Falls are a major contributor to construction-related fatalities. Many construction fall fatalities occur during roofing, and may be prevented by proper use of fall protection. A prevalent option for fall protection is a personal fall arrest system (PFAS). However, full adaption of PFAS is hindered by usability issues, particularly in the harness component. Current research aims to develop design requirements for more usable fall arrest harness. A study was conducted to consider the inter-relations of objective measures of fit and strap pressure, along with subjective usability measures including discomfort ratings and post-task questionnaire responses. 18 local roofers were recruited to test three different harnesses, while performing both quiet calibration-oriented trials and simulated roofing tasks. Significant correlations between discomfort ratings and pressure values were found only in quiet trials. Questionnaire responses were validated by inter-correlations and by significant correlations with discomfort ratings. Multiple comparisons of objective fit values and questionnaire responses revealed deficits in the low-end harness, while suggesting few differences between the mid- and high-range harnesses. Results from analysis of both objective and subjective measures were considered alongside free-response prompts to develop a set of four requirements for consideration in future harness design, including a lowered harness weight, an intermediate level of padding, inclusion of rolling style vertical strap quick-adjusters, a belt-style thigh strap adjustment mechanism, and the adoption of a three-sized sizing scheme. / Master of Science

Prohibition of diskrimination, constitutional legal level of protection of the rights of ethnic minorities against discrimination Abstract The thesis deals with the concept of discrimination from a theoretical and practical point of view and focuses closely on the issue of ethnic minorities and racism. To clarify the whole issue, the work is presented by the theoretical inclusion of anti-discrimination law in the legal framework of human rights law in both the Czech Republic and the global framework in the form of international agreements. It deals with ideological sources, including the historical origin of law related to discrimination. It also contains a definition of basic concepts, which are necessary to clarify this complex phenomenon. It thematically defines both the legal regulation of individual protection of the individual and the collective concept of protection against discrimination and the rights of ethnic minorities as a whole. The main part of this work refers about case law in the field of discrimination on the grounds of ethnicity or nationality and all other forms of protection against illegal exclusion or segregation. It emphasizes in law the exceptional instrument of reversing the burden of proof, which makes it easier to prove a potentially discriminated party to a dispute. The thesis...

Cell wall is the major barrier in the delivery of biomolecules such as nucleic acids into the plant cell. Biological (bacteria or viruses) and biolistic (particle-based) methods are used to deliver nucleic acids into the plant cell. However, these methods have significant limitations when it comes to species range, scalability, and field assays. In this work, we report the use of layered double hydroxide (LDH) topically applied to deliver RNA molecules into the plant cell. LDH were assembled by methanol-based co-precipitation of magnesium and aluminum nitrate solution with sodium hydroxide and finally dispersed in deionized water. The assembled LDH were physically characterized by AFM, zeta-sizer and their binding to RNA was confirmed by gel electrophoresis. LDH complexed with double stranded RNA (dsRNA) was topically applied to Nicotiana benthamiana leaves. As a model system, virus specific dsRNA-LDH complexes were used to activate cellular RNAi machinery against Tomato Yellow leaf Curl Virus (TYLCV) in N. benthamiana plants. Our results demonstrated that topical application of the TYLCV specific dsRNA-LDH complexes reduce viral genome accumulation and viral symptoms development. Similarly, dsRNA-LDH protected plants produce typical leaves, flowers, and seeds, confirming efficient virus resistance compared unprotected TYLCV infected plants. Topical application and noninvasive delivery of nucleic acid has several advantages, as these methods are specie independent, easy to scale up, applied with low-pressure spray, requires no tissue culture and no sophisticated equipment. The LDH based noninvasive delivery of nucleic acids has the capability to overcome the cell wall barrier limitations and will open new opportunities to exploit the full potential of cellular machinery to produce resilient plants and insure sustainable food production.

Supervisory control and data acquisition systems are extensively used in the critical infrastructure domain for controlling and managing large-scale industrial applications. This thesis presents a security management structure developed to protect ICS networks from security intrusions. This structure is formed by a combination of several modules for monitoring system-utilization parameters, data processing, detection of known attacks, forensic analysis to support against unknown attacks, estimation of control system-specific variables, and launch of appropriate protection methods. The best protection method to launch in case of an attack is chosen by a multi-criteria analysis controller based on operational costs and efficiency. A time-series ARIMA model is utilized to estimate the future state of the system and to protect it against cyber intrusions. Signature and performance based detection techniques assist in real-time identification of attacks with little or no human intervention. Simulation results for Scanning, Denial of Service and Injection attacks are provided.

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Much of southern Ontario relies on groundwater in fractured rock aquifers as a municipal drinking water source, thus the vulnerability of these sources is of importance from public health, economic, and environmental perspectives. Aquifer vulnerability assessments serve as visual communication tools useful in efficiently allocating resources for the establishment of new drinking water sources, hydrogeological characterization, and source water protection planning decisions. Examples of current vulnerability assessments include: DRASTIC, GOD, EPIK, AVI, COP and ISI. These vulnerability assessment methods either fail to quantitatively incorporate characteristics of fractured rock and preferential pathways, or they account for only heavily karstified areas; none are suited to the fractured rock formations in Ontario. The goal of this work is to incorporate fractured rock characteristics in a new aquifer vulnerability assessment method using readily attainable quantitative data to produce an inexpensive and straightforward regional aquifer vulnerability map highlighting hydrogeological areas that are more fundamentally prone to contamination than others. This proposed method is applied to the Acton-Georgetown study area in southern Ontario, along with the AVI and DRASTIC methods for comparison. The AVI and DRASTIC vulnerability assessments yield very different results from each other, and the proposed method demonstrates the heavy influence that fractured rock has on the vulnerability of the study area. The heterogeneity of variables used in some of the methods created difficulty in the interpolation of point data, rendering the use of generalized spatial data more valuable. These results and the corresponding limitations and recommendations for future improvements are discussed in light of these conclusions. / Thesis / Master of Applied Science (MASc)

Previous studies by our group showed that vaccinia virus recombinants expressing Brucella abortus (BA) antigens heat shock protein GroEL, 18 kDa protein and Cu/Zn SOD, were unable to induce protective immune responses against Brucella challenge. This dissertation analyzes the possible reasons for this phenomenon, by using other genes/proteins from BA and Listeria monocytogenes (LM), various shuttle plasmids (pSC65, pSC11) and immune response modulators (CpG, IL-12, B7-1). As the first objective, a vaccinia virus recombinant (WRL7/L12), expressing the BA L7/L12 gene was generated. L7/L12 ribosomal protein was used as a T-cell reactive antigen, with protective potential to Brucella challenge. The WRL7/L12 was able to express the gene of interest and induce IgG2A type antibody response, but not a protective immune response against Brucella challenge. As a control, an antigen from LM proven to induce CTL and protective immune responses, was used to test the efficacy of vaccinia virus to induce protection. A portion of hly gene, encoding partial listeriolysin (pLLO), was inserted into the same vaccinia virus stain. This recombinant (WRpLLO) was able to induce protection against a Listeria challenge. Next another vaccinia virus recombinant expressing Brucella abortus Cu/Zn SOD was analyzed. Although a variety of approaches, including the enhancement of the protein expression by the pMCO2 synthetic promoter, booster immunization, addition of the oligomer CpG adjuvant (WRSODCpG) to enhance Th1 type response, were used, the SOD recombinant failed to protect mice against Brucella challenge. Lastly, vaccinia virus produces a family of proteins that bind cytokines, chemokines and interferons to evade the host defensive systems. Therefore, a vaccinia virus strain co-expressing murine IL-12, and cofactor B7-1, were used to generate the recombinant WRIL12L7/L12. In order to further boost the induction of Th 1 type response, the adjuvant CpG was used. A similar recombinant, WRIL12pLLO, was generated with partial hly gene to serve as a positive control for protection. Mice immune responses to these recombinants, with and without adjuvant CpG, were analyzed, and compared with the recombinants generated with vaccinia strain WR. Co-expression of IL12 and B7 abrogated the protective efficacy of the vaccinia/ pLLO recombinant. / Ph. D.

It is unknown if basic motorcycle training prevents crashes, injuries, or deaths on public roadways. This research analyzes the participants of the Motorcycle Safety Foundation’s Basic RiderCourse updated to understand risk-taking tendencies, personal protective equipment usage, and favorability of the Basic RiderCourse. Data gathered from 168 Basic RiderCourse updated participants in Central Illinois from a pre-test post-test quantitative design using a gain score analysis comprise the methodology. The resulting analysis determined the Basic RiderCourse updated created a positive learning environment and student were more likely to wear personal protective equipment after participating in the course, but the risk-taking behavior and decision making did not change. To further reduce injuries and deaths on public roadways, this research suggests a comprehensive program review of the Basic RiderCourse updated.