The membrane bioreactor (MBR) is becoming increasingly popular for wastewater treatment, mainly due to its capability of producing high quality effluent with a relatively small footprint. However, high plant maintenance and operating costs due to membrane fouling limit the wide spread application of MBRs. Membrane fouling generally depends on the interactions between the membrane and, the activated sludge mixed liquor, which in turn, are affected by the chosen operating conditions. The present research study aimed to explore the process performance and membrane fouling in the membrane enhanced biological phosphorus removal (MEBPR) process under different operating conditions by, (1) comparing two MEBPRs operated in parallel, one with constant inflow and another with a variable inflow, and by, (2) operating the MEBPRs with different solids retention times (SRT). On-line filtration experiments were conducted simultaneously in both MEBPR systems by using test membrane modules. From the transmembrane pressure (TMP) data of the test membrane modules, it was revealed that fouling propensities of the MEBPR mixed liquors were similar in both parallel reactors under the operating conditions applied, although the fouling propensity of the aerobic mixed liquors of both reactors increased when the SRT of the reactors was reduced. Routinely monitored reactor performance data suggest that an MEBPR process with a varying inflow (dynamic operating condition) performs similarly to an MEBPR process with steady operating conditions at SRTs of 10 days and 20 days. Mixed liquor characterization tests were conducted, including critical flux, capillary suction time (CST), time to filter (TTF) and, bound and soluble extracellular polymeric substances (EPS) were quantified, to evaluate their role on membrane fouling. The tests results suggest that the inflow variation in an MEBPR process did not make a significant difference in any of the measured parameters. With decreased SRT, an increase in the concentrations of EPS was observed, especially the bound protein, and the bound and soluble humic-like substances. This suggests that these components of activated sludge mixed liquors may be related to membrane fouling. No clear relationship was observed between membrane fouling and other measured parameters, including critical flux, normalized CST and normalized TTF. / Applied Science, Faculty of / Civil Engineering, Department of / Graduate

The waste water from the industrial production of the herbicide monosodium methyl arsenate was treated using coagulation. The coagulation process as developed in this research proved to be suitable for arsenic removal in aqueous media using chromium (III), calcium (II), and combination of calcium (II) and chromium (III), and magnesium (II). The results obtained suggest that the coagulation process can be used for the treatment of the waste water from the monosodium methyl arsenate production. Response surface methodology was used to study the effects of the various parameters, namely pH, mole ratios (Cr:As, Ca:As, and Mg:As), concentration of flocculent and initial arsenic concentration. To optimize the process conditions for the maximum removal of arsenic. Central composite and factorial designs were used to study the effects of these variables and to predict the effect of each. ANOVA was used to identify those factors which had significant effects on model quality and performance. The initial arsenic concentration appeared to be the only significant factor. These models were statistically tested and verified by confirmation experiments.

This master thesis project was carried out as a part of the chlorate research conducted at the Process RD&I department for bleaching chemicals at AkzoNobel Pulp and Performance Chemicals AB in Bohus. During the project already implemented filter cloths as well as new types of filters were studied and compared by experimental trials. The results were then examined in an attempt to evaluate existing filtration systems as well as investigate if there are other, better alternatives. The impurities found in a chlorate plant account for an efficiency loss in the process and a reduction of impurities would hence result in an energy reduction and a cleaner product. The trials were conducted at one of AkzoNobel’s chlorate plants. Six filters were studied and evaluated by measuring turbidity of the electrolyte and pressure over the filter during the experiments. Samples of the electrolyte were analyzed to obtain the metal content, and thereby the impurity content, of the electrolyte. The structures of the filters were studied by optical microscopy. The results from the trials show that all filter types except one, a needle felt filter, seem to be suitable for chlorate electrolyte filtration (including the filter types already used in the plants). The other filters all reach turbidity values below 0.1 FNU immediately or within 90 minutes of filtration, which is considered good enough. The results from the metal content analysis show a similar trend where the metal concentrations decrease to levels below the detection limits immediately or within 90 minutes of filtration. Apart from the lab trials performed some measurements were made on the existing filtration equipment in the chlorate plant. The measurements show varying results, partly similar to those achieved during the lab filter trials but also results showing a higher turbidity value and metal content, indicating that full scale operation are more complicated than lab scale operation. The lab trial results obtained with the filter types already used in the plants show that lower impurity content is possible to achieve. However, this would require closer monitoring of the filtration systems in the plants. Apart from the filtration trials, an attempt to determine the sizes of the particles present in the electrolyte using laser diffraction was performed. However, too little was known of the chlorate electrolyte’s optical data for the measurements to be reliable. Further work is needed before a method for size determination of the particles in a chlorate electrolyte can be achieved. Also, an Optical Filtration Test was tried on the electrolyte but was not sensitive enough for utilization on electrolyte with low (below 1 FNU) turbidity values.  The project concluded that a switch to another filtration system is unmotivated, unless a change in the product requirements would occur. Since the impurities have proven to affect the efficiency of the process, it is recommended to make an effort into utilizing the filtration system to its full extent.

Biological nutrient removal (BNR) from wastewater, and specifically nitrogen removal, is a growing concern to wastewater dischargers such as municipalities. Excess nutrients in effluent can create problems such as eutrophication, toxicity to aquatic life, and dissolved oxygen depletion in receiving waters. BNR systems have been installed in many locations with success, but their operation presents operational and financial demands greater than conventional biological treatment. Nitrogen removal is typically performed in sequential autotrophic nitrification and denitrification, which increases needed energy input, operational complexity, and therefore cost. Simultaneous nitrification-denitrification (SNdN) achieved in a single system has also been successfully implemented, however operational parameters that compromise between ideals for aerobic nitrification and anoxic denitrification result in decreased reaction rates and removal efficiencies. The application of a product that could potentially enhance SNdN reaction rates and removal efficiencies through bioaugmentation could help ease operational and financial strains. In contrast to common sequential processes, some heterotrophic Bacillus bacteria have demonstrated SNdN (Kim et al., 2005), (Zhang et al., 2011). However, their application outside of laboratory setting has yet to be established. Aqua™ is a proprietary bioaugmentation product composed of specific Bacillus strains developed by BiOWiSH® Technologies with the intent of improving aerobic, heterotrophic SNdN rates and removal efficiencies. Screening and bench-scale experiments were performed in flasks at 35° C on orbital shakers operated at a range of speeds. Primary wastewater and minimal media were used for the experiment, and inoculation was performed with both specific Bacillus strains and Aqua™. Rapid total ammonia nitrogen (TAN) removal was observed in initial screening experiments with Aqua™ in sterile wastewater. Bacillus pumilus was identified as the fastest growing organism of the Aqua™ assemblage with the greatest TAN removal 1st order rate constant (0.32/ hr.), decreasing TAN 96% within 10 hours from an initial 48.5 ppm. The orbital shaker speed that maximized TAN removal was 100 rpm, with reduction 47% and 88% more effective than both the upper (150 rpm) and lower (50 rpm) bound tested speeds, respectively. Visible floc growth centered in flasks, along with optical density data indicated cell growth and the possibility the system could support SNdN. Carbon amendments to minimal media were then evaluated, and sodium succinate improved TAN reduction by 53% compared to dextrose amended systems. This was likely because dextrose metabolism requires glycolysis to produce pyruvate for utilization in the TCA cycle for energy production; while succinate avoids glycolysis and thus is more easily utilized. In another experiment, flasks with supplemental trace minerals had a 59% higher TAN removal than the controls. Additions of supplemental vitamin solution or yeast extract improved TAN removal by 18% and 38%, respectively. Two 10-day experiments assessed Aqua™ performance in municipal primary clarifier effluent. Nitrogen balance and optical density data showed that Aqua™ dosing at 10 ppm had no effect on nitrogen removal. The second 10-day experiment increased Aqua™ dosing to 50 ppm and evaluated product activation through incubation in growth media prior to inoculation. Nitrogen balance analysis showed no effect from Aqua™ on nitrogen removal during the second 10-day experiment as well. Systems amended with dextrose saw an initial rapid TAN first order removal rate (0.25/ hr.). However, difference between control and inoculated flasks was negligible showing no effect from Aqua™. A lack of total nitrogen losses and a lack of nitrate presence during initial rapid TAN losses confirmed these losses were by assimilation into organic nitrogen. The above experiments suggest that initial success in TAN removal during screening experiments resulted from lack of competition with other microorganisms, the high 1500 ppm dose of Aqua™, and amended dextrose.

Discharging cheese production wastewater with high concentrations of organics and nutrients is detrimental to receiving aquatic systems, as the release of these deleterious substances cause oxygen depletion, and eutrophication respectively. On-site treatment of cheese production wastewater requires the removal of high concentrations of organics and nutrients with a small land footprint to meet regulations. There is therefore a critical need for compact, high-rate, cost-effective wastewater technologies such a as the moving bed biofilm reactor (MBBR). Although MBBR systems have been well established for carbon and nitrogen removal, to date only a limited number of studies have achieved enhanced biological phosphorous removal in sequencing batch moving bed biofilm reactor (SB-MBBR) systems, and only for municipal-strength wastewater. Operating SB-MBBR systems under sequencing batch mode enables the reactor operation to be well synced to the fluctuating influent concentrations and flow characteristics of cheese production wastewaters. Furthermore, cycling between anaerobic and aerobic conditions can be achieved in a single sequencing batch reactor, which can promote the proliferation of poly-phosphate accumulating organisms. The SB-MBBR is studied in this research for the removal of carbon, nitrogen, and phosphorous from cheese production wastewaters. Specifically, the effects of anaerobic staging time, aeration rate, enhanced aerobic operation, and adding a second reactor in series was studied by analyzing the kinetics, biofilm characteristics, and microbiome. Extending the anaerobic staging time was shown to achieve aerobic soluble chemical oxygen demand removal rates of 92.5±2.8 g·m⁻²d⁻¹, by selecting for a thinner biofilm with, with a lower biofilm dry-density and a more rough biofilm surface, and therefore likely a biofilm with an enhanced mass transport. A significant shift in the microbiome was observed with longer anaerobic staging times and lower aeration, whereby possible putative poly-phosphate accumulating organisms including Brachymonas, and Dechloromonas were selected for in greater relative abundances compared to anaerobic bacteria. The total phosphorous removal in the possibly resulted from enhanced biological phosphorous removal, supported by the high abundance of putative poly-phosphate accumulating organisms (43.1±8.4%), which dominated the biofilms in the SB-MBBRs with 120 and 168 minute anaerobic staging times. Finally, total ammonia nitrogen oxidation was achieved through partial nitritation with a two reactor in series configuration with a removal rate of 1.07±0.05 g-N·m⁻²d⁻¹. Two SB-MBBRs operated in series was shown to be the superior strategy for achieving TAN compared to a single SB-MBBR with extended aerobic operation. By operating two SB-MBBRs in series, competition between autotrophic nitrifiers and heterotrophs is averted, and AOB proliferate in the biofilm, achieving TAN oxidation. Since TAN oxidation is likely achieved through partial nitrification, the SB-MBBR technology may be incorporated in a deammonification treatment train. The overall study presents novel information for the SB-MBBR design and operation, along with biofilm and microbiome fundamental findings that will guide future pilot- and full-scale applications of the SB-MBBR to treat cheese production wastewater.

Currently, the Low Earth Orbit (LEO) space environment contains a growing number of orbital debris objects. This growing orbital debris population increases collision probabilities between both orbital debris and functioning satellites. A phenomenon known as Kessler Syndrome can be induced if these collisions occur. Kessler Syndrome states that these collisions can lead to an exponential increase in the orbital debris population, which could dangerously impede future space missions. Current literature outlines the necessity of stabilizing the near-Earth environment debris population and introduces the concept of active debris removal (ADR). The use of ADR on five orbital debris objects per year was found to be a requirement to achieve stability within the orbital debris population. A viable mission architecture is henceforth explored to utilize ADR for near-future execution to further develop research for orbital debris mitigation missions. The larger orbital debris objects are found in many different orbital regimes and are primarily composed of spent rocket bodies and retired satellites. Different orbital debris ranking schemes have been developed based on the population density in these different regimes, which are linked to higher collision probabilities. Using these ranking schemes, a set of target objects are selected to be investigated for this mission design that was composed of target objects with similar orbital characteristics that were not launched by the Commonwealth of Independent States (CIS) to minimize legal barriers. Different ADR capture and removal methods are inspected to find the optimal methods for this mission. An Analytical Hierarchy Process (AHP) has been used to assess these different methods, which utilizes comparisons of the different methods among a set of weighted criteria. A net capture method with a low thrust chemical engine for removal is identified as the optimal ADR method. The use of a laser detumbling system is also selected to stabilize target objects with a high rotation rate. A rendezvous and deorbit orbital analysis are conducted using both a low fidelity tool (for preliminary results) and a high fidelity tool (for more precise results). The rendezvous analysis is used to select a mission architecture that was composed of two different chaser satellites which rendezvous with the five different target objects by taking advantage of nodal precession. The deorbit analysis investigates different decay timelines and found the delta-v estimates that would be required to deorbit the target objects within the same year that they were captured in. These two orbital analyses provide valuable insight to the mission timeline, delta-v estimates, and approximate mass requirement for the chaser satellite and deorbit kits. The results of the target selection process, ADR selection process, and the rendezvous and deorbit analyses are meant to provide an initial concept and analysis for a near-future ADR mission. These approximate results provide insight and information to further develop orbital debris mitigation research to help solve the orbital debris population growth challenge for future space missions. / Master of Science / Currently, the near Earth space environment contains a growing number of space debris. This growth in the orbital debris population increases the likelihood of collisions with orbital debris, functioning satellites, and launch vehicles. These collisions can generate a chain of events that could exponentially increase the population of orbital debris, which at some scale could become a major obstacle for future space missions. Researchers have introduced the concept of active debris removal (ADR), which in simulations has been shown to help stabilize the growth of orbital debris. The use of ADR to remove as low as five orbital debris objects per year has been found to be sufficient to stabilize debris growth. A viable mission architecture using ADR technologies that can be implemented in the near future is henceforth explored to further develop research for orbital debris missions. The larger orbital debris objects are found in many different areas in space and are primarily made up of used rocket bodies and retired satellites. Different ranking schemes have been developed by researchers for these larger orbital debris objects based on the population density within these areas in space, which are linked to the chance of a collision. Using these ranking schemes, a set of orbital debris objects are selected to be targeted for this mission design. This set of selected target objects have similar orbital characteristics and the political/legal barriers that could be present during removal are minimal. An ADR mission is composed of two primary components, a capture method and a removal method, which are inspected to find the optimal methods for this mission. A decision-making technique, called an Analytical Hierarchy Process (AHP), has been used to assess these different methods. The AHP compares different capture and removal methods using a set of weighted criteria. A net capture method with small thrusters for removal is identified as the optimal ADR method. Additionally, the use of a laser system is selected to stabilize target objects that may be rotating too quickly for capture. An analysis on different mission architectures is conducted using both a low fidelity tool (for preliminary results) and a high fidelity tool (for more precise results). A mission architecture composed of two different "chaser" satellites which rendezvous with and deorbit the five different target objects is selected. The analysis used on the selected mission architecture provides valuable insight to the mission timeline, fuel estimates, and approximate mass requirements. The results of the target selection process, ADR selection process, and the mission architecture analysis are meant to provide an initial concept and introduce possible requirements for a nearfuture ADR mission. These approximate results provide information to further develop research that can help us solve the orbital debris population growth challenge for future space missions.

Tars have been identified as one of the major impurities associated with the utilization of biomass gasification fuel gas. Tars may result in blockages, plugging, corrosion and catalyst deactivation, leading to serious operational and maintenance problems during biomass gasification. Therefore, tar removal is essential to insure economic and effective fuel gas utilization. This study investigates the catalytic activity of zeolites, and nickel-supported zeolites for tar removal. Tests were conducted using a bench scale reactor and naphthalene as a model tar compound. Zeolites with varying pore sizes and acidity were tested to evaluate the effect of pore size and acidity on tar removal. Test results suggested that the catalytic activity increased with an increase in pore size and the number of acidic sites on the zeolite. The steam reforming ability of nickel towards naphthalene removal was evaluated by impregnating nickel on zeolites; this impregnation improved the activity of the catalysts significantly. Long term catalytic activity tests were performed, which showed that nickel supported ZY-30 and ZY-80 had the best naphthalene conversion, with naphthalene conversions of greater than 99%, followed by nickel- supported ZY-5.2, SiO2/Al2O3, and chabazite, respectively. This study also evaluated the catalytic activity of montmorillonite and nickel- supported montmorillonite as tar removal catalysts. Montmorillonite, and Ni-montmorillonite were tested for their efficiency in reforming tars. Also, the efficacy of nickel-supported montmorillonite catalyst was tested as a function of nickel content, reaction temperature, naphthalene loading, and the steam to carbon ratio. The results demonstrated that montmorillonite was catalytically active in removing naphthalene. In addition, Ni-montmorillonite had very high activity towards naphthalene removal via steam reforming, with removal efficiencies of greater than 99%. The activation energy was calculated for Ni-montmorillonite assuming first order kinetics and was found to be 84.5 kJ/mole. Long-term activity tests showed that the catalyst was active with naphthalene removal efficiencies of greater than 95%, which were maintained over a 97-hour test period. Very little loss of activity was observed with a drop in removal from 97 to 95%. The drop in catalytic activity was attributed primarily to a decrease in catalyst surface area, nickel sintering, and coke formation.

With Athletes and individuals developing osteoarthritis and chondral defects at younger ages, long term treatments are in high demand. Total knee replacements only last for 10-15 years, so younger individuals would need to have multiple knee replacements within their lifetime. Allograft transplantation has shown to last long term and have high success rates, but the lack of donors and the possibility of damaging other areas of the knee to obtain tissue grafts has become a large concern. Xenografts derived from porcine cartilage is cost effective and the supply is abundant. Two antigen removal processes were examined: a short term antigen removal process to maintain the mechanical stability of the tissue, and a long antigen removal process to minimize the risk of triggering an immune response. The antigen removal processes were compared, and the future precautions were determined to enhance the probability of creating a viable osteochondral xenograft preparation technique.

This project utilizes hydrocyclones in wastewater treatment to select for heavier solids, and has been used before in multiple small-scale systems. This is the first implementation of hydrocyclones in a full-scale plant for the purpose of increased settleability, while also achieving enhanced biological phosphorus removal without the use of an anaerobic selector. Hydrocyclones receive mixed liquor tangentially and separate light solids from more dense solids through their tapered shape, increasing the velocity of liquid as it moves downward and allowing for selection of a certain solids fraction. The hydrocyclones receive flow from the waste stream, selecting for dense solids to recycle through the process while light solids are wasted, creating a balance of granules and flocs with superior settling characteristics in which phosphorus is removed through phosphorus accumulating organisms (PAO). This project was implemented at a wastewater treatment plant rated at 20 MGD utilizing a 4-stage Bardenpho configuration with an IFAS system. This plant routinely experienced moderate settleability issues with an average SVI of 141 and a 90th percentile SVI of 179. Over time data was collected to characterize settleability and activity of PAO, GAO, and filaments. Using an external selector to achieve biological phosphorus is significant in that most wastewater treatment plants cannot do this without the use of an anaerobic selector. This has the potential to apply external selectors to existing infrastructure throughout plants worldwide to achieve not only biological phosphorus removal, but also improved settleability with a very minor capital investment. / Master of Science

The global spread of COIVD-19, as well as the worsening air pollution throughout the world have brought tremendous attention into the development of materials that can efficiently capture particulate matter (PM). Traditional filters made from fabric, glass fibers, or melt blown fibers exhibit a low efficiency at removing sub-micrometer and nanoscale particles. Additionally, they exhibit limited performance in high humidity, high temperature environments. We suggest that the high porosity of filters composed of nanofibers could provide minimal obstruction to air flow, while their high tortuosity and surface area-to-volume ratio presents an excellent platform for particle capture. Electrospinning is a simple and well-studied process to produce randomly accumulated nano- and micro-scale diameter fibers. The main advantages of electrospun nanofibers include their tunable fiber morphology and diameter under specific electrospinning parameters, as well as the ease of post-process modification. Studies have demonstrated its promising applications ranging from tissue engineering, water purification to wearable electronics. Giving the tunable aspect of the process, various polymers were electrospun with different morphology and fiber diameter which all demonstrated high particle removal efficiency. Cellulose was chosen as the base material for our study since it is the most abundant biopolymer and its affinity for further chemical modification. In this study, the removal of nanoscale particles via in-house fabricated cellulose nanofilters is significantly enhanced by chemically functionalizing the fibers’ surface via the deposition of the bio-inspired glue polydopamine (PDA) and the polycation poly(diallyldimethylammonium chloride) (PDADMAC). Nanofilters were electrospun from cellulose acetate solutions before being regenerated to cellulose via an alkaline treatment. Cellulose nanofilters were then functionalized using only PDA or the codeposition of PDA with PDADMAC. Scanning electron microscope (SEM), Fourier transform infrared spectrometer (FTIR), and high-resolution X-ray photoelectron spectroscopy (XPS) were used to characterize the nanofilters. The effects of filter packing density, filter layering, and surface functionalization on their performance, i.e., their filtration efficiency, most penetrating particle size (MPPS), performance in a high humidity environment, and filter pressure drop were investigated. Furthermore, by introducing hydrophilic and hydrophobic nanofibers within a composite filter structure, the performance of the composite filter remained unchanged even in high humidity.