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Characterisation of protein-phospholipid interactions in implantable delivery systemsTantipolphan, Ruedeeporn, n/a January 2007 (has links)
Purpose: This thesis aimed to gain a better understanding of the effects of salts in modifying in vitro phase behaviour of lecithin and cholesterol solid implants and to obtain further information on in vitro protein release and stability.
Methods: Raman spectroscopy and partial least squares regression (PLSR) were used to investigate lecithin-cholesterol molecular interactions as a function of method of preparation. Lipid-salt interactions were studied by attenuated total reflectance Fourier transform infrared (ATR-FTIR) and Raman spectroscopy using principal component analysis (PCA). In vitro release of bovine serum albumin (BSA), a model protein, from lecithin and lecithin:cholesterol implants comprising 10 and 30% NaCl and CaCl₂ were performed. Size exclusion (SE) HPLC was used for quantitative and qualitative analysis of the released BSA. On hydration, changes in phase behaviour and implant morphology were studied by ATR spectroscopy and light microscopy. SE-HPLC, ATR and fluorescence spectroscopy were used to evaluate the structure of unreleased BSA. Protein adsorption on lipid films was studied by flow through ATR spectroscopy. Increased amide II peak area upon recirculation of BSA in salt solutions over hydrated lecithin and lecithin:cholesterol films cast on ZnSe prisms was used to quantify the deposition of BSA onto the lipid surfaces.
Results: Shifts in the Raman spectra suggested the lecithin headgroup may be involved in lecithin-cholesterol interactions. Greater R� and root mean square error of cross validation in the calibration curves of physical mixing and heating (120�C) methods reflected poor mixing in these preparations. The mean absolute residue and mean Mahalanobis distance values from the physical mixing and granulation methods indicated their spectral similarity and comparable level of lecithin-cholesterol interactions. Calcium exhibited stronger affinity for phospholipids than sodium and it induced headgroup hydration and reorganisation upon binding. PCA of ATR spectra was sensitive to cholesterol addition, calcium binding and method of preparation whilst PCA of Raman spectra only differentiated the presence of cholesterol. In vitro release of BSA from implants produced from wet granulation mixtures of lecithin and lecithin:cholesterol in the absence of salt showed retention of a high monomer content and the release profiles were similar to the literature. Cholesterol increased the swelling, induced phase transformation of lecithin and, subsequently, reduced the BSA release. Salts only slightly modified the BSA release from the lecithin implants. In contrast, for lecithin:cholesterol matrices salts greatly enhanced implant swelling, induced the formation of hydrated lecithin of heterogeneous size and inhibited the in vitro BSA release. Analyses of the protein showed increased aggregation of BSA with a high retention of native structure while retained within the swollen matrices. ATR spectra suggested that salts promoted protein adsorption onto hydrated lecithin surfaces and the effects depend on salt types (NaCl > CaCl₂) and concentration (0.1 M > 1.0 M) but not on lecithin:cholesterol surfaces.
Conclusion: PLSR and PCA can be used to investigate molecular interactions in the solid lipid matrices. In lecithin:cholesterol implants, salts modified the phase behaviour of lecithin which resulted in enhanced swelling, formation of hydrated lecithin of altered morphology and inhibition of in vitro BSA release.
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Interaction between the effects of sodium chloride and high temperature on the vegetative growth of tomato (Lycopersicon esculentum Mill.) / by Ding Xiang Wang.Wang, Ding Xiang January 1993 (has links)
Bibliography: leaves 151-187. / xvii, 187 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Agriculture and Natural Resource Sciences, 1993
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Temperate urban mangrove forests : their ecological linkages with adjacent habitatsYerman, Michelle N., University of Western Sydney, College of Science, Technology and Environment, School of Natural Sciences January 2003 (has links)
Estuarine habitats along the temperate south-eastern shores of Australia are generally made up of salt marsh, mangrove forests and seagrass beds. In urban areas these habitats have been progressively fragmented as a result of population increase and industrial expansion. Salt marshes in particular have been vulnerable to urban expansion and reclamation because of their close proximity to densely populated areas, while mangrove forests have been less often reclaimed because of frequent tidal inundation. The effect of reclamation of salt marshes on the biotic assemblages and functioning of mangrove forests with an adjacent salt marsh, park or bund wall was examined at nine separate locations on the Parramatta River, Sydney NSW. A mensurative approach was used to describe the patterns of distribution and abundance of macro fauna at several temporal and spatial scales. The implications for management are that salt marshes are an integral part of estuaries, and smaller patches of salt marsh are just as important as larger patches in maintaining the diversity of faunal assemblages and ecosystem functioning in mangrove forests in urban areas / Master of Science (Hons)
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Interaction between the effects of sodium chloride and high temperature on the vegetative growth of tomato (Lycopersicon esculentum Mill.)Wang, Ding Xiang. January 1993 (has links) (PDF)
Bibliography: leaves 151-187.
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The Effect of Mechanical Characteristics of Basal Decollement and Basement Structures on Deformation of the Zagros BasinBahroudi, Abbas January 2003 (has links)
<p>Two fundamental structural elements, basement architecture and basal decollement, play a significant role in the evolution of a tectonically active region. Using different approaches (field data, literature review and analogue models) this thesis demonstrates that these two elements affected the deformation style in the Zagros fold-thrust belt during Mesozoic extensional and Cenozoic contractional episodes. </p><p>Reassessment of available data suggests a new configuration for the basement to Zagros basin that consists of basement faults with three different trends. Complicated interrelationships of these basement faults divide the Zagros basin into two major basement blocks, active and passive. This model associated with geological evidence suggests that deformation in the basement due to the convergence between Arabia and Iran is not restricted to the Zagros Mountains but already involves a considerable part of the Arabian platform. However, deformation in the cover units is not only governed by the deformation in the basement, which are decoupled from each other by the Hormuz salt in many parts in the Zagros fold-thrust belt. </p><p>Geological evidence shows that there is a clear relationship between activity of the Hormuz structures and the basement faults in the Zagros basin. Extended analogue models indirectly show how Hormuz types of basal decollement associated with the opening of Neo-Tethys can control the distribution, number, width and geometry of faults, penetrative strain, and diapirism. Analogue models shortened from one-end show that the spatial distribution of the Hormuz salt in the Zagros belt map viscous and frictional decollements to the thin-skinned deformation. Shortening of the cover results in formation of partitioning of strain into transpressional zones, different topographic wedges and differential sedimentation of growth sediments along the Zagros belt. Model results supported by geological and geophysical data sets suggest that some of the faults previously attributed to basement could have developed above the initial lateral boundaries between viscous and frictional basal decollements.</p>
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Factors influencing the atmospheric aerosol composition at two sites in western OregonKo, Lih-jong 26 June 1992 (has links)
Fine and coarse particles were collected for eight weeks during the
summer of 1991, at a coastal site (Yaquina Head) and a non-industrial site
(Corvallis) in Western Oregon to characterize the aerosol composition and
evaluate whether the sites are appropriate for sampling "background" marine
air. Concentrations of up to 11 species (S0₄²⁻, NO₃⁻, Cl⁻, Na, Fe, Ni, Pb, Cr, Co,
Sb, and CH₃SO₃H) for 95 samples were determined using four chemical
analysis techniques.
The influences of seasalt and soil dust were identified by analyzing
concentrations of Na and Fe in the aerosol samples. Relative elemental
composition in fine and coarse fractions indicated that the aerosol
composition at Yaquina Head was greatly affected by seasalt. "Seasalt"
enrichment factors (relative to Na) indicated that seasalt is the only source of
Cl⁻ and SO₄²⁻ in coarse particles at Yaquina Head. In contrast, the seasalt
influence was relatively weak at the Corvallis site. "Crustal" enrichment
factors suggested that soil dust was not a major source of Na⁺, Cl⁻, or S0₄²⁻ at
either site.
A simple conceptual model that relies on meteorological conditions was
used to identify sampling periods with long range transport from either
marine or continental areas as well as local influences. This model suggested
that during 61% of the experiment period the aerosols were advected from
marine areas. At Yaquina Head, 52% of the sampling periods are associated
with the "clean" background air (marine air with no local influences). Thus,
Yaquina Head represents a useful location for collecting marine background
air from the Pacific Ocean. The chemical composition of the marine
background air collected at the Yaquina Head site is similar to that for other
remote sites around the world. At Corvallis, "clean" marine background air
can occasionally (21%) be collected even though Corvallis is located 64 km
from the ocean. / Graduation date: 1993
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Factors affecting the saltwater-entry behavior and saltwater preference of juvenile chinook salmon, Oncorhynchus tshawytschaPrice, Carol Seals 09 April 2002 (has links)
From 1998-2000, laboratory studies were conducted to examine factors
that impact saltwater-entry behavior and saltwater preference (SWP) of juvenile
chinook salmon, Oncorhynchus tshawytscha. These factors included bacterial
kidney disease, stress and the presence of trout, O. mykiss. An additional study
investigated the orientation of the startle response of chinook salmon within a
salinity gradient. All experiments were conducted in 757-1 tanks in which a
stable, vertical salinity gradient was established. SWP was decreased in fish
suffering from bacterial kidney disease (31 �� 20.0%), compared with control fish
(85 �� 17.6%). A mild chasing stressor resulted in a 26% decrease in SWP relative
to unstressed fish. After a severe handling stressor, only 20% of fish preferred
salt water, compared with 100% of unstressed controls. After exposure to an
overhead predator model, severely stressed fish descended into the saltwater layer, but this response was transient. The presence of non-aggressive steelhead trout did not affect SWP of chinook salmon. Chinook salmon stocked with rainbow trout displayed decreased SWP. Aggression levels in tanks with
rainbow trout were higher than in tanks with only chinook salmon. The
orientation of the startle response was affected by the presence of salt water.
Fish that preferred salt water within a gradient responded by moving
horizontally within the saltwater layer. In contrast, control fish (held only in
freshwater) moved vertically within the water colunm when startled. Prior
preference for salt water superseded the inclination to move upward in the water
column when startled.
Smoltification involves physiological, behavioral and morphological
changes that prepare healthy chinook salmon for seawater residence. However,
disease, stress and aggressive interactions can decrease the SWP of fish at this life
history stage. Avoidance of salt water during estuarine outmigration is likely
maladaptive, and may have ecological ramifications including increased risk of
avian predation during outmigration and decreased fitness in the marine
environment. / Graduation date: 2002
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Spring phytoplankton dynamics in a shallow, turbid coastal salt marsh system undergoing extreme salinity variation, South TexasHebert, Elizabeth Michele 29 August 2005 (has links)
The contribution of phytoplankton productivity to higher trophic levels in salt marshes is not well understood. My study furthers our understanding of possible mechanisms controlling phytoplankton productivity, abundance, and community composition in salt marshes. Across three consecutive springs (2001 to 2003), I sampled the upper Nueces Delta in south Texas, a shallow, turbid, salt marsh system stressed by low freshwater inflow and wide ranging salinity (<15 to >300 ppt). Water column productivity and respiration were estimated using a light-dark bottle technique, and phytoplankton biovolume and community composition were determined using inverted light microscopy. To determine their effect on the phytoplankton community, zooplankton and bacterioplankton abundance and several physical parameters were also assessed. Meaningful relationships among the numerous variables evaluated in this study were identified using principal component analysis (PCA). Despite high turbidity, phytoplankton productivity and biovolume were substantial. Resuspension appeared to play a major role in phytoplankton dynamics, as indicated by a positive relationship between ash weight and biovolume that explained up to 46% of the variation in the PCA. Negative relationships between zooplankton grazers and pennate diatoms of optimal sizes for these grazers suggested a functional grazing food chain in this system. Salinity also may have been important in phytoplankton dynamics, whereas nutrients appeared to play a minor role. Salinity increases may have been responsible for a decoupling observed between phytoplankton and grazers during late spring. Findings suggest hypotheses for future studies focused on the role of phytoplankton in salt marshes, particularly those stressed by reduced freshwater inflow and high salinities.
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Elucidation of the epithelial sodium channel as a salt taste receptor candidate and search for novel salt taste receptor candidatesRiedel, Katja January 2011 (has links)
Salty taste has evolved to maintain electrolyte homeostasis, serving as a detector for salt containing food. In rodents, salty taste involves at least two transduction mechanisms. One is sensitive to the drug amiloride and specific for Na+, involving epithelial sodium channel (ENaC). A second rodent transduction pathway, which is triggered by various cations, is amiloride insensitive and not almost understood to date. Studies in primates showed amiloride-sensitive as well as amiloride-insensitive gustatory responses to NaCl, implying a role of both salt taste transduction pathways in humans. However, sensory studies in humans point to largely amiloride-insensitive sodium taste perception. An involvement of ENaC in human sodium taste perception was not shown, so far. In this study, ENaC subunit protein and mRNA could be localized to human taste bud cells (TBC). Thus, basolateral αβγ-ENaC ion channels are likely in TBC of circumvallate papillae, possibly mediating basolateral sodium entry. Similarly, basolateral βγ-ENaC might play a role in fungiform TBC. Strikingly, δ-ENaC subunit was confined to taste bud pores of both papillae, likely mediating gustatory sodium entry in TBC, either apical or paracellular via tight junctions. However, regional separation of δ-ENaC and βγ-ENaC in fungiform and circumvallate TBC indicate the presence of unknown interaction partner necessary to assemble into functional ion channels. However, screening of a macaque taste tissue cDNA library did neither reveal polypeptides assembling into a functional cation channel by interaction with δ-ENaC or βγ-ENaC nor ENaC independent salt taste receptor candidates. Thus, ENaC subunits are likely involved in human taste transduction, while exact composition and identity of an amiloride (in)sensitive salt taste receptors remain unclear.
Localization of δ-ENaC in human taste pores strongly suggests a role in human taste transduction. In contrast, δ-ENaC is classified as pseudogene Scnn1d in mouse. However, no experimental detected sequences are annotated, while evidences for parts of Scnn1d derived mRNAs exist. In order to elucidate if Scnn1d is possibly involved in rodent salt taste perception, Scnn1d was evaluated in this study to clarify if Scnn1d is a gene or a transcribed pseudogene in mice. Comparative mapping of human SCNN1D to mouse chromosome 4 revealed complete Scnn1d sequence as well as its pseudogenization by Mus specific endogenous retroviruses. Moreover, tissue specific transcription of unitary Scnn1d pseudogene was found in mouse vallate papillae, kidney and testis and led to identification of nine Scnn1d transcripts. In vitro translation experiments showed that Scnn1d transcripts are coding competent for short polypeptides, possibly present in vivo. However, no sodium channel like function or sodium channel modulating activity was evident for Scnn1d transcripts and/or derived polypeptides. Thus, an involvement of mouse δ-ENaC in sodium taste transduction is unlikely and points to species specific differences in salt taste transduction mechanisms. / Der Salzgeschmack ermöglicht elektrolytreiche Nahrungsquellen zu erkennen und ist eine essentielle Komponente für den Erhalt des Elektrolythaushalts. In Nagern sind bisher zwei Mechanismen bekannt, welche an der Vermittlung des Salzgeschmacks beteiligt sind. Ein Natrium-spezifischer, Amilorid-sensitiver Signaltransduktionsweg wird über den epithelialen Natriumkanal (ENaC) vermittelt. Ein weiterer, bisher ungeklärter Transduktionsweg, ist Amilorid-unempfindlich und wird durch verschiedene Kationen vermittelt. Studien in Primaten konnten Amilorid-sensitive als auch -insensitive gustatorische Signaltransduktionswege nachweisen, wohingegen sensorische Studien auf eine Amilorid-Unempfindlichkeit des Natrium-spezifischen humanen Salzgeschmacks hinweisen. Eine Beteiligung des ENaC bei der Vermittlung des menschlichen Salzgeschmacks wurde bislang nicht gezeigt. In dieser Arbeit konnte die mRNA als auch Proteine von ENaC Untereineiten in menschlichen Geschmacksrezeptorzellen (GRZ) lokalisiert werden. Demzufolge, sind αβγ-ENaC Ionenkanäle möglicherweise an einem basolateralen Natriumeinstrom in circumvallaten GRZ beteiligt. Die basolaterale Lokalisation von βγ-ENaC in fungiformen GRZ weißt auf eine gleichartige Funktion hin. Die außergewöhnliche Lokalisation der δ-ENaC Untereineit ausschließlich in der Porenregion von Geschmacksknospen beider Geschmackspapillen, legt eine Beteiligung dieser ENaC Untereinheit bei der Vermittlung geschmacksrelevanter apikaler bzw. transzellulärer Natriumströme nahe. Gleichwohl weist die räumliche Trennung von apikalen δ-ENaC und basolateralen βγ-ENaC auf die Existenz unbekannter Interaktionspartner hin, da beide getrennt voneinander nicht in der Lage sind effektive Natriumkanäle zu assemblieren. Die Durchmusterung einer geschmacksrelevanten cDNA Bibliothek führte weder zur Identifikation von ENaC Interaktionspartnern, noch von ENaC unabhängigen Polypeptiden, welche in der Lage sind einen Kationenkanal zu bilden. Die genaue Zusammensetzung humaner Amilorid- (in)sensitiver Salzrezeptoren bleibt daher unklar und ein spannendes Feld.
Der Nachweis von ENaC in humanen GRZ und insbesondere die Poren assoziierte Lokalisation der δ-ENaC Untereinheit impliziert eine wichtige Rolle bei der gustatorischen Signaltransduktion. Erstaunlicherweise ist die orthologe δ-ENaC Untereinheit der Maus als Scnn1d Pseudogen klassifiziert. Neben dieser automatischen Annotierung sind keine experimentell ermittelten Sequenzen in Datenbanken hinterlegt obwohl Scnn1d abgeleitete mRNA nachgewiesen werden konnte. Im Rahmen dieser Arbeit wurde untersucht ob Scnn1d ein Gen oder ein transkribiertes Pseudogen ist, um eine mögliche Rolle bei der Transduktion des murinen Salzgeschmacks zu klären. Durch Sequenzabgleich mit humanen SCNN1D konnte das vollständige Scnn1d Gen auf dem Chromosom 4 der Maus identifiziert werden, wobei sich dessen Pseudogenisierung durch Mus spezifische endogene Retroviren zeigte. Darüber hinaus wurden neun gewebsspezifische Scnn1d Transkripte nachgewiesen, welche für kurze Polypeptide kodieren. Eine mögliche Funktion derselben als Ionenkanal bzw. eine modulatorische Funktion konnte nicht gezeigt werden. Eine Beteiligung des pseudogenisierten δ-ENaC an der Vermittlung des Salzgeschmacks der Maus ist daher unwahrscheinlich und deutet auf Speziesunterschiede der Salzgeschmacksvermittlung hin.
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The Effect of Mechanical Characteristics of Basal Decollement and Basement Structures on Deformation of the Zagros BasinBahroudi, Abbas January 2003 (has links)
Two fundamental structural elements, basement architecture and basal decollement, play a significant role in the evolution of a tectonically active region. Using different approaches (field data, literature review and analogue models) this thesis demonstrates that these two elements affected the deformation style in the Zagros fold-thrust belt during Mesozoic extensional and Cenozoic contractional episodes. Reassessment of available data suggests a new configuration for the basement to Zagros basin that consists of basement faults with three different trends. Complicated interrelationships of these basement faults divide the Zagros basin into two major basement blocks, active and passive. This model associated with geological evidence suggests that deformation in the basement due to the convergence between Arabia and Iran is not restricted to the Zagros Mountains but already involves a considerable part of the Arabian platform. However, deformation in the cover units is not only governed by the deformation in the basement, which are decoupled from each other by the Hormuz salt in many parts in the Zagros fold-thrust belt. Geological evidence shows that there is a clear relationship between activity of the Hormuz structures and the basement faults in the Zagros basin. Extended analogue models indirectly show how Hormuz types of basal decollement associated with the opening of Neo-Tethys can control the distribution, number, width and geometry of faults, penetrative strain, and diapirism. Analogue models shortened from one-end show that the spatial distribution of the Hormuz salt in the Zagros belt map viscous and frictional decollements to the thin-skinned deformation. Shortening of the cover results in formation of partitioning of strain into transpressional zones, different topographic wedges and differential sedimentation of growth sediments along the Zagros belt. Model results supported by geological and geophysical data sets suggest that some of the faults previously attributed to basement could have developed above the initial lateral boundaries between viscous and frictional basal decollements.
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