de valoriser nos recherches et de les concrétiser, l’ajout des extraits et des huiles essentielles a des produits agroalimentaires a enrichi et a donné une valeur ajoutée qui peut représenter ure innovation dans le domaine agroalimentaire. Finalement, les résultats expérimentaux de l’extraction par CO2 supercritique d'extrait de Pistacia, ont été modélisés par le biais du modèle de Sovova (1994) et les résultats numériques de simulation obtenus sont en accord avec les travaux expérimentaux réalisés. / The supercritical fluid extraction, using carbon dioxide, has several advantages over conventional methods of extraction such as the gain of time, selectivity and absence of extracted substances degradation. In this context, the extraction of myrtle flowers and Pistacia were studied by determining their optimal conditions for a good performance. This study showed for both plants high efficiency when working at high pressures in the order of 200 bars, at a low temperature of 40 ° C and for small particle sizes of the order of 220μm. The myrtle extract showed a better antioxidant activity than the essential oil of the same plant with an IC50 of about 30 μg / ml. The optimization of antioxidant extracts from Pistacia supercritical using CO2 showed different results from those for performance and we have good antioxidant activity for pressures of about 80 bars. At 84 bars, the study showed that we can have an extract with a better price-quality ratio for industrial supercritical installations of large sizes. The addition of extracts and essential oils to food products was enriching and has given an added value which can represent an innovation in the food industry. Finally, the experimental results for the extraction with supercritical CO2 of Pistacia extract were modeled through Sovova model (1994) for its reliability and the obtained numerical results agree with previous studies in the literature.

H. pylori is one of the most significant discoveries in gastroenterology in the past century. It is associated with a wide range of gastroduodenal pathologies and gastric cancer. Antibiotic resistance in H. pylori has emerged as a significant clinical problem. The body of work contained within this dissertation was carried out to investigate an alternative therapy based on observations of the traditional therapy for gastric disease in the Middle East. One of these traditional therapies centres on plants belonging to the Pistacia genus. This study represents the first reported investigation into the composition and biological activity of the trunk bark exudates of Pistacia atlantica Kurdic (P. a. Kurdica), Pistacia atlantica Mutica (P. a. Mutica) and Pistacia atlantica Cabolica (P. a. Cabolica), resinous gums that have been termed here ‘Kurdica Gum’, ‘Mutica Gum ’ and ‘Cabolica Gum ’ respectively. The antimicrobial screening of the trunk exudates of the genus Pistacia led to the characterization of the most active fraction of the Kurdica gum. This fraction was subsequently subjected to sub-fractionation leading to the discovery of fundamentally new information that went beyond H. pylori, expanding the original parameters of the project. The extent of these findings suggests that new classes of antibiotics might have been discovered. Primary studies on their structure and potential mechanism of action has been undertaken. Thirteen novel antimicrobial agents were identified. Based on the characteristics of these isolated fractions, 50 new compounds were modelled; of which 30 hypothetically have an MIC consistent with contemporary antibiotics and could represent viable lead compounds for commercial development. / Doctor of Philosophy

Resin found within Canaanite amphorae from the Late Bronze Age shipwreck discovered off the coast of southwest Turkey at Uluburun has previously been identified as Pistacia sp. Although evidence from Egypt suggests that this resin was in high demand and typically transported in such amphorae, it has also been proposed that the amphorae contained wine, with the resin used to seal the interior surfaces and to flavour and/or preserve the wine. To attempt to resolve this question, we have analysed five samples of pistacia resin found in amphorae from the shipwreck using a range of analytical techniques which have used in the past for the analysis of wine residues: spot tests, FT-IR, and HPLC-MS-MS. As well as the archaeological samples, we have analysed modern samples of pistacia resin, leaves and fruit to determine the effectiveness of each technique and to exclude the possibility of false positive results. In addition to the analyses for wine we also detail analysis (GC-MS) of the terpenoids for the purpose of further molecular characterisation of the resin. Bulk stable isotope analysis was used in comparison with similar resins to attempt to identify the geographical origin of the resin.

Thesis (Ph.D.) -- University of Western Sydney, 2006. / A thesis presented to the University of Western Sydney, College of Health and Science, School of Biomedical and Health Sciences, in fulfilment of the requirements for the degree of Doctor of Philosophy. Includes bibliography.