This research reported here was carried out to examine alternatives to antibiotic growth promoters as a result of their being banned in the animal feed industry. Four experiments were conducted to evaluate the efficacy of non-medicated feed additives as replacements for antibiotic growth promoters in broiler feeds. The additives used were enzymes (a new thermo-tolerant powder enzyme called TXAP, phytase, lipase and a new phytase enzyme derived from E. coli called phyzyme XP), organic acid (Acid Pak), prebiotic (Bio-Mos®) and probiotic (All-Lac XCL). Mashed maize-soya based feeds were used in all the experiments, which were conducted in litter-floor pens. The first experiment was a dose-response trial. Broilers in eight replicate pens of 50 males and 50 females were fed unsupplemented feeds and five additional feeds containing increasing levels of TXAP, from 0.5 to 2.5 g/kg to 42 d. The second experiment used enzyme TXAP with two different enzymes (phytase and lipase), individually or in combination. Six replicate pens of 50 males and 50 females were fed either unsupplemented feeds or one of six additional feeds treated with TXAP, lipase, phytase , a combination of TXAP and lipase, a combination of TXAP and phytase or a combination of all the three enzymes . This trial continued for 42 d. In the third experiment three types of TXAP (Lot 1, 2 and 3) were used, with fixed levels of xylanase and amylase but varying levels of protease activities (4000, 2000 and 1000 U/kg for Lot 1, 2 and 3, respectively) in combination with phyzyme XP for 35 d. The fourth experiment used mannan-oligosaccharide (Bio-Mos®), organic acid (Acid pak 2x), probiotic (All Lac XCL 5x), individually or in combination and an antibiotic growth promoter (Zinc bacitracin) for 42 d. The chickens in this experiment were challenged with Clostridium perfringens (CP) at 21, 22 and 23 d to determine the efficacy of these additives for replacing antibiotics in hindering the effects of CP on the villus surface area. The dose-response trial did not show any significant improvement in broiler performance with any level of inclusion of enzyme TXAP. The results from this study showed some beneficial effects with the use of enzyme TXAP when fed alone and at a young age. Its use when combined with other enzymes and at later stages of growth needs further investigation. Feed additives in experiment 4 prevented the negative effects of CP as the treated chickens did not have lesions on their villus surfaces. The conditions under which these trials were conducted appeared to be such that little benefit was derived from the use of any of the feed additives used. It is possible that under less-hygienic conditions such as those in commercial operations greater benefits from these additives may be realised. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.

Two experiments were conducted for this thesis, to determine whether an animal should be fed to its genetic potential in spite of this not being achievable due to an on-farm constraint. The first experiment was designed to compare the response of pigs housed either individually or in groups to a range of feeds limiting in lysine between 40 and 85 kg live weight. Two hundred and eighty-eight entire male Large White x Landrace pigs were used. The experiment was divided into two growth periods, i.e. from 40 to 60 kg and from 60 to 85 kg. In each period, pigs were subjected to feed containing one of four dietary lysine concentrations. In Period 1, the lysine concentrations were 11.03 (L1); 9.54 (L2); 8.00 (L3) and 6.51 (L4) g/kg, while in Period 2 these were 7.82 (T1); 6.71 (T2); 5.55 (T3) and 4.40 (T4) g/kg. Pigs fed an L1, L2, L3 or L4 diet in Period 1 were fed a T1, T2, T3 and T4 diet in Period 2, respectively. Three buildings provided the following group sizes and floor-space allowances: House 1 contained eight pigs per pen at 1.94 m2/pig; House 2 contained four or eight pigs per pen at 1.72 or 0.86 m2/pig; and House 3 contained one pig per pen at 1.72 m2/pig. The individually-housed pigs were divided into three feeding levels, i.e. ad libitum, or pair-fed so that feed intakes would match those of ad libitum-fed pigs housed in groups of either 4 (restricted-4) or 8 (restricted-8) pigs per pen in House 2. For all group sizes, feed intake increased linearly as the dietary lysine content increased. However, this increase was significantly lower for 8, when compared with 1 and 4 pigs per pen. The linear increase in feed conversion efficiency with dietary lysine content was similar for all group sizes. However, at any dietary lysine concentration, pigs housed in groups of 8 had significantly higher efficiencies than the pigs housed individually or in groups of 4. Average daily gain increased linearly as lysine intake increased, this increase being the same for all group sizes. However, pigs in smaller groups grew significantly faster than those in larger group sizes for any lysine intake. Protein and lysine retention were unaffected by group size, increasing linearly as lysine intake increased. The efficiency of lysine utilisation (0.45) was not impaired by group size. The pair-fed pigs housed individually (restricted-4 and -8) consumed significantly less feed than the individually-housed pigs fed ad libitum, and this was reflected in their average daily gains, which increased linearly as lysine intake increased, but with the restricted-8 growing significantly slower than the ad libitum or restricted-4 pigs. In all three treatments feed conversion efficiency increased linearly with dietary lysine content, although the restricted-4 and -8 had significantly higher efficiencies than the ad libitum-fed pigs at any dietary lysine content. Protein and lysine retentions were unaffected by feeding level and increased significantly with lysine intake. However, at any lysine intake the restricted-8 pigs had a significantly lower efficiency of lysine utilisation than the ad libitum or restricted-4 pigs. The pigs with floor-space allowances of 0.86 and 1.94 m2/pig consumed significantly less and grew slower than the pigs with floor-space allowances of 1.72 m2/pig at any dietary lysine content. Feed conversion efficiency was unaffected by floor-space allowance and increased significantly with dietary lysine content. Similarly, protein and lysine retentions were unaffected by floor-space allowance and increased linearly as lysine intake increased. The efficiency of lysine utilisation (0.45) remained unaffected by floorspace allowance. It was concluded that when animals are socially stressed, feeding according to the requirement for maximum protein growth produces the best biological performance and carcass composition, with the corollary that, if profitability and biological efficiency is to be maximised, pigs housed in stressful conditions, or those whose future performance is predicted to be below potential because of external stressors, should not be given feed of an inferior quality. The second experiment was designed to determine the extent to which grouping or floorspace allowance would alter the nutrient content of feed chosen by pigs given a choice of two feeds differing in protein: energy ratio between 40 to 85 kg live weight. Three hundred and eighteen entire male Large White x Landrace pigs were used. Two buildings provided the following group sizes and floor-space allowances: House 1 contained nine and eighteen pigs per pen at 1.72 or 0.86 m2/pig; House 2 contained four, nine and fourteen pigs per pen at 1.72; 0.86 or 0.49 m2/pig. Animals were given simultaneous ad libitum access to a high (236 g protein/kg as fed) and a low crude protein feed (115 g protein/kg as fed) in two hardened plastic self-feeder bins placed side-by-side. A training period of six days was used prior to the start of the trial, during which the two feeds were alternated daily. The reduction in the proportion of high protein feed chosen over time was significantly higher for the groups of four and eight, in comparison to the groups of nine and eighteen, contrasting with the steady increase for the groups of fourteen pigs. Similarly, the significant increase for pigs with floor-space allowances of 0.49 m2/pig differed from the significant decrease for pigs with floor-space allowances of 0.86 and 1.72m2/pig. Pigs housed in larger group sizes and smaller floor-space allowances consumed significantly less and grew slower than pigs housed in smaller group sizes and larger floorspace allowances. However, the feed conversion efficiency remained unaffected by group size and floor-space allowance. The non-significant effect on protein retention with increasing group size contrasted with the significant increase associated with increasing floor-space allowance. The results of the two studies were compared to determine whether pigs chose differently depending on the degree of stress and the implication of this choice. Average daily gain was significantly reduced as the group size increased for pigs fed a fixed lysine content and choice-fed. However, this reduction was less severe with choice-feeding than when feeding a fixed lysine content. Increasing the group size significantly reduced the feed intake in pigs fed a fixed lysine content only. The efficiency of protein utilisation remained unaffected as the group size increased for the pigs fed a fixed lysine content. However, at any group size pigs fed lower lysine contents had higher efficiencies than pigs fed higher lysine contents. On the contrary, increasing the group size significantly increased the efficiency of protein utilisation in choice-fed pigs. The average daily gain and feed intake was significantly improved as the floor-space allowance increased but was similar for pigs fed a fixed lysine content and choice-fed. Although the efficiency of protein utilisation remained unaffected by increasing the floor-space allowance for the pigs fed a fixed lysine content and pair-fed, at any floor-space allowance pigs fed higher lysine contents had higher efficiencies than pigs fed lower lysine contents. The results indicate that providing socially stressed pigs a choice between an appropriate pair of feeds differing in protein: energy ratio, does not overcome the reduction in potential growth, but does result in performance similar to that of pigs fed a fixed lysine content. It was concluded that the social stress of grouping or floor-space allowance has no influence on the ability of the animal to select an appropriate dietary combination allowing the expression of potential growth within the constraint(s) of the production system. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2005.

African horse sickness (AHS) is a viral disease with high mortality rates, vectored by the Culicoides midge and affecting members of the Equidae family. AHS is endemic to South Africa, and, as a result, affects export and international competitiveness in equine trade, and impacts significantly on the South African racehorse and performance horse industries. AHS also has devastating consequences for rural and subsistence equine ownership. The protocol developed in this dissertation has the potential to serotype and confirm the AHS virus within a few hours at significantly less cost than current methods. It will ease the financial and time constraints of studying an outbreak in real time and has the potential to solve many of the unknown factors surrounding AHS, particularly and most importantly, the role that each serotype plays in outbreaks and the form of the disease contracted by horses. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2009.

Feeding of animal has become increasingly a big challenge for smallholder farmers in the dry season in most of Sahelian countries. Sheep has an important role in social and economical activity of smallholder farmers. Millet stover (MS) and groundnut haulms (GH) are the main crop residues used. Among concentrates, millet bran (MB) and wheat bran (WB) are mostly used by smallholder farmers in feeding animals. However, these crop residues and concentrates are used inadequately. MS is a poor quality roughage diet, legume residues such as GH and brans are rich in nutritive value. The general objective of this study was to develop a feeding technology transferable to smallholder farmers by supplementing MS with GH, MB and WB to increase the growth performance of sheep during the dry season. The first study evaluated the effect of chopping millet stover (Pennisetum glaucum L. R. Br.) in sheep feeding and the degradability in sacco of feeds. Two Holstein cows were used to determine the degradability in sacco of feeds. The potential dry matter degradability (g/kg) were 846, 809, 730, 410, 550 and 370 for millet bran (MB), wheat bran (WB), groundnut haulms (GH), millet stover, leaves plus sheath and stems of MS respectively. The potential nitrogen degradability (g/kg) of MB, WB and GH were 865, 908 and 817 respectively. The nitrogen content was 7.4, 15.1, 22.0 and 26.2 g/kg for MS, GH, millet bran and wheat bran respectively. The effects of chopping millet stover (MS) on dry matter intake in Oudah bicolor sheep were studied in Maradi, Niger. Four treatments were T0 (unchopped MS), T1 (MS chopped, 50 cm), T2 (MS chopped, 25 cm) and T3 (MS chopped, 10 cm). A randomized block design was applied on 36 sheep, implying 9 sheep per treatment (T0, T1, T2 and T3). The treatment T2 or 25 cm chopping size gave the best MS intake of 560 g/day. The second study evaluated the effects of supplementing millet stover (MS) with groundnut haulms (GH), millet bran (MB) and wheat bran (WB). These supplements can improve the feed value of MS and promote live weight gain of sheep in the dry season. The purpose of this study was to develop a feeding technology transferable to smallholder farmers by supplementing MS. The study was conducted in two successive phases. The phase 1 of 66 days, evaluated the effect of GH, MB and WB supplementations on MS intake and growth performance of sheep, the phase 2 of 14 days, assessed the effect of GH, MB and WB supplementations on intake and digestibility of MS. Six treatments were constituted, namely, 1, 2, 3, 4, 5 and 6 formed by four levels of GH (0, 200, 400, 600g) and two others (600g GH + 100g MB) and (600g GH + 75 g WB) respectively. A randomized block design was applied on 36 Oudah bicolor sheep, implying 6 male lambs per treatment (1, 2, 3, 4, 5 and 6). The GH supplementation levels had a linear effect (P<0.001) on dry matter intake (DMI) of MS, cell wall and nitrogen. Millet stover intake (MSI) decreased significantly with increasing level of GH. However the total dry matter intake (TDMI) significantly increased with GH levels. MB increased (P<0.05) both TDMI, TOMI and nitrogen intake (NI), (P<0.001). WB increased MSI (P<0.05), TDMI and TOMI (P<0.01), NI (P<0.001) and cell wall intakes. GH supplementation had both a linear and quadratic effect on DM digestibility of MS, cell wall and nitrogen digestibility. GH had a linear and significant effect (P<0.001) on live weight gain (LWG) and efficiency. The maximum average daily gain (80.5 g/day) was obtained with treatment 6 followed by the treatment 5 (68 g/day). The animals of treatment 1 received MS stover alone and lost their weight (-19.13 g/day). It is concluded that groundnut haulms, millet bran and wheat bran had good nutritive value whereas millet stover had low nutritive value. The 25 cm chopping size was the one to propose to smallholder farmers. The supplementation of MS as the basal diet with GH and brans enhanced sheep production through improvements in digestibility and intakes of TDM, TOM, cell wall and nitrogen. Key words: Millet stover; groundnut haulms; millet bran; wheat bran, chopping; intake; degradability; growth performance; Oudah bicolor sheep. / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

Fertility of Broiler Breeders (BBs) has been researched over many years with inconclusive findings. Genetic selection and improvements in growth traits have had negative effects on fertility of BB. One explanation is related to overweight birds, resulting in the inability of birds to copulate successfully, with no cloacal contact. The requirements of CP for egg production in BB females have been quantified, but there is less literature on how CP affects fertility in male BBs. The objective of this experiment was to investigate the effects of feeding BB males different dietary CP intakes (10.4, 12.4, 14.2, 17.8, 19.3 and 20.1 g CP/bird/day) on fertility in flocks with natural mating and in birds that were artificially inseminated (AI). Results showed that birds consuming 10.4 and 12.4 g CP/bird/day resulted in weight loss (between 24.9 and 23.6% and 26.5 and 22.4% below target BW) over the 29 weeks of the experimental period in both natural mating and individual pens respectively. Intakes of higher protein content (17.8- 20.1 g CP/bird/day) however resulted in BW gain over the experimental period. Hatch percentage across all CP intakes throughout the duration of the study was found to be high, ranging between 75- 100 and 80-95 % in AI and naturally mated birds respectively, and not affected by CP intakes. Similar results for predicted fertility using the methods published by Brillard & Antoine (1990) and Wishart (1997) were found; however Brillard & Antoine (1990) is more lenient requiring less outer perivitelline layer (OPVL) sperm/mm2 to pronounce an egg fertile. Improved fertility predictions could have been made by using examination of inner perivitelline layer (IVPL). There was a tendency for a superior response in predicted fertility from birds with a protein intake of 14.2 g CP/bird/day. This intake also least affected the BW of the male birds and thus 14.2 g CP/bird/day can be recommended for optimal BB male performance. / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.