Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-05-12T11:13:08Z No. of bitstreams: 1 2016 - Michele Rodrigues Francisco.pdf: 1285744 bytes, checksum: 598c2fe8a98038b9745debaf13625959 (MD5) / Made available in DSpace on 2017-05-12T11:13:08Z (GMT). No. of bitstreams: 1 2016 - Michele Rodrigues Francisco.pdf: 1285744 bytes, checksum: 598c2fe8a98038b9745debaf13625959 (MD5) Previous issue date: 2016-05-20 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Trichoderma spp. They are not pathogenic fungi and develop in substrates and diverse environmental conditions. Identification based only on morphological characteristics is considered complex, due to the genetic diversity of the species. Therefore, the taxonomy of the genus Trichoderma is performed with the aid of other analyzes such as biochemical and molecular changes. Some species are capable of producing high amounts of extracellular enzymes involved in the hydrolysis of polysaccharides are thus considered fungi with high potential in the production of hydrolytic enzymes. The aim of this study was to select and identify the species of the genus Trichoderma spp. And evaluate the production of industrial enzymes these isolates. They identified 58 isolates of Trichoderma spp fungi. soil and plants by morphological and molecular analysis and was also evaluated the enzymatic production capacity. Enzyme technology is a promising area in new technologies for synthesis of compounds with high added value, where biocatalisados industrial processes have a lower environmental impact, lower energy cost, because the enzymes are biodegradable substances and have specificity to minimize the undesirable effects. In this work, we used PDA culture medium for development of fungi. Morphological identification is achieved using the identification key according to Samuels et al. (2015) Molecular and held by DNA extraction from the mycelium of fungi in accordance with CTAB protocol. For enzyme analysis, qualitative assays were performed, used specific means of solid culture to evaluate the production of hydrolytic enzymes: Where the culture media containing as a substrate for development and enzyme production of Trichoderma spp. skimmed milk production peptidase; soluble starch for the production of amylase, cellulase production carboxymethylcellulose, citrus pectin for the production of pectinase and yeast extract, dextrose, ferrous sulfate, ammonium, magnesium, manganese and potassium chloride for the production of phytase. According to the results, the morphological analysis identified 4 species such as T. atroviride, T .asperelloide, T. harzianum, T. longibrachiatum, these species were verified by molecular analysis. In relation to the enzyme potential, the result was negative for the activity of peptidase enzymes, cellulase and pectinase and positive for amylase, and the activity of the enzyme phytase, only an isolated was positive, this was identified according to morphological analysis as It is the species T. longibrachiatum / Os Trichoderma spp. n?o s?o fungos patog?nicos e se desenvolvem em diversificados substratos e condi??es ambientais. Sua identifica??o baseada apenas em caracter?sticas morfol?gicas ? considerada complexa, por conta da diversidade gen?tica das esp?cies. Portanto, a taxonomia do g?nero Trichoderma ? realizada com o aux?lio de outras an?lises, tais como bioqu?micas e moleculares. Algumas esp?cies s?o capazes de produzir elevadas quantidades de enzimas extracelulares envolvidas na hidr?lise de polissacar?deos, sendo assim considerados fungos com alto potencial na produ??o de enzimas hidrol?ticas. O objetivo deste estudo foi selecionar e identificar as esp?cies do g?nero Trichoderma spp. e avaliar a produ??o de enzimas industriais destes isolados. Foram identificados 58 isolados de fungos Trichoderma spp. de solo e plantas por an?lise morfol?gica, molecular e tamb?m foi avaliado a capacidade de produ??o enzim?tica. A Tecnologia enzim?tica ? uma ?rea promissora dentro das novas tecnologias para s?ntese de compostos que possuem alto valor agregado, onde os processos industriais biocatalisados apresentam menor impacto ambiental, menor custo de energia, pois as enzimas s?o subst?ncias biodegrad?veis e possuem especificidade que minimizam os efeitos indesej?veis. Neste trabalho, foi utilizado meio de cultura BDA para desenvolvimento dos fungos. A identifica??o morfol?gica foi realizada com aux?lio da chave de identifica??o de acordo com Samuels et al. (2015) e molecular, realizada atrav?s da extra??o de DNA do mic?lio dos fungos, de acordo com protocolo CTAB. Para as an?lises enzim?ticas, foram realizados ensaios qualitativos, utilizado meio de cultura s?lido espec?fico para avaliar a produ??o de enzimas hidrol?ticas: Onde os meios de cultura continham como substrato para desenvolvimento e produ??o enzim?tica dos isolados de Trichoderma spp. leite desnatado para produ??o de peptidase; amido sol?vel para produ??o de amilase, carboximetilcelulose para produ??o de celulase, pectina c?trica para produ??o de pectinases e extrato de levedura, dextrose, sulfato ferroso, am?nio, magn?sio, mangan?s e cloreto pot?ssio para produ??o de fitase. De acordo com os resultados, a an?lise morfol?gica identificou 4 esp?cies, tais como: T. atroviride, T. asperelloide, T. harzianum, T. longibrachiatum, estas esp?cies foram confirmadas por an?lise molecular. J? com rela??o ao potencial enzim?tico, o resultado foi negativo para a atividade das enzimas peptidase, celulase e pectinase e positivo para amilase, e para a atividade da enzima fitase, somente um isolado apresentou resultado positivo, este foi identificado de acordo com an?lise morfol?gica como sendo da esp?cie T. longibrachiatum