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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
801

Automatické rozpoznávání registračních značek aut z málo kvalitních videosekvencí / Automated number plate recognition from low quality video-sequences

Vašek, Vojtěch January 2018 (has links)
The commercially used automated number plate recognition (ANPR) sys- tems constitute a mature technology which relies on dedicated industrial cam- eras capable of capturing high-quality still images. In contrast, the problem of ANPR from low-quality video sequences has been so far severely under- explored. This thesis proposes a trainable convolutional neural network (CNN) with a novel architecture which can efficiently recognize number plates from low-quality videos of arbitrary length. The proposed network is experimentally shown to outperform several existing approaches dealing with video-sequences, state-of-the-art commercial ANPR system as well as the human ability to recog- nize number plates from low-resolution images. The second contribution of the thesis is a semi-automatic pipeline which was used to create a novel database containing annotated sequences of challenging low-resolution number plate im- ages. The third contribution is a novel CNN based generator of super-resolution number plate images. The generator translates the input low-resolution image into its high-quality counterpart which preserves the structure of the input and depicts the same string which was previously predicted from a video-sequence. 1
802

Análise da máquina Torus sob frenagem eletrodinâmica

Osório, Jonas Obert Martins January 2011 (has links)
Este trabalho foi desenvolvido com o objetivo de estudar a aplicação, para sistema de frenagem veicular, de uma máquina elétrica sem escovas, de armadura toroidal, e fluxo magnético axial produzido por ímãs permanentes de terras raras, a chamada máquina Torus. A máquina foi construída no LMEAE e estudada inicialmente como motor em outro trabalho. Mas, para que se possa avaliar seu funcionamento em sistema de frenagem, o foco é do ponto de vista da máquina como gerador. São realizados testes dinâmicos e estáticos experimentalmente e modelo numérico pelo método dos elementos finitos com um formato de ímãs permanentes de seção setorial, possibilitando o comparativo com a versão anterior da máquina que empregou ímãs de seção quadrada. Mudanças físicas e no sistema de acionamento da máquina, e ensaios de frenagem dinâmica foram realizados. Modelagem analítica para indução magnética foi desenvolvida utilizando-se da técnica de Transformação Conforme. O trabalho busca apresentar as características da máquina e justificativas que demonstram o seu potencial de aplicabilidade em um subsistema veicular sob frenagem regenerativa e a capacidade de fornecimento de energia a um sistema de armazenamento com uma parte de energia cinética, ou seja, baterias e supercapacitores. / This work is carried out with the aim to study the application, by a vehicular braking system, of a brushless electrical machine with a toroidal armature core, and axial magnetic flux delivered by rare earth permanent magnets, the so-called Torus machine. The machine was built in the LMEAE, and previously studied as a motor by other work. However, in order to assess its performance in a braking system, the focus is the point of view of the machine as a generator. Static and dynamic tests are implemented as well as a numerical model by means of the finite element method, in order to compare the machine with sector poles permanent magnets and with square magnet poles. Physical changes and on the driving system of the machine, and dynamic braking tests are performed. The analytical modelling for the magnetic induction was developed using the technique of conformal transformation. The study aims to present the features of the machine and demonstrates its potential applicability to a vehicular subsystem under regenerative braking and the ability to supply an energy storage system with part of the kinetic energy, i.e. batteries and super capacitors.
803

Propriedades físicas do SnO2: defeitos, impurezas, ligas e superredes. / Physical properties of SnO2: defects, impurities, alloys and superlattices.

Pablo Damasceno Borges 19 August 2011 (has links)
O dioxido de estanho na estrutura rutila (SnO2) é um semicondutor de gap largo e faz parte da classe dos óxidos condutores transparentes (TCO). Possui gap direto de 3,6 eV e condutividade do tipo n, mesmo quando não dopado intencionalmente. Estudos teóricos e experimentais atribuem este comportamento à presença de defeitos intrínsecos. Por outro lado, impurezas de hidrogênio, em sítios intersticiais ou substituindo o átomo de oxigênio, poderiam ser responsáveis pelo caráter n do SnO2. Neste trabalho apresentamos nossos resultados de estrutura eletrônica, a partir de cálculos de primeiros princípios, para o dióxido de estanho puro, assim como levando em conta a presença de defeitos intrínsecos - VO, VSn, Sni, Oi, OSn, SnO, SnO+OSn, Sni+VO - e para vários centros de impureza de hidrogênio - Hi, HO, HBC, Hi-Hi, Hi-HO, Hi-HBC, HBC-HBC, onde V significa vacância e BC a impureza localizada em um sítio entre ligação. Os resultados para a impureza de hidrogênio são confrontados com os dos defeitos intrínsicos. Nossas análises mostram, tanto para o caso das impurezas de H isoladas quanto para os pares complexos H-H, que estes centros apresentam caráter doador. Em todas as configurações, as energias de formação são suficientemente baixas, comparadas com as dos defeitos intrínsecos, mostrando competitividade e sugerindo que a impureza de hidrogênio poderia ser responsável pela característica de condutividade n do cristal SnO2. Apresentamos também resultados de propriedades eletrônicas e magnéticas para impurezas de metal de transição MT (MT = V, Cr, Fe, Mn, Co e Ni) em SnO2 em uma configuração estrutural de baixa concentração. Estes sistemas são denominados semicondutores magnéticos diluídos (DMS - diluted magnetic semiconductor), isto é, ligas diluídas do tipo Sn1-xMTxO2 e Sn1-xMTxO2-y(VO)y. Consideramos neste estudo as concentrações x = 0,04 e y = 0,02, correspondendo a valores experimentalmente possíveis de se obter. Este estudo aponta para a existência de estados magnéticos metaestáveis para estes sistemas e mostra como a vacância de oxigênio afeta este comportamento. Para todos os casos, o estado eletrônico fundamental encontrado apresenta configuraçãoo de alto spin (HS - high-spin) e o fenômeno de spin-crossover para o estado de baixo spin (LS - low-spin) é possível de ocorrer. A metaestabilidade obtida para estes sistemas DMS é estudada em conecção com as relaxações estruturais em torno da impureza, na ausência e na presença da vacância de oxigênio. Por fim, alternando respectivamente camadas magnéticas e não magnéticas de r-CrO2 e r-SnO2, foram estudados sistemas em uma configuração de super-rede (SL - superlattice), do tipo (CrO2)n(SnO2)n, com n = 1; 2; ...; 10 sendo o número de monocamadas. Para todos os valores de n foi observado comportamento meio-metal (half-metal) para os sistemas. O estado fundamental é ferromagnético (FM), com momento mangético igual a 2 mu_B por cromo ndependentemente do número de monocamadas. E como o óxido r-CrO2 é instável na temperatura ambiente, porém pode ser estabilizado, quando crescido sobre o r-SnO2, sugerimos que as super-redes (CrO2)n(SnO2)n podem ser aplicadas na tecnologia de spintrônica provendo eficiente polarização de spin de seus portadores. Os cálculos de estutura eletrônica foram realizados levando em conta a polarização de spin, usando o método PAW (Projector-Augmented-Wave) implementado no pacote computacional VASP (Vienna Ab-initio Simulation Package), dentro da teoria DFT (density functional theory) utilizando a aproximação local da densidade com correção GGA-PBE (generalized gradient corrections) e aproximação GGA-PBE+U, onde U é a correção on site de Hubbard. / Rutile tin dioxide (r-SnO2) is a wide-band-gap semiconductor and is part of a class of promising transparent conducting oxides (TCO). It has a direct band gap of 3.6 eV and shows n-type conductivity, even when not intentionally doped, which is usually attributed to intrinsic defects. On the other hand, it has been proposed theoretically that in spite of unintentionally doping, hydrogen impurities at interstitial or O substitutional sites could be responsible for the n-type conductivity in SnO2. In this work we report the results of ab initio electronic structure calculatations for pure tin dioxide as well as for some intrinsic defects VO, VSn, Sni, Oi, OSn, SnO, SnO+OSn and Sni+VO and for several H-related defect centers Hi, HO, HBC, Hi-Hi, Hi-HO, Hi-HBC, HBC-HBC, where V means vacancy and BC bond-centered sites. Our H-related results centers are confronted with those obtained for the intrinsic defects. Our findings show, for example, that hydrogen impurity has a donor character for all studied centers and that not only the isolated H, but also some of its complexes, show competitive low formation energies, suggesting that various H-related centers could be responsible for the n-type conductivity observed in the unintentionally doped SnO2. We discuss some results of the electronic and magnetic properties of TM-doped tin dioxide (TM = V, Cr, Fe, Mn, Co and Ni) in a diluted magnetic oxide configurations, i.e., Sn1-xTMxO2 and Sn1-xTMxO2-y(VO)y diluted alloys. As a prototype we will consider x = 0.04 and y = 0.02, which corresponds to a TM content just within the experimental window. Our aim is to analyze the presence of magnetic metastable states in these systems and how oxygen vacancies affect this metastability. For all cases, the ground state corresponds to the expected high spin (HS) configuration and a spin-crossover to the low-spin state is possible. The obtained magnetic metasbility in TM-doped SnO2 is discussed in connection with the structural relaxations around the impurity in absence and in presence of O vacancies. Finally, alternated magnetic and non-magnetic layers of rutile-CrO2 and rutile-SnO2 respectively, in a (CrO2)n(SnO2)n superlattice (SL) configuration, with n being the number of monolayers which are considered equal to 1, 2, ..., 10 are studied. A half-metallic behavior is observed for the (CrO2)n(SnO2)n SLs for all values of n. The ground state is found to be ferromagnetic (FM) with a magnetic moment of 2 µB per chromium atom, and this result does not depend on the number of monolayers n. As the FM rutile-CrO2 is unstable at ambient temperature, and known to be stabilized when on top of SnO2, we suggest that (CrO2)n(SnO2)n SLs may be applied to spintronic technologies since they provide efficient spin-polarized carriers. Spin-polarized electronic structure calculations were performed using the Projector-Augmented-Wave (PAW) method as implemented in the Vienna Ab-initio Simulation Package (VASP), within the spin density functional theory in the local density approximation with generalized gradient corrections (GGA-PBE) and GGA- PBE+U, where U is the Hubbard correction.
804

Frank Miller e os quadrinhos pelo que vale a pena morrer / Frank Miller and Comics: Worth dying for

Joaquim Cardia Ghirotti 04 July 2017 (has links)
As décadas de 1980-1990 marcam um ponto importante da história das revistas de quadrinhos de super-heróis. Mudanças no mercado, no público, na política, nos debates morais e culturais do momento ofereceram um cenário propício para novos desenvolvimentos no gênero super-herói. Esse quadro é cercado pelo pós-modernismo, a urbanização, a contracultura, mudanças nas artes e condições políticas que se desenvolvem da Guerra Fria, geograficamente centrada entre a Europa e os Estados Unidos, para a Guerra do Iraque, o que desloca atenções geopolíticas e conflitos para o Oriente Médio. Os quadrinhos de super-herói passam a oferecer maior liberdade temática para seus autores, e discussões sobre direitos autorais ganham força. Este cenário dispõe das condições para que alguns autores pudessem levar os super-heróis para discussões diferentes das décadas precedentes, permitindo o surgimento de trabalhos significativos, de autores que marcam suas obras abordando os super-heróis de forma pessoal. A presente pesquisa procura entender como o autor de histórias em quadrinhos Frank Miller se posicionou diante de uma determinada área de produção artística, utilizando-se das revistas de super-herói para discutir posicionamentos ideológicos, e reforçar seu caráter mítico e simbólico. Utilizando princípios da história cultural desenvolvidos por autores como Ginzburg, Burke, Gombrich, Schorske e Barzun, contextualiza-se a trajetória das histórias em quadrinhos até os anos 1980 e 1990, estabelecendo as condições da mídia quando do trabalho do artista. Para posicionar Miller em relação a seu mercado e suas relações de produção, são utilizadas as ferramentas analíticas de Michael Baxandall, que oferecem um modelo interpretativo das relações que se dão na produção artística. Finalmente, observam-se as abordagens temáticas e morais de sua obra, com seu contexto dentro de uma trajetória na história cultural. Para isso, traça-se um panorama que discute como a obra de Miller atualiza a jornada do herói de Joseph Campbell, utilizando-se da cultura popular para fazer um diálogo entre discussões morais, históricas e políticas, por meio de uma construção de narrativas heroicas que operam como mitos populares modernos e parâmetros civilizacionais, carregando em si princípios, valores e ideias de uma cultura. / The decades of 1980-1990 establish an important point in the history of comic books. Changes in the market, the audience, in politics and in the cultural and moral debates of the time offered a scenario which was welcoming to new developments on the super-hero genre. This moment is marked by post-modernism, urbanization, the counter-culture, changes in arts and the political conditions which develop from the Cold War, geographically centred between the United States and Europe, to the Iraq war, which moves the geopolitical attentions and conflicts to the Middle- East. Super-hero comics start offering wider thematic freedom to their authors, and discussions about creator\'s rights gain momentum. This scenario contains the conditions for some of these authors to take super-heroes to discussions which are different from the ones happening in the preceding decades, allowing the emergence of important works, made by authors which marked their work in a very personal manner. This research seeks to understand how comic-book author Frank Miller has positioned himself before an area of artistic production, using super-hero comics, and comics in general, to discuss moral positions, and to underline their mythical and symbolic character. Exercising principles of cultural history developed by authors like Ginzburg, Burke, Gombrich, Schorske and Barzun, we situate the trajectory of comics from their inception as magazines until the 1980s and 1990s, establishing which were the conditions of this medium when Miller produced the works we look at. To understand Miller in relation to his market and his production, we use the analytic tools of Michael Baxandall, which offer an interpretative model of the relationships which happen in artistic production. Finally, we observe the thematic and moral approaches of his work, with their context within a trajectory in cultural history. In order to perform this, we establish a context which discusses how Miller work updates Joseph Cambell\'s hero\'s journey, using popular culture to make connections between moral, political and historical debates, creating heroic narratives which operate as modern popular myths and as civilizational benchmarks, carrying with them the principles, values and ideas of a culture.
805

Analise de viabilidade de troca de motores eletricos superdimensionados e a influencia da energia reativa / Analysis of feasibility of the change of super dimensioned electrical engines and the influence of reactive energy

Castro, Renato Archanjo de 12 August 2018 (has links)
Orientador: Moacyr Trindade de Oliveira Andrade / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Mecanica / Made available in DSpace on 2018-08-12T05:28:32Z (GMT). No. of bitstreams: 1 Castro_RenatoArchanjode_M.pdf: 2548594 bytes, checksum: 5b0406363500b5419ff9f888bb2dac98 (MD5) Previous issue date: 2008 / Resumo: Este trabalho mostra a importância do motor elétrico de indução trifásico rotor gaiola de esquilo, que é o principal responsável pelo consumo de energia elétrica no seguimento industrial, apresenta suas características elétricas, mecânicas e sua evolução ao longo dos anos. Relata ainda a problemática do superdimensionamento destes motores instalados na indústria brasileira, ao qual tem como principal inconveniente a alta requisição de energia reativa nas instalações elétricas, apresenta a forma de faturamento da energia reativa aplicada pelas concessionárias de energia, bem como as principais formas de sua compensação aplicadas atualmente pela indústria nacional e seus efeitos na instalação existente. O trabalho apresenta uma análise comparativa entre o motor Alto Rendimento e o motor Standard, com ênfase na adequação da potência nominal em relação à demanda industrial, bem como em relação ao consumo de energia reativa das opções avaliadas. O suporte proveniente de pesquisas e adequações de instalações industriais aplicada pelo autor, em empresas da região de Americana, em relação à energia elétrica reativa, se constituem como base para o desenvolvimento deste trabalho. A conclusão final busca demonstrar que estas ações de conservação e adequação em motores elétricos industriais, são fundamentais para o processo de eficientização das próprias indústrias, do setor elétrico e da economia do país, de forma geral, uma vez que as mesmas implicam na redução da energia requerida pela indústria sem prover restrições a produção, resultando em significativa economia de recursos no provimento de novas fontes de energia e de melhor utilização do sistema elétrico nacional. / Abstract: This study shows the importance of the electrical engine of triphasic induction rotor (squirrel cage), which is the main responsible for the waste of electrical energy in industry; it presents its electrical and mechanical characteristics and its evolution along the years, as well. The study describes the problem of the super dimensioning of these engines that exist at the Brazilian industry, which has the major inconvenience of the high required of reactive energy in the electrical systems, presenting the way of invoicing the reactive energy applied by the power suppliers, as well as the main ways of its compensation applied presently by the national industry and its consequences for the existent installation. The study presents a comparative analysis between the engine of high efficiency and the Standard engine, focusing the adaptation of nominal power in relation to the industrial demand, as well as in what concerns the waste of reactive energy by the evaluated options. The background for the development of this study came from researches and adaptation of industrial plants applied by the Author in companies of the American region, as far as reactive electrical energy is concerned. The final conclusion tries to demonstrate that these actions of conservation and adaptation in industrial electrical engines are fundamental for the process of improving the efficiency of the very same industries, the electrical sector and the economy of the country as a whole, since that the same actions imply the reduction of the energy required by the industry without restrictions to the production, resulting into a significant saving of resources in what concerns the supply of new sources of energy and a better utilization of the national electrical system. / Mestrado / Mestre em Planejamento de Sistemas Energéticos
806

Explicit numerical schemes of SDEs driven by Lévy noise with super-linear coeffcients and their application to delay equations

Kumar, Chaman January 2015 (has links)
We investigate an explicit tamed Euler scheme of stochastic differential equation with random coefficients driven by Lévy noise, which has super-linear drift coefficient. The strong convergence property of the tamed Euler scheme is proved when drift coefficient satisfies one-sided local Lipschitz condition whereas diffusion and jump coefficients satisfy local Lipschitz conditions. A rate of convergence for the tamed Euler scheme is recovered when local Lipschitz conditions are replaced by global Lipschitz conditions and drift satisfies polynomial Lipschitz condition. These findings are consistent with those of the classical Euler scheme. New methodologies are developed to overcome challenges arising due to the jumps and the randomness of the coefficients. Moreover, as an application of these findings, a tamed Euler scheme is proposed for the stochastic delay differential equation driven by Lévy noise with drift coefficient that grows super-linearly in both delay and non-delay variables. The strong convergence property of the tamed Euler scheme for such SDDE driven by Lévy noise is studied and rate of convergence is shown to be consistent with that of the classical Euler scheme. Finally, an explicit tamed Milstein scheme with rate of convergence arbitrarily close to one is developed to approximate the stochastic differential equation driven by Lévy noise (without random coefficients) that has super-linearly growing drift coefficient.
807

Contribution à l’étude de l’amélioration de l’usinabilité des aciers – Analyse des Couches de Transferts Sélectifs (CTS) et caractérisation des conditions d’apparition / Contribution to the Study to the study of steel machinability enhancement bluilt-up layer analysis - characterization of occurrence conditions

Desaigues, Jean-Edouard 19 March 2015 (has links)
Les aciers contiennent de nombreuses inclusions : à base manganèse, aluminium, silicium, calcium, ... Leur impact individuel sur l'usinabilité demeure cependant mal connu dans la mesure où il est difficile de les isoler pour en quantifier l'influence. Une des premières tâches est de déterminer précisément leur effet tant sur le procédé en tant que tel (efforts, puissance, température) que sur les outils (nature et vitesse d'usure, modification chimique superficielle).De nombreuses études montrent la formation d'un « lubrifiant solide » à l'interface outil/copeau. Ce dépôt est parfois appelé « couche de transfert sélectif » (Build-up Layer). L'objectif de cette thèse est de savoir quels corps se cachent sous le vocable de « couche de transfert sélectif », quand cette couche est susceptible de se former, sur quelle partie de l'outil, dans quelles conditions opératoires, à quelle vitesse, pour quelles contraintes mécaniques et pour quelles températures. Est-elle susceptible de demeurer sur l'outil à l'arrêt de l'opération d'usinage ? A quelle vitesse se forme-telle ? Est-elle systématiquement stable ? / Steels contain many inclusions essentially composed by manganese, aluminium, silicium, calcium. However the influence of the different inclusions on machinability is poorly quantified. One of our first tasks is to determine precisely the effect of the inclusions on the metal cutting process as well as on the cutting tools. Many research works highlight a solid lubricant provided by the material being machined and spread along the tool-chip interface. This coating is so called Build up Layer.This thesis is devoted to answer some important questions: what is the actual composition of the Build-up Layer? Is it always the same for a tool machining a steel grade? When and where will the Build-up Layer appear? Is it stable?
808

Principes du repliement de la chromatine dévoilés par la microscopie super-résolue multi-couleurs / Principles of the Higher-Order Chromatin Folding Unveiled by Multicolor Superresolution Microscopy

Georgieva, Mariya 08 December 2015 (has links)
La relation entre le repliement du génome et les processus cellulaires majeurs, notamment la transcription, la réparation de l’ADN et la réplication est une question biologique centrale. De nouvelles méthodes de la génomique ont révélé un niveau inconnu de l’architecture tridimensionnelle de la chromatine. A l’échelle en dessous de la mégabase, certaines séquences génomiques se trouvent préférentiellement à proximité les unes des autres formant ainsi des domaines topologiques associés (TAD). Les gènes situés dans le même TAD ont des propriétés épigénétiques similaires, et leur expression au cours de la différentiation semble corrélée, ce qui suggère un lien fort entre la structure de la chromatine et la transcription. Les TADs sont à leur tour séparés par des régions avec peu de contacts, appelées frontières, qui sont généralement occupées par des protéines dites isolatrices. Les déterminants de cette organisation chromatinienne particulière et ses implications fonctionnelles sont largement méconnus. Selon une hypothèse récente, les TADs seraient formés par des contacts entre les séquences des frontières, stabilisés par la formation de boucles via les protéines isolatrices. Les travaux présentés ici ont pour but d’étudier le rôle des protéines isolatrices dans le mécanisme de formation des TADs chez la drosophile. La microscopie super-résolue a été implémentée et une série de développements ont été réalisés en microscopie à illumination structurée (SIM) et la microscopie à localisation de molécules uniques (SMLM), avec une attention particulière sur le marquage fluorescent. Ces développements ont directement été appliqués à l’étude de l’organisation nucléaire de la protéine associée aux éléments frontières (BEAF), une des 11 protéines isolatrices identifiées à ce jour chez la drosophile. Le fort enrichissement aux frontières des TADs, ainsi que son activité dans la formation de boucles d’ADN font de BEAF un candidat intéressant pour tester l’hypothèse de regroupement de frontières comme mécanisme général de repliement de la chromatine. La technique SMLM multi-couleurs a systématiquement localisé BEAF à la périphérie des larges domaines portant la marque épigénétique H3K27me3. L’analyse quantitative des images SMLM a révélé que BEAF forme des centaines de foyers d’une taille de 45 nm, composés en moyenne de 5 molécules, ce qui est en désaccord avec la présence de boucles de chromatine à large échelle. Afin de tester le regroupement de gènes directement au niveau de l’ADN, des frontières ont été marquées par des oligonucléotides fluorescents. Le nombre de foyers détectés par SIM s’est à nouveau révélé incompatible avec le modèle de contacts entre les frontières tout le long du génome. Par ailleurs, les distances entre paires de frontières au niveau de deux régions génomiques ont montré <5% de contacts. Ensemble, ces résultats sont en désaccord avec l’établissement d’interactions entre barrières chez la drosophile. Enfin, ces travaux de thèse ont contribué au développement méthodologique de la microscopie super-résolue, ce qui a permis d’apporter des preuves expérimentales invalidant le modèle de regroupement des frontières comme mécanisme général du repliement chromatinien. / The interplay between genome folding and key cellular functions such as transcription, DNA repair and replication is a fundamental question in chromatin biology. Recent genome-wide developments unveiled a new level of three-dimensional chromatin architecture. At the sub-megabase scale, some genome sequences are preferentially found in proximity with one another forming Topologically Associating Domains (TADs). Genes located within the same TAD display common epigenetic properties and tend to have coordinated dynamics of expression during differentiation, suggesting a strong link between chromatin structure and transcription. TADs are in turn separated by regions of low contact, termed TAD borders, which are generally occupied by factors called chromatin insulators. What are the determinants of this particular type of chromatin organization and what are the functional implications is still largely unknown. In an emerging hypothesis, TADs could be formed through contacts between TAD border sequences stabilized by the looping activity of insulator proteins. This thesis investigates the roles of insulator proteins in the TAD formation mechanism using Drosophila melanogaster as model system. Superresolution imaging was implemented and a series of developments were performed in Structured Illumination Microscopy (SIM) and Single-molecule Localization Microscopy (SMLM), with particular attention on fluorescent labeling for single-molecule detection. These developments were directly applied to study the nuclear organization of the Boundary Element Associated Factor (BEAF), one of the 11 insulator proteins discovered to date in Drosophila. The strong enrichment on TAD borders and its demonstrated looping activity make BEAF a potent candidate to test for the clustering of TAD borders as a general mechanism of chromatin folding. Multicolor SMLM systematically located BEAF foci at the periphery of large H3K27me3 chromatin domains. Quantitative analysis of SMLM images indicated BEAF forms hundreds of 45 nm foci, containing a mean of 5 molecules, which argues against a large-scale looping of BEAF-bound chromatin. To directly probe for gene clustering at the DNA level, TAD borders were labeled using fluorescent oligonucleotide probes. The number of foci detected by SIM was once more incompatible with a model of chromosome-wide contacting of multiple TAD borders. Furthermore, TAD border pairs distances were measured in two genomic regions, resulting in <5% of paired contacts among the measured barriers. Taken together, these results are inconsistent with constitutive interactions between consecutive or non-consecutive barriers in Drosophila.In conclusion, this study contributed to the methodological development of super-resolution microscopy which was applied to provide experimental evidence invalidating the TAD border clustering model as a general mechanism of chromatin folding.
809

Rendering Methods for 3D Fractals

Englund, Rickard January 2010 (has links)
3D fractals can be visualized as 3D objects with complex structure and has unlimited details. This thesis will be about methods to render 3D fractals effectively and efficiently, both to explore it in real-time and to create beautiful high resolution images with high details. The methods discussed is direct volume rendering with ray-casting and cut plane rendering to explore the fractal and an approach that uses super sampling to create high resolution images. Stereoscopic rendering is discussed and how it enhance the visual perception of the fractal
810

Développement de la réaction d’α-amidoalkylation catalytique directe des cétones catalysée par le super acide de Lewis Sn(NTf2)4 / Development of direct catalytic α-amidoalkylation of ketones catalyzed by super Lewis acid Sn(NTf2)4

Touati, Bahria 27 June 2014 (has links)
La chimie des ions N-acyliminiums est un axe de recherche important dans le domaine de la chimie moderne. L’apport de la catalyse, avec l’utilisation de super acides de Lewis, a permis d’améliorer de nombreux processus synthétiques. Nous avons pu développer des réactions d’α-amidoalkylation catalytiques directes de cétones et d’aldéhydes. Ces réactions sont catalysées par des charges très faibles (0.5-2 mol%) du super acide de Lewis, le triflimidate d’étain IV Sn(NTf2)4 et donnent accès à une large gamme de structures carbonylées très intéressantes, avec de bons rendements. L’influence des différents paramètres gouvernant la réactivité et l’étendue de la méthode ont été étudiées. Par la suite, le potentiel synthétique des produits obtenus a été étudié. Deux stratégies de réactions intramoléculaires ont été testées à partir d’adduits alkylés judicieusement fonctionnalisés, en utilisant soit des catalyseurs à base d’or, soit des catalyseurs de métathèse, permettant d’atteindre quelques structures polycycliques. / AThe chemistry of N-acyliminiums ions is one important area of research in modern organic chemistry. Lewis superacid catalysis has contributed in the improvement of many synthetic processes. We have developed some direct α-amidoalkylation reactions starting from unmodified ketones and aldehydes. The reactions were catalyzed by the Lewis superacid tin IV triflimidate, Sn(NTf2)4, at a very low catalyst loading(0.5-2 mol%), providing access to a set of very interesting carbonylated structures in good yields. The influence of different parameters governing the reactivity and the scope of the reaction were studied. Thereafter, the synthetic potential of the obtained products was evaluated. Two intramolecular approaches from suitably functionalized alkylated products were tested using gold and metathesis catalysts, allowed access to some polycyclic structures.

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