Orientador: Carlos Henrique Inacio Ramos / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-26T03:59:04Z (GMT). No. of bitstreams: 1 Peres_BarbaraRamalho_M.pdf: 4607988 bytes, checksum: e396f207db591110a7882ea1df2cfbd4 (MD5) Previous issue date: 2014 / Resumo: As proteínas Rvb1 e Rvb2 são DNA helicases dependentes de ATP pertencentes à família AAA+ ATPases (ATPases associadas com diversos processos celulares) que estão envolvidas na remodelação da cromatina, montagem da telomerase, mitose e biogênese de snoRNP ("small nucleolar ribonucleoprotein") e interagem com as co-chaperonas Tah1 (contém um domínio TPR associado com Hsp90) e Pih1 formando um complexo funcional, denominado R2TP. Este complexo já foi identificado em levedura e seres humanos, e desempenha um papel essencial na montagem de complexos multi-proteicos com DNA e RNA. Nosso grupo de pesquisa tem por objetivo identificar e caracterizar o complexo R2TP em Leishmania major pela importância deste parasita para medicina. Apresentamos o estudo das proteínas Rvb1 e Rvb2 de L. major. Os genes foram clonados e as proteínas recombinantes foram purificadas e caracterizadas quanto à conformação pelas técnicas de dicroísmo circular, gel filtração analítica e fluorescência intrínseca, mostrando que as proteínas foram purificadas enoveladas. Foram feitos ensaios funcionais de atividade ATPásica mostrando que estas possuem atividade ATPásica a qual é aumentada quando as proteínas foram incubadas com DNA. Espera-se que os resultados deste trabalho possam contribuir para o objetivo de compreensão do sistema R2TP e da regulação da homeostase do genoma de L. major. O conjunto destas pesquisas pode contribuir também para o desenvolvimento de possíveis estratégias para intervir no parasita causador de uma doença negligenciada / Abstract: Rvb1 and Rvb2 proteins are ATP-dependent DNA helicases belonging to the AAA + ATPases family (ATPases associated with diverse cellular processes) that are involved in chromatin remodeling, telomerase assembly, mitosis and snoRNP biogenesis ("small nucleolar ribonucleoprotein") and interact with co-chaperones Tah1 (wich contains a TPR domain associated with Hsp90) and Pih1 into a functional complex, named R2TP. The R2TP complex has been identified in yeast and humans, and has an essential role in the assembly of multi-protein complexes with DNA and RNA. Due to the medical importance of Leishmania major our research group has becoming interested in the identification and characterization of the R2TP complex of this parasite. We studied the Rvb1 and Rvb2 proteins of L. major. The genes were cloned, the recombinant proteins were purified and their conformation were characterized by circular dichroism, analytical gel filtration and intrinsic fluorescence techniques, indicating that the proteins were folded. Functional assays showed that Rvb1 and Rvb2 have ATPase activity and this activity increased when incubated with DNA. Hopefully, the results of this study may contribute to the goal of understanding the R2TP system and homeostasis of the L. major genome and together, may contribute to the development of possible strategies to intervene with a parasite that causes a neglected disease / Mestrado / Bioquimica / Mestra em Biologia Funcional e Molecular

Les protéines de la famille L7Ae sont les constituants de nombreuses RNP essentielles. Chez les vertébrés, les particules snoRNP C/D et H/ACA sont impliquées dans la biogenèse des ribosomes, la UsnRNP U4 dans l'épissage des pré-ARNm, le complexe télomérase dans la réplication des télomères, et les mRNP SECIS dans la traduction des sélénoprotéines. Comme c'est le cas pour la majorité des RNP eucaryotes, leur assemblage, sous forme d'entités fonctionnelles, ne constitue pas un processus autonome et requiert l'intervention de facteurs spécialisés. En basant notre étude sur l'assemblage des snoRNP C/D, dans l'organisme modèle Saccharomyces cerevisiae, et en utilisant des approches de biologie moléculaire, de biochimie et de génétique, nous avons entrepris de caractériser ces événements. Nos travaux ont contribué à identifier un ensemble de protéines, agissant de façon coordonnée au sein d'une machinerie conservée entre la levure et l'homme. Cette dernière est composée de deux principales sous-unités : (i) Rsa1p/NUFIP, une protéine plate-forme, qui interagit avec certaines protéines de la famille L7Ae et facilite l'assemblage des RNP, (ii) le complexe R2TP (Rvb1p/TIP49, Rvb2p/TIP48, Pih1p/PIH1, Tah1p/SPAGH), qui pourrait opérer des remodelages conformationnels nécessaires à la formation des RNP matures. En plus de ces acteurs centraux, d'autres facteurs sont apparus intimement liés à ce mécanisme. La protéine Hit1p/TRIP3, interagit notamment avec Rsa1p/NUFIP et s'est avéré requise pour assurer sa stabilité chez la levure. La chaperonne HSP90, dont le rôle est prédominant chez l'homme, exerce son activité sur certains constituants des RNP. Enfin, la protéine Bcd1p/BCD1 pourrait être associée à cette machinerie dans le cadre spécifique de l'assemblage des snoRNP C/D / The L7Ae family proteins are essential components of many RNPs. In vertebrates, C/D and H/ACA snoRNPs are involved in ribosome biogenesis, the U4 snRNP in pre-mRNA splicing, the telomerase complex in telomeres replication, and mRNP SECIS in selenoproteins translation. Like most eukaryotic RNPs, assembly in functional entities is not an autonomous process and requires the intervention of specialized factors. Basing our study on the assembly of C/D snoRNP in the model organism Saccharomyces cerevisiae, and using approaches of molecular biology, biochemistry and genetics, we undertook to decipher these mechanisms. Our work has helped to identify a set of proteins, acting in a coordinated manner within a machinery conserved between yeast and human. This machinery consists of two major subunits: (i) Rsa1p/NUFIP, a platform protein that interacts with some proteins of the L7Ae family and facilitates the RNPs assembly, (ii) the R2TP complex (Rvb1p/TIP49, Rvb2p/TIP48, Pih1p/PIH1, Tah1p/SPAGH), which could induce conformational remodeling necessary for the formation of mature RNPs. In addition to these key players, other factors appeared closely linked to this mechanism. The Hit1p/TRIP3 protein interacts with Rsa1p/NUFIP and is required to ensure its stability in yeast. HSP90 chaperone, whose role is predominant in human, operates on some components of the RNPs. Finally, the Bcd1p/BCD1 protein is associated specifically with this machinery during C/D snoRNPs assembly