<p>The use of insecure networks -such as the Internet- to send and receive information has made the need for preventing unauthorised people reading it yet more important. One of the easiest way to do this is through public key cryptography. However, the problem with this solution is how to tie a specific public key to a certain subject. This is solved by letting a trusted third party issue a certificate that holds, as a minimum, the name of the subject and the subject's public key along with the issuer's digital signature on the information. The rules we make for issuing, revoking and verifying of certificates and the entities that are being used to do so are called PKI - Public Key Infrastructure. In this thesis we shall se what PKI really is in a more detailed way and which entities it constitutes of. We will also investigate some of the areas in which we could make use of it, for instance secure e-mail and virtual private networks. Next, we will look into some of the drawbacks with PKI and what you should think of in order to aviod these. Finally, we'll give recommendations for the implementation itself. As for the theory of cryptography, the basics is presented to the interested reader in a separate appendix.</p>

The use of insecure networks -such as the Internet- to send and receive information has made the need for preventing unauthorised people reading it yet more important. One of the easiest way to do this is through public key cryptography. However, the problem with this solution is how to tie a specific public key to a certain subject. This is solved by letting a trusted third party issue a certificate that holds, as a minimum, the name of the subject and the subject's public key along with the issuer's digital signature on the information. The rules we make for issuing, revoking and verifying of certificates and the entities that are being used to do so are called PKI - Public Key Infrastructure. In this thesis we shall se what PKI really is in a more detailed way and which entities it constitutes of. We will also investigate some of the areas in which we could make use of it, for instance secure e-mail and virtual private networks. Next, we will look into some of the drawbacks with PKI and what you should think of in order to aviod these. Finally, we'll give recommendations for the implementation itself. As for the theory of cryptography, the basics is presented to the interested reader in a separate appendix.

Submitted by Renata Lopes (renatasil82@gmail.com) on 2015-12-07T11:09:22Z No. of bitstreams: 1 nicoleandradedarocha.pdf: 5589274 bytes, checksum: e35a9c2511e226e70b29092b3dd7f8b9 (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2015-12-07T21:39:14Z (GMT) No. of bitstreams: 1 nicoleandradedarocha.pdf: 5589274 bytes, checksum: e35a9c2511e226e70b29092b3dd7f8b9 (MD5) / Made available in DSpace on 2015-12-07T21:39:14Z (GMT). No. of bitstreams: 1 nicoleandradedarocha.pdf: 5589274 bytes, checksum: e35a9c2511e226e70b29092b3dd7f8b9 (MD5) Previous issue date: 2015-02-25 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Este trabalho tem por objetivo principal testar a ferramenta OSS (Observação Social Sistemática) e suas possíveis contribuições para a arquitetura e urbanismo, de forma a aprofundar nos estudos análiticos da realidade urbana e arquitetônica brasileira, visando uma leitura adequada do espaço urbano, tanto físico, quanto formal, que permita compreender o espaço enquanto lugar e as relações que são desenvolvidas nesse contexto. Para tanto, o estudo foi aplicado em duas Regiões Urbanas (RU) de Juiz de Fora- MG, dividido em quatro etapas de estudos: na etapa quali-quantitativa (1), foi realizada uma busca sistemática no Periódicos Capes através de palavras-chave relacionados com o estudo em questão, buscando desta forma, conhecer o estado da arte, métodos mais utilizados e o contexto de aplicação das ferramentas analisadas, que possibilitassem as realizações das análises qualitativa do espaço urbano; na etapa prática (2), aplicou-se a ferramenta nas áreas selecionadas e após a compilações dos dados, expôs-se os resultados encontrados; na etapa de discussão dos resultado (3), buscou-se identificar a aplicabilidade e as limitações do uso da ferramenta; e na etapa final, realizou-se as conclusões e considerações finais (4). Entre os resultados encontrados, conclui-se que a flexibilidade destaca-se entre as vantagens da OSS, já que é possível adaptá-la há vários objetos de estudos e as várias áreas do conhecimento, uma vez que utiliza o levantamento quantitativo dos elementos da paisagem permitindo concomitantemente analises qualitativa da mesma, oportunizando o direcionamento da pesquisa de acordo com enfoco da área estudada. Entretanto, a ferramenta possibilita a análise apenas da camada mais próxima da paisagem, sem explorá-la mais profundamente e em detalhes já que atua na escala do pedestre. Além disso, a OSS se mostra interessante ao propor um processo rápido e barato da captação das camadas da paisagem mais próxima do observador. Desta forma, quando buscamos respostas, na arquitetura e urbanismo, para as questões que envolvem métricas do espaço urbano a partir de estudos sistemáticos, qualitativos e quantitativos, percebemos que existem poucos estudos desenvolvidos nesse sentido. Talvez, uma alternativa a essa questão seja o de buscar, em outras áreas do conhecimento (que é multidisciplinar), a contribuição e apoio pautados nos princípios heurísticos permitindo novos rumos para a pesquisa. / This work has as main objective to test the OSS tool (Social Systematic Observation) and their contributions to the study of architecture and urbanism, in order to deepen the analytical studies of urban reality and Brazilian architectural, seeking a proper reading of urban space, both physical, as formal, in order to understand the space as a place and the relationships that are developed in this context. Therefore, the study was applied in two Urban Regions (RU) in Juiz de Fora - MG, the study was divided into four stages: Qualitative and quantitative stage (1), was a systematic search in Portal Periodicos Capes through keywords related to the study in question, looking this way, knowing the state of the art, methods more used and the application context of the analyzed tools that would enable the achievements of qualitative analysis of urban space; Practice stage (2) in which the tool was applied in selected areas and after compilation of data and was explained the results; Discussion of the result (3), we sought to identify the applicability and limitations of the tool; and in the last stage the final conclusions (4). Among the results, we can conclude that flexibility stands out like advantages of OSS, because it is possible to adapt it for several objects of study and the various areas of knowledge, since it uses the quantitative survey of landscape elements concurrently allowing qualitative analysis thereof. However the tool allows the analysis only in the layer closest to the landscape without exploring it more deeply and in detail. This tool shows interesting on to propose a quick and inexpensive process of capturing the landscape layers in the pedestrian scale, even if it is the closest to the observer. Thus, when we seek answers, in architecture and urbanism, to the issues surrounding metrics of urban space from systematic, qualitative and quantitative studies, we realize that there are few studies in this direction. Perhaps an alternative to this question is to seek, in other areas of knowledge that is multidisciplinary, the contribution of knowledge, support and insights for new directions of research.

<p>Genetic profiling is commonly performed on the autosomes using multiple DNA markers. Although routine forensic DNA analysis is robust and based on reliable technologies, samples with degraded or limited amounts of DNA often fail. In these cases, the analysis of mitochondrial DNA (mtDNA) can be very valuable due to the high copy number per cell. This thesis describes evaluation and modifications of existing technologies that are useful in forensic DNA typing, mainly focusing on mtDNA.</p><p>DNA quantities isolated from common evidence materials such as hairs, fingerprints and accessories were estimated using a real-time quantification assay. Knowledge of quantitative differences between materials can guide forensic scientists to perform the best analysis (Paper I).</p><p>The current mtDNA analysis is based on hypervariable region (HVI/HVII) sequencing, which is the most rigorous and time-consuming forensic DNA analysis. Therefore, we evaluated the possibility to exclude individuals by screening for non-matching samples using the rapid and easy mtDNA Linear Array Assay (Paper II). </p><p>The major disadvantage using mtDNA is the lower discrimination power compared to multiple nuclear DNA markers. In contrast to the nuclear genome, due to the uniparental (maternal) mode of inheritance, no individual has unique mtDNA. We investigated the possibility of increasing the discrimination power by using pyrosequencing technology to analyse parts of the coding region in addition to HVI/HVII (Paper III). Furthermore, the addition of coding mtDNA information was evaluated by comparing several recently published mtDNA coding region assays (Paper IV). </p><p>Mixtures of DNA are common in forensic genetics due to contribution of DNA from several individuals, contamination or heteroplasmy. To resolve mixtures we have developed a pyrosequencing-based assay for the accurate quantification of the mtDNA mixture components (Paper V).</p><p>In conclusion, this thesis describes several assays that are valuable in forensic genetics for DNA quantification, improved mtDNA analysis, and mtDNA mixture interpretation.</p>

Genetic profiling is commonly performed on the autosomes using multiple DNA markers. Although routine forensic DNA analysis is robust and based on reliable technologies, samples with degraded or limited amounts of DNA often fail. In these cases, the analysis of mitochondrial DNA (mtDNA) can be very valuable due to the high copy number per cell. This thesis describes evaluation and modifications of existing technologies that are useful in forensic DNA typing, mainly focusing on mtDNA. DNA quantities isolated from common evidence materials such as hairs, fingerprints and accessories were estimated using a real-time quantification assay. Knowledge of quantitative differences between materials can guide forensic scientists to perform the best analysis (Paper I). The current mtDNA analysis is based on hypervariable region (HVI/HVII) sequencing, which is the most rigorous and time-consuming forensic DNA analysis. Therefore, we evaluated the possibility to exclude individuals by screening for non-matching samples using the rapid and easy mtDNA Linear Array Assay (Paper II). The major disadvantage using mtDNA is the lower discrimination power compared to multiple nuclear DNA markers. In contrast to the nuclear genome, due to the uniparental (maternal) mode of inheritance, no individual has unique mtDNA. We investigated the possibility of increasing the discrimination power by using pyrosequencing technology to analyse parts of the coding region in addition to HVI/HVII (Paper III). Furthermore, the addition of coding mtDNA information was evaluated by comparing several recently published mtDNA coding region assays (Paper IV). Mixtures of DNA are common in forensic genetics due to contribution of DNA from several individuals, contamination or heteroplasmy. To resolve mixtures we have developed a pyrosequencing-based assay for the accurate quantification of the mtDNA mixture components (Paper V). In conclusion, this thesis describes several assays that are valuable in forensic genetics for DNA quantification, improved mtDNA analysis, and mtDNA mixture interpretation.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-01-23T14:13:10Z No. of bitstreams: 1 PhelipeSenaOliveira_TESE.pdf: 2369727 bytes, checksum: 2bb1dad07260b4efd645c7028636e62f (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-01-24T15:05:44Z (GMT) No. of bitstreams: 1 PhelipeSenaOliveira_TESE.pdf: 2369727 bytes, checksum: 2bb1dad07260b4efd645c7028636e62f (MD5) / Made available in DSpace on 2018-01-24T15:05:44Z (GMT). No. of bitstreams: 1 PhelipeSenaOliveira_TESE.pdf: 2369727 bytes, checksum: 2bb1dad07260b4efd645c7028636e62f (MD5) Previous issue date: 2017-02-06 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / As novas configura??es de dispositivos e circuitos de micro-ondas resulta em aumento de complexidade nos processos de desenvolvimento, particularmente nos casos de s?ntese. Antenas e superf?cies seletivas de frequ?ncia (Frequency Selective Surface - FSS) s?o estruturas muito importantes nos sistemas de comunica??es, que podem ser otimizadas pela atua??o dos algoritmos bioinspirados. Geralmente, para que os algoritmos bioinspirados auxiliem na otimiza??o dessas estruturas ? necess?ria a implementa??o de t?cnicas de an?lise num?rica como o M?todo dos Momentos ou Elementos Finitos. Diante da dificuldade de modelagem das t?cnicas descritas, ? comum a utiliza??o de uma fun??o de estima??o, como as Redes Neurais Artificiais, para substituir a an?lise num?rica. Embora encontre solu??es desejadas, as estima??es s?o realizadas ap?s um processo de treinamento efetuado atrav?s de uma base de dados. Cada estrutura de antena ou FSS exige uma base de dados espec?fica. Este trabalho prop?e uma t?cnica de otimiza??o baseado na utiliza??o de um algoritmo bioinspirado e um framework que realiza a integra??o do algoritmo ao software comercial ANSYS HFSS. O prop?sito ? realizar as simula??es das poss?veis solu??es durante a execu??o do algoritmo bioinspirado, e com isso, evitar o uso de uma base de dados, e assim, tornar a t?cnica eficiente e flex?vel com rela??o ?s altera??es nas estruturas de antenas ou FSS. Outro objetivo original deste trabalho ? a utiliza??o do algoritmo Social Spider Optimization ? SSO para a solu??o de problemas na ?rea de eletromagnetismo aplicado. Para comprovar a efic?cia da t?cnica proposta alguns projetos de antena e FSS s?o otimizados atrav?s de varia??es em seus par?metros estruturais. Os resultados obtidos na otimiza??o dos projetos dos circuitos s?o comparados com outras t?cnicas, tendo sido observado uma boa concord?ncia. / The new settings for devices and microwaves circuits result in an increase of complexity in the processes of development, specially in regards to the process of synthesis. Antennas and Frequency Selective Surfaces (FSS) are primordial in communication systems. Alternatively, these structures may be optimized through the usage of bio-inspired algorithms. Generally, in order for the bio-inspired algorithms to assist in the optimization of FSS structures, it is necessary to implement several numerical analysis techniques, such as the method of moments or finite elements. Because of the difficulty to model such methods, it has been turned common the usage of a point estimation function, such as artificial neural networks, to substitute the numerical analysis. Although these procedures end up encountering desirable solutions, the estimations are done after a training process conducted through a database. Additionally, every FSS antenna demands a specific database. This work proposes an optimization technique that relies on the usage of a bio-inspired algorithm and a framework (that provides the integration between the algorithm and the commercial software ANSS HFSS). The purpose of the work is to do simulations of possible solutions during the execution of the bio-inspired algorithm; therefore, avoiding the usage of the database; and so, make the technique flexible and efficient in relation to altering the antennas? structures or FSS. Furthermore, another objective of this work is the usage of the algorithm, namely Social Spider Optimization - SSO in the search for solutions in the field of applied electromagnetism. In order to prove the efficiency of the proposed techniques, some FSS? and antennas? projects are optimized through the variation of their structural parameters. In conclusion, the results that were obtained through the procedures previously shown, are compared to other techniques, resulting in a satisfying agreement rate.

This work introduces the available authorization frameworks for the purpose of Single Sign-On functionality within an enterprise, along with the fundamental technicalities. The focus of the work is on SAML 2.0 and OAuth 2.0 frame- works. Following the details related to available protocol flows, supported client profiles and security considerations, the two frameworks are compared in accordance with a set of factors given in a criteria. The report discusses the possibilities provided by a Microsoft Windows based infrastructure, as well as different scenarios and their feasibility in an enterprise environment. The preferred framework, OAuth 2.0, is selected according to the given criteria and the comparative discussions.

No description available.