Diss. (sammanfattning) Umeå : Sveriges lantbruksuniv. / Härtill 5 uppsatser.

Diss. (sammanfattning) Umeå : Sveriges lantbruksuniv. / Härtill 4 uppsatser.

Thesis (M.A.)--Boston University / The morphology of the cellular constituents of the blood of Salmo trutta was investigated. Blood cell counts and differential counts were included. Cellular elements of the blood were found to be nucleated erythrocytes, lymphocytes, neutrophils, monocytes and thrombocytes. Dried smears of blood which had been stained with Wright's stain indicated the erythrocytes were flat elliptical cells . However, the phase microscope revealed the biconcave shape of the mature erythrocyte, the concavity being interrupted by the central nucleus. Average cell rreasurements were 16.5 microns in length and 10.2 microns in width. [TRUNCATED]

For Atlantic salmon (Salmo salar) and brown trout (Salmo trutta), the decision to migrate or when to migrate is believed to be influenced by the individual’s metabolic rate (MR) relative its food intake. As MR was expected to be related to behaviour, the potential links between behaviour and metabolic costs was studied. For both salmon and trout the dominant individual had a higher standard metabolic rate (SMR) than its subordinate counterpart. Also, successful migrants of brown trout had a higher SMR than unsuccessful migrants, whereas no such difference was found for obligate migratory Atlantic salmon. Measures of variation in MR and boldness indicated that Atlantic salmon was more sensitive to stress than brown trout and became passive when stressed. When two trout were interacting, an increase in ventilation rate (VR) was positively correlated to fighting intensity. The first day after an interaction, VR did not differ between small dominant and subordinate trout (mean size 3.7g), whereas for large trout (26.0g) subordinates had higher VR than dominants. However, a combination of low temperature (10°C) and high water velocity (22cm/s) eliminated this difference. This probably reflects the high swimming activity of small dominants and the low motivation for dominants to defend a large territory when temperatures were low and the cost of moving was high. These results show that the relationship between MR and behaviour may differ depending on species, fish size and environmental factors.

The brown trout (Salmo trutta) is an ecologically, economically, aesthetically fish species whose poor conservation status in the European countries calls for further attention and action. The continuing erosion of the genetic resources of brown trout populations by human activities calls for strategies to reverse the current trend. We studied a genetic diversity of population of brown trout in the territory of Czech R. and in Slovakia using genetic markers. In the fragments of mitochondrial DNA (gen for ND-5/6) and nuclear DNA (gen for LDH1) amplified through PCR the differences were searched with the use of RFLP. In tested populations seven haplotypes were founded, four haplotypes were represented in almost of all populations. The ``Danubian haplotypes{\crqq} were strictly confined to the Danubian and Vistula drainages, the ``Atlantic haplotypes{\crqq} dominated in all populations, most of the total molecular variance (72 %) was attributed to differences within populations. Two alleles at LDHC1٭ - the ancestral ٭100 and ٭90 (at a high frequency) were revealed. This genotypic replacement is considered to be due to anthropogenic activities.

Little is known about the behavior of juvenile brown trout (Salmo trutta) during winter in boreal streams. Harsh winter conditions presumably affect overwinter survival negatively in small streams, where ice may have a large effect on survival of young salmonids. The aim of this study was to examine the effect of over-head ice cover on the spatial positioning of small brown trout in a boreal stream. I hypothesized that during the day brown trout will use the middle of the stream to a greater extent in the presence of over-head ice cover than in its absence. In the absence of ice cover, trout were predicted to hold station near the shore. At night, I hypothesized that there will be little effect of ice cover on the spatial positioning of the trout, with trout expected to occupy the entire stream width. Analysis of the results for the entire study period revealed a strong positive correlation between the tendency to be positioned towards the middle of the stream during daytime and the presence of ice cover. An analysis based on the period when water temperature was around 0o C (< 0,3o C) showed that trout used the entire width of the stream in the presence of surface ice but were restricted to the margins in the absence of ice. My hypothesis regarding the spatial distribution of trout at night was not supported by the data, as there was no difference in their spatial distribution during the day and at night. / Det råder stor okunskap om hur vinterförhållanden påverkar överlevnaden av juvenila öringar (Salmo trutta) i boreala områden. Isformationer och dess bildningsprocesser kan ha avgörande effekt på mortaliteten hos unga öringar som övervintrar i mindre vattendrag. Denna studie handlar om istäckets påverkan på öringens positionering längs vattendragets bredd. Hypoteserna är 1) att närvaron av istäcket ger öringen större möjlighet att kunna utnyttja mer metaboliskt gynnsamma ståndplatser i vattendraget än utan is och 2) att istäcket har effekt på daglig respektive nattlig positionering ifrån strandkanten. Resultaten i studien visar ett starkt positivt förhållande mellan ökad andel istäckningsgrad och positionering längre ut från strandkanten mot mitten av vattendraget. Vidare visar mina resultat, som baseras på perioden när vattentemperaturen var runt 0o C (< 0,3o C), att öringen använde sig av hela vattendragets bredd i närvaro av istäcke, men var nära strandkanten vid frånvaro av istäcket. Min hypotes angående öringens rumsliga fördelning under natten stöds inte av resultatet, då det inte fanns någon skillnad i deras rumsliga fördelning under dagen respektive på natten.

La truite commune Salmo trutta L. est l'espèce de salmonidés la plus rependue en Europe. Cette espèce présente une grande diversité phénotypique liée à son histoire évolutive complexe. Chaque année, d’intenses repeuplements ont lieu afin d’augmenter les densités locales de populations, notamment pour la pêche sportive. Des truites d’origine atlantique, domestiquées depuis des décennies, et plus récemment des souches domestiques méditerranéennes, sont largement utilisées pour repeupler les populations sauvages locales d’origine méditerranéenne dans le sud de la France. Jusqu’à présent, les conséquences des interactions génétiques, telles que l’hybridation et l’introgression d’allèles domestiques dans les populations locales résultants de ces repeuplements, étaient suivies à l’aide de marqueurs allozymes et microsatellites. Cependant, en raison de leurs nombres extrêmement réduits, ces marqueurs n’offraient qu’une représentation très partielle du génome. Ainsi, leur étude ne permet pas de rendre compte fidèlement des signatures génomiques associées aux pratiques de repeuplement, nécessaires pour comprendre les conséquences évolutives de l’introgression. L’objectif de cette thèse est donc d’étudier à l’échelle génomique les conséquences des interactions génétiques induites par l’introduction d’individus domestiques d’origines atlantique et méditerranéenne dans les populations ‘sauvages’ méditerranéennes du bassin de l’Orb. La première partie de cette thèse rend compte du développement d’environ 196000 marqueurs SNPs et d’une carte de liaison génétique haute densité chez S. trutta. Dans la deuxième partie, les outils moléculaires précédemment développés sont utilisés pour détecter à l’échelle individuelle les haplotypes introgressés et ainsi décrire le paysage génomique de l’introgression dans trois populations sauvages du bassin de l’Orb. La distribution de la taille de ces haplotypes est alors utilisée en prenant en compte les variations du taux local de recombinaison pour estimer l’âge moyen de l’introgression dans chaque population locale. Finalement, la troisième partie s’intéresse aux pressions sélectives - positives ou négatives - qui modulent le paysage génomique de l’introgression d’allèles domestiques dans les populations sauvages. Les résultats suggèrent que les conséquences de l’hybridation sur la valeur sélective des individus doivent être considérées séparément entre le court et le long terme. Ces travaux montrent que la compréhension des mécanismes évolutifs impliqués présente un intérêt majeur pour la conservation et la gestion des populations naturelles. / The brown trout Salmo trutta L. is the most widely distributed salmonid species in Europe. The species presents a high level of phenotypic diversity linked to its complex evolutionary history. An Atlantic hatchery lineage, which has been domesticated for decades, and more recently a domesticated Mediterranean strain, have been largely used for restocking and enhancement of wild Mediterranean populations in southern France, especially for recreational fishing. The impact of restocking practices on brown trout genetic diversity and population genetic structure has been extensively studied with allozyme and microsatellite markers. However, the small number of genetic markers used in these studies did not allow to get a genome-wide representation of introgression. The aim of this thesis was to assess the genome-wide impact of repeated introductions of Atlantic and Mediterranean domesticated strains into wild Mediterranean populations. First, a large number of SNP markers have been developed as well as a high density genetic map for S. trutta. Secondly, the molecular tools previously developed have been used to detect introgressed haplotypes and to provide a detailed picture of introgression frequency patterns across the genome of three wild populations from the Orb drainage. The length distribution of admixture tracts was used to determine the timing of introgression, taking variation in local recombination rate into account. Finally, this study focused on the positive or negative selective forces that modulate the genome-wide landscape of introgression. Our results suggest that the consequences of hybridization on individual fitness have to be considered separately over short and long timescales. This work shows that understanding the evolutionary consequences of stocking practices is of major interest for the conservation and management of natural populations.

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Winter has been generally considered as a bottleneck period for salmonid populations, but recent studies show it might be more context related. The purpose of this study was to examine how surface ice changes spatial distribution of juvenile one-year-old brown trout in a small boreal stream. I hypothesized that the presence of surface ice will allow a more even distribution of trout over the entire width of the stream while in the absence of ice, trout will be more heavily associated with near-edge habitats. I also hypothesized trout will be more evenly dispersed at night over the width of the stream even in the absence of surface ice. My results show a strong positive correlation between icecover and spatial distribution. In the presence of surface ice trout use the whole width of the stream, while in the absence of ice the middle regions of the stream were almost completely devoid of fish. My results also show there was no difference in the spatial distribution between night and day in the presence of ice cover, but in the absence of ice cover trout were more tightly associated with the stream edge during day whereas at night they were more evenly dispersed over the entire width of thestream. / Vinter har i allmänhet ansetts vara en flaskhals-period för laxfiskar, men nya studier visar att det kan vara mer sammanhangsrelaterat. Syftet med denna studie var att undersöka hur istäcket påverkar distribution av unga ett-åriga öring längs vattendragets bredd i en liten boreal ström. Jag hypotiserade att närvaron av istäcket kommer att möjliggöra en jämnare fördelning av öring över strömmens bredd. Min andra hypotes var att öring kommer att sprida sig mer jämnt på natten över strömmens bredd även vid frånvaro av istäcke. Mina resultat visar en stark positiv korrelation mellan istäckningsgrad och rumslig distribution. Vid närvaro av istäcke använde öring mer effektivt habitater över hela bredden av vattendraget, medans i frånvaro var de habitater närmare mitten av vattendraget nästan helt tomma av fisk. Mina resultat visar också att det inte fanns någon skillnad i rumslig fördelning mellan natt och dag i närvaro av istäcke men i frånvaro av istäcke var öring mer tätt associerad med strömkant habitater under dagen och på natten var de mer jämnt spridda över bredden av strömmen.