Global ETD Search

11	Identifikace nového mechanismu regulace Lck zprostředkovanou její C-terminální sekvencí / Identification of a new mechanism of Lck regulation via its C-terminal sequence Valečka, Jan January 2014 (has links) T-cell activation is a complex process crucial for a proper function of immune system. It has been extensively studied and its main features are well understood. However, some of the events involved in T-cell signalling are still unclear. After T-cell receptor stimulation, Src-family kinase Lck drives the initiation of signalling by tyrosine phosphorylation. Phosphorylation of several downstream targets is dependent on the redistribution of Lck to the different compartment of the plasma membrane, called lipid rafts. In lipid rafts, active Lck is juxtaposed and activates raft-resident substrates which then trigger downstream signalling. The critical in this process is the mechanism of Lck translocation to lipid rafts which has not been studied so far and represents the topic of great academic and clinical interests. Previously, we identified the adaptor protein RACK1 as a candidate protein mediating the redistribution of Lck to lipid rafts by linking it to the microtubular network. In this thesis, we analysed the structural features and functional role of RACK1 in its interaction with Lck. We show here, using the SYF cell lines expressing the wild type and various mutated forms of Lck, that intact SH3 or SH2 domains of Lck are required for an effective RACK1-Lck complex formation. We also documented...
12	Mapování kontaktních míst mezi eukaryotickým translačním iniciačním faktorem eIF3 a 40S ribozomální podjednotkou. / Mapping the contact points between eukaryotic translation initiation factor eIF3 and the 40S ribosomal subunit. Kouba, Tomáš January 2013 (has links) Translation initiation in eukaryotes is a multistep process requiring the orchestrated interaction of several eukaryotic initiation factors (eIFs) together with the small ribosomal subunit to locate the mRNA's translational start and to properly decode the genetic message that it carries. The largest of these factors, eIF3, forms the scaffold for other initiation factors to promote their spatially coordinated placement on the ribosomal surface. It is our long-standing pursuit to map the 40S-binding site of the yeast multisubunit eIF3 and here we present three new mutual interactions between these two macromolecules (i) The C-terminal region of the eIF3c/NIP1 subunit is comprised of the conserved bipartite PCI domain and we show that a short C-terminal truncation and two clustered mutations directly disturbing the PCI domain produce lethal or slow growth phenotypes and significantly reduce amounts of 40S-bound eIF3 in vivo. The extreme C-terminus directly interacts with small subunit ribosomal protein RACK1/ASC1, which is a part of the 40S head, and, consistently, deletion of ASC1 impairs eIF3 association with ribosomes. The PCI domain per se shows strong but unspecific binding to RNA, for the first time implicating this protein fold in protein-RNA interactions. We conclude that the c/NIP1...
13	RACK1 regulates point contact formation and local translation in neuronal growth cones Kershner, Leah 23 April 2018 (has links) No description available. Biology Cellular Biology Molecular Biology Neurosciences Developmental Biology growth cone local translation axon guidance RACK1 point contact neural development
14	Regulace signalní dráhy ERK prostřednictvím scaffold proteinu RACK1 / The regulation of the ERK signalling pathway by scaffold protein RACK1 Bráborec, Vojtěch January 2012 (has links) The ERK signalling cascade comprised of protein kinases Raf, MEK and ERK is an evolutionarily conserved member of MAPK family that is activated in response to wide range of extracellular stimuli. The ERK pathway controls fundamental cellular functions including cell proliferation, differentiation, apoptosis or cell motility. To control such a diverse cellular responses by a single pathway cells have evolved regulatory mechanisms that channel the extracellular signals towards the specific biological response. Crucial to this control are non- enzymatic proteins termed scaffolds that associate with and enhance functional interaction of the components of MAPK pathways and can regulate amplitude, timing, specificity and location of signals. Scaffold protein RACK1 associates with several components of cell migration machinery including integrins, FAK, Src and the ERK pathway core protein kinases. RACK1 regulates distinct steps of cell migration such as establishment of cell polarity and focal adhesion turnover, however, the molecular mechanism by which RACK1 regulates these processes remains largely unknown. The main aim of this study was to investigate the functional role of RACK1 in cell motility, in particular to identify new effector proteins utilized by the ERK pathway and RACK1 in the regulation of...
15	Translational control by the ribosomal protein Asc1p/Cpc2p in Saccharomyces cerevisiae / Translationelle Kontrolle durch das ribosomale Protein Asc1p/Cpc2p in Saccharomyces cerevisiae Rachfall, Nicole 27 October 2010 (has links) No description available. 570 Biowissenschaften, Biologie Biology (incl. Psychology) translationelle Regulation 5'UTR Signaltransduktion Proteomik Microarray Zellmetabolismus Saccharomyces cerevisiae Bäckerhefe Asc1 Cpc2 RACK1 Ribosom metabolische Markierung 2D PAGE translational regulation 5'UTR signal transduction proteomics microarray cell metabolism Saccharomyces cerevisiae baker's yeast Asc1 Cpc2 RACK1 ribosome metabolic labeling 2D PAGE 42.13 42.30 WU 000: Mikrobiologie

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