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Production and Purification of Synthetic Minor Ampullate Silk ProteinsGaztambide, Danielle A. 01 December 2018 (has links)
Spider silks are incredible natural materials that have a wide variety of properties that can rival or outperform even common synthetic materials like Nylon and Kevlar. As nature’s architects, orb-weaving spiders spin seven different silks that are used for very specific roles throughout the spider’s lifecycle. These silks are comprised of proteins called spidroins. Each of these spidroins has evolved to have properties such as strength and/or stretch that make these silks successful and highly adapted in their designated roles in web construction, prey capture and reproduction.
This study involves the production of minor ampullate silk by genetically modifying the bacteria Escherichia coli. Minor ampullate is a lesser studied silk that is used for the first spiral of the orb web. This spiral is a template that the spider uses to finish the web and provides stability during the web construction. Minor ampullate silk is strong, however it does not stretch so it may be well-suited for certain applications such as ballistic materials.
By producing and purifying different arrangements of minor ampullate silk protein, it is possible to learn how this protein can be expressed without using the spider itself. This investigation sheds light on how deviations in the protein sequence and motif arrangement can produce different properties, which can potentially be used to make new materials.
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Cloning and expression of a cunner-fish trypsin in bacteria and yeastMacouzet-García, Martin January 2004 (has links)
No description available.
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Development of Methylobacterium extorquens as a recombinant protein production system and the expression of the heterologous cry1Aa gene from Bacillus thuringiensisBélanger, Louise January 2003 (has links)
No description available.
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Drought Tolerance Compared Between Two Eutrema salsugineum Ecotypes and Their Recombinant Inbred LinesJennifer Tropiano January 2021 (has links)
Despite drought accounting for over 80% of agricultural losses, little progress has
been made towards improving drought tolerance in crops. My approach to identifying
traits underlying drought tolerance involved a comparison between two accessions of
the crucifer, Eutrema salsugineum, that display differential tolerance to water deficits.
The accessions, originating from the semi-arid Yukon, Canada, and a monsoonal region
of Shandong, China, were subjected to a two-step, water deficit and recovery
protocol to identify physiological characteristics that discern their drought-responsive
behaviour. Traits that discriminate between the ecotypes were used to screen recombinant
inbred lines (RILs) that were generated by crossing Yukon and Shandong
parent plants. Selected physiological measurements were: anthocyanin accumulation,
cut rosette water loss (CRWL), solute potential, relative water content (RWC), static
leaf water content (SLWC), specific leaf area (SLA), and OJIP fluorescence emission.
Of the measurements taken, CRWL measurements and anthocyanin content
distinguished the Yukon ecotype from the Shandong ecotype during the first drought
exposure whereas SLA and fluorescence responses differentiated these accessions better
after plants that experienced the first drought were rewatered and recovering or
undergoing a second drought treatment. Sixty-eight RILs were screened using SLA
and OJIP fluorescence emission. SLA and OJIP measurements varied among the
recombinant inbred lines (RILs) with many lines showing responses to water deficit
intermediate to those of the parental lines. Evidence of heritability in SLA and/or
OJIP responses to water deficits would make them useful phenotypic markers for
identifying quantitative trait loci (QTLs) associated with drought tolerance in future
work. / Thesis / Master of Science (MSc)
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Expression and characterization of recombinant nerve growth factorLuo, Yuling January 1992 (has links)
No description available.
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The Effects of Recombinant Osteoactivin on Murine OsteoclastogenesisKhol, Matthew Philip January 2014 (has links)
No description available.
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The role of innate immunity in protection against respiratory syncytial virus (RSV)Vaghefi, Negin Gitiban 22 February 2006 (has links)
No description available.
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Geração e análise da imunogenicidade de proteínas recombinantes baseadas nas diferentes formas do antígeno circumsporozoíta de Plasmodium vivax visando o desenvolvimento de uma vacina universal contra malária. / Generation and analysis of the immunogenicity of recombinant proteins based on different forms of the circumsporozoite antigen of Plasmodium vivax for the development of a universal vaccine against malaria.Teixeira, Lais Helena 26 March 2014 (has links)
O P. vivax é a segunda espécie mais prevalente causadora de malária no mundo. Medidas de controle ineficientes exigem o desenvolvimento de novas estratégias de prevenção, como vacinas, novas drogas e novos inseticidas. O objetivo geral do trabalho foi gerar uma formulação vacinal universal com proteínas e adenovírus recombinantes capazes de induzir anticorpos contra as diferentes formas alélicas da proteína circumsporozoíta (CSP) do P. vivax. As proteínas foram produzidas em E. coli e purificadas por cromatografia de afinidade e troca iônica. A obtenção destas proteínas nos permitiu testar qual seria a melhor formulação vacinal para a indução de anticorpos contra as três formas alélicas da proteína CSP de P. vivax (PvCSP). Anticorpos específicos reconheceram esporozoítas do P. vivax por imunofluorescência. Por fim testamos o uso de dois adenovírus recombinantes, um símio e um humano, deficientes em replicação, expressando as três regiões imunodominantes da proteína PvCSP em fusão. Estes foram capazes de induzir resposta imune específica contra as proteínas PvCSP sendo testados em esquema de prime-boost heterólogo, onde camundongos foram primados com os adenovírus e nas doses-reforço receberam a mistura com as três proteínas recombinantes. / The Plasmodium vivax is the second most prevalent species of malaria in the world. Inefficient measures of control used today demand the development of new strategies for prevention, as vaccines, new drugs and new insecticides. The central objective of this thesis was to generate a universal vaccine formulation with proteins and recombinant adenoviral vectors representing the different allelic forms of the circumsporozoite protein (CSP) of the P. vivax. The recombinant proteins were expressed in E. coli and purified. These proteins allowed us to test which would be the best vaccine formulation for the induction of antibodies against the three allelic forms of CSP. The specific antibodies also recognized P. vivax sporozoites by immunofluorescence. Finally we test the use of two recombinant adenoviral vectors, a simian and a human, both replication deficient, expressing a protein containing the repeat regions of the CSP in fusion. These adenoviral vectors induced specific immune response against CSP and were successfully used in an immunization regimen of heterologous prime and boost where in the first dose the mice received recombinant adenoviral vector and in the subsequent doses, the mixture with three recombinant proteins.
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Validación y caracterización de un método inmuno-electroforético para la detección de eritropoyetina recombinante y análogosBelalcazar Guerrero, Viviana 14 December 2007 (has links)
El objetivo principal de esta tesis fue el de realizar una validación y caracterización de las posibles variables que pueden afectar la metodología empleada para identificar las isoformas presentes en algunas de las especies de EPO (uEPO, rEPO alfa, rEPO beta y el NESP) tras su identificación mediante la técnica de IEF e inmunodetección
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Genetic engineering of non-beta-cells for regulated insulin secretionTang, Shiue-Cheng 01 December 2003 (has links)
No description available.
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