Thesis (M.S.)--University of Missouri--Columbia, 2004. / "July 2004" Typescript. Includes bibliographical references (l. 64-66). Also issued on the Internet.

Thesis (M.S.)--University of Missouri--Columbia, 2004. / Typescript. Includes bibliographical references (leaves 64-66). Also issued on the Internet.

Made available in DSpace on 2014-06-11T19:22:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-02-16Bitstream added on 2014-06-13T20:48:47Z : No. of bitstreams: 1 rodrigues_mmp_me_botfmvz.pdf: 498195 bytes, checksum: c6d0362fac624d7b6051d886cab51a18 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / As lesões prostáticas em cães vêm sendo cada vez mais estudadas, uma vez que esta espécie é considerada modelo experimental para afecções prostáticas no homem. As principais são a hiperplasia prostática benigna (HPB), prostatites, cistos e o adenocarcinoma. Recentemente tem se dado atenção às lesões pré-neoplásicas, dentre elas a neoplasia intra-epitelial prostática (PIN) e a atrofia inflamatória proliferativa (PIA). Alguns marcadores imunoistoquímicos são utilizados com o objetivo de avaliar o desenvolvimento de câncer prostático a partir de lesões pré-neoplásicas. A cicloxigenase-2 (COX-2) apresenta papel fundamental na resposta inflamatória e, esta pode ter relação com o desenvolvimento do câncer, além de induzir estímulos mitogênicos, enquanto que o fator de crescimento transformador-ß (TGF-ß) induz a angiogênese e inibe a proliferação celular, sendo considerado um mediador do crescimento prostático. No presente trabalho estes marcadores foram utilizados com o objetivo de correlacionar lesões não neoplásicas, pré-neoplásicas e neoplásicas. Os resultados obtidos mostram que as neoplasias e as lesões pré-neoplásicas expressam grande quantidade de COX-2 e TGF-ß, o que permite afirmar o papel destas proteínas no desenvolvimento do câncer prostático em cães. / Prostatic lesions in dogs are being studied more and more, since the dogs are considered na experimental model for prostatic diseases in humans. The main prostatic diseases are benign prostatic hyperplasia (BPH), prostatic inflammation, cysts and adenocarcinoma. Pre neoplastic lesions are being studied, among them prostatic intra epithelial neoplasia (PIN) and prostatic inflammatory atrophy (PIA). Immunohistochemistry markers are used to evaluate prostatic cancer from pre neoplastic lesions. COX-2 has the main role in inflammatory response and can be involved in cancer development, because induces mitosis, and TGF-ß) induces angiogenesis and inhibits cellular proliferation, and is considered a prostatic growth mediator. In this study, these markers were used to correlate non neoplastic lesions, pre neoplastic lesions and prostatic cancer. Our results show that neoplastic and pre neoplastic lesions express high amounts of COX-2 and TGF-ß, witch allows us to confirm the involvement of these proteins in prostatic cancer development in dogs.

Includes bibliographical references and index.

Orientador: Renée Laufer Amorim / Banca: Veridiana Maria Brianezi Dignani De Moura / Banca: Sérgio Luis Felisino / Resumo: As lesões prostáticas em cães vêm sendo cada vez mais estudadas, uma vez que esta espécie é considerada modelo experimental para afecções prostáticas no homem. As principais são a hiperplasia prostática benigna (HPB), prostatites, cistos e o adenocarcinoma. Recentemente tem se dado atenção às lesões pré-neoplásicas, dentre elas a neoplasia intra-epitelial prostática (PIN) e a atrofia inflamatória proliferativa (PIA). Alguns marcadores imunoistoquímicos são utilizados com o objetivo de avaliar o desenvolvimento de câncer prostático a partir de lesões pré-neoplásicas. A cicloxigenase-2 (COX-2) apresenta papel fundamental na resposta inflamatória e, esta pode ter relação com o desenvolvimento do câncer, além de induzir estímulos mitogênicos, enquanto que o fator de crescimento transformador-ß (TGF-ß) induz a angiogênese e inibe a proliferação celular, sendo considerado um mediador do crescimento prostático. No presente trabalho estes marcadores foram utilizados com o objetivo de correlacionar lesões não neoplásicas, pré-neoplásicas e neoplásicas. Os resultados obtidos mostram que as neoplasias e as lesões pré-neoplásicas expressam grande quantidade de COX-2 e TGF-ß, o que permite afirmar o papel destas proteínas no desenvolvimento do câncer prostático em cães. / Abstract: Prostatic lesions in dogs are being studied more and more, since the dogs are considered na experimental model for prostatic diseases in humans. The main prostatic diseases are benign prostatic hyperplasia (BPH), prostatic inflammation, cysts and adenocarcinoma. Pre neoplastic lesions are being studied, among them prostatic intra epithelial neoplasia (PIN) and prostatic inflammatory atrophy (PIA). Immunohistochemistry markers are used to evaluate prostatic cancer from pre neoplastic lesions. COX-2 has the main role in inflammatory response and can be involved in cancer development, because induces mitosis, and TGF-ß) induces angiogenesis and inhibits cellular proliferation, and is considered a prostatic growth mediator. In this study, these markers were used to correlate non neoplastic lesions, pre neoplastic lesions and prostatic cancer. Our results show that neoplastic and pre neoplastic lesions express high amounts of COX-2 and TGF-ß, witch allows us to confirm the involvement of these proteins in prostatic cancer development in dogs. / Mestre

Σκοπός της παρούσας εργασίας ήταν η δημιουργία ενός ευφυούς συστήματος για τη διάγνωση νοσημάτων του προστάτη αδένα με βάση σημεία και συμπτώματα που διαπιστώνονται σε έναν ασθενή όπως καταγράφονται κατά την κλινική του εξέταση. Τα βασικά σημεία της εργασίας είναι τα εξής: αρχικά παράγεται το μοντέλο της διαγνωστικής στρατηγικής που ακολουθείται για τη διάγνωση των συγκεκριμένων παθήσεων και στη συνέχεια γίνεται εκμαίευση της διαγνωστικής γνώσης από ειδικούς ή/και από εμπειρικά δεδομένα. Η διαδικασία και η γνώση αναπαρίστανται με χρήση υβριδικών μεθόδων αναπαράστασης και με τη χρήση ασάφειας ή/και αβεβαιότητας, η οποία είναι εγγενής σε τέτοια διαγνωστικά συστήματα. Το σύστημα αναπτύχθηκε με τέτοια εργαλεία που δίνουν την δυνατότητα στέγασής του σε ιστότοπο ώστε να χρησιμοποιηθεί ως ειδικός συμβουλευτικός οδηγός από ενδιαφερομένους μη ειδικούς ιατρούς & από ασθενείς καθώς και από φοιτητές υγειονομικών σχολών για εκπαιδευτική χρήση. Το σύστημα περιέχει γνώση εμπειρογνωμόνων σε συνδυασμό με στοιχεία πλούσιας βιβλιογραφικής αναζήτησης και μελέτης. Τέλος πραγματοποιήθηκε επικύρωση των αποτελεσμάτων μέσα από μια τυχαιοποιημένη κλινική έρευνα. Η υλοποίηση του συστήματος μας στηρίζεται σε ένα ειδικό κέλυφος δημιουργίας ευφυών συστημάτων και σε εργαλεία μεθόδων μηχανικής μάθησης. Επίσης, υλοποιούνται δύο εκδοχές (Hirofilos Ι & ΙΙ) και γίνεται σύγκριση των απο-τελεσμάτων. Αναγνωρίστηκε η σαφής υπεροχή του Hirofilos ΙΙ που αναπτυχθηκε με σύστημα μηχανικής εκμάθησης και με δυνατότητα διαχρονικής αναβάθμισης. / During making this project our concern was the creation of a new expert system for diagnosis prostate disease depending on the clinical status of a patient as these are identified during medical examination. The main points were as follows: First has been constructed a diagnosis process model which was followed by the diagnosis for the most common prostate diseases. Secondly was implemented extracting of the medical knowledge based on specialists or/and experimental data. The whole process and the medical Knowledge will be developed using hybrid methods of representation and having the partcipation of such saying fuzziness or inaccuracy, that is innate in that kind of intelligent decision-support. The new intelligent system is already accommodated on a hospital server and is used as a decision-support system, as well as an e-learning platform for medical students. For the implementation of the new system have been used an expert system shell and knowledge engineering tools.