Thesis, PlanB (M.S.)--University of Wisconsin--Stout, 2005. / Includes bibliographical references.

Thesis (M.S.)--University of Louisville, 2002. / Department of Industrial Engineering. Vita. "December 2002." Includes bibliographical references (leaves 62-63).

Thesis (Ph. D.)--Ohio State University, 2005. Thesis (Ph. D. in Philosophy)--The Ohio State University, 2005. / Microfilm copy: University Microfilms No. 3161111. Includes bibliographical references (p. 143-162). Also available online online via OhioLINK's ETD Center

Thesis (M.Sc.)(Chemistry)--University of Pretoria, 2006. / Summaries in English and Afrikaans. Includes bibliographical references. Available on the Internet via the World Wide Web.

Ph.D.

Orientador: Regina Kiomi Takahira / Banca: Raimundo Souza Lopes / Banca: Patrícia Mendes Pereira / Resumo: O concentrado de plaquetas é um hemocomponente importante para a transfusão de grandes quantidades de plaquetas. Durante a estocagem, as plaquetas sofrem lesões que devem ser analisadas por meio de testes laboratoriais. O objetivo do presente estudo foi avaliar as qualidades físicas, químicas, microbiológicas, estruturais e funcionais plaquetárias em concentrados de plaquetas caninos estocados durante 5 e 7 dias. Foram realizadas contagem de plaquetas, leucócitos e hemácias por unidade, mensuração do pH, glicose, avaliação do swirling, análise microbiológica, avaliação da agregação plaquetária e da expressão da GPIIbIIIa pela citometria de fluxo. As análises foram realizadas logo após a centrifugação, e no quinto (grupo I) e sétimo (grupo II) dias de estocagem dos concentrados de plaquetas obtidos de 22 cães hígidos. Todas as bolsas foram negativas para o isolamento de microorganismos. Houve diminuição significativa nas contagens de plaquetas e leucócitos, pH, glicose e swirling nos dois grupos. Ocorreu diminuição na resposta agregatória nos dois grupos, com o uso isolado e associado de ADP e epinefrina. Os anticorpos CD41 e CD61 apresentaram alta porcentagem de fluorescência em todos os momentos, e não houve variação significativa na média de intensidade de fluorescência das plaquetas durante a estocagem. Conclui-se que, mantida a esterilidade microbiológica, os concentrados de plaquetas caninas podem ser estocados por até 7 dias, no entanto, há uma maior perda da viabilidade plaquetária e qualidade dos concentrados quando comparados ao grupo armazenado durante 5 dias. Houve perda funcional das plaquetas durante a estocagem pela redução da agregação plaquetária. Os anticorpos CD41 e CD61 podem ser utilizados na avaliação da GPIIbIIIa de plaquetas caninas estocadas durante os períodos avaliados / Abstract: Platelet concentrate is an important blood component for platelet transfusion. During storage, platelets undergo lesions that should be evaluated by laboratory assays. The aim of this study was to evaluate the physical, chemical, microbiological, structural and functional platelet qualities in canine platelet concentrate stored for 5 and 7 days. Platelet, leukocytes and erythrocytes concentrations per unit, pH and glucose measurements, swirling observation, microbiological analysis, assessment of platelet agregation and expression of GPIIbIIIa by flow cytometry were performed. Analyses were performed after centrifugation, and the fifth (group I) and seventh (group II) days of platelet concentrate storage obtained from 22 healthy dogs. All platelet concentrate bags were negative for microorganisms isolation. Platelet and leukocytes concentrations, pH, glucose, and swirling decreased significantly during storage, in both groups. A significant reduction in aggregatory response was detected in both groups, in the presence of ADP, epinephrine or both simultaneously. CD41 and CD61 antibodies showed high fluorescence at all times, and no significant variation in mean fluorescence intensity during storage. In conclusion, canine platelet concentrates should be stored for 5 days, however, can be stored for up to 7 days with greater loss of viability and quality of bags during this period. There was functional loss observed by the reduction of platelet aggregation during storage in both groups. CD41 and CD61 antibodies can be used to assess GPIIbIIIa in canine platelets stored during the evaluated period / Mestre

Made available in DSpace on 2014-06-11T19:27:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-03-17Bitstream added on 2014-06-13T20:17:37Z : No. of bitstreams: 1 costa_ccmr_me_botfmvz.pdf: 292935 bytes, checksum: 632cc3655cad59e9e602b2040d94e522 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O concentrado de plaquetas é um hemocomponente importante para a transfusão de grandes quantidades de plaquetas. Durante a estocagem, as plaquetas sofrem lesões que devem ser analisadas por meio de testes laboratoriais. O objetivo do presente estudo foi avaliar as qualidades físicas, químicas, microbiológicas, estruturais e funcionais plaquetárias em concentrados de plaquetas caninos estocados durante 5 e 7 dias. Foram realizadas contagem de plaquetas, leucócitos e hemácias por unidade, mensuração do pH, glicose, avaliação do swirling, análise microbiológica, avaliação da agregação plaquetária e da expressão da GPIIbIIIa pela citometria de fluxo. As análises foram realizadas logo após a centrifugação, e no quinto (grupo I) e sétimo (grupo II) dias de estocagem dos concentrados de plaquetas obtidos de 22 cães hígidos. Todas as bolsas foram negativas para o isolamento de microorganismos. Houve diminuição significativa nas contagens de plaquetas e leucócitos, pH, glicose e swirling nos dois grupos. Ocorreu diminuição na resposta agregatória nos dois grupos, com o uso isolado e associado de ADP e epinefrina. Os anticorpos CD41 e CD61 apresentaram alta porcentagem de fluorescência em todos os momentos, e não houve variação significativa na média de intensidade de fluorescência das plaquetas durante a estocagem. Conclui-se que, mantida a esterilidade microbiológica, os concentrados de plaquetas caninas podem ser estocados por até 7 dias, no entanto, há uma maior perda da viabilidade plaquetária e qualidade dos concentrados quando comparados ao grupo armazenado durante 5 dias. Houve perda funcional das plaquetas durante a estocagem pela redução da agregação plaquetária. Os anticorpos CD41 e CD61 podem ser utilizados na avaliação da GPIIbIIIa de plaquetas caninas estocadas durante os períodos avaliados / Platelet concentrate is an important blood component for platelet transfusion. During storage, platelets undergo lesions that should be evaluated by laboratory assays. The aim of this study was to evaluate the physical, chemical, microbiological, structural and functional platelet qualities in canine platelet concentrate stored for 5 and 7 days. Platelet, leukocytes and erythrocytes concentrations per unit, pH and glucose measurements, swirling observation, microbiological analysis, assessment of platelet agregation and expression of GPIIbIIIa by flow cytometry were performed. Analyses were performed after centrifugation, and the fifth (group I) and seventh (group II) days of platelet concentrate storage obtained from 22 healthy dogs. All platelet concentrate bags were negative for microorganisms isolation. Platelet and leukocytes concentrations, pH, glucose, and swirling decreased significantly during storage, in both groups. A significant reduction in aggregatory response was detected in both groups, in the presence of ADP, epinephrine or both simultaneously. CD41 and CD61 antibodies showed high fluorescence at all times, and no significant variation in mean fluorescence intensity during storage. In conclusion, canine platelet concentrates should be stored for 5 days, however, can be stored for up to 7 days with greater loss of viability and quality of bags during this period. There was functional loss observed by the reduction of platelet aggregation during storage in both groups. CD41 and CD61 antibodies can be used to assess GPIIbIIIa in canine platelets stored during the evaluated period

Made available in DSpace on 2014-10-09T12:36:40Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T13:59:09Z (GMT). No. of bitstreams: 1 04230.pdf: 1353263 bytes, checksum: ba0b1a87d30ef196c0398dd1d7b4ca6c (MD5) / Dissertacao (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP

Made available in DSpace on 2014-10-09T12:27:37Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:04:29Z (GMT). No. of bitstreams: 0 / Dissertacao (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP

Dissertation submitted in fulfillment of the requirements for the degree Magister Technologiae: Quality in the Faculty of Engineering at the Cape Peninsula University of Technology / The Quality Control laboratory in the South African Breweries (SAB) measures the quality of raw materials to final product to enable the plant to make process changes if required. The brewing laboratory also co-ordinates taste sessions, and maintains and calibrates equipment for the plant. They also have internal verification (Reference control) systems and validation (standards) systems in place to ensure accuracy of measurements. The Service Level Agreement (SLA) scores according to the researcher are low owing to staff cuts and ineffective time management.The aim of this research is to improve SLA scores and to investigate if using SLA scores is the best way to measure service quality in the Quality Control department of SAB. The researcher aims to use SERVQUAL as a measuring tool to measure service quality in conjunction with SLA‟s. Because the laboratory is a service department, it is expected by brewing internal customers that the laboratory provides the plant with quality data and equipment support in the virtual laboratories in the plant. Furthermore it is expected that the service provided will always be of a high standard, and that SLA scores will be of a high standard as well.The major issue with service quality in the SAB laboratory is the low level of SLA scores. The focus areas of SLA‟s currently are:  Quality Assurance (QA)/ Technical/ Production interaction.  Reference control.  Equipment support.  Routine analysis and ad hoc requests.  Trial support.  In process taste requirements.  Package product taste requirements (Routine, profile, trials and flavour stability). The low score of SLA‟S are mainly due to equipment support and reference control. The other factors also contribute to the low score; however the above mentioned two consistently return low scores. The results of service quality are not significantly improved through the use of SLA‟s calling for the requirement to improve service quality using the SERVQUAL instrument.