No description available.

Submitted by Johnny Rodrigues (johnnyrodrigues@ufcg.edu.br) on 2018-11-10T13:49:36Z No. of bitstreams: 1 CÍCERO AURÉLIO GRANGEIRO LIMA - DISSERTAÇÃO PPGECA 1992..pdf: 25126677 bytes, checksum: 344ec8cc3722c36124b4e9b1b88b50b3 (MD5) / Made available in DSpace on 2018-11-10T13:49:36Z (GMT). No. of bitstreams: 1 CÍCERO AURÉLIO GRANGEIRO LIMA - DISSERTAÇÃO PPGECA 1992..pdf: 25126677 bytes, checksum: 344ec8cc3722c36124b4e9b1b88b50b3 (MD5) Previous issue date: 1992-11-12 / Testes de infiltração com infiltrômetro duplo anel de nível constante foram efetuados em 32 pontos de uma malha retangular de uma parcela agrícola da Fazenda Experimental da EMEPA - Pb. Ajustamentos as Leis de Philip e de Green e Ampt foram efetuados. Medições da granulometria, densidade aparente seca do solo e umidade volumétrica antes e depois dos testes foram também efetuadas. Utilizou-se a seguir técnicas de análises estatística clássica e geoestatística para caracterizar a variabilidade espacial dos parâmetros de infiltração obtidos pelos ajustamentos. Nenhum dos parâmetros estudados apresentou estrutura espacial definida. Correlações simples e múltiplas evidenciaram dependência da percentagem de Argila + Silte com o espaço físico, e independência entro os parâmetros de infiltração. Ilustrações sobre o efeito da variabilidade espacial sobro o ponto de vista agronômico o hidrológico são mostradas. / Infiltration tests with double-ring infiltrometers were performed in a 33 paint reiangular regular]y spaced grid of an agri cultural site. Fitting of the infiltration curve obtained by Philip and Green and Ampt laws allowed to obtain the sorptivity, the infiltration rate at steady state and the suction parameters of Green and Ampt,for each point. Measurements of pre- and post-humidi ty, granulometry and dry density were also performed. Classical statistical and geo-statistical techniques were used to characterize spatial variability. None of the parameters studied showed a defined spatial structure. Single and muitiple correlations showed a dependence between Cclay + siltD percentage with space but no correlation between parameters was obtained. I11ustrative considerations about spatial variability were ma de with agronomic and hydrologic applications.

L’ingénierie du tissu osseux vise à concevoir un substitut tissulaire associant des cellules ostéoprogénitrices à une matrice tridimensionnelle capable de promouvoir la reconstruction osseuse, ouvrant la voie au développement de thérapeutiques substitutives à la pratique de la greffe dont les limitations sont bien connues.Le but de ce travail a été de développer un nouveau produit d’ingénierie tissulaire (PIT) destiné à la régénération osseuse constitué i) d’une matrice tridimensionnelle poreuse constituée de polysaccharides naturels biodégradables, ii) de cellules souches adultes issues du tissu adipeux humain (ADSCs) et d’identifier les conditions de culture optimales permettant le développement d’un produit fonctionnel pour une utilisation clinique. Nos résultats montrent que l’architecture et la composition de la matrice macroporeuse polysaccharidique permet de guider la différenciation ostéoblastique des ADSCs, en l’absence de facteurs ostéogéniques, et notamment en conditions de culture dynamique, grâce à l’organisation cellulaire en agrégats promouvant les interactions cellulaires. Les ADSCs peuvent être marquées à l’aide de nanoparticules superparamagnétiques et suivies in vivo de façon non invasive par imagerie par résonnance magnétique (IRM) au sein des matrices après leur implantation en site sous-cutané chez la souris. Les images IRM montrent que le matériau permet de délivrer une partie des cellules au niveau du site d’implantation participant probablement à un processus de réparation tissulaire. Enfin, en vue d’applications cliniques, un milieu de culture sans sérum répondant aux conditions GMP (Good Manufacturing Practices) pour la différenciation ostéoblastique a été développé par un industriel et validé au cours de ce travail de thèse.En conclusion de ces travaux, l’association d’une matrice macroporeuse composée de polysaccharides avec des ADSCs dans des conditions de culture spécifiques, en conditions dynamiques, semble pertinente et prometteuse pour des applications cliniques en ingénierie du tissu osseux. / Bone tissue engineering may associate osteoprogenitor cells to a tridimensional scaffold that can promote tissue reconstruction in order to replace bone grafting strategies whose limitations are well known. This study aims to develop a new tissue-engineered construct for bone regeneration constituted by i) a tridimensional polysaccharide-based scaffold, ii) adult stem cells extracted from human adipose tissue and identify the best culture conditions needed to develop a functional construct for clinical use. Our results show that this macroporous scaffold offers, without any osteoinductive factors, a suitable architecture and composition for driving osteoblastic differentiation of ADSCs especially when placing the tissue-engineered construct in dynamic conditions, thanks to cell aggregate conformation promoting cell-to-cell interactions. Thanks to ADSCs labeling, the tissue-engineered construct can be tracked in vivo in a non invasive way by magnetic resonance imaging (MRI), after their subcutaneous implantation. Results evidenced that this scaffold behaves as a cell carrier for of holding in its own cell fraction and delivering another fraction to the site of implantation for inducing a better tissue regeneration process. Finally, a serum free medium meeting standards GMPs (Good Manufacturing Practices) has been developed for inducing ADSCs osteoblastic differentiation as a first step towards clinical application.In conclusion, this polysaccharide-based scaffold associated with ADSCs, cultured under low fluid flow in a new bioreactor device, could be a relevant and promising tissue engineered construct for bone tissue engineering applications.