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Work and absence from workNorth, Fiona Mary January 1990 (has links)
This study assesses risk factors for sickness absence in the British civil service. As one component of a large study of psychosocial factors and health, 10,314 civil servants between the age of 35 and 55 completed questionnaires about their work environment, social circumstances outside work, health and health-related behaviours. To obtain a more objective measure of the work environment, personnel managers provided external assessments of participants' jobs. The baseline variables were related to rates of short spells (7 days or less) and long spells (more than 7 days) of sickness absence for 85% of participants, over a mean period of 20 months (6-26 months). There were striking grade differences in sickness absence, with a stepwise increase in rates of both short and long spells from top administrators to clerical and office support staff. Other identified risk factors explained only a third of these grade differences in sickness absence. Further analyses were adjusted for age and grade. Self-reported health was strongly related to rates of long spells and, to a lesser extent, short spells. Adequacy of support and difficulty paying bills were the two factors outside work which related to rates of both short and long spells. Job satisfaction was the only measure of the work environment which related to rates of both short and long spells. Other aspects of the work environment which were associated with increased rates of short spells were low variety and skill use and low support at work for both sexes, and low control, slow work pace and few conflicting demands for men. Self-reports and external assessments of the work environment related to sickness absence in a similar way, suggesting that the work environment itself was important. Factors which did not relate to either short or long spells of sickness absence were marital status, dependent children, the frequency of social contacts and physical activity. Women had higher rates of sickness absence than men and Asians had higher rates than Caucasians. This study identified a number of risk factors for sickness absence and differences in these risk factors for short and long spells of sickness absence. The grade, sex and ethnic differences in sickness absence remained largely unexplained. Group attitudes towards sickness absence may be important. Methodological issues related to the assessment of psychosocial factors are discussed.
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Digital diffusion analogue /Svilans, Mikelis Nils. January 1974 (has links) (PDF)
Thesis (M.E. 1975) from the Department of Electrical Engineering, University of Adelaide.
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Silencing African horsesickness virus VP7 protein expression in vitro by RNA interferenceBurger, Liesel January 2006 (has links)
Thesis (M.Sc.(Microbiology)--University of Pretoria, 2006. / Summary in English. Includes bibliographical references.
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Mutational analysis of a gene required for flagellar motility in the African sleeping sickness parasite /Dantas, Sonia N. January 2008 (has links) (PDF)
Undergraduate honors paper--Mount Holyoke College, 2008. Dept of Biological Sciences. / Includes bibliographical references (leaves 64-69).
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The characterization of inner core protein VP6 of African horsesickness virusDe Waal Pamela Jean. January 2005 (has links)
Thesis (Ph. D.)(Genetics)--University of Pretoria, 2005. / Includes summary. Includes bibliographical references. Available on the Internet via the World Wide Web.
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Mutational analysis of T. brucei components of motile flagella (TbCMF) genes in the African trypanosome /Hare, Julie D. January 2007 (has links) (PDF)
Undergraduate honors paper--Mount Holyoke College, 2007. Program in Biochemistry. / Includes bibliographical references (leaves 54-58).
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Temperature and the transmission of arboviruses by Culicoides biting midgesWittmann, Emma Jane January 2000 (has links)
No description available.
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Silencing of African horse sickness virus NS2 gene expression using vector-derived short hairpin RNAsNieuwoudt, Marthi Andrea 18 November 2008 (has links)
African horse sickness virus (AHSV), a member of the Orbivirus genus within the Reoviridae family, has a 10-segment double-stranded (ds)RNA genome enclosed within a double capsid. In addition to seven structural proteins (VP1-VP7), four non-structural proteins (NS1, NS2 and NS3/NS3A) are synthesized in infected cells and are involved in virus morphogenesis. Due to the lack of a reverse genetic system for orbiviruses, analyses regarding AHSV gene function have been limited to the characterization of individual virus proteins following their expression in heterologous expression systems. The phenomenon of RNA interference (RNAi), has, however, revolutionized approaches to study the function of individual genes. RNAi is an evolutionary conserved mechanism by which RNA duplexes, known as short interfering RNA (siRNA), can reduce gene expression through enzymatic cleavage of complementary mRNA. In addition to synthetic siRNA, RNAi can also be induced in mammalian cells by plasmid and viral vector systems that express short hairpin RNAs (shRNAs) that are subsequently processed to siRNAs by the cellular machinery. Consequently, the aim of this investigation was to establish a plasmid DNA vector-based RNAi assay whereby the expression of the AHSV-9 NS2 gene could be suppressed in BHK-21 cells. Complementary oligonucleotides corresponding to selected AHSV-9 NS2 gene sequences were chemically synthesized, annealed and cloned into the pSUPER shRNA delivery vector, downstream of the RNA polymerase III H1 promoter. The vector-expressed shRNAs targeted regions within the NS2 gene corresponding to nucleotides 211 to 230 (shRNA-211), 377 to 396 (shRNA-377) and 958 to 977 (shRNA-958), respectively. To determine whether the NS2-directed shRNAs was capable of silencing NS2 protein expression, BHK-21 cells were co-transfected with the respective pSUPER shRNA delivery vectors and a NS2 reporter plasmid, pCMV-NS2-eGFP. Fluorescence microscopy indicated that NS2-eGFP expression was makerdly reduced in these cells compared to cells transfected with the reporter plasmid only, and fluorometry analysis indicated that the level of inhibition mediated by the shRNAs were in excess of 80%. The potential of the NS2-directed shRNAs to reduce the level of NS2 transcripts in AHSV-9 infected BHK-21 cells was also investigated by transfection of the BHK-21 cells with the respective pSUPER shRNA delivery vectors, followed by virus infection. Results obtained by means of semi-quantitative real-time reverse transcriptase-polymerase chain reactions indicated that shRNA-377 interfered the most efficiently with NS2 mRNA expression, and the greatest silencing effect was observed at 24 h post-infection. During the course of this investigation it was also attempted to establish a BHK-21 cell line that stably expressed the NS2-directed shRNA-377. For this purpose, a recombinant pSUPER.Retro.Puro retroviral vector was constructed and following transfection of BHK-21 cells, stable transfectants were selected by growth in the presence of puromycin. Results indicated that although the derived cell line suppressed AHSV-9 NS2 mRNA expression, the plasmid DNA was maintained extrachromosomally. Overall, the results of this investigation have provided evidence that AHSV-9 NS2 gene expression can be suppressed in mammalian cells by vector-derived shRNAs. / Dissertation (MSc)--University of Pretoria, 2010. / Microbiology and Plant Pathology / unrestricted
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Significance of sequence variation in the P1 and 3A genes of foot-and-mouth-disease virus isolates from southern AfricaHeath, Livio Edward 17 February 2006 (has links)
Please read the abstract in the section 00front of this document / Dissertation (MSc Agric (Microbiology))--University of Pretoria, 2006. / Microbiology and Plant Pathology / unrestricted
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The Effect of Frequency on Visually Induced Motion Sickness (VIMS) through Virtual Reality (VR) StimulusQi, Jiakang January 2017 (has links)
No description available.
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