Global ETD Search

1	Investigation of the role of the ubiquitin-like DWNN domain in targeting Retinoblastoma Binding Protein 6 to nuclear speckles Mlaza, Mihlali January 2018 (has links) Retinoblastoma Binding Protein 6 (RBBP6) is a 200 KDa protein shown to play a role in 3'- polyadenylation of mRNA transcripts, as well as to function as an E3 ligase catalysing ubiquitination of cancer-associated proteins. RBBP6 has been previously reported to localise to nuclear speckles, which are thought to play a role in mRNA splicing, presumably as a result of its RS domain, which is known to target mRNA splicing factors to nuclear speckles. However recent studies in our laboratory have shown that isoform 3 of RBBP6, consisting mainly of the DWNN domain, also localises to speckles in resting cells, but more strongly in cells subjected to various stresses, suggesting that the DWNN domain may be the speckle-targeting domain. / Magister Scientiae - MSc (Biotechnology) RBBP6 DWNN domain Protein localisation Site-directed mutagenesis Nuclear speckles
2	Syntéza železo-sirných center v Monocercomonoides exilis / Iron-Sulfur cluster assembly in Monocercomonoides exilis Vacek, Vojtěch January 2020 (has links) In the search for the mitochondrion of oxymonads, DNA of Monocercomonoides exilis - an oxymonad isolated from the gut of Chinchilla, was isolated and its genome was sequenced. Sequencing resulted in a fairly complete genome which was extensively searched or genes for mitochondrion related proteins, but no reliable candidate for such gene was identified. Even genes for the ISC pathway, which is responsible for Fe-S cluster assembly and considered to be the only essential function of reduced mitochondrion-like organelles (MROs), were absent. Instead, we were able to detect the presence of a SUF pathway which functionally replaced the ISC pathway. Closer examination of the SUF pathway based on heterologous localisation revealed that this pathway localised in the cytosol. In silico analysis showed that SUF genes are highly conserved at the level of secondary and tertiary structure and most catalytic residues and motifs are present in their sequences. The functionality of these proteins was further indirectly confirmed by complementation experiments in Escherichia coli where SUF proteins of M. exilis were able to restore at least partially Fe-S cluster assembly of strains deficient in the SUF and ISC pathways. We also proved by bacterial adenylate cyclase two-hybrid system that SufB and SufC can form...
3	Regulation der Aktivität und Lokalisation von Antiterminatorproteinen der BglG-Familie / Regulation of the Activity and Localization of BglG family Antiterminator Proteins Rothe, Fabian 23 March 2012 (has links) No description available. 570 Biowissenschaften, Biologie WUE 200 WUK 000 WJL 200 Biology (incl. Psychology) LicT BglG Phosphorylierung Antitermination RNA-Regulation Escherichia coli Bacillus subtilis dynamische Proteinlokalisation Phosphotransferasesystem Genregulation FruB HPr LicT BglG phosphorylation antiterminator RNA regulation Escherichia coli Bacillus subtilis dynamic protein localisation phosphotransferase system gene regulation FruB HPr 42.30 42.13

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