Adolescent alcohol abuse increases across the adolescent years. If left unchecked, alcohol abuse can give rise to delinquency, poor grades, and risky sexual behavior (Stueve & O’Donnell, 2005; Ellickson, Tucker, & Klein, 2003). Past research suggests that minimal parental oversight increases the risk for adolescent alcohol abuse. There is also evidence, however, that parents withdraw from oversight in the face of adolescent problem behaviors (Barber & Olsen, 1997; Hafen & Laursen, 2009). Each may vary according to the child’s physical development. Parents may respond to pubertal maturation with reduced supervision and early maturing girls may be sensitive to parent supervision because of the additional pressures and attention they receive from older, possibly deviant, peers (Stattin, Kerr, & Skoog, 2011). / Includes bibliography. / Thesis (M.A.)--Florida Atlantic University, 2014. / FAU Electronic Theses and Dissertations Collection

O aumento da obesidade infantil tem sido correlacionado com a prevalência da puberdade precoce. Em animais pré-puberes verifica-se que a administração da dieta hiperlipídica induz a antecipação da puberdade. Estudos sugerem que a leptina pode influenciar o eixo reprodutivo por meio de sua ação em neurônios que expressam kisspeptina, no hipotálamo. Uma subpopulação de neurônios kisspeptidérgicos do núcleo arqueado coexpressa a dinorfina e neuroquinina B, que exercem uma ação autorreguladora sobre a secreção de kisspeptina. Considerando que a leptina, a kisspeptina, neuroquinina B e dinorfina são apontados como reguladores do início da puberdade e da função reprodutiva, este trabalho investigou como a dieta hiperlípidica (HFD) pode influenciar esses moduladores ao longo do início do desenvolvimento puberal e após a maturação sexual. Camundongos fêmeas Kiss1-hrGFP foram tratadas com a HFD ou dieta controle a partir do desmame e foram decapitadas aos 28, 32 e 63 dias (Diestro II) e o sangue coletado para dosagem plasmática de leptina, LH e estradiol. Um segundo grupo de fêmeas, tratadas com HFD ou dieta controle, receberam com 32 e 63 dias (Diestro I) uma injeção intraperitoneal de leptina (0,5 mg/kg) ou de veículo, 45 minutos após a injeção foram anestesiadas e submetidas à perfusão cerebral. Cortes hipotalâmicos foram utilizados para realização da técnica de imunoistoquímica para dupla marcação: Kiss1-p-STAT3/ p-STAT3-CART/ Kiss1-Dyn e Kiss1-NKB. Verificamos que a HFD administrada após o desmame, quando comparada à dieta controle, antecipou a abertura vaginal, o primeiro estro e o ínicio da ciclicidade em fêmeas Kiss1-hrGFP. Como indicativo de ativação do eixo reprodutivo neste grupo, foi observado aumento do peso do útero e ovário, aos 32 dias. Esses efeitos estiveram associados com: a) o aumento da concentração plasmática de leptina; b) aumento da ativação de p-STAT3 no núcleo arqueado (ARC) e c) aumento de p-STAT3 em neurônios Kiss1-hrGFP e em neurônios CART do ARC em resposta ao estímulo da leptina. A HFD não afetou a colocalização entre Kiss1-Dyn e Kiss1-NKB em fêmeas no início do desenvolvimento puberal. Em fêmeas Kiss1-hrGFP (63 dias) a administração da HFD por 6 semanas não alterou a regularidade do ciclo estral, houve elevação da leptina plasmática associada com diminuição da expressão da p-STAT3 no ARC em resposta ao estímulo com leptina, sem alterar a responsividade dos neurônios Kiss1-hrGFP a este hormônio. Em conclusão, 9 os resultados demonstram que a antecipação da puberdade induzida pela HFD está associada com o aumento da ação central da leptina, que ao estimular o sistema kisspeptidérgico, direta ou indiretamente, por meio de neurônios CART, pode aumentar o tônus excitatório sobre o eixo reprodutivo. A administração da HFD após o desmame (6 semanas) promoveu uma redução à resposta da leptina no ARC na vida adulta, porém não afetou a responsividade dos neurônios Kiss1-hrGFP a este hormônio e nem a regularidade do ciclo estral nas fêmeas Kiss1-hrGFP. Em conjunto, esses resultados indicam que as ações da leptina nesses neurônios foram preservadas, mesmo com a resistência parcial de suas ações no ARC. Estudos adicionais deverão ser realizados para se avaliar os efeitos a longo prazo da HFD sobre o eixo reprodutivo. / The increase of childhood obesity has been correlated with the early onset of puberty. In prepubertal animals it appears high fat diet (HFD) induces anticipation of puberty. Several studies have indicated that the effect of leptin on reproductive axis can be mediated by its action on Kiss1 neurons in the hypothalamus.A subpopulation of Kiss1 neurons in the arcuate nucleus also shows co-expression of neurokinin B and dynorphin A,presenting a self-regulatory action kisspeptin secretion. Since leptin, kisspeptin, neurokinin B and dynorphin are identified as regulators of puberty onset and reproductive function, this study investigated the influence of high fat diet on these modulators along puberty onset and after sexual maturation. A group of Kiss1-hrGFP-female mice received the HFD or regular chow after weaning. The Kiss1-hrGFP females were decapitated at 28, 32 and 63 days old (Diestrus II) and blood was collected for measurement of plasma leptin, LH and estradiol. A second group of females, treated with HFD or chow after weaning, received at 32 and 63 days old (Diestrus I) an intraperitoneal injection of leptin (0.5 mg / kg) or vehicle, and 45 minutes after they were anesthetized and subjected to cerebral perfusion. Immunohistochemistry was performed for double labeling: Kiss1-p-STAT3/ p-STAT3-CART/ Kiss1-Dyn and Kiss1-NKB in the hypothalamus. It was found that, compared to controls; the HFD anticipated the vaginal opening, the first estrus and the beginning of cyclicity in Kiss1-hrGFP females. An increase in the uterine weight and ovaries, confirmed the activation of reproductive axis in the HFD group at 32 days old. These effects were associated with: a) an increase in plasma leptin concentration; b) the activation of p-STAT3 in the arcuate nucleus (ARC) and c) the increase of p-STAT3 in Kiss1-hrGFP neurons and in CART neurons of the ARC in response to the stimulus of leptin. HFD didnt affect colocalization between Kiss1-Dyn and Kiss1-NKB in females mice at hte pubertal onset . Kiss1-hrGFP female mice (63 days old), exposed to HFD for 6 weeks,exhibited regular estrous cycles, increased plasma levels of leptin associated with a decreased p-STAT3 expression in the ARC after leptin stimulation, with no changes in the responsiveness of Kiss1-hrGFP neurons to this hormone, compared to controls. In conclusion, 11 the results demonstrate that the early puberty induced by the HFD is associated with increased central leptin action, by stimulating the kisspeptidergic system, directly or indirectly, through CART neurons that can increase the excitatory tone in the reproductive axis. The administration of HFD after weaning (6 weeks) induced a decrease on the response to leptin in the ARC in adulthood, but without changes in the responsiveness of Kiss1-hrGFP neurons to this hormone and the regularity of the estrous cycle in Kiss1-hrGFP females. Taken together, these results indicate that the actions of leptin in kisspeptin neurons were preserved after 6 weeks of HFD, despite the partial resistance to this hormone in the ARC. Additional studies should be conducted to evaluate the long-term effects of HFD in the reproductive axis.

A Solanum lycocarpum é uma planta encontrada em quase todos os estados do Brasil, podendo ser ingerida por animais em idade fértil e em condições naturais. Os frutos desta planta possuem glicoalcalóides esteroidais com propriedades semelhantes aos esteróides. Neste estudo investigou-se os efeitos na esfera reprodutiva causados pela ingestão de S. lycocarpum durante a puberdade de ratos. Os frutos secos foram administrados na ração (10%) desde o desmame até a idade adulta (70dias). Foram avaliados o peso corpóreo e de órgãos, o comportamento e a orientação sexual, os níveis de hormônios sexuais nas fezes, a bioquímica sérica, os níveis de neurotransmissores centrais e os achados anatomopatológico, tanto de ratos machos como de fêmeas. Observou-se os seguintes resultados: 1) Redução no peso corpóreo dos machos e das fêmeas; 2) Em machos, o peso relativo aumentou nos epidídimos, vesícula seminal e testículos; 3) Não houve diferenças no hemograma entre os grupos controle e experimental de ambos os sexos; 4) Alterações na bioquímica sangüínea sugerem leve toxicidade em células musculares e hepática; 5) Em fêmeas, não constatou-se nenhuma alteração no ciclo estral e no comportamento sexual; enquanto nos machos houve melhora do comportamento sexual e 6) Os níveis de neurotransmissores do estriado foi modificado mas não os do hipotálamo. Esses achados indicam que a exposição a S. lycocarpum durante a puberdade prejudicou parâmetros da esfera reprodutiva, em particular, dos machos. / Solanum lycocarpum St. Hil, a toxic plant common in almost all states of Brazil, may be consumed by animals in fertile age and in natural condition. The fruits of this plant contain steroidal glycoalkaloids with steroid-like properties. Presently the reproductive effects of S. lycocarpum fruit ingestion during puberty were investigated. The dried fruits were administered in the diet (10%) from the weaning up to adult age (70 days). The body and organs weights, sexual behavior as well as sexual orientation, the feces hormonal levels, hemogram and biochemical blood evaluations, hypothalamic and striatal neurotransmitter levels, and anatomopathological studies of male and female rats were performed. The results showed: 1) a reduced body weight in male and female; 2) in male, an increased epydidimis, seminal vesicle and testis relative weights; 3) no differences of controls and experimental groups of both sex in the hemogram; 4) blood biochemical alterations meaning slight toxicity in muscular and hepatic cells; 5) in female, no interference was detected in estrous cycle and sexual behavior; an improvement of male sexual behavior was observed; 6) that striatal neurotransmitter levels were modified but not in the hypothalamus. These results indicate that exposure to S. lycocarpum during puberty prejudiced the reproductive system, particularly in male rats.

Title: Teaching of orienteering at primary school Author: Bc. Zuzana Procházková Department: Department of Physical Education Supervisor: PaedDr. Ivan Přibyl Objectives: The aim of this study is to present orienteering to pupils of primary school. Examine the effect of orienteering on pupils. Introduce history and the rules of orienteering, the system of competitions and the organization of trainings. Familiarize the reader with the other orienteering sports. Present developmental psychology of children aged 11 to 15. To put orienteering in curriculum for primary school. Methods: The method of questioning was used. We also organized the orienteering race for children. The results were evaluated into spreadsheets, graph and commentary was also used. Results: There are pupils from primary school in this research. Pupils are 11-16 years old. Most of the pupils knew about orienteering before this research. Most pupils ran between 15-20 minutes for one kilometre in the race. Fifteen pupils from 95 didn't find the control point. Pupils felt good after the race. Half from them want to participate orienteering race again. Keywords: Running, map, compass, terrain, orienteering, spare time, puberty

A Solanum lycocarpum é uma planta encontrada em quase todos os estados do Brasil, podendo ser ingerida por animais em idade fértil e em condições naturais. Os frutos desta planta possuem glicoalcalóides esteroidais com propriedades semelhantes aos esteróides. Neste estudo investigou-se os efeitos na esfera reprodutiva causados pela ingestão de S. lycocarpum durante a puberdade de ratos. Os frutos secos foram administrados na ração (10%) desde o desmame até a idade adulta (70dias). Foram avaliados o peso corpóreo e de órgãos, o comportamento e a orientação sexual, os níveis de hormônios sexuais nas fezes, a bioquímica sérica, os níveis de neurotransmissores centrais e os achados anatomopatológico, tanto de ratos machos como de fêmeas. Observou-se os seguintes resultados: 1) Redução no peso corpóreo dos machos e das fêmeas; 2) Em machos, o peso relativo aumentou nos epidídimos, vesícula seminal e testículos; 3) Não houve diferenças no hemograma entre os grupos controle e experimental de ambos os sexos; 4) Alterações na bioquímica sangüínea sugerem leve toxicidade em células musculares e hepática; 5) Em fêmeas, não constatou-se nenhuma alteração no ciclo estral e no comportamento sexual; enquanto nos machos houve melhora do comportamento sexual e 6) Os níveis de neurotransmissores do estriado foi modificado mas não os do hipotálamo. Esses achados indicam que a exposição a S. lycocarpum durante a puberdade prejudicou parâmetros da esfera reprodutiva, em particular, dos machos. / Solanum lycocarpum St. Hil, a toxic plant common in almost all states of Brazil, may be consumed by animals in fertile age and in natural condition. The fruits of this plant contain steroidal glycoalkaloids with steroid-like properties. Presently the reproductive effects of S. lycocarpum fruit ingestion during puberty were investigated. The dried fruits were administered in the diet (10%) from the weaning up to adult age (70 days). The body and organs weights, sexual behavior as well as sexual orientation, the feces hormonal levels, hemogram and biochemical blood evaluations, hypothalamic and striatal neurotransmitter levels, and anatomopathological studies of male and female rats were performed. The results showed: 1) a reduced body weight in male and female; 2) in male, an increased epydidimis, seminal vesicle and testis relative weights; 3) no differences of controls and experimental groups of both sex in the hemogram; 4) blood biochemical alterations meaning slight toxicity in muscular and hepatic cells; 5) in female, no interference was detected in estrous cycle and sexual behavior; an improvement of male sexual behavior was observed; 6) that striatal neurotransmitter levels were modified but not in the hypothalamus. These results indicate that exposure to S. lycocarpum during puberty prejudiced the reproductive system, particularly in male rats.

Introduction Epidemiologic data supporting the role of organochlorine pesticides in pubertal development are limited. Methods Using a nested case-control design, serum collected during pregnancy from mothers of 218 girls who reported menarche before 11.5 years of age (cases) and 230 girls who reported menarche at or after 11.5 years of age (controls) was analyzed for 9 organochlorines and metabolites. We analyzed the association between in utero organochlorine concentrations and early menarche using multivariate logistic regression controlling for mother's age at menarche, or mother's prenatal BMI. Results We did not observe an association between in utero exposure to HCB, β-HCH, ϒ-HCH, p,p′-DDT, p,p′-DDE, oxychlordane or trans-nonachlor and early menarche. Conclusions This study is the first to examine the association between in utero exposure to HCB, β-HCH, ϒ-HCH, oxychlordane or trans-nonachlor and early menarche. In utero exposure to organochlorine pesticides does not appear to have a role in the timing of menarche in this study.

Previous research suggests that as well as behavioural difficulties, a small subset of aggressive and antisocial children show callous unemotional (CU) personality traits (i.e., lack of remorse and absence of empathy) that set them apart from their low-CU peers. These children have been identified as being most at risk to follow a path of severe and persistent antisocial behaviour, showing distinct behavioural patterns, and have been found to respond less to traditional treatment programs. One particular focus of this thesis is that emerging findings have shown emotion recognition deficits within both groups. Whereas children who only show behavioural difficulties (in the absence of CU traits) have been found to misclassify vague and neutral expressions as anger, the presence of CU traits has been associated with an inability to correctly identify fear and to a lesser extend, sadness. Furthermore, emotion recognition competence varies with age and development. In general, emotion recognition improves with age, but interestingly there is some evidence that it may become less efficient during puberty. No research could be located, however, that assessed emotion recognition through childhood and adolescence for children high and low on CU traits and antisocial behaviour. The primary focus of this study was to investigate the impact of these personality traits and pubertal development on emotion recognition competence in isolation and in combination. A specific aim was to assess if puberty would exacerbate these deficits in children with pre-existing deficits in emotion recognition. The effect of gender, emotion type and measure characteristics, in particular the age of the target face, was also examined. A community sample of 703 children and adolescents aged 7-17 were administered the Strength and Difficulties Questionnaire to assess adjustment, the Antisocial Process Screening Device to assess antisocial traits, and the Pubertal Development Scale was administered to evaluate pubertal stage. Empathy was assessed using the Bryant Index of Empathy for Children and Adolescents. Parents or caregivers completed parent version of these measures for their children. Emotion recognition ability was measured using the newly developed UNSW FACES task (Dadds, Hawes & Merz, 2004). Description of the development and validation of this measure are included. Contrary to expectations, emotion recognition accuracy was not negatively affected by puberty. In addition, no overall differences in emotion recognition ability were found due to participant’s gender or target face age group characteristics. The hypothesis that participants would be better at recognising emotions expressed by their own age group was therefore not supported. In line with expectations, significant negative associations between CU traits and fear recognition were found. However, these were small, and contrary to expectations, were found for girls rather than boys. Also, puberty did not exacerbate emotion recognition deficits in high CU children. However, the relationship between CU traits and emotion recognition was affected differently by pubertal status. The implications of these results are discussed in relation to future research into emotion recognition deficits within this population. In addition, theoretical and practical implications of these findings for the development of antisocial behaviour and the treatment of children showing CU traits are explored.

The follicle and its enclosed oocyte share intimate and critical communication that regulates folliculogenesis and produces a mature oocyte. Protein and RNA accumulated in the oocyte during oogenesis control fertilization and direct embryonic development until the embryonic genome activates. Most knowledge of mammalian oocyte biology has been derived from eutherian species. Marsupials deserve more detailed studies because they have a distinct reproductive biology that offers a unique perspective from which to consider mammalian reproduction. The oocyte biology of the tammar wallaby, Macropus eugenii, is the focus of research in this thesis. Cold storage, a simple method for transporting ovarian tissue, was evaluated using histological techniques and follicle culture to assess the structure and function of tammar ovarian tissue. In vitro techniques were used to examine and compare: -folliculogenesis in prepubertal and adult animals, - fertilization of in vivo and in vitro matured oocytes, - and embryo development in follicular and tubal oocytes. / Tammar ovaries were placed in cold storage (PBS at 4?C) for 24 or 48 hours. Necrotic changes were minimal in ovarian follicles after cold storage and preantral follicles isolated from ovarian tissue after cold storage grew by similar amounts as non-stored follicles when cultured for 4 days in vitro. Although the general morphology and growth of follicles are unaffected after cold storage for up to 48 hours, the viability of the oocyte is of prime importance. The next important stages of this study were to develop in vitro techniques for follicle culture and for oocyte maturation and fertilization for future assessment of oocytes after cold storage. / A defined (serum-free) culture system was developed to grow isolated preantral follicles from prepubertal and adult tammars. FSH promoted follicle growth and antrum formation in follicles from prepubertal tammars. Although FSH promoted growth in follicles from adult tammars, other factors present in serum were required for antrum formation. Therefore, once the hypothalmo-pituitary-gonadal axis is mature, hormones and growth factors modify the mechanism of antrum formation. Only follicles that developed an antrum in the presence of serum had granulosa and theca layers that had appropriately differentiated. While FSH stimulates follicle growth in vitro, more complex conditions are required to promote granulosa and theca differentiation. / Intra-cytoplasmic sperm injection (ICSI) was successfully used to compare fertilization of in vivo and in vitro matured oocytes as well as the development of mature oocytes collected from the ovary (surrounded by zona pellucida) or from the oviduct (surrounded by zona pellucida and mucoid coat). In vitro matured oocytes proceeded though the early stages of fertilization (e.g. sperm nuclear decondensation, pronuclear formation), but not syngamy. After sperm injection, in vivo matured oocytes cleaved as far as the 8-cell stage. Oocytes do not lose their ability to fertilize after acquisition of the mucoid coat, since tubal oocytes cleaved as far as the 8-cell stage after sperm injection. Follicular oocytes develop as far as the 5-cell stage after sperm injection, but embryos had a large cleavage cavity that hindered cell-cell contact. While the mucoid coat is not required for cleavage, it is important for appropriate cell-cell interaction and normal early development of the embryo. / This, the most detailed in vitro study of marsupial oocyte biology, has shown that there are many similarities in the biology of marsupial and eutherian oocytes but that the unique biology of marsupials offers a significant perspective on mammalian reproduction. This work also lays the foundation for the effective use of assisted reproductive techniques for conservation of Australia’s unique mammalian fauna.

<p>The study is about the rock engravings on the wonderstone hills just outside Ottosdal, North West province, about 70km northwest of Klerksdorp. Wonderstone is remarkable rock that is&nbsp / smooth, shiny and very easy to mark. The wonderstone occurs only on two adjacent farms, Gestoptefontein and Driekuil, and thus the rock art on the wonderstone outcrops is referred to as the Gestoptefontein- Driekuil complex (GDC). This rock art is now the only remaining trace of what must once have been a much larger complex of engravings. Sadly, much of the rock art has been destroyed in the course of mining activities, with very few records. The largest remaining outcrop is still threatened by potential mining activities. The study attempts to bring this disastrous and unacceptable situation to the attention of the public and the heritage authorities, who have so far failed to respond to applications to grant the sites protection. It therefore has two main aims: to&nbsp / locate and record as much of the rock art as possible and to understand the significance of the outcrops in the lives of the people who made them. Based on the rock art itself, as well as what little historical evidence is available, it is argued that the rock art was made by Khoe-San people during the performance of important ceremonies and other activities. The rock art has two main components: engravings of referential motifs and a gestural, or performative, element. The referential motifs depict a range of things: anthropomorphs and zoomorphs, decorative designs, items of clothing, as well as ornaments and decorations. The gestural markings were made by rubbing, cutting and hammering the soft wonderstone, probably in the course of a range of activities that people carried out on the outcrops. One of the main findings of the study is that the GDC was a place that was of particular significance to women. This is suggested by the large number of engravings of items that are closely associated with Khoe-San women &ndash / depictions of aprons, ornaments, and decorations. These play a prominent role in the initiation practices of many Khoe-San groups. Initiates emerging from ritual isolation after their first menstruation are given new clothes / they are also loaned ornaments and jewellery. This reincorporation into society as a &lsquo / new person&rsquo / has been described by some Khoe-San women as one of the high points of their lives. Oral traditions from the area indicate that the wonderstone outcrops were believed to have&nbsp / special properties / the study incorporates these traditions to argue that the wonderstone outcrops were associated with the presence of a great water snake that lay on the rocks and also lived in&nbsp / the pools of water in the nearby Driekuil Spruit. People therefore came to the outcrops to perform rites of reincorporation. One of these ceremonies may have been performing rites of association&nbsp / with the great snake / such practices may have included the use of rock dust as an ingredient for body paint.</p>

<p>The study is about the rock engravings on the wonderstone hills just outside Ottosdal, North West province, about 70km northwest of Klerksdorp. Wonderstone is remarkable rock that is&nbsp / smooth, shiny and very easy to mark. The wonderstone occurs only on two adjacent farms, Gestoptefontein and Driekuil, and thus the rock art on the wonderstone outcrops is referred to as the Gestoptefontein- Driekuil complex (GDC). This rock art is now the only remaining trace of what must once have been a much larger complex of engravings. Sadly, much of the rock art has been destroyed in the course of mining activities, with very few records. The largest remaining outcrop is still threatened by potential mining activities. The study attempts to bring this disastrous and unacceptable situation to the attention of the public and the heritage authorities, who have so far failed to respond to applications to grant the sites protection. It therefore has two main aims: to&nbsp / locate and record as much of the rock art as possible and to understand the significance of the outcrops in the lives of the people who made them. Based on the rock art itself, as well as what little historical evidence is available, it is argued that the rock art was made by Khoe-San people during the performance of important ceremonies and other activities. The rock art has two main components: engravings of referential motifs and a gestural, or performative, element. The referential motifs depict a range of things: anthropomorphs and zoomorphs, decorative designs, items of clothing, as well as ornaments and decorations. The gestural markings were made by rubbing, cutting and hammering the soft wonderstone, probably in the course of a range of activities that people carried out on the outcrops. One of the main findings of the study is that the GDC was a place that was of particular significance to women. This is suggested by the large number of engravings of items that are closely associated with Khoe-San women &ndash / depictions of aprons, ornaments, and decorations. These play a prominent role in the initiation practices of many Khoe-San groups. Initiates emerging from ritual isolation after their first menstruation are given new clothes / they are also loaned ornaments and jewellery. This reincorporation into society as a &lsquo / new person&rsquo / has been described by some Khoe-San women as one of the high points of their lives. Oral traditions from the area indicate that the wonderstone outcrops were believed to have&nbsp / special properties / the study incorporates these traditions to argue that the wonderstone outcrops were associated with the presence of a great water snake that lay on the rocks and also lived in&nbsp / the pools of water in the nearby Driekuil Spruit. People therefore came to the outcrops to perform rites of reincorporation. One of these ceremonies may have been performing rites of association&nbsp / with the great snake / such practices may have included the use of rock dust as an ingredient for body paint.</p>