abstract: This research addresses human adaptive decisions made at the Pleistocene-Holocene transition - the transition from the Last Glacial Maximum (LGM) to the climate regime in which humankind now lives - in the Mediterranean region of southeast Spain. Although on a geological time scale the Pleistocene-Holocene transition is the latest in a series of widespread environmental transformations due to glacial-interglacial cycles, it is the only one for which we have a record of the response by modern humans. Mediterranean Spain lay outside the refugium areas of late Pleistocene Europe, in which advancing ice sheets limited the land available for subsistence and caused relative demographic packing of hunter-gatherers. Therefore, the archaeological records of Mediterranean Spain contain more generally applicable states of the Pleistocene-Holocene transition, making it a natural laboratory for research on human adaptation to an environmental transformation. Foragers in Mediterranean Spain appear to have primarily adapted to macroclimatic change by extending their social networks to access new subsistence resources and by changing the mix of traditional relationships. Comparing faunal records from two cave sites near the Mediterranean coast with Geographic Information System (GIS) reconstructions of the coastal littoral plain from the LGM to the Holocene indicates the loss of the large ungulate species (mainly Bos primigenius and Equus) at one site coincided with the associated littoral disappearing due to sea level rise in the late Upper Paleolithic. Farther north, where portions of the associated littoral remained due to a larger initial mass and a more favorable topography, the species represented in the faunal record were constant through time. Social boundary defense definitions of territory require arranging social relationships in order to access even this lightly populated new hunting area on the interior plain. That the values of the least-cost-paths fit the parameters of two models equating varying degrees of social alliance with direct travel distances also helps support the hypothesis that foragers in Mediterranean Spain adapted to the consequences of macroclimatic change by extending their social networks to gain access to new subsistence resources Keeping these relationships stable and reliable was a mitigating factor in the mobility patterns of foragers during this period from direct travel to more distant down-the-line exchange. Information about changing conditions and new circumstances flowed along these same networks of social relationships. The consequences of climate-induced environmental changes are already a concern in the world, and human decisions in regard to future conditions are built upon past precedents. As the response to environmental risk centers on increasing the resilience of vulnerable smallholders, archaeology has an opportunity to apply its long-term perspective in the search for answers / Dissertation/Thesis / Ph.D. Anthropology 2013

This doctoral thesis presents the results of the pioneering archaeological investigation conducted in the Northern Mexican Highlands with the aim to evaluate the existing indicators of the earliest human occupations at the end of the Pleistocene and discover new evidence of ancient cultural manifestations through a systematic exploration of an endorheic basic in the Zacatecas desert, a region never studied before. An exhaustive survey and analysis of the available literature on Mexican prehistory establishes the weak points of the local paradigms, differentiating between academic myths and objective realities. A complete historiography of the topic of the earliest humans in Mexico has been achieved, for the first time. The study of several collections of flaked stone artefacts, in different cities in Mexico, show new indicators of the presence of bearers of the Late Paleoamerican cultures, in regions where their presence had been weakly confirmed. The most important part of the research consisted in fieldwork realised during two long seasons; the first one dedicated to the surface explorations and the second one to excavations. Thirty-five new archaeological sites were discovered in the first phase, most of them open campsites reminiscent of hunter-gatherer societies, with a richness of stone artefacts on their surface. They indicate a long cultural sequence, going from the Late Pleistocene to the Late Holocene and the historic periods. Four sites were further studied by fourteen test excavation units: Dunas de Milpa Grande, San José de las Grutas, the Chiquihuite Cave and Ojo de Agua. Two new archaeological cultures were identified, one at Dunas (an interesting assemblage of limestone and basalt flaked stone tools) and another one at San José (a limestone concave-based points complex). First indicators of ʻolder than Clovisʼ human presence have also been obtained. The palaeoenvironmental data provide a preliminary reconstruction of the Late Pleistocene-Early Holocene landscape of the basin, based on geology, extinct fauna, phytolith and mollusc analyses. Radiocarbon and OSL results support a first cultural and paleoclimatic model for the study area. This investigation also discovered the first case of a “black mat” in Mexico: a black layer of sediment deposited under specific environmental conditions during the Younger Dryas cooling event.

This dissertation describes the results of archaeological survey and excavationsundertaken on the Mesa el Chaparral in the county of Mina in Nuevo Leon, Mexicoduring 2001. Sixty-six previously undocumented archaeological sites were discovered onthe arid surface. Excavations found no intact subsurface deposits, but a wealth of surfacedata was collected. Subsequent analyses demonstrated a forager lifeway for the majorityof the Holocene human occupation of the region in a remarkably stable pattern.To understand sites found on the deflated modern surface necessitatedcontemplation of the basic theories and models used in hunter-gatherer research. Thisallowed for the construction of new diagrams designed to hypothesize fundamentalrelationships between general aspects of the lifeway including environmental factors, sitesize and visibility issues, and human mobility patterns. From some basic continuums,more detailed diagrams were created that allow understanding and prediction of humanbehavior based upon data found from artifacts and features. After testing their salience,the models were dynamically combined with the site data and ethnographic analogies toarrive at an understanding of the human lifeways represented by the recoveredarchaeological data. This provided a fascinating look into the day-to-day lives of thegeneralized mobile foragers of prehistoric northeastern Mexico.Included in the recovered data are hearth features, lithic debitage and artifacts,and basic site descriptions. Archaeological locations ranged from small with a singlefeature to over a square kilometer with over 100 features, all located on the surface wherethey are subject to wind deflation and water erosion. Most of the sites containeddiagnostic artifacts from the entire Holocene, further compounding the analyticcomplexity of the project. Understanding the context of the data and making use of themodels and ethnographic analogies, it was estimated that every site represented anoccupation by a small band of mobile forgers making generalized use of the resourcesavailable in the region. Making residential moves often allowed people to survive in theharsh environment. Few lifeway changes were noted prior to Spanish influence in theregion from the time the environment became arid at the end of the Pleistocene.

This article presents key data and discussion about the initial peopling of northwestern South America, highlighting its strategic significance in the continental context, cultural variability and landscape change over time. In Colombia there is very little relevant information regarding occupations prior to 12,000 BP; in contrast, the information is abundant relative to a number ofearly contexts during the Pleistocene-Holocene transition. The variability among unifacial and bifacial lithic assemblages reported in different physiographic regions indicates alternative models on early settlement to regional level. Considering the importanceof the inter-Andean valley of the Magdalena River, this article emphasizes the archaeological contexts and paleoenvironmental information, highlighting environmental impacts associated not only to global climate change, but in particular of the effects ofvolcanism. The article stresses the findings and chronologies of bifacial lithic assemblages of the Magdalena Valley in relation to the early archaeology of northwestern South America. / En este artículo se presentan los principales datos y reflexiones asociados al poblamiento inicial del noroccidente de Sudamérica debido a su significado estratégico en el marco continental, se destacará la variabilidad cultural y se enfatizarán los cambios depaisajes en el transcurso del tiempo. En Colombia existe muy poca información relevante relacionada con las ocupaciones anterioresa 12.000 AP; en contraste, la información es abundante en cuanto al número de contextos tempranos durante la transición Pleistoceno-Holoceno. La variabilidad entre los conjuntos líticos unifaciales y bifaciales reportados en distintas regiones fisiográficas plantea propuestas de modelos alternativos sobre el poblamiento temprano a escala regional. Considerando la importancia del valle interandino del río Magdalena, se recalcan los contextos arqueológicos e información paleoambiental. Asimismo, se destacan los impactos ambientales que demuestran por qué no perduraron las evidencias del Pleniglacial, lo que podría deberse a efectos asociados no solo al cambio climático global, sino, en particular, a causa del vulcanismo. Este trabajo resalta los hallazgos y cronologías de los conjuntos líticos bifaciales del valle del Magdalena en relación con la arqueología temprana del noroccidente de Sudamérica.

Après une simplification significative des méthodes de débitage durant l'Azilien, rompant progressivement avec les normes magdaléniennes, le retour au cours du Dryas récent à des productions lamino-lamellaires élaborées marque un tournant abrupt dans la tradition lithique. Bien qu'un certain flou pèse sur la chronologie des événements, les recherches menées depuis quelques années autour des industries de la transition Pléistocène-Holocène permettent de bien cerner les transformations survenues dans les modalités et les objectifs des productions lithiques. En revanche, malgré le fait que les interrogations des chercheurs soient largement orientées vers des reconstitutions d'ordre palethnographiques, les finalités fonctionnelles des outils, leur modalité d'emploi et les chaînes opératoires dans lesquelles ils sont impliqués restent des thèmes de recherche très peu abordés. C'est afin de pallier ce manque que nous avons exploré ces questions à travers l'analyse tracéologique des industries lithiques de deux sites du nord-ouest de la France issus d'opérations récentes (Le Buhot à Calleville, Eure ; La Fosse à Villiers-Charlemagne, Mayenne). L'apport de ce travail est appréciable à différentes échelles spatio-temporelles. Sur le temps court, les résultats obtenus révèlent des contrastes saisissants entre les occupations, tant au regard des activités menées par l'intermédiaire des outils que de l'économie des produits des débitages, et enrichissent le modèle de complémentarité des sites déjà proposé. Sur le temps long, le croisement des données fonctionnelles acquises ces trente dernières années entre l'Azilien et le premier Mésolithique et les autres données du registre archéologique permet de discuter des inflexions dans les systèmes techniques et les économies préhistoriques. / After a significant simplification of the flint reduction methods during the Azilian period, which broke gradually with Magdalenian standards, the return to sophisticated laminar productions during younger Dryas marks a strong split in the lithic tradition. Beyond a state of uncertainty towards chronological boundaries, the research which have been conducted for thirty years gives a good understanding about changes in flint production methods and aims. Even though most research focuses on palethnographic reconstitutions, the functional purposes of the flint industry and the chaînes opératoires in which flint tools are implicated remain insufficiently studied. And yet, these questions are of prime importance to meet the expectation of the palethnographic reconstitutions that archaeologists covet. To overcome this deficiency, the functional analysis of two north-western France sites (le Buhot site at Calleville, Eure ; la Fosse site at Villiers-Charlemagne, Mayenne) attributed to the Pleistocene-Holocene transition were undertaken. The contribution of this doctoral research can be appreciated at different spatio-temporal scales. In the short-term, the results raise striking contrasts between sites, as much in regard to the activities performed as to the debitage products economy, and improve the current model of settlement patterns. In the long-term, the interplay of techno-functional results and other archaeological data gives food for thought about changes in the technical systems and prehistoric economies.

El objetivo de esta tesis doctoral es el análisis de las preferencias en la selección de los lugares de asentamiento por parte de las sociedades de cazadores-recolectores de la transición Pleistoceno Final - Holoceno (es decir, Magdaleniense y Aziliense) de la mitad oriental de la Cornisa Cantábrica. Más concretamente, se trata de de analizar, calculando una serie de características y variables, la localización de una serie de yacimientos asignados a este periodo, para tratar de identificar un patrón o patrones concretos en el tipo de lugares elegidos por las comunidades humanas para situar sus lugares de hábitat, si pueden diferenciarse distintos tipos de asentamientos en función de su localización, y si existe un cambio en las preferencias en la selección de los lugares de ocupación con respecto a momentos anteriores. Para llevar a cabo estos análisis, se emplea un Sistema de Información Geográfica. / The objective of this thesis is the analysis of preferences in the choice of settlement sites by the hunter-gatherers of Final Pleistocene - Holocene transition (i.e., Magdalenian and Azilian) from eastern Cantabrian coast. More specifically, the location of a number of sites is analyzed by calculating different characteristics and variables, to try to identify a specific pattern or patterns in the type of sites selected by human communities to bring their places of habitat, to differentiate different types of settlements based on their location, and whether a change in preferences in the selection of places of employment with respect to earlier periods. To perform these tests, a Geographic Information System is used to calculate the variables considered.

The archaeological literature warns against trusting the context of artifacts found within a vertisol due to the constant mixing of sediments caused by the shrink/swell properties of clays. These churning processes were thought to be the defining characteristic of vertisols until only the past few decades. It is now apparent that vertisols vary drastically based on a wide spectrum of variables and are fully capable of forming without churning processes. The Buttermilk Creek Site, Block A represents a prime example of a minimally developed vertisol. In addition, the site itself is a heavily occupied lithic quarry that has been almost continuously inhabited since Clovis and possibly Pre-Clovis times. This thesis takes a detailed look at the sediments and distribution of lithic artifacts from Block A of the Buttermilk Creek site to address the two following research objectives: 1) to determine if the archaeological context within the floodplain sediments at Block A has been disturbed by post-depositional processes, and 2) to identify discrete occupation surfaces within the vertic floodplain sediments at the site. These objectives are addressed using a variety of methods, including: 1) plotting the stratigraphic position of diagnostic artifacts, 2) determining the size distribution of debitage and artifact quantities throughout the floodplain deposits, 3) examining the distribution of cultural versus non-cultural lithic material, 4) recording the presence or absence of heat alteration in the deposits, 5) creating maps showing the degree of fissuring across the site, 6) analyzing differences in patination on artifacts, and 7) analyzing the presence of calcium carbonate on artifacts from all levels. Results from these analyses show that, despite the classification of sediments at Block A as a vertisol, vertical displacement of artifacts is largely absent. Chronologically ordered diagnostic points, consistently size sorted artifacts, and a lack of constant mixing of calcium carbonate throughout the profile suggest that artifacts found as deep as 20 cm below the Clovis-aged horizon represent intact cultural horizons. These oldest components found in Block A may represent some of the earliest known evidence of people in the New World.

Humans evolved in a world of giant creatures. Current evidence suggests that most ice age megafauna went extinct around the transition to our current Holocene epoch. The ecological reverberations associated with the loss of over 65% of Earth’s largest terrestrial animals transformed ecosystems and human lifeways forever thereafter. However, there is still substantial debate as to the cause of this mass extinction. Evidence variously supports climate change and anthropogenic factors as primary drivers in the restructuring of the terrestrial biosphere. Much of the ongoing debate is driven by the insufficient resolution accessible via macro-remains. To help fill in the gaps in our understandings of the Pleistocene-Holocene transition, I utilized the growing power of sedimentary ancient DNA (sedaDNA) to reconstruct shifting signals of plants and animals in central Yukon. To date, sedaDNA has typically been analyzed by amplifying small, taxonomically informative regions. However, this approach is not ideally suited to the degraded characteristics of sedaDNA and ignores most of the potential data. Means of isolating sedaDNA have also suffered from the use of overly aggressive purification techniques resulting in substantial loss. To address these limitations, I first experimentally developed a novel means of releasing and isolating sedaDNA. Secondly, I developed a novel environmental bait-set designed to simultaneously capture DNA informative of macro-scale ecosystems. When combined, we identify a substantial improvement in the quantity and breadth of biomolecules recovered. These optimizations facilitated the unexpected discovery of horse and mammoth surviving thousands of years after their supposed extirpation. I followed up these results by extracting DNA from multiple permafrost cores where we confirm the late survival signal and identify a far more complex and high-resolution dataset beyond those identifiable by complementary methods. I was also able to reconstruct mitochondrial genomes from multiple megafauna simultaneously solely from sediment, demonstrating the information potential of sedaDNA. / Dissertation / Doctor of Philosophy (PhD) / A new addition to the rapidly growing field of palaeogenetics is environmental DNA (eDNA) with its immense wealth of biomolecules preserved over millennia outside of biological tissues. Organisms are constantly shedding cells, and while most of this DNA is metabolized or otherwise degraded, some small fraction is preserved through sedimentary mineral-binding. I experimentally developed new ancient eDNA methods for recovery, isolation, and analysis to maximize our access to these biomolecules and demonstrate that this novel approach outperforms alternative protocols. Thereafter, I used these methods to extract DNA from ice age permafrost samples dating between 30,000–6,000 years before present. These data demonstrate the power of ancient eDNA for reconstructing ecosystem change through time, as well as identifying evidence for the Holocene survival of caballine horse and woolly mammoth in continental North America. This late persistence of Pleistocene fauna has implications for understanding the human ecological and climatological factors involved in the Late Pleistocene mass extinction event. This effort is paralleled with megafaunal mitogenomic assembly and phylogenetics solely from sediment. This thesis demonstrates that environmental DNA can significantly augment macro-scale buried records in palaeoecology.