The work concerns in modern surface treatment, such as cold plasma. The main aim of this work was to find out the effect of plasma treatment on the surface of wood applied before glue treatment which is influential in final quality of glueing. The work compares the firmness of glued joint of solid wood-fibre slab -- HDF and locust tree on groups of samples of treated and non-treated surface in different plasma discharges. The first part of the work comprises a summary of knowledge in the plasma field and glueing and it attempts to establish a general overview of present development in research in the field of examined issue. The second part of the work deals with method of performed experiment according to the researched norms. Results of the work have proven that plasma preparatory treatment influences the firmness of glueing and at a certain type of use of plasma discharge we can improve the quality of glued joint.

The thesis deals with the influence of cold plasma,as a stress factor, on content and fluorescence of chlorrophylls of oilseed rape. Theoretical part deals with characteristic oilseeds rape, fluorescence, photosynthetic pigments and the effects of cold plasma on on physiology of plants. The theoretical part continues in experimental mesurement. The eperiment was done in laboratory conditions. The own of eperiment was to discover the influence of cold plasma on the amount of chlorophylls and fluorescence of chlorophylls. The rape seads were treated with cold plasma for the duration of various time interval. The thesis considersif the plants, which were sprouted from treated seeds, show higher values of physiological activities as compared with plants germinated from conventional seed.

Plasma protein shave many important functions in the organism. Gamma globulins are carriers of immunoglobulins which play animportant role in the immune response. Their contentis primarily given by the health burden of the organism. The aimofthe study was to determine the individual protein fractions in the blood plasma of ewes and lambs, comparemutual relations between total plasma proteins and thein variol factions and assess the concentrations of individual plasma proteins, mainly globulin, in connection with the aktivity of the thyroidgland, physiological state, and increments. The sampling was carried out in the spring (25.3.) and the autumn (14.10) 2013. The individual protein fractions were determined from the serum by the means of electrophoresis. The results show that the concentration of proteins in the blood plasma of bothewes and ewe lambs and ram lambs was high due to haemoconcentration, heat stress during sampling, grazing young green forage, comprising a large number of protein aceous substances, and increasing demands on energy for milk production, especially in the early stagesoflactation. Theconcentrationofproteins in theblood plasma oflambspertains to thegrowthproduction. It establishes a correlation between the concentration of plasma proteins and the aktivity of the thyroidgland. It also confirmes higher concentrations of plasma proteins of lambs correlating to thein higher daily gain. Due to the high concentration of total protein in the plasma, the level of its fractions was high as well. After the conversion to a percentage, the level of - globulins in blood plasma in all categories was within normal limits in the range of 14-27%. The concentration of - globulins in blood plasma increased in relation to the parasitological findings coccidia of the genus Eimeria and gastrointestinal nematodes. Relations between the kontent of plasma proteins in the blood of the lambs and ewe sobserved were in most casespositive. There was a strong dependence between the total protein and globulins. Even among other fractions of plasma proteins and globulins correlation coefficient was almou always positive.

This diplom theses is concerned with the possibilities of using non-thermal plasma in biomedical and enviromentally friendly applications. The theoretical part is drawn up as research overview of the current knowledge from the stated issue. The practical part is focused on the modification of nanofibres by deposition of TiOx layers.

The thesis deals with methods for viability evaluation of the phytopathogenic bacterium Xanthomonas euvesicatoria after low-temperature plasma treatment. Low-temperature plasma produced by Gliding Arc experimental device was used for treatment of X. euvesicatoria. The viability of the bacterial cells was assessed using a scanning electron microscope (SEM) and by measuring of the fluorescence in the Smart-DART device using PrestoBlue chemical reagent. Methodology has been optimised for the sample preparation for the treatment by low temperature plasma and used for evaluation of applied methods. Lethal effect of gliding arc plasma to this gram-negative bacteria was verified by SEM which showed. There is significant structural changes on the cell surface. Viability assessment of X. euvesicatoria using Smart-DART device is a fast, time-saving and inexpensive evaluation of cell viability. The great advantage of this device is its ability to measure the fluorescence in real time. The disadvantage of this method is lower reliability in current stage of research.

The thesis is focused on increasing the surface tension of polyethylen, using plasma. A gliding arc was chosen for this surface modi cation. The theoretical part includes an overview of gas discharges, their classi cation, properties and technical applications, and deals with an in uence of surface tension on surface modi cations of materials. The experiment is explained in the practical part, a jet analysis carried out before the experiment is described. Results of the experiment are presented.

This thesis contains a brief summary of the theoretical applications of plasma discharges, and a description of the principle Gliding Arc plasma source. The practical part is focused on the research of the influence ofcereal seed treatment using plasma discharge on their nutritional value (dry matter, ash, protein and fiber). In the conclusion of this thesis is evaluation of the whole experiment.

This diploma thesis is aimed to the possibilities of using low-temperature plasma in various fields of science, research and production with the main focus is given to applications in medicine and environmental applications. In the practical part is presented design a computer model for simulation of the plasma behavior in the above areas. This model is a hybrid type and as a working gas is used oxygen.

Primordial gonocytes (PGCs) in some animals, including fish arise after fertilization in extragonadal region from maternally inherited germline cytoplasm, and migrate to the future gonads region during embryogenesis, where differentiate into gametes. PGCs formation and migration patterns have been studied in several species models, and it is known that these patterns differ from each other. Sturgeons belong to class ray-finned fishes(Actinoptergii), in which the sturgeon phylogenetic position is an out-group to teleost fishes, the sturgeon development pattern is more similar to amphibians than teleost fishes. In this study, we demonstrate an injection technique for sturgeon PGCs visualization by GFP nos1 3'UTR mRNA. We found that the Sterlet(A. ruthenus) PGCs are specified in the vegetative pole of the embryo. Subsequently, we reported the PGCs migration route. The arisen PGCs actively migrated on the yolky cell mass, yolky extension, and after that passively moved to gonadal ridge. This study provides evidence that the PGCsare specified by maternally inherited germplasm, located in the vegetative part of the embryo. Sturgeon PGCs specification was similar to that of anuras, but the migration pattern resembled that of teleost. Furthermore, we successfully isolated PGCs to next needed studies.

The objective of this study was to evaluate the effect of intrauterine plasma infusion on conceiving rate in selected group of mares during the breeding seasons 2008 and 2009. The fresh sperm insemination was followed by the infusion of self-plasma and subsequently the successivity of the insemination was compared with the control group of mares. Next, there were observed following factors which could influence the effectiveness of conceiving: age of the mares, number of insemination doses, size and location of preovulating follicle, month of the year. There was also evaluated the difference between multiparous (with one and more births) and nulliparous mares. The statistical analyses were proceeded by the use of contingency tables and the one-factorial analysis of variance (ANOVA). Next, the regression and correlation analyses were used. All the statistical procedures were performed with the use of statistical software STATGRAPHICS Plus 5.0. The results showed that there were the same results in observing of both number of insemination doses and fertile heats evaluation. There was found no significant effect of plasma infusion on the conceiving rate. In the model with the effects of plasma infusion and the age of the mare there were found statistically significant differences. It means that the effect of plasma infusion on conceiving rate was confirmed in the mares of the age up to 14 years. The result of the following analysis is the non-significant difference in conceiving rate between the groups of multiparous mares with or without plasma infusion.