Following content removed due to copyright restrictions: Lambert, D. M., King, T., Shepherd, L. D., Livingston, A., Anderson, S. & Craig, J. L. (2005). Serial population bottlenecks and genetic variation: translocated populations of the New Zealand saddleback (Philesturnus carunculatus rufusater). Conservation Genetics 6: 1–14. Perrie, L. R., Shepherd, L. D.& Brownsey, P. J. (2005). Asplenium ·lucrosum nothosp. nov.: a sterile hybrid widely and erroneously cultivated as ‘‘Asplenium bulbiferum’’ Plant systematics and evolution 250: 243–257 / Ancient DNA was used to provide a temporal perspective for examining a number of evolutionary, conservation and cultural questions involving members of the New Zealand avifauna. Ancient mitochondrial DNA (mtDNA) sequences were used to examine the past levels and patterns of genetic diversity in the five species of New Zealand kiwi (Apterygidae). Brown kiwi, particularly in the South Island, exhibited high levels of genetic structuring with nearly every population exhibiting private mitochondrial haplotypes. The extinction of a large number of brown kiwi populations has, therefore, led to the loss of a large amount of genetic variation in these species. The past ranges of great spotted kiwi and the three brown kiwi species, whose bones are morphologically indistinguishable, were determined. This information can aid conservation programmes aiming to re-introduce kiwi to regions where they are now extinct. In contrast to the high level of genetic structuring in South Island brown kiwi, the majority of little spotted kiwi samples from the South Island shared a common haplotype. The difference in phylogeography between brown kiwi and little spotted kiwi is hypothesised to relate to differences in their dispersal behaviour and/or their population histories. The addition of ancient samples of little spotted kiwi from the North Island indicated a complex relationship with great spotted kiwi. Nuclear microsatellite DNA markers were isolated from North Island brown kiwi and tested for cross amplification in the other kiwi species. Five loci were polymorphic in all kiwi species. Preliminary analyses of genotyping results indicated that the kiwi species were distinguished by assignment tests and that subdivision may occur within several of the species. An extensive reference database of modern and ancient mtDNA sequences was used to determine species and provenance of a number of unlabelled museum subfossil bones and skins. This method was also used to examine provenance of brown kiwi feathers from Maori artefacts (cloaks and baskets). Ancient DNA methodology was also used in a molecular examination of the relationships of a second endemic avian family, the New Zealand wattlebirds (Callaeatidae). Analyses of nuclear gene sequences, c-mos and RAG-1, revealed kokako, saddleback and huia comprised a strongly supported monophyletic group. A divergence time estimate for the New Zealand wattlebirds indicated that they are more likely to have arrived by transoceanic dispersal than have a Gondwanan origin. Sequences from three mtDNA genes, 12S, ND2 and cytochrome b, were also analysed but could not resolve the relationships between the three genera. Microsatellite DNA from the extinct New Zealand huia exhibited considerable genetic variation, exceeding that found in extant North Island saddleback, from which the loci were isolated. Assignment tests indicated no genetic structuring within huia, although interpretation was complicated by a lack of provenance details for many of the skins. The results presented here suggest that ancient DNA can not only provide information about the relationships of extinct taxa but also demonstrates the importance of placing the present day genetic diversity found in endangered taxa within the context of past patterns and levels of genetic variation.

Thesis (Master of Arts)--Miami University, Dept. of Comparative Religion, 2009. / Title from first page of PDF document. Includes bibliographical references (p. 63-66).

No description available.

The subject of the diploma thesis is the design and process of the construction-technical part of the project documentation for realization of the new building of the mountain guest-house house in Karolina with an accommodation capacity of 36 people. The building is located on a slope, it is a basement, it has 2 underground floors and 3 aboveground floors. The building is roofed with a saddle roof with a concrete roofing. Vertical support structures are made from the Porotherm system. In addition to the project, an architectural study, a fire report and a thermal-technical assessment of the house are also included.

60% of primates in the world are threatened with extinction, while 75% have declining populations. The biggest threats to primates are the result of human activity. In this study, I have been looking at the primate Spix’s Saddleback tamarin (Leontocebus fuscicollis) and tried to estimate the home range size of four different study groups, while also trying to assess if there are differences in the type of trees in the areas where the tamarins are present compared to where they are absent. I have also compared the behaviour of the four different study groups. The data was collected in the Tambopata nature reserve in the Madre de Dios region in Peru during the summer of 2022, with the help and support from the research organisation Fauna Forever. To complement the observations, previously collected data from 2019-2021 was also used. The tamarins were followed using a group follow method, and the behaviour was noted every 10 minutes using an instantaneous scan sampling method. The tree composition data was gathered from previously created tree plots, made by the Fauna Forever staff. I found that the home range sizes of the four study groups varied between 2.6 ha and 17.9 ha. The data from the tree plots showed no statistically significant difference in where the tamarins chose to live. There was also no statistically significant difference between the four study groups in behaviour, except for how high up in the trees they groups spent their time. There was a lack of good data in this study, due to time- and material constraints, but overall the home ranges were of close to similar size as earlier studies, and the behaviour of the tamarins was in general also consistent with earlier studies. More research would be needed to answer the further questions that arose during this study concerning the ecological constraints of the tamarins home range.

No description available.

The choice of a place to live and reproduce is crucial for species� survival in providing them with adequate resources and shelter from predators or climatic conditions. Determining habitat suitability in endangered species is important for the success of translocation as a conservation tool. In addition, understanding mechanisms (source/sink system versus ecological traps) that drive habitat selection in translocated animals may be critical to population viability. In New Zealand, where ecosystems are highly vulnerable to extinction, habitat restoration on predator-free off-shore islands is an important recovery tool. Therefore, there is a need to understand the relationship between the establishment of the translocated populations and the characteristics of their new environment. Previous research indicated that re-introduced populations of Stewart Island robin (Petroica australis rakiura - Toutouwai) and South Island saddleback (Philesternus carunculatus carunculatus - Tieke) on Ulva Island (Stewart Island), New Zealand, showed preferences for coastal habitats that were characterized by low-lying dense vegetation and open ground cover. In this study, we further investigated territorial establishment in these two populations since re-introduction and looked at how birds utilised the landscape. I hypothesised that sites colonised soon after re-introduction were of high quality and later on, birds moved into unsuitable habitats. I defined habitat quality at a micro-scale in terms of vegetation structure, nest characteristics and food availability. I modeled bird presence and nesting success in relation to habitat components to determine factors in the environment that influenced breeding site selection and contributed to successful nesting in these two species. I discussed results in comparison to similar bird-habitat models developed for the South Island saddleback population on Motuara Island (Marlborough Sounds) and examined explanatory variables in each model. Translocated birds in the three studied populations first established territories in coastal scrub, and in the following years moved into larger coastal forest stands. Although vegetation structure was the primary variable explaining site selection in these populations, vegetation composition should still be considered important as it dictated the suitability of nesting substrate and the availability of food items. There was no evidence that first-colonised areas were more suitable habitats, and I concluded that these cases could not be used as examples of ecological traps. Instead, results suggested that with increased density robins and saddlebacks on Ulva have more recently settled in sites less suitable to nesting and foraging, thus underlying a source/sink structure. However, the sparse distribution of food items on Motuara contributed to a lack of territorial behavior and environmental effect on breeding success; therefore a source/sink system could not be confirmed in this population. I recommended that future translocation sites give preference to mixed-size stands with broadleaved species that are characterised by dense canopy below 4 m height and with suitable cavities in live trees. Lastly, due to robins� and saddlebacks� attraction to conspecifics and their territorial behavior, resources evenly distributed across the landscape could also increase their survival and reproductive success.

The choice of a place to live and reproduce is crucial for species� survival in providing them with adequate resources and shelter from predators or climatic conditions. Determining habitat suitability in endangered species is important for the success of translocation as a conservation tool. In addition, understanding mechanisms (source/sink system versus ecological traps) that drive habitat selection in translocated animals may be critical to population viability. In New Zealand, where ecosystems are highly vulnerable to extinction, habitat restoration on predator-free off-shore islands is an important recovery tool. Therefore, there is a need to understand the relationship between the establishment of the translocated populations and the characteristics of their new environment. Previous research indicated that re-introduced populations of Stewart Island robin (Petroica australis rakiura - Toutouwai) and South Island saddleback (Philesternus carunculatus carunculatus - Tieke) on Ulva Island (Stewart Island), New Zealand, showed preferences for coastal habitats that were characterized by low-lying dense vegetation and open ground cover. In this study, we further investigated territorial establishment in these two populations since re-introduction and looked at how birds utilised the landscape. I hypothesised that sites colonised soon after re-introduction were of high quality and later on, birds moved into unsuitable habitats. I defined habitat quality at a micro-scale in terms of vegetation structure, nest characteristics and food availability. I modeled bird presence and nesting success in relation to habitat components to determine factors in the environment that influenced breeding site selection and contributed to successful nesting in these two species. I discussed results in comparison to similar bird-habitat models developed for the South Island saddleback population on Motuara Island (Marlborough Sounds) and examined explanatory variables in each model. Translocated birds in the three studied populations first established territories in coastal scrub, and in the following years moved into larger coastal forest stands. Although vegetation structure was the primary variable explaining site selection in these populations, vegetation composition should still be considered important as it dictated the suitability of nesting substrate and the availability of food items. There was no evidence that first-colonised areas were more suitable habitats, and I concluded that these cases could not be used as examples of ecological traps. Instead, results suggested that with increased density robins and saddlebacks on Ulva have more recently settled in sites less suitable to nesting and foraging, thus underlying a source/sink structure. However, the sparse distribution of food items on Motuara contributed to a lack of territorial behavior and environmental effect on breeding success; therefore a source/sink system could not be confirmed in this population. I recommended that future translocation sites give preference to mixed-size stands with broadleaved species that are characterised by dense canopy below 4 m height and with suitable cavities in live trees. Lastly, due to robins� and saddlebacks� attraction to conspecifics and their territorial behavior, resources evenly distributed across the landscape could also increase their survival and reproductive success.

Many countries in the global south - which harbour the majority of the world’s biodiversity - face serious resource limitations and a lack of access to affordable sequencing services. Furthermore, biodiversity research and monitoring of non-model, threatened and/or cryptic species often relies on low-quality non-invasive genetic samples. In situ conservation genomics approaches optimised for field conditions and low-quality DNA can help empower local researchers and meet their needs. To do so, however, accessible and reproducible sequencing and genotyping alternatives are needed. I designed a SNP panel as a field-friendly genotyping approach for two species of Amazonian primates using both high- and low-quality DNA samples, and two different sequencing platforms, Illumina and Nanopore. I used 14 high-quality genomes to identify a set of 210 SNPs that allow for identification of species (twelve SNPs), sex (twelve SNPs) and individual identity (186 SNPs) in two species of tamarins, Leontocebus weddelli and Saguinus imperator. Primers, adapters and indexes were designed in a Genotyping-in-Thousands by sequencing approach that is compatible with both sequencing platforms. This approach is based on sequencing multiplexed PCR products of a few hundred target SNPs to genotype thousands of individuals in a single sequencing run. In an effort to make conservation genomics more accessible, the reproducible pipeline to obtain the informative SNPs is being modulated with Snakemake, a workflow management system. / Muchos países en el sur global - los cuales poseen la mayoría de la biodiversidad mundial - enfrentan serias limitaciones de recursos y una falta de acceso a servicios económicos de secuenciación. Con frecuencia, la investigación y el monitoreo de biodiversidad y especies no-modelo, amenazadas y/o crípticas, dependen de muestras genéticas no-invasivas de baja calidad. La genómica de la conservación in situ optimizada para condiciones de campo y ADN de baja calidad puede empoderar a investigadorxs locales y ayudarles a responder a sus necesidades. Para ello, sin embargo, se requieren alternativas accesibles y reproducibles de secuenciación y genotipado. Diseñé un panel de SNPs como una aproximación de genotipado apta para el campo y dirigida a dos especies de primates amazónicos con el uso de ADN de baja y alta calidad, y dos plataformas de secuenciación (Illumina y Nanopore). Usé 14 genomas de alta calidad para encontrar 210 SNPs que permiten la identificación de la especie (doce SNPs), del sexo (doce SNPs) y de la identidad individual (186 SNPs) en dos especies de pichicos, Leontocebus weddelli y Saguinus imperator. Los cebadores, adaptadores e índices fueron diseñados con un enfoque de Genotyping-in-Thousands by sequencing (Genotipado en los miles por secuenciación) que es compatible con ambas plataformas de secuenciación. Este método está basado en la secuenciación de productos de PCR multiplexados de unos cientos de SNPs para genotipar miles de individuos en una sola corrida de secuenciación. En un intento de mejorar la accesibilidad de la genómica de la conservación, el proceso reproducible para obtener a los SNPs informativos está siendo modulado con Snakemake, un sistema de manejo de flujos de trabajo.

Text in German / Die vorliegende Forschungsarbeit beschäftigt sich mit der erlebnisorientierten Ausrichtung des geistlichen Lebensstils als Herausforderung für den Gemeindeaufbau in der Postmoderne. Die zunehmende Erlebnisorientierung der postmodernen Gesellschaft hat auch vor den Türen der Glaubensgemeinschaften in Deutschland und in der Schweiz nicht Halt gemacht. Die Folgen fordern von den Verantwortlichen der Gemeinden und Kirchen neue Überlegungen im Hinblick auf die bisherige Gemeindeaufbaupraxis. In diesem Prozess fällt auf, dass in Deutschland und in der Schweiz vor allem die Impulse der Church Growth Movement (CGM), insbesondere das Gemeindemodell der Willow Creek Community Church (WCCC) und der Saddleback Valley Community Church (SVCC), große Aufmerksamkeit erlangt haben. Einerseits werden die Gemeindemodelle vielerorts mit Begeisterung nachgeahmt, andererseits werden sie aber unter anderem wegen ihres Verhältnisses zur Erlebnisorientierung und wegen ihres Umgangs damit stark kritisiert. In der vorliegenden Forschungsarbeit wurden deshalb die Gemeinsamkeiten und Unterschiede bezüglich der erlebnisorientierten Ausrichtung des geistlichen Lebensstils der Gläubigen in den Partnergemeinden der Willow Creek Community Church und in den Nichtpartnergemeinden empirisch untersucht. Außerdem wurde geprüft, inwiefern soziostrukturelle Faktoren, insbesondere das Gemeindeumfeld, zur Generierung des erlebnisorientierten geistlichen Lebensstil beitragen und, inwiefern eine Wechselwirkung zwischen den Bestandteilen des geistlichen Lebensstils vorhanden ist. Die Ergebnisse der Forschungsarbeit ermöglichen eine empirisch-oikodomische Beurteilung der Gemeindeaufbaumodelle der CGM und die Entwicklung einer Vorgehensweise im Gemeindeaufbau in der Postmoderne, wodurch auf die zunehmende Erlebnisorientierung der Gesellschaft als Herausforderung in der Gemeindeaufbaupraxis entsprechend reagiert werden kann. The present research investigates the justification of an experience-orientated alignment of a spiritual lifestyle as a challenge for churchbuilding in postmodernity. The increasing experience-orientated behaviour in post-modern society did not stop at the gates of the churches in Germany and Switzerland. The results require, from those persons responsible in the congregations and churches, new deliberation and reflection with regard to the present practice of the promotion and development of church growth. When one looks at this process, it becomes apparent, that above all the stimuli from the Church Growth Movement (CGM), especially the church models of the Willow Creek Community Church (WCCC) and the Saddleback Valley Community Church (SVCC) gained huge attention in Germany and Switzerland. On the one hand the church models are emulated with great enthusiasm, on the other hand they are strongly criticised for their relationship with and handling of experience-orientated behaviour. The present research empirically examines and investigates the communalities and differences regarding the experience-orientated alignment of the spiritual lifestyle of the faithful believers from partner churches of the Willow Creek Community Church and non-partner churches. It was also examined and investigated to what degree socio-structural factors contributed to, particularly the congregational environment, with regards to the creation of the experience-orientated spiritual life style, and in how far an interaction exists between the various components of the spiritual lifestyle. The research findings facilitate an empirical-theological evaluation and assessment of the models of churchbuilding of the CGM and the development of an approach to churchbuilding in postmodernity. It is therefore possible to react to the increasing experience-orientation of the societal as a challenge in the churchbuilding. / Practical and Systematic Theology / D.Th. (Practical Theology)