OBJECTIVES: To compare the amount of fluoride, calcium and phosphate release and recharge of a fluoride containing bioactive glass composite to a conventional resin composite and a resin modified glass ionomer cement at different time points. Furthermore, bond strength of a fluoride containing bioactive glass composite, a conventional flowable composite, and a resin modified glass ionomer cement to metal orthodontic brackets was evaluated. METHODS: A fluoride containing bioactive glass (BG) was synthesized using a sol-gel method and mixed homogeneously with an unfilled resin. For ion release and recharge, resin modified glass ionomer (RMGIC), Photac Fil Quick Aplicap (3M/ESPE) and flowable composite (Control), Filtek Supreme Ultra (Kerr), were used for comparison. Disc shape samples were fabricated using custom aluminum mold (1 mm in thickness and 9 mm in diameter, (n=5 for each material) and stored in 15 mL deionized water at 37°C until the testing time. The amounts of fluoride, calcium, and phosphate ions released were evaluated at different time points: 1 hour, 24 hours, 2 days, 3 days, 4 days, 5 days 6 days and 7 days. At each time point, all of the storage solution was extracted, and 7.5 mL was used for fluoride release measurement and the remaining 7.5 mL for calcium and phosphate ion release measurements. After solution extraction, the samples were replaced in 15 mL fresh deionized water at 37°C until the next sampling time point. Ionic recharge was performed with 5% sodium fluoride varnish (FluoroDose, Centrix) and MI paste plus (GC) following the ion release-testing period. An ion meter with a Fluoride ionic selective electrode were used to determine fluoride concentration. A Microwave-Plasma Atomic Emission Spectrometer (MP-AES) was used to test the concentration of the calcium and phosphate. For the shear bond strength test, rectangular shaped ceramic samples with the dimensions of 2 mm x 12 mm x 14 mm (Vita Mark II, Vita) were fabricated. Standard edgewise-metal brackets (American Orthodontics) were bonded to the center of the ceramic samples using tested material (n=10 for each material). Excess material was removed, and the cementing materials were polymerized from each side for 20 seconds. Specimens were either stored in water for 24 hours at 37o C or went under thermocycling for 5000 cycles. After the storage period, the specimens were subjected to shear bond strength test using an Instron universal machine at a crosshead speed of 0.5mm/min. Loads to failure were recorded to calculate shear bond strength. Comparison of released/recharged ions and shear bond strength were done by ANOVA and Tukey-Kramer HSD (α = 0.05) using JMP Pro 13. RESULTS: RMGIC showed significantly higher fluoride release and recharge than BG composite and the control. BG showed significantly higher Ca and P ion release compared to RMGIC followed by composite. RMGIC and BG showed significant ion recharge capability compared to composite. For the shear bond strength, the control composite showed significantly higher shear bond strength than BG composite followed by RMGIC. Thermocycling significantly increase bond strength for RMGIC and control but not for BG composite. CONCLUSIONS: 1. A fluoride containing bioactive glass composite was fabricated that showed the ability of ion release and recharge. 2. There was a significant difference in the amount of ion release and recharge among tested materials at different time points. 3. Favorable fluoride, calcium and phosphate ion release and recharge of BG composite were maintained over the testing period. 4. BG composite showed favorable bond strength to orthodontic metal brackets. 5. Thermocycling had a significant influence in bond strength for the materials tested except for BG composite. / 2020-07-18T00:00:00Z

<p>A literature survey on modelling of the resistance spot welding process has been carried out and some of the more interesting models on this subject have been reviewed in this work. The underlying physics has been studied and a brief explanation of Heat transfer, electrokinetics and metallurgy in a resistance spot welding context have been presented.\nl\hsLastly a state of the art model and a simplified model, with implementation in the FEM software LS-DYNA in mind, have been presented.</p>

With the increase in atmospheric CO2 concentrations, and the resulting potential for climate change, there has been increasing research devoted to understanding the factors that determine the magnitude of CO2 fluxes and the feedback of ecosystem fluxes on climate. This thesis is an effort to investigate the feasibility of using alternate methods to measure and estimate the CO2 exchange rates in the northern mixed grass prairie. Specifically, the objectives are to evaluate the capability of using ground-level hyperspectral, and satellite-level multispectral data in the estimation of mid-season leaf CO2 exchange rates as measured with a chamber, in and around Grasslands National Park (GNP), Saskatchewan. Data for the first manuscript was collected during June of 2004 (the approximate period for peak greenness for the study area). Spectral reflectance and CO2 exchange measurements were collected from 13 sites in and around GNP. Linear regression showed that the Photochemical Reflectance Index (PRI) calculated from hyperspectral ground-level data explained 46% of the variance seen in the CO2 exchange rates. This indicates that the PRI, which has traditionally been used only in laboratory conditions to predict CO2 exchange, can also be applied at the canopy level in grassland field conditions. <p>The focus of the second manuscript is to establish if the relationship found between ground-level hyperspectral data and leaf CO2 exchange is applicable to satellite-level derived vegetation indices. During June of 2005, biophysical and CO2 exchange measurements were collected from 24 sites in and around GNP. A SPOT satellite image was obtained from June 22, midway through the field data collection. Cubic regression showed that Normalized Difference Vegetation Index (NDVI) explained 46% of the variance observed in the CO2 exchange rates. To our knowledge, this is the first time that a direct correlation between satellite images and leaf CO2 fluxes has been shown within the grassland biome.

In order to meet the increasing fuel efficiency requirements, the automotive industry has strived for component weight reduction in order to improve the performance of automotive vehicles through the use of light Al and Mg alloys. Resistance spot welding (RSW) currently is the primary joining method in the manufacturing of automotive assemblies. With the increased use of Al and Mg, there is a pressing need for a technology to produce dissimilar Al/Mg joints, and preferably by RSW since this technology is already prevalent in the industry. Direct welding of Al to Mg usually results in formation of hard and brittle intermetallic compounds and poor quality of the welds. Employing an interlayer is a promising approach to overcome this problem. Current literature, however, does not consider the effects of different interlayers on RSW of Al to Mg. This thesis examines effects of different interlayers on microstructure and mechanical properties of Al/Mg joints made by RSW. Effects of three types of interlayers, specifically pure Ni foil, Au-coated Ni foil and Zn-coated were investigated in details. While only brief investigation of joints made with Sn-coated steel, Zn foil, and Cu foil interlayers was conducted. No joints were achieved with a bare Ni interlayer during Al to Mg alloy resistance spot welding, as coupons separated without applying any force. The Ni interlayer remained intact and Al-Mg intermetallic compounds did not form. Addition of Au coating on Ni surface greatly contributed to the metallurgical bonding at the interfaces and welds easily met requirements of the AWS D17.2 standard. Average lap-shear strength reached 90% of that in similar AZ31B resistance spot welds. Acceptable welds were also produced using galvanised Zn-coated steel interlayer, which easily met strength requirements of the AWS D17.2 standard. Average failure load reached 74% of same size similar AZ31B joints. The steel interlayer was not melted which prevented mixing of Al and Mg. The Zn coating on the steel interlayer was melted and squeezed to the nugget periphery, providing a clean steel surface for welding-brazing in the center and acting as a solder metal at the periphery. A feasibility study of Al/Mg RSW with Sn-coated steel, Zn foil and Cu foil interlayers was also conducted. Mechanical properties of welds made with Sn-coated steel interlayer were very similar to those made with Zn-coated steel interlayer. While welds made with only a Zn foil interlayer were much weaker. The Zn foil completely melted during the welding which resulted in formation of brittle Al-Mg-Zn phases. None of the welds made with Zn foil interlayer met requirements of the AWS D17.2 standard. RSW of Al to Mg with Cu foil interlayer also could not produce welds with acceptable strength.

The automotive industry is adopting lightweight materials to improve emissions and fuel economy. Magnesium (Mg) alloys are the lightest of engineering metals, but work is required to assess their structural strength, especially for spot-welded applications. In the present research, fatigue behavior of magnesium spot-welds was characterized and compared with steel and aluminum spot-welds. A fatigue model was proposed to predict the failure location and crack initiation life in magnesium structures. The material under investigation, AZ31B-H24 Mg alloy, and its spot-welds were characterized from microstructural and mechanical perspectives. Microstructure and hardness of the base metal (BM) and different regions in the spot-welds were studied. Under cyclic loading, the BM had an asymmetric hysteresis loop. Cyclic behavior of magnesium spot-welds was measured using different specimen configurations, and the effect of geometrical factors on fatigue life was evaluated. A constitutive model was developed to model the asymmetric hardening behavior of wrought magnesium alloys under cyclic loading. An algorithm for numerical implementation of the proposed model was developed. The numerical formulation was programmed into a user material subroutine to run with the commercial finite element software Abaqus/Standard. The proposed model was verified by solving two problems with available solutions. A number of available fatigue models, as well as a new model proposed in this research were assessed by predicting fatigue life of magnesium spot-welds. The new model used a strain energy damage parameter. All models were evaluated by comparing the predicted and experimental fatigue lives for different Mg spot-welded specimens. The effect of considering the asymmetric hardening behavior of wrought magnesium alloys on the accuracy of the fatigue life prediction was not significant for the available experimental data. This was attributed to the limited experimental data on spot-welded specimens. The proposed material model and fatigue damage parameter were verified by simulating a real-life structure manufactured and fatigue tested by the US Automotive Materials Partnership. The results obtained from the proposed asymmetric model were compared with available symmetric simulation results and experimental data. The asymmetric material model along with the proposed damage parameter resulted in more accurate prediction of fatigue failure location and life.

Various environmental standards have been established for the sake of public health and ecosystem diversity since environmental awareness was awakened in the late 1960s. However, the results were often unsatisfactory. Either environmental goals achieved were far from desired, or regional development was hampered due to some unpractical high environmental standards. The failure of these environmental standards resulted in innovations of environmental policy instruments to find practical environmental goals and methods approaching them scientifically. Another class of environmental policy instruments, so called economic incentive policies, is established based on environmental economics theory. A neo-classical economics framework is founded for setting appropriate environmental goals and assessing efficiency of environmental policies in reaching these goals. This thesis summarizes rationales and factors affecting the performance for environmental policy instruments under the neo-classical economic framework. Since the acid rain program, the first large-scale implementation of the emissions trading policy, has achieved great success in reducing SO&#8322; emissions from the electricity generators in the United States, the emission trading policy attracted many interests in this kind of environmental policy instrument. Many countries, such as China, plan to adopt the emissions trading policy to address various environmental problems. Hence, factors leading to the success of this program should be identified. Potential risks and problems must be addressed as well lest the emissions trading policy causes some problem during implementation. Feasibility of implementing an emissions trading policy will be discussed based on these results. Three kinds of geographic analyses, change detection, network analysis, and hot spots identification, are conducted in this thesis to study the effectiveness and efficiency of the acid rain program. It is found that the acid rain program is successful in improving the sustainability of the economic development in the United States. But the effectiveness is not as great as the high emissions cutting rate achieved in this program. In addition, the acid rain program lowers the compliance costs of achieving the environmental goal since the radius of the high quality coal service area doubles. Lastly, hot spots are found around the Ohio River valley and Los Angeles. Suggestions on integrating geographic factors into the economic framework are presented in order to eliminate the risk of causing severe environmental problems. Finally, the feasibility of migrating the emissions trading policy to China is discussed. Further work can be conducted in this direction to realize sustainable development quicker with lower costs.

The influence of the tribology during resistance spot welding (RSW) of aluminum alloy 5182 with spherical-tip electrode has been investigated at both the electrode-worksheet (E/W) and faying surface (FS) interfaces. In RSW, electrode life is limited by poor current transport to the FS interface caused by extensive pitting of the electrode tip surface. The primary focus of the present research was to extend electrode life by using the knowledge gained from studying the contact mechanics at both of these interfaces. Series of experiments were conducted and finite element analysis was employed to investigate the contact mechanics at the interfaces. Based on these findings, a practical way to extend the electrode life was developed. In a series of initial experiments, it was found that attempts to alter the worksheet surface roughness caused damage to the surface oxide layer which resulted in decrease of electrical contact resistance at the E/W interface. The oxide layer on the worksheet surface contained aluminum and magnesium oxide regions and abrasion of the worksheet surface reduced the oxide layer thickness and made it more uniform in composition because when the magnesium oxide regions were abraded, a thin layer of aluminum oxide re-formed immediately while it take specific conditions to re-form magnesium oxide. These factors decreased the electrical contact resistance of the E/W interface compared with the as-received surface, thus reducing heat generation and the associated pitting of the electrode surface during RSW. Further experimental investigations and finite element analysis showed that the contact mechanics that occurred during the loaded “squeezing” phase of the welding sequence, but before current was applied to cause RSW, had a significant effect on the electrode pitting behaviour and nugget formation. At the E/W interface, squeezing caused high shear stress and slip at the periphery of the contact region. This slip disrupted the oxide layer and reduced the electrical resistance. At the beginning of the current phase of the weld sequence, the reduced electrical resistance caused current to concentrate near the periphery but constriction resistance still produced enough heat generation to cause alloying, pickup and eventually pitting of electrode in a ring around the contact centre. At the FS interface, experiments and finite element analysis showed that sheet separation and thus bending occurred during the squeezing phase and this had a profound influence on nugget formation. Experimental observations showed that the bending caused enlarged and aligned cracks in the surface oxide layers which promoted good metal-to-metal contact near the periphery of the FS. As at the E/W interface, high current densities occurred at the beginning of the current phase and the constriction resistance caused significant heat generation in this zone due to an increasing constriction resistance. Consequently, the melting at the FS started near the periphery and moved in towards the central zone of the contact region melted to produce a “doughnut-shaped” nugget with a filled-in but thin central region. Low electrical contact resistance at the E/W interface led to longer electrode tip life because less pitting occurred. In addition, higher current densities could then develop at the FS to affect RSW and achieve good nugget formation despite the rather uneven peripheral heat generation. In attempts to reduce the electrical resistance at the E/W interface, several boundary lubricants were placed on the worksheet surface a short time before starting RSW and they altered the tribology. Both increased and decreased electrode degradation rate were found in electrode life tests. One lubricant was found to be particularly effective in lowering the electrode pitting rate. It extended the electrode life to almost double that occurring with as-received (unlubricated) surfaces. Detailed analysis revealed that the effective boundary lubricant had a beneficial chemical influence on the surface of the AA5182 worksheet. The lubricant chemically attacked the oxide layer thus reducing its thickness and reducing electrical contact resistance of the E/W interface at the critical peripheral region. The result was a lower electrode pitting rate and an extended electrode life. The improved understanding of the current flow during the critical initial period and its dependence on the contact mechanics of the E/W and FS interfaces was considered important in developing ways of improving weld strength and increasing electrode life. The finding of a boundary lubricant that acted to reduce oxide layer thickness was considered an important starting point for industrial development of RSW with longer electrode life. It could be employed without interrupting the RSW process and its efficacy was well-supported by the present contact mechanics studies in which the key role of the oxide layer was demonstrated.

EVALUATION AND DETERMINATION OF THE SENSITIVITY AND SPECIFICITY OF A Treponema pallidum DRIED BLOOD SPOT (DBS) METHOD FOR SEROLOGIC DIAGNOSIS OF SYPHILIS Background: Syphilis is a sexually transmitted infection (STI) caused by Treponema pallidum subspecies pallidum. Syphilis is known as the "great imitator" due to the similarity of clinical signs and symptoms to other infectious diseases. The primary diagnosis of syphilis relies on clinical findings, including the examination of treponemal lesions, and/or serologic tests. Serologic tests are divided into nontreponemal and treponemal tests. Nontreponemal tests are useful for screening, while treponemal tests are used as confirmatory tests. Methods: A total of 200 serum and DBS specimens collected from patients at the Los Angeles Municipal Sexually Transmitted Disease Clinics were tested by the DBS and enzyme immunoassay (EIA) methods. These samples were sent to the Syphilis Diagnostics Laboratory, Centers for Disease Control and Prevention (CDC) in Atlanta, Georgia for testing. Samples were blindly evaluated by the TREP-SPOTTM DBS and the TREP- SURETM EIA methods for the detection of anti-treponemal IgG- and IgM-class antibodies. Results: The sensitivity of the DBS method was 83% (95% CI, 73.89 - 89.50) and specificity was 100% (95% CI, 95.39 - 100)). The positive predictive value and negative predictive values were 100% (95% CI, 94.48 - 100) and 85% (95% CI, 77.43 - 91.0), respectively. The efficiency of the DBS method was 91.5%. The kappa value for the agreement between the DBS method and EIA assay was 0.83 (95% CI, 0.754 - 0.906). The correlation coefficient (r2) between the anti-treponemal antibody assay results obtained from DBS and serum samples was 0.94. Conclusion: DBS is an optimal choice to be used as a screening tool for the detection of anti-treponemal antibodies for the diagnosis of syphilis. The detection of anti-treponemal antibodies (TREP-SPOTTM DBS EIA) compared favorably to the results of serum-base assay (TREP-SURETM EIA), with an overall concordance of 91.5%. Dried blood spots are technically easier to obtain and are suitable blood samples for primary health care centers.

Various environmental standards have been established for the sake of public health and ecosystem diversity since environmental awareness was awakened in the late 1960s. However, the results were often unsatisfactory. Either environmental goals achieved were far from desired, or regional development was hampered due to some unpractical high environmental standards. The failure of these environmental standards resulted in innovations of environmental policy instruments to find practical environmental goals and methods approaching them scientifically. Another class of environmental policy instruments, so called economic incentive policies, is established based on environmental economics theory. A neo-classical economics framework is founded for setting appropriate environmental goals and assessing efficiency of environmental policies in reaching these goals. This thesis summarizes rationales and factors affecting the performance for environmental policy instruments under the neo-classical economic framework. Since the acid rain program, the first large-scale implementation of the emissions trading policy, has achieved great success in reducing SO&#8322; emissions from the electricity generators in the United States, the emission trading policy attracted many interests in this kind of environmental policy instrument. Many countries, such as China, plan to adopt the emissions trading policy to address various environmental problems. Hence, factors leading to the success of this program should be identified. Potential risks and problems must be addressed as well lest the emissions trading policy causes some problem during implementation. Feasibility of implementing an emissions trading policy will be discussed based on these results. Three kinds of geographic analyses, change detection, network analysis, and hot spots identification, are conducted in this thesis to study the effectiveness and efficiency of the acid rain program. It is found that the acid rain program is successful in improving the sustainability of the economic development in the United States. But the effectiveness is not as great as the high emissions cutting rate achieved in this program. In addition, the acid rain program lowers the compliance costs of achieving the environmental goal since the radius of the high quality coal service area doubles. Lastly, hot spots are found around the Ohio River valley and Los Angeles. Suggestions on integrating geographic factors into the economic framework are presented in order to eliminate the risk of causing severe environmental problems. Finally, the feasibility of migrating the emissions trading policy to China is discussed. Further work can be conducted in this direction to realize sustainable development quicker with lower costs.

The influence of the tribology during resistance spot welding (RSW) of aluminum alloy 5182 with spherical-tip electrode has been investigated at both the electrode-worksheet (E/W) and faying surface (FS) interfaces. In RSW, electrode life is limited by poor current transport to the FS interface caused by extensive pitting of the electrode tip surface. The primary focus of the present research was to extend electrode life by using the knowledge gained from studying the contact mechanics at both of these interfaces. Series of experiments were conducted and finite element analysis was employed to investigate the contact mechanics at the interfaces. Based on these findings, a practical way to extend the electrode life was developed. In a series of initial experiments, it was found that attempts to alter the worksheet surface roughness caused damage to the surface oxide layer which resulted in decrease of electrical contact resistance at the E/W interface. The oxide layer on the worksheet surface contained aluminum and magnesium oxide regions and abrasion of the worksheet surface reduced the oxide layer thickness and made it more uniform in composition because when the magnesium oxide regions were abraded, a thin layer of aluminum oxide re-formed immediately while it take specific conditions to re-form magnesium oxide. These factors decreased the electrical contact resistance of the E/W interface compared with the as-received surface, thus reducing heat generation and the associated pitting of the electrode surface during RSW. Further experimental investigations and finite element analysis showed that the contact mechanics that occurred during the loaded “squeezing” phase of the welding sequence, but before current was applied to cause RSW, had a significant effect on the electrode pitting behaviour and nugget formation. At the E/W interface, squeezing caused high shear stress and slip at the periphery of the contact region. This slip disrupted the oxide layer and reduced the electrical resistance. At the beginning of the current phase of the weld sequence, the reduced electrical resistance caused current to concentrate near the periphery but constriction resistance still produced enough heat generation to cause alloying, pickup and eventually pitting of electrode in a ring around the contact centre. At the FS interface, experiments and finite element analysis showed that sheet separation and thus bending occurred during the squeezing phase and this had a profound influence on nugget formation. Experimental observations showed that the bending caused enlarged and aligned cracks in the surface oxide layers which promoted good metal-to-metal contact near the periphery of the FS. As at the E/W interface, high current densities occurred at the beginning of the current phase and the constriction resistance caused significant heat generation in this zone due to an increasing constriction resistance. Consequently, the melting at the FS started near the periphery and moved in towards the central zone of the contact region melted to produce a “doughnut-shaped” nugget with a filled-in but thin central region. Low electrical contact resistance at the E/W interface led to longer electrode tip life because less pitting occurred. In addition, higher current densities could then develop at the FS to affect RSW and achieve good nugget formation despite the rather uneven peripheral heat generation. In attempts to reduce the electrical resistance at the E/W interface, several boundary lubricants were placed on the worksheet surface a short time before starting RSW and they altered the tribology. Both increased and decreased electrode degradation rate were found in electrode life tests. One lubricant was found to be particularly effective in lowering the electrode pitting rate. It extended the electrode life to almost double that occurring with as-received (unlubricated) surfaces. Detailed analysis revealed that the effective boundary lubricant had a beneficial chemical influence on the surface of the AA5182 worksheet. The lubricant chemically attacked the oxide layer thus reducing its thickness and reducing electrical contact resistance of the E/W interface at the critical peripheral region. The result was a lower electrode pitting rate and an extended electrode life. The improved understanding of the current flow during the critical initial period and its dependence on the contact mechanics of the E/W and FS interfaces was considered important in developing ways of improving weld strength and increasing electrode life. The finding of a boundary lubricant that acted to reduce oxide layer thickness was considered an important starting point for industrial development of RSW with longer electrode life. It could be employed without interrupting the RSW process and its efficacy was well-supported by the present contact mechanics studies in which the key role of the oxide layer was demonstrated.