<p>The thesis is focused on lubricants and roll wear in striprolling.</p><p>Regarding lubricants, the possibility of introducing newones, which are less detrimental towards environment, isstudied in laboratory scale. This is done for cold rolling,both of an Al- alloy and a low carbon steel. The lubricationperformance of such newly developed–water-based–synthetic lubricants is compared with currently used mineraloils and emulsions. By using the experimental method of"forward slip" measurements combined with slab methodcalculations and FE- simulations, friction coefficients areevaluated for different single pass reductions. Lubricantsbearing capacity and the product surface roughness are alsoevaluated. The results are encouraging.</p><p>A synthetic water-based lubricant, used in the cold rollingof an Al- alloy, showed good lubrication capability, betterthan the mineral oil but worse than the emulsion. The rolledAl- strip finish was found to be finest for the syntheticlubricant followed by the mineral oil and the emulsion. Similarresults were obtained from the steel rolling. Here foursynthetic lubricants were compared with two mineral oils andone emulsion. The best lubricant was found to be one of thewater-based synthetics, showing the lowest value of thefriction coefficient and a smooth product surface.</p><p>The aim of the wear study is to develop an accurateroll-wear prediction for hot strip finishing mills, which takesmore influential parameters into account. A new model of higheraccuracy is presented. This model is based on a large amount ofproduction campaigns. The strategy of the work is describedbelow.</p><p>After a comprehensive literature study a promising modelstructure was found. The corresponding equation is tested ontwo hot strip mills. Predicted wear is found to be inqualitative agreement with industrial experience and measuredwear. Thus the structure–taking the influence of back-uprolls into account–was chosen for further development.This was done on behalf of results obtained from one mill builtup by six stands and three different work-roll materials.Campaigns of "mixed" and "similar" strip grades were used. Theobtained model is tested successfully in two other hot stripmills.</p><p>Contradictory to the currently used on-line model, the newmodel takes the work roll flattening and back-up rolls contactarea into consideration. Also the influence of strip- and workroll grades is included. The model enables increased rolledstrip length and prolonged lifetime of the rolls, because ofimproved process control. Further more, grinding cost and timefor roll changing can be minimized. Of course thesepossibilities should result in considerable energy saving.</p><p><b>Keywords:</b>Strip rolling, lubricants, environment, rollwear modeling, production campaigns, regression analysis</p>

It is the aim of this thesis to trace the short history of rock journalism, to examine the factors that led to its development, and to evaluate Rolling stone, the most successful rock magazine and the only one to attract a general-interest audience. Emphasis was placed on the factual development of the publications. Personalities were not developed and a statistical content analysis was avoided in favor of an interpretation of trends. Rock journalism has not yet received an objective evaluation. Its treatment in the several books on the underground press has been superficial and couched in political or even moral terms. By every indication, the rock magazines have exerted more influence than have the underground newspapers and should be accorded proper study. / text

Video data has increased dramatically in recent years due to the prevalence of handheld cameras. Such videos, however, are usually shakier compared to videos shot by tripod-mounted cameras or cameras with mechanical stabilizers. In addition, most handheld cameras use CMOS sensors. In a CMOS sensor camera, different rows in a frame are read/reset sequentially from top to bottom. When there is fast relative motion between the scene and the video camera, a frame can be distorted because each row was captured under a different 3D-to-2D projection. This kind of distortion is known as rolling shutter effect. Digital video stabilization and rolling shutter rectification seek to remove the unwanted frame-to-frame jitter and rolling shutter effect, in order to generate visually stable and pleasant videos. In general, we need to (1) estimate the camera motion, (2) regenerate camera motion, and (3) synthesize new frames. This dissertation aims at improving the first two steps of video stabilization and rolling shutter rectification. It has been shown that the inertial sensors in handheld devices can provide more accurate and robust motion estimation compared to vision-based methods. This dissertation proposes an online camera-gyroscope calibration method for sensor fusion while a user is capturing video. The proposed method uses an implicit extended Kalman filter and is based on multiple-view geometry in a rolling shutter camera model. It is able to estimate the needed calibration parameters online with all kinds of camera motion. Given the camera motion estimated from inertial sensors after the pro- posed calibration method, this dissertation first proposes an offline motion smoothing algorithm based on a 3D rotational camera motion model. The offline motion smoothing is formulated as a geodesic-convex regression problem on the manifold of rotation matrix sequences. The formulated problem is solved by an efficient two-metric projection algorithm on the manifold. The geodesic-distance-based smoothness metric better exploits the manifold structure of sequences of rotation matrices. Then this dissertation proposes two online motion smoothing algorithms that are also based on a 3D rotational camera motion model. The first algorithm extends IIR filtering from Euclidean space to the nonlinear manifold of 3D rotation matrices. The second algorithm uses unscented Kalman filtering on a constant angular velocity model. Both offline and online motion smoothing algorithms are constrained to guarantee that no black borders intrude into the stabilized frames. / text

Έχει περάσει περισσότερο από ένας αιώνας από τότε, που ο Χαρίλαος Τρικούπης, Πρωθυπουργός της Ελλάδας οραματίστηκε τη κατασκευή μιας γέφυρας, η οποία θα ένωνε την δυτική Πελοπόννησο με την ηπειρωτική Ελλάδα, το Ρίο με το Αντίρριο. Την εποχή εκείνη, τα τρία χιλιόμετρα θάλασσας, που μεσολαβούσαν μεταξύ των δυο πόλεων, φάνταζαν αδύνατο να γεφυρωθούν. Οι δυσκολίες ήταν πάρα πολλές και η τεχνογνωσία δεν είχε ακόμη φτάσει σε τέτοια επίπεδα, έτσι ώστε να δώσει πνοή σ’ αυτό το μεγαλόπνοο όραμα. Τελικά, στα μέσα της δεκαετίας του 90’, μια γαλλοελληνική σύμπραξη, αποτελούμενη από όμιλο εταιριών και από τις δυο χώρες, ανέλαβε τη σχεδίαση και κατασκευή της γέφυρας. Τα έργα ξεκίνησαν τον Ιούλιο του 1998 υπό την επίβλεψη και καθοδήγηση του αρχιτέκτονα Berdj Mikaelian. Η κατασκευή της γέφυρας αναμένετο να ολοκληρωθεί το χρονικό διάστημα μεταξύ Σεπτεμβρίου και Νοεμβρίου του 2004, άλλα οι εργασίες επισπεύτηκαν ένεκα των Ολυμπιακών Αγώνων, που θα λάβαιναν χώρα στην Αθήνα την ίδια χρονιά. Η γέφυρα θεωρήθηκε ως ένα θαύμα της σύγχρονης μηχανικής και όλα τα απαραίτητα μέτρα είχαν ληφθεί για την άρτια λειτουργία της. Παρ’ όλα αυτά στις 27 Ιανουαρίου του 2005, έξι μόλις μήνες μετά τα εγκαίνια, ένας κεραυνός έπληξε ένα από τα καλώδια στήριξης, τα οποία ενώνουν το κατάστρωμα της γέφυρας με τους πυλώνες. Το υψηλής περιεκτικότητας σε πολυαιθυλένιο καλώδιο τυλίχτηκε στις φλόγες με αποτέλεσμα να καταστραφεί ολοσχερώς και να καταρρεύσει στο κατάστρωμα. Όλες οι απαραίτητες ενέργειες έγιναν ταχύτατα, έτσι ώστε να αντικατασταθεί το καλώδιο και να παραδοθεί η γέφυρα και πάλι στη κυκλοφορία το συντομότερο δυνατό. Όμως πλέον ήταν φανερό ότι η αντικεραυνική προστασία της γέφυρας δεν ήταν αρκετή και αποτελεσματική. Στη συνέχεια του κειμένου θα γίνει ανάλυση του παραπάνω συμβάντος, αφού πρώτα γίνει αναφορά σε κάποιες βασικές αρχές γύρω από το φαινόμενο του κεραυνού και των συστημάτων αντικεραυνικής προστασίας. / More than a century has passed, since Charilaos Trikoupis, Prime Minister of Greece had contemplated the construction of a bridge that would connect western Peloponnese with the mainland of Greece, the city of Rion with Antirio. Back then, the three kilometers of sea water which separated the two cities, seemed impossible to be bridged. The difficulties were many and the know how had not reached that level, where it would make a great vision such as this, possible. Eventually, in the mid 90s, a greek-french collaboration, composed by a group of companies from both countries, took over the design and the building of the bridge. Construction works started in July of 1998 under the supervision and guidance of the architect Berdj Mikaelian. The construction of the bridge was expected to be completed during the period of September to November of 2004, but works were accelerated because of the Olympic games that would take place in Athens, that same year. The bridge was considered to be a miracle of modern mechanics and all the necessary measures were taken for its perfect operation. However, on January 27 of 2005, just six months after the opening of the bridge, a lightning stroke cut down one of the longest stay cables that connected the deck of the bridge to the pylons. The high density polyethylene cable was set on fire, and as a result of that the cable was completely destroyed and fell on the deck. All the necessary means were taken, in order to replace the cable and get traffic back on the bridge as soon as possible. It was obvious at that point that the lightning protection of the bridge was neither sufficient or effective enough. In the following chapters there are an analysis on the lightning incident on the bridge, after mentioning the natural phenomenon of lightning and the definition and the mail principals of the lightning protection system of a structure.

In this work are present analysis of traction rolling – stock in Lithuania. There are traversed main criterias of 2M62 series lokomotyve of Radviliškis lokomotyve depot – expenses of maintenance and outlays of fuel, oil, unplanned repairs, running repairs ER-1 and technical handling TP-3.According to complex quality rate is determined critical technical state of traction rolling-stock. There is designed expedient technique of selection of traction rolling-stock and terms of purchase, invoking which we could get maximum economical effect.Inferences are present in the end of this work.

There exists considerable debate in the texture community about whether grain interactions are a necessary factor to explain the development of deformation textures in polycrystalline metals. Computer simulations indicate that grain interactions play a significant role, while experimental evidence shows that the material type and starting orientation are more important in the development of texture and microstructure. A balanced review of the literature on face-centered cubic metals shows that the opposing viewpoints have developed due to the lack of any complete experimental study which considers both the intrinsic (material type and starting orientation) and extrinsic (grain interaction) factors. In this study, a novel method was developed to assemble ideally orientated crystalline aggregates in 99.99\% aluminum (Al) or copper (Cu) to experimentally evaluate the effect of grain interactions on room temperature deformation texture. Ideal orientations relevant to face-centered cubic rolling textures, Cube $\{100\}\left<001\right>$, Goss $\{110\}\left<001\right>$, Brass $\{110\}\left<1\bar{1}2\right>$ and Copper $\{112\}\left<11\bar{1}\right>$ were paired in different combinations and deformed by plane strain compression to moderate strain levels of 1.0 to 1.5. Orientation dependent mechanical behavior was distinguishable from that of the neighbor-influenced behavior. In interacting crystals the constraint on the rolling direction shear strains ($\gamma_{_{XY}}, \gamma_{_{XZ}}$) was found to be most critical to show the effect of interactions via the evolution of local microstructure and microtexture. Interacting crystals with increasing deformations were observed to gradually rotate towards the S-component, $\{123\}\langle\bar{6}\bar{3}4\rangle$. Apart from the average lattice reorientations, the interacting crystals also developed strong long-range orientation gradients inside the bulk of the crystal, which were identified as accumulating misorientations across the deformation boundaries. Based on a statistical procedure using quaternions, the orientation and interaction related heterogeneous deformations were characterized by three principal component vectors and their respective eigenvalues for both the orientation and misorientation distributions. For the case of a medium stacking fault energy metal like Cu, the texture and microstructure development depends wholly on the starting orientations. Microstructural instabilities in Cu are explained through a local slip clustering process, and the possible role of grain interactions on such instabilities is proposed. In contrast, the texture and microstructure development in a high stacking fault energy metal like Al is found to be dependent on the grain interactions. In general, orientation, grain interaction and material type were found to be key factors in the development of rolling textures in face-centered cubic metals and alloys. Moreso, in the texture development not any single parameter can be held responsible, rather, the interdependency of each of the three parameters must be considered. In this frame-work polycrystalline grains can be classified into four types according to their stability and susceptibility during deformation. / Thesis (Ph.D, Mechanical and Materials Engineering) -- Queen's University, 2009-09-25 23:59:11.809

No description available.

Molecular diagnostics is a fast growing field with new technologies being developed constantly. There is a demand for more sophisticated molecular tools able to detect a multitude of molecules on a single molecule level with high specificity, able to distinguish them from other similar molecules. This becomes very important for infectious diagnostics with the increasing antibiotic resistant viruses and bacteria, in gene based diagnostics and for early detection and more targeted treatments of cancer. For increased sensitivity, simplicity, speed and user friendliness, novel readouts are emerging, taking advantage of new technologies being discovered in the field of nanotechnology.  This thesis, based upon four papers, examines two novel electrical readouts for amplified single molecule detection. Target probing is based upon the highly specific amplification technique rolling circle amplification (RCA). RCA enables localized amplification resulting in a long single stranded DNA molecule containing tandem repeats of the probing sequence as product. Paper I demonstrates sensitive detection of bacterial genomic DNA using a magnetic nanoparticles-based substrate-free method where as few as 50 bacteria can be detected. Paper II illustrates a new sensor concept based on the formation of conducting molecular nanowires forming a low resistance circuit. The rolling circle products are stretched to bridge an electrode gap and upon metallization the resistance drops by several orders of magnitude, resulting in an extremely high signal to noise ratio. Paper III explores a novel metallization technique, demonstrating the efficient incorporation of boranephosphonate modified nucleotides during RCA.  In the presence of a silver ion solution, defined metal nanoparticles are formed along the DNA molecule with high spatial specificity. Paper IV demonstrates the ability to manipulate rolling circle products by dielectrophoresis. In the presence of a high AC electric field the rolling circle products stretch to bridge a 10 µm electrode gap.

<p>The main objectives of this research are: (i) to elaborate a unified nonlinear viscoelastic model for rubber-like materials, in finite strain, accounting for material softening under deformation, and for damage induced anisotropy, (ii) to conceive, implement and test, simple, robust and efficient frictional rolling and sliding contact algorithms, in steady-state, as alternatives to existing, general purpose, contact solving strategies, (iii) to develop and verify high fidelity and computationally efficient modeling tools for isotropic and anisotropic viscoelastic objects in steady-state motion, (iv) to investigate, numerically and through experimentation, the influence of various material parameters, including material nonlinearities such at the Payne effect and the Mullins effect, as well as geometric parameters and contact surface conditions, on viscoelastic rolling resistance, and (iv) to explore, analytically and through experimentation, the conditions under which favorable mechanical synergies occur between material components and develop novel composites with improved structural performances.</p><p>A new constitutive model that unifies the behavioral characterizations of rubber-like materials in a broad range of loading regimes is proposed. The model reflects two fundamental aspects of rubber behavior in finite strain: (i) the Mullins effect, and (ii) hyper-viscoelasticity with multiple time scales, including at high strain rates. Suitable means of identifying the system's parameters from simple uniaxial extension tests are explored. A directional approach extending the model to handle softening induced anisotropy is also discussed.</p><p>Novel, simple, and yet robust and efficient algorithms for solving steady-state, frictional, rolling/sliding contact problems, in two and three dimensions are presented. These are alternatives to powerful, well established, but in particular instances, possibly `cumbersome' general-purpose numerical techniques, such as finite-element approaches based on constrained optimization. The proposed algorithms are applied to the rolling resistance of cylinders and spheres.</p><p>Two and three-dimensional boundary element formulations of isotropic, transversely isotropic, and fully orthotropic, compressible and incompressible, viscoelastic layers of finite thickness are presented, in a moving frame of reference. The proposed formulations are based on two-dimensional Fourier series expansions of relevant mechanical fields in the continuum of the layers and support any linear viscoelastic material model characterized by general frequency-domain master-curves. These modeling techniques result in a compliance matrix for the upper boundary of the layers, including the effects of steady-state motion. Such characterizations may be used as components in various problem settings to generate sequences of high fidelity solutions for varying parameters. These are applied, in combination with appropriate contact solvers, to the rolling resistance of rigid cylinders and spheres.</p><p>The problem of a viscoelastic sphere moving across a rigid surface is significantly more complicated than that of a rigid indenter on a viscoelastic plane. The additional difficulties raised by the former may explain why previous work on this topic is so sparse. A new boundary element formulation for the multi-layered viscoelastic coating of a rigid sphere is developed. The model relies on the assumption of a relatively small contact surface in order to decouple equilibrium equations in the frequency domain. It is applied in combination with an adapted rolling contact solving strategy to the rolling resistance of a coated sphere.</p><p>New modeling approaches yielding rolling resistance estimates for rigid spheres (and cylinders) on viscoelastic layers of finite thicknesses are also introduced, as lower-cost alternatives to more comprehensive solution-finding strategies, including those proposed in this work. Application examples illustrate the capabilities of the different approaches over their respective ranges of validity.</p><p>The computational tools proposed in this dissertation are verified by comparison to dynamic finite element simulations and to existing solutions in limiting cases. The dependencies of rolling resistance on problem parameters are explored. It is for instance shown that, on orthotropic layers, the dissipated power varies with the direction of motion, which suggests new ways of optimizing the level of damping in various engineering applications of very high impact. Interesting lateral viscoelastic effects resulting from material asymmetry are unveiled. These phenomena could be harnessed to achieve smooth and `invisible' guides across three-dimensional viscoelastic surfaces, and hence suggest new ways of controlling trajectories, with a broad range of potential applications.</p><p>A new experimental apparatus is designed and assembled to measure viscoelastic rolling resistance. Experiments are conducted by rolling steel balls between sheets of rubber. Principal sources of measurement error, specific to the device, are discussed. Rolling resistance predictions are obtained using the computational tools presented in this dissertation, and compared to the measurements. Interesting conclusions are drawn regarding the fundamental influence of the Payne effect on viscoelastic rolling friction.</p><p>The work presented in this dissertations finally touches on the mechanical behavior of casing-infill composite tubes, as potential new lightweight structural elements. The axial behavior of composite circular tubes is addressed analytically. The influence of material parameters and geometry on structural performances are revealed and presented in original graphical forms. It is for instance shown that significantly improved overall stiffness and capacity at yield can be obtained using a moderately soft and highly auxetic infill, which further highlights the need to develop new lightweight auxetic materials, without compromising their stiffness. It is furthermore concluded that limited mechanical synergies can be expected in metal-polymer composite tubes, within the linear range of the materials involved. This prediction is confirmed by a bending experiment conducted on an Aluminum-Urethane composite tube. The experiment however reveals unexpected and quite promising mechanical synergies under large deformations. This novel composite has a potential influence on the design and performance of lightweight protecting structures against shocks and accelerations due to impacts, which justifies that it be characterized further.</p> / Dissertation

It is well known that cells in tissues display a large heterogeneity in gene expression due to differences in cell lineage origin and variation in the local environment at different sites in the tissue, a heterogeneity that is difficult to study by analyzing bulk RNA extracts from tissue. Recently, genome-wide transcriptome analysis technologies have enabled the analysis of this variation with single-cell resolution. In order to link the heterogeneity observed at molecular level with the morphological context of tissues, new methods are needed which achieve an additional level of information, such as spatial resolution. In this thesis I describe the development and application of padlock probes and rolling circle amplification (RCA) as molecular tools for spatially-resolved transcriptome analysis. Padlock probes allow in situ detection of individual mRNA molecules with single nucleotide resolution, visualizing the molecular information directly in the cell and tissue context. Detection of clinically relevant point mutations in tumor samples is achieved by using padlock probes in situ, allowing visualization of intra-tumor heterogeneity. To resolve more complex gene expression patterns, we developed in situ sequencing of RCA products combining padlock probes and next-generation sequencing methods. We demonstrated the use of this new method by, for the first time, sequencing short stretches of transcript molecules directly in cells and tissue. By using in situ sequencing as read-out for multiplexed padlock probe assays, we measured the expression of tens of genes in hundreds of thousands of cells, including point mutations, fusions transcripts and gene expression level. These molecular tools can complement genome-wide transcriptome analyses adding spatial resolution to the molecular information. This level of resolution is important for the understanding of many biological processes and potentially relevant for the clinical management of cancer patients. / <p>At the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 4: Manuscript.</p>