Global ETD Search

251	Comparison of two automated DNA amplification systems with culture for detection of Chlamydia trachomatis and Neisseria gonorrhoeae infections in symptomatic men Yau, Chong-yee, Miranda. January 2000 (has links) Thesis (M. Med. Sc.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 35-42).
252	Occurrence of tick-borne haemoparasites in nyala (Tragelaphus angasii) in KwaZulu-Natal and Eastern Cape Province, South Africa Pfitzer, Silke. January 2010 (has links) Thesis (MSc (Veterinary Tropical Diseases)--University of Pretoria, 2009. / Includes bibliographical references. Also available in print format.
253	Multiplex RT-PCR for typing and subtyping influenza and respiratory syncytial viruses / Lau, Wing-tong, Ricky. January 2002 (has links) Thesis (M. Med. Sc.)--University of Hong Kong, 2002. / Includes bibliographical references (leaves 42-47).
254	Control of influenza: detection and antivirals Jayawardena, Shanthi. January 2007 (has links) published_or_final_version / Microbiology / Doctoral / Doctor of Philosophy Avian influenza - Diagnosis. Reverse transcriptase. Antiviral agents.
255	Detection of human enteroviruses by reverse transcription-PCR in hospitalized children with respiratory disease in Hong Kong Chan, Kit-man, 陳潔雯 January 2010 (has links) published_or_final_version / Microbiology / Master / Master of Medical Sciences Enteroviruses - Analysis. Reverse transcriptase.
256	Multiplex reverse transcription-PCR for detection and identification of human parainfluenza viruses 1,2,3 and 4 infection in hospitalizedchildren with respiratory disease in Hong Kong Lam, Siu-yan, 林小欣 January 2007 (has links) published_or_final_version / Medical Sciences / Master / Master of Medical Sciences Reverse transcriptase Parainfluenza viruses.
257	Androgen receptors in the bonnethead shark, Sphyrna tiburo: CDNA cloning and tissue-specific expression in the male reproductive tract Tyminski, John P 01 June 2007 (has links) Androgens and the androgen receptor (AR) play important roles in virilization, spermatogenesis, and sexual behavior in vertebrates. An understanding of the distribution and levels of expression of the ARs on the cellular and tissue level demonstrates the pattern of responsiveness to the androgenic hormones in a given organism. In this study, a fragment of the AR gene was cloned and sequenced from the bonnethead shark, Sphyrna tiburo, an elasmobranch species with a well-defined annual reproductive cycle. Acquiring this gene sequence facilitated the construction of species-specific AR polymerase chain reaction (PCR) primers and species-specific AR mRNA probes that were used to screen reproductive tissues for evidence of AR gene expression using reverse transcription (RT)-PCR and in situ hybridization (ISH), respectively. The RT-PCR screens demonstrated AR gene expression in the testes, epididymides, seminal vesicles, and claspers of male sharks. The use of relative PCR revealed that these organs have variable levels of AR gene expression that significantly differ with the stage of the shark's seasonal reproductive cycle. ISH results localized the AR RNA in the interstitial cells, Sertoli cells, and developing sperm of the testes, and mature spermatozoa within the seminal vesicles and the epididymides. Immunocytochemical methods used to detect the AR protein using a rabbit polyclonal antibody, PG-21, produced comparable results in the shark testes but did not yield positive results in the seminal vesicles or the epididymides. However, the Leydig gland, whose secretions contribute to the seminal fluid, demonstrated consistent AR immunoreactivity. Results of ICC in male and female embryos of S. tiburo revealed AR protein in the developing kidney but not in the embryonic reproductive structures. By characterizing AR distribution in the reproductive tract of male S. tiburo, this study provides the basis for future research on the direct and indirect effects of androgenic hormones in this species. Androgen receptor Steroid hormone Polymerase chain reaction Immunocytochemistry mRNA probe American Studies Arts and Humanities
258	Comparison of two automated DNA amplification systems with culture fordetection of Chlamydia trachomatis and Neisseria gonorrhoeaeinfections in symptomatic men 邱莊儀, Yau, Chong-yee, Miranda. January 2000 (has links) published_or_final_version / Medical Sciences / Master / Master of Medical Sciences Gene amplification. Polymerase chain reaction. Chlamydia trachomatis - Diagnosis. Gonorrhea - Diagnosis.
259	Evaluation of a multiplex polymerase chain reaction assay for detection of silent fluoroquinolone-resistant determining mutations instreptococcus pneumoniae Cheung, Yin-mei., 張燕湄. January 2003 (has links) published_or_final_version / Medical Sciences / Master / Master of Medical Sciences Polymerase chain reaction - Evaluation. Drug resistance.
260	Multiplex RT-PCR for typing and subtyping influenza and respiratory syncytial viruses 劉永棠, Lau, Wing-tong, Ricky. January 2002 (has links) published_or_final_version / Medical Sciences / Master / Master of Medical Sciences Respiratory syncytial virus. Influenza viruses. Viral genetics. Polymerase chain reaction. Reverse transcriptase.

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