Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2016-10-20T18:52:30Z No. of bitstreams: 1 2016 - Joyce Mattos de Oliveira.pdf: 2259979 bytes, checksum: dca983fbb22cab52bfa67209c105ae2f (MD5) / Made available in DSpace on 2016-10-20T18:52:30Z (GMT). No. of bitstreams: 1 2016 - Joyce Mattos de Oliveira.pdf: 2259979 bytes, checksum: dca983fbb22cab52bfa67209c105ae2f (MD5) Previous issue date: 2016-03-31 / CAPES / Modern life has diminished the sleep time for the majority of the population, and the consequences of this reduction have been studied both in humans and animal models. In spite of this, only a few studies elucidate the effect sleep deprivation has on the thyroid function, as well as studies on any role exercise might have in the prevention of such alterations. The objective of this study is to assess the protective effect of the strength exercise on the thyroid function in rats that went through paradoxical sleep deprivation for 24 and 96 hours, as well as a rebound sleep for 24 hours. For this study male and female Wistar rats were used (200-250g), submitted to sleep deprivation using the modified multiple platforms, and the isometric exercise was offered by the inverted box proposed by Lac & Cavalie (1999). The animals were distributed in 6 groups: Control (C, males n=8, females = 13); Trained (T, males n=8; females n=13), Trained, with Sleep Deprivation of the paradoxical sleep for 24 and 96 hours (respectively TPSP24 e TPSP96 males n=10; females n=13); Trained with Sleep Deprivation for 24 and 96 hours, plus a rebound sleep for 24 hours (TPSP24R e TPSP96R males n=10; females n=13). All animals went through and adaptation to the strength exercise for 5 days, enduring 5 series of 30 seconds of strength with rest periods of 25 seconds between each series. After adaptation, an extra weight was added to the animal's tail. All animals were killed on the same day and their blood was collected for analysis of T3 (ng/dL), T4 (ug/dL), e TSH (ng/mL) using the radioimmunoassay technique. Ethics committee approval was granted by number UFRRJ N?003/2015. After the statistical analysis we observed a significant body weight loss, both in females and males, and a relative loss in hypophysis weight in males from group T. On the other hand, the relative weight of the adrenal was reduced in the T group of males, and increased in both the T and TP24 groups of females. In males, seric TSH levels have risen with the exercise, normalizing after the deprivation of 24 and 96 hours, and the rebound in the PS96 group. The PSP was able to induce a raise in the T3 level in the groups TP24 and TP96 in males ? no significant alterations were observed in females. As for the seric T4 in males, there was no alteration, although in females the 24 hours PSP was able to rise those values. This study indicates a protective effect by the isometric exercise, preventing TSH and seric T4 and T3 alterations induced by deprivation of the paradoxical sleep. As such more studies are necessary to clarify the mechanisms involved in such protection / A vida moderna tem diminu?do o tempo de sono da maioria da popula??o e as consequ?ncias dessa redu??o t?m sido estudadas em humanos e modelos animais. J? o papel da tire?ide na priva??o de sono associada com exerc?cios de for?a n?o est? bem estabelecido, pois n?o tem sido estudado. Este estudo, no entanto, tem como objetivo avaliar o efeito protetor do exerc?cio de for?a sobre a fun??o tireoidiana em ratos ap?s a priva??o de sono paradoxal (PSP) por 24 e 96 horas assim como o sono rebote de 24 horas. Para a realiza??o deste trabalho, foram utilizados ratos machos e f?meas Wistar (200-250g) submetidos a priva??o de sono pela metodologia das plataformas m?ltiplas modificadas e o exerc?cio isom?trico foi feito pela metodologia da caixa invertida proposta por Lac & Cavalie (1999). Os animais machos foram distribu?dos em 6 grupos: Controle (C n=8 machos; f?meas, n=13); Treinado (T=8 machos; f?meas, n=13); Treinado com Priva??o de sono paradoxal por 24 horas e 96 horas (TPSP24 e TPSP96 n=10, machos; f?meas, n=13); Treinado com Priva??o de sono paradoxal por 24 horas e 96 horas mais per?odo de sono rebote por 24 horas (TPSP24R e TPSP96R n=10, machos; f?meas, n=13). Os animais foram adaptados ao exerc?cio de for?a por 5 dias, onde era constitu?do por 5 s?ries de 30 segundos de for?a com intervalos de descanso por 25 segundos entre as s?ries. Ap?s a adapta??o, foi adicionado um peso extra na cauda desses animais. Todos os animais foram eutanasiados no mesmo dia, o sangue coletado para an?lise de T3 ng/dL, T4 ?g/dL, e TSH ng/mL pela t?cnica de Radioimunoensaio. Aprova??o pelo comit? de ?tica da UFRRJ N?003/2015. Ap?s an?lise, observamos perda do peso corporal tanto nas f?meas quanto nos machos e uma diminui??o no peso relativo da hip?fise apenas nos machos do grupo T. Por outro lado, o peso relativo da adrenal se manteve reduzido no grupo T dos machos e aumentado no grupo T e TP24 das f?meas. Os n?veis s?ricos de TSH nos machos aumentaram com o exerc?cio nos grupos T, normalizando com a priva??o de 24 horas e retornando ao aumento no grupo TP24R. A PSP foi capaz de provocar um aumento nos n?veis de T3 nos grupos TP24 e TP96 dos machos, e nas f?meas n?o foi observado altera??es significativas. Quanto aos valores de T4 nos machos, n?o foi constatado altera??es significativas e nas f?meas a PSP foi capaz de elevar tais valores. Sugerimos que o exerc?cio de for?a esteja contribuindo para a prote??o dos impactos agressivos causados pela priva??o de sono paradoxal na fisiologia end?crina tanto em machos quanto em f?meas.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-03-10T00:02:16Z No. of bitstreams: 1 FernandaFernandesKolodiuk_DISSERT.pdf: 12001033 bytes, checksum: 477763b89fc24705d27960971b414f59 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-16T20:20:28Z (GMT) No. of bitstreams: 1 FernandaFernandesKolodiuk_DISSERT.pdf: 12001033 bytes, checksum: 477763b89fc24705d27960971b414f59 (MD5) / Made available in DSpace on 2016-03-16T20:20:28Z (GMT). No. of bitstreams: 1 FernandaFernandesKolodiuk_DISSERT.pdf: 12001033 bytes, checksum: 477763b89fc24705d27960971b414f59 (MD5) Previous issue date: 2015-05-19 / Demanda acad?mica, novo contexto social, novas rotinas e diminui??o do controle dos pais s?o fatores que podem influenciar o padr?o de sono de estudantes que ingressam na universidade. Os discentes de Medicina da Universidade Federal do Rio Grande do Norte (UFRN) apresentam elevada carga hor?ria, conte?do denso nas disciplinas, as aulas do 1o semestre come?am ?s 7 horas da manh? e sua popula??o ? formada por adultos jovens, que ainda sofrem com o atraso de fase de sono comum na adolesc?ncia, o que indica que o hor?rio de aula pode ser inadequado nesta faixa et?ria. A redu??o do sono noturno durante os dias de aula e a tentativa de recupera??o do sono perdido nos dias livres ? Jet lag social (JLS), sugere que j? no primeiro semestre os estudantes sofrem com press?o do sono, o que pode refletir negativamente em tarefas cognitivas e no desempenho acad?mico. Portanto, o objetivo deste trabalho ? avaliar a rela??o entre a press?o do sono e o perfil acad?mico de estudantes de Medicina do primeiro semestre da UFRN, caracterizando sociodemograficamente esta popula??o e investigando poss?veis reflexos no ritmo de atividade-repouso e no desempenho acad?mico. Participaram desta pesquisa 88 estudantes, saud?veis de ambos os sexos, que respoderam aos seguintes question?rios: Qualidade de Sono de Pittsburgh (IQSP), Escala de Sonol?ncia de Epworth (ESE), Cronotipo Horne & Ostberg (HO), Cronotipo Munique (MCTQ) e ?A Sa?de e o Sono?, adaptado. Actimetria foi utilizada em 14 dias para elabora??o de actogramas e obten??o de vari?veis n?o param?tricas do ritmo de atividade-repouso. A nota da disciplina M?dulos Biol?gicos I foi utilizada como desempenho acad?mico. O JLS foi utilizado como medida de press?o do sono e o n?vel de signific?ncia estat?stica foi 95%. A popula??o ? homog?nea em rela??o aos aspectos sociodemogr?ficos e a maioria tem estilo de vida saud?vel, pratica atividade f?sica, locomove-se at? a universidade em carro e leva entre 15 e 30 minutos para realizar tal percurso. Em rela??o ao CSV, grande parte apresentou cronotipo intermedi?rio e vespertino, necessita cochilar durante a semana, sofre sonol?ncia diurna e apresenta m? qualidade de sono. 83% da amostra tem ao menos 1h de JLS, o que nos levou ? divis?o em dois grupos: Grupo < 2h JLS (N=44) e Grupo ? 2h JLS (N=44). Os grupos diferiram apenas no cronotipo, demonstrando que indiv?duos mais vespertinos apresentam mais JLS. Entretanto, n?o encontramos diferen?as em rela??o aos aspectos sociodemogr?ficos, ritmo de atividade-repouso ou desempenho acad?mico. A homogeneidade da amostra possivelmente dificultou a compara??o entre os grupos, todavia, ? alarmante que os estudantes j? apresentem, no primeiro semestre: JLG, qualidade de sono ruim e sonol?ncia diurna excessiva, os quais podem acentuar-se no decorrer do curso, com o in?cio de plant?es noturnos e o aumento da carga hor?ria. Abordar a import?ncia de bons h?bitos de sono e a mudan?a no hor?rio de in?cio da aula s?o estrat?gias que visam ? melhoria na sa?de dos estudantes. / Academic demands, new social context, new routines and decrease of the parental control, are factors that may influence the sleep pattern of freshman students at the University. Medical students from the Federal University of Rio Grande do Norte (UFRN) have a full-time course, subjects with high-level content, and, at the first semester, classes begin at 7 a.m. This group composed by young adults who still suffering with delayed sleep phase, common in adolescence, indicating that this class schedule can be inappropriate at this age. The reduction of nocturnal sleep during school days, and the attempt to recover sleep on free days ? social jet lag (JLS), suggests that in the first semester, students suffer from high sleep pressure. High sleep pressure may reflect on cognitive tasks and performance. Therefore, the aim of this study was to investigate the relationship between sleep pressure and the academic profile of medical students from the first semester of UFRN, characterizing this population socio-demographically and investigating possible impacts on therestactivity rhytm and academic performance. A sample of 88 students, healthy men and women awswered the following questionnaires: Pittsburgh Sleep Quality (PSQI), Epworth Sleepiness Scale (ESS), Horne & Ostberg Chronotype (HO), Munich Chronotype (MCTQ) and ?Health and Sleep? adapted. Actigraphy was used during 14 days to make actogramas and obtain non-parametric variables of the rest-activity rhythm and the grades of the morning schedule were used as academic performance. The JLS was used as a measure of sleep pressure. Statistics significance level was 95%. The population was sociodemographic homogeneous. Most students have healthy lifestyle, practice physical activity, use car to go to the university and take between 15 and 30 minutes for this route. Regarding CSV, most were classify as intermediate (38.6%) and evening (32%) chronotypes, needs to nap during the week, suffer daytime sleepiness and have poor sleep quality. 83% of the sample has at least 1h JLS, which led us to divide into two groups: Group <2h JLS (N = 44) and Group ? 2h JLS (N = 44). The groups have differences only in chronotype, showing that most evening individuals have more JLS, however, no differences were found in relation to sociodemographic aspect, rest-activity rhythm or academic performance. The homogeneity of the sample was limited to compare the groups, however, is alarming that students already present in the first half: JLG, poor sleep quality and excessive daytime sleepiness, which can be accentuated through the university years, with the emergence of night shifts and increased academic demand. Interventionsaddressingthe importance of good sleep habits and the change of the class start time are strategies aimed to improve student?s health.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-03-10T21:38:12Z No. of bitstreams: 1 LuanaGabrielleDeFrancaFerreira_DISSERT.pdf: 3743895 bytes, checksum: 6586155eff1318ab76c3be9fd110f030 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-17T22:48:47Z (GMT) No. of bitstreams: 1 LuanaGabrielleDeFrancaFerreira_DISSERT.pdf: 3743895 bytes, checksum: 6586155eff1318ab76c3be9fd110f030 (MD5) / Made available in DSpace on 2016-03-17T22:48:47Z (GMT). No. of bitstreams: 1 LuanaGabrielleDeFrancaFerreira_DISSERT.pdf: 3743895 bytes, checksum: 6586155eff1318ab76c3be9fd110f030 (MD5) Previous issue date: 2015-04-10 / Diversos estudos revelam que nas ?ltimas d?cadas ocorreu uma diminui??o na dura??o do sono. Os compromissos sociais, como o trabalho e a escola, muitas vezes n?o est?o alinhados ao ?tempo biol?gico? dos indiv?duos. Somada a isso, observa-se uma menor for?a do zeitgeber causada pela menor exposi??o ? luz durante o dia e maior ? noite. Isso gera um d?bito cr?nico de sono que ? compensado nos dias livres, ocorrendo semanalmente uma restri??o e extens?o do sono denominada de jet lag social. A priva??o de sono vem sendo associada ? obesidade, risco cancer?geno e cardiovascular. Desta-forma, sugere-se que o sistema nervoso auton?mico seja um caminho que relaciona os problemas do sono ?s doen?as cardiovasculares. No entanto, al?m das evid?ncias demonstradas por pesquisas com uso de modelos de priva??o de sono de forma aguda e controlada, s?o necess?rios estudos investigando efeitos da priva??o do sono de forma cr?nica como ocorre no jet lag social. O objetivo deste estudo foi investigar a influ?ncia do jet lag social em marcadores circadianos de atividade-repouso e card?acos em estudantes do Curso de Medicina. Trata-se de um estudo transversal e observacional realizado no Laborat?rio de Neurobiologia e Ritmicidade Biol?gica (LNRB) do Departamento de Fisiologia da UFRN. Participaram da pesquisa estudantes de medicina matriculados no 1? per?odo do curso da UFRN. Foram utilizados os seguintes instrumentos: Question?rio cronotipo de Munique (MCTQ); Question?rio para identifica??o de indiv?duos matutinos e vespertinos (MEQ ou HO); ?ndice de qualidade do sono de Pittsburgh; Escala de Sonol?ncia de Epworth; Act?metro; Cardiofrequenc?metro. Foram analisadas vari?veis de caracteriza??o do sono, n?o param?tricas (IV60, IS60, L5 e M10) e ?ndices card?acos no dom?nio do tempo, frequ?ncia (LF, HF, LF/HF) e n?o linear (SD1, SD2, SD1/SD2). Realizou-se an?lise estat?stica descritiva, comparativa e de correla??o com uso do programa SPSS vers?o 20. Participaram do estudo 41 estudantes, 48,8% (20) mulheres e 51,2% (21) homens, com 19,63 ? 2,07 anos. O jet lag social teve uma m?dia de 02:39h ? 00:55h, 82,9% (34) com jet lag social ? 1 hora e houve correla??o negativa com escore cronotipo de Munique evidenciando maior priva??o do sono em indiv?duos com tend?ncia ? vespertinidade. Qualidade do sono ruim foi detectada em 90,2% (37) (X 2 = 26,56, p < 0,001) e 56,1% (23) sonol?ncia diurna excessiva (X 2 = 0,61, p = 0,435). Observou-se diferen?a significativa dos valores de LFnu, HFnu e LF/HF entre os grupos de jet lag social < 2h e ? 2h e houve correla??o do jet lag social com LFnu (rs = 0,354, p = 0,023), HFnu (rs = - 0,354, p = 0,023) e LF/HF (rs = 0,355, p = 0,023). Verificou-se ainda associa??o negativa entre IV60 e ?ndices no dom?nio do tempo e n?o lineares. Sugere-se que a priva??o cr?nica de sono pode ter associa??o com maior atividade simp?tica promovendo aumento no risco cardiovascular. / Studies reveal that in recent decades a decrease in sleep duration has occurred. Social commitments, such as work and school are often not aligned to the "biological time" of individuals. Added to this, there is a reduced force of zeitgeber caused by less exposure to daylight and larger exposure to evenings. This causes a chronic sleep debt that is offset in a free days. Indeed, a restriction and extent of sleep called "social Jet lag" occurs weekly. Sleep deprivation has been associated to obesity, cancer, and cardiovascular risk. It is suggested that the autonomic nervous system is a pathway that connects sleep problems to cardiovascular diseases. However, beyond the evidence demonstrated by studies using models of acute and controlled sleep deprivation, studies are needed to investigate the effects of chronic sleep deprivation as it occurs in the social jet lag. The aim of this study was to investigate the influence of social jet lag in circadian rest-activity markers and heart function in medical students. It is a cross-sectional, observational study conducted in the Laboratory of Neurobiology and Biological Rhythmicity (LNRB) at the Department of Physiology UFRN. Participated in the survey medical students enrolled in the 1st semester of their course at UFRN. Instruments for data collection: Munich Chronotype Questionnaire, Morningness Eveningness Questionnaire of Horne and ?stberg, Pittsburgh Sleep Quality Index, Epworth Sleepiness Scale, Actimeter; Heart rate monitor. Analysed were descriptive variables of sleep, nonparametric (IV60, IS60, L5 and M10) and cardiac indexes of time domain, frequency (LF, HF LF / HF) and nonlinear (SD1, SD2, SD1 / SD2). Descriptive, comparative and correlative statistical analysis was performed with SPSS software version 20. 41 students participated in the study, 48.8% (20) females and 51.2% (21) males, 19.63 ? 2.07 years. The social jet lag had an average of 02: 39h ? 00:55h, 82.9% (34) with social jet lag ? 1h and there was a negative correlation with the Munich chronotype score indicating greater sleep deprivation in subjects prone to eveningness. Poor sleep quality was detected in 90.2% (37) (X2 = 26.56, p <0.001) and 56.1% (23) excessive daytime sleepiness (X2 = 0.61, p = 0.435). Significant differences were observed in the values of LFnu, HFnu and LF / HF between the groups of social jet lag <2h and ? 2h and correlation of the social jet lag with LFnu (rs = 0.354, p = 0.023), HFnu (rs = - 0.354 , p = 0.023) and LF / HF (r = 0.355, p = 0.023). There was also a negative association between IV60 and indexes in the time domain and non-linear. It is suggested that chronic sleep deprivation may be associated with increased sympathetic activation promoting greater cardiovascular risk.