Abstract Stickies refer to tacky contaminants in recovered paper, excluding wood extractives. Stickies originate from adhesives, ink binders and coating binders. Stickies able to pass a sieve of 100 or 150 μm (depending on standard) are called micro stickies, whereas the particles retained on the screen are called macro stickies. Dissolved and colloidal stickies are called secondary stickies. There are standard methods for macro stickies, but a standard method measuring the total amount of stickies is lacking. Furthermore, the size distribution, and nature of stickies in the sense if they are free particles or agglomerated with some other substances, has not been previously known. The information on the removal of stickies in different unit operations was also not known very well except for macro stickies. The aim of this thesis was thus to develop an analysis method for the total amount of stickies, determine the size distribution and nature of stickies, and find ways to better remove stickies in the deinking process. Extraction with tetrahydrofuran (THF) and High Pressure Liquid Chromatography (HPLC) with Size Exclusion Column (SEC) was used here to separate hydrophobic polymers (stickies) from the pulp suspension. An Evaporating solvent Light Scattering (ELS) detector was utilized to quantify the polymers after SEC. This analysis procedure was used in this thesis to measure stickies. Fractionation of pulp before the analysis was carried out if information of different-sized stickies was desired. The majority of stickies are micro stickies. A significant, although lower, portion of stickies are macrostickies, especially in the beginning of the deinking process. The dissolved and colloidal phase was found to contain wood extractives, and only trace amounts of stickies. Flotation is very effective in stickies removal. Ink and micro stickies were removed nearly equally in flotation, because they both are hydrophobic and are both in an optimal size range for removal in flotation. Ink measurements may be used for estimating the trends of stickies removal in flotation. The stickies removal in flotation may be enhanced by optimizing the pulper chemistry.

The membrane system of a eukaryotic cell is a large and complex system handling the transport, exchange and degradation of many kinds of material. Recent research shows that double-stranded RNA (dsRNA) mediated gene silencing (RNA interference) is a membrane related process. After long dsRNA is processed to small interfering RNA (siRNA) by Dicer, the guide strand and passenger strand are separated in the RNA induced silencing complex (RISC) by Argonaute. The process of loading siRNA into RISC has been suggested to occur at the rough Endoplasmic Reticulum (rER).The components of RISC also associate with late endosomes/multivesicular bodies (MVBs). Furthermore, disturbing the balance between late endosomes/MVBs and lysosomes has been shown to affect the efficiency of silencing. We use the nematode Caenorhabditis elegans as our model organism to study two questions: how does membrane transport affect RNAi and spreading of RNAi from the recipient cells to other tissues (systemic RNAi); and how does RNA transport contribute to the multigenerational silencing induced by dsRNA (RNAi inheritance)? Using SID-5, a protein required for efficient systemic RNAi, as bait in a yeast two-hybrid (Y2H) screen, we got 32 SID-5 interacting candidate proteins. Two of these are the SNARE protein SEC-22 and the putative RNA binding protein C12D8.1. In two additional Y2H screens, we found that SID-5 interacts with multiple syntaxin SNAREs, including SYX-6, whereas SEC-22 only interacts with SYX-6. SNAREs usually function in vesicle fusion processes. We found the two SNARE proteins SEC-22 and SYX-6 to be negative regulators of RNAi and to localize to late endosomes/MVBs. In addition, loss of sid-5 leads to an endosome maturation defect. Finally, we found that the putative RNA binding protein C12D8.1 negatively regulates RNAi inheritance and that C12D8.1 mutant animals show impaired RNAi upon targeting a new gene. Taken together, the results presented in this thesis provide us with more evidence for the connection of the membrane transport system and RNAi. The identification of a putative negative regulator of RNAi inheritance further enriches this research field.

Chez la bactérie Escherichia coli, SecB appartient au réseau de chaperons moléculaires qui assistent le repliement et l'adressage des protéines nouvellement synthétisées. SecB est connu pour faciliter l'export en interagissant avec les pré-protéines sous forme non-native et les adressant au translocon Sec via une interaction directe avec le moteur ATPase SecA. SecB possède aussi une activité de chaperon générique et est capable d'assister le repliement de certaines protéines cytosoliques en absence des chaperons majeurs DnaK et Trigger factor. Alors que le translocon Sec est universellement conservé, le chaperon SecB est retrouvé principalement chez les protéobactéries. Cependant, de plus en plus de séquences de type SecB sont retrouvées dans d'autres groupes de la taxonomie bactérienne, comme par exemple chez le pathogène humain majeur Mycobacterium tuberculosis. Chez cette bactérie, une séquence de type SecB appelée Rv1957 est présente en association avec un système toxine-antitoxin (TA) appartenant à la famille HigBA. Généralement, les TA sont des systèmes à deux composants qui modulent la croissance en réponse à des conditions de stress spécifiques, favorisant ainsi l'adaptation et la persistance. Dans le cas de ce système atypique toxine-antitoxine-chaperon (TAC), Rv1957 interagit avec l'antitoxine HigA et la protège à la fois de l'agrégation et de la dégradation, et est donc strictement requis pour permettre l'inactivation de la toxine par l'antitoxine. La première partie de ce travail avait pour but de reconstruire l'histoire évolutive de ce nouveau système TAC. Pour cela nous avons procédé à une recherche de systèmes similaires dans l'ensemble des génomes disponibles qui a révélé que la présence de systèmes TAC n'est pas limitée aux mycobactéries et que ces systèmes semblent s'être répandus dans la taxonomie par le biais de transferts horizontaux de gènes. Nos résultats suggèrent que les chaperons des systèmes TAC sont évolutivement apparentés au chaperon d'export solitaire SecB et ont divergé pour devenir spécialisés vis-à-vis de leurs antitoxines partenaires. Nous avons ensuite étudié ce phénomène de spécialisation par une approche d'évolution dirigée du chaperon d'export SecB d'E. coli. Nous avons mis en évidence que des substitutions uniques dans SecB sont suffisantes pour améliorer sa capacité à contrôler spécifiquement HigBA du système TAC, et que ces mutations résultaient généralement en une meilleure interaction avec l'antitoxine HigA. Remarquablement, environ la moitié des mutants identifiés sont affectés dans leur activité de chaperon générique en l'absence des chaperons DnaK et Trigger factor, suggérant un conflit entre spécialisation du chaperon et ses fonctions génériques. La plupart des résidus identifiés se trouvent dans une région de SecB non caractérisée et proche du site proposé d'interaction avec le substrat. Des expériences de cross-link à des positions spécifiques ont révélé que cette région interagit directement avec l'antitoxine HigA. Enfin, nous avons montré que HigA est capable d'entrer en compétition avec la fonction d'export d'un SecB spécialisé plus efficacement que pour la version sauvage de SecB, illustrant la potentielle connexion entre les fonctions de type SecB dans l'export et le contrôle d'un système TA. / SecB is part of the intricate network of chaperones that assist folding and targeting of newly synthesized polypeptides in Escherichia coli. SecB is known to interact with nonnative precursor proteins and address them to the Sec translocon via direct interaction with the SecA motor component, thus facilitating their export. SecB is also able to act as a generic chaperone, by assisting the folding of certain cytosolic proteins when the major DnaK/Trigger Factor chaperone pathway is disrupted. While the Sec translocon is universally conserved, the SecB chaperone is mainly found in proteobacteria. However, an increasing number of SecB-like sequences have been found in unusual groups of bacteria and especially in the major human pathogen Mycobacterium tuberculosis. In this bacterium, a SecB-like sequence, Rv1957, is present in association with a toxin-antitoxin (TA) system belonging to the HigBA family. Usually, TA modules are two-component systems that modulate growth in response to specific stress conditions, thus promoting adaptation and persistence. In the case of this atypical toxin-antitoxin-chaperone (TAC) system, Rv1957 interacts with the HigA antitoxin and protects it from both aggregation and degradation, and is thus strictly required for neutralization of the toxin by the antitoxin. The first aim of this work was to reconstruct the evolutionary history of the newly discovered TAC system. We performed a large-scale genome screening and found that TAC is not restricted to mycobacteria and seems to have disseminated in the taxonomy by horizontal gene transfer. Our results suggest that TAC chaperones are evolutionarily related to the solitary export chaperone SecB and have diverged to become specialized towards their cognate antitoxins. Next, we investigated such chaperone specialization event through directed evolution of the E. coli export chaperone SecB. We found that single amino-acid substitutions within SecB were sufficient to improve its ability to specifically control HigBA from TAC, and that these mutations mainly resulted in an increased binding to the HigA antitoxin. Strikingly, about half of the mutants identified were affected in their ability to perform SecB generic chaperone functions in the absence of both DnaK and TF chaperones, suggesting a conflict between specialization and generic chaperone functions. Most of the residues identified are located within a previously uncharacterized region of SecB which is close to the proposed substrate binding site. Further in vitro site-specific cross-linking experiments revealed that this region directly interacts with the HigA antitoxin. Finally, we show that the HigA antitoxin can compete with the export function of specialized SecB more efficiently than it does with wild type SecB, thus illustrating the potential interplay between SecB-like chaperone export functions and TA activation.

The chondroitin sulphate proteoglycan (CSPG) versican forms complexes with hyaluronan (HA), which are essential in a range of functions including cellular proliferation and migration. Four isoforms of versican result from alternative splicing. Furthermore, biological roles have been identified for the proteolytic cleavage product of versican which contains the N-terminal G1 hyaluronan binding domain. All of these versican forms have different tightly regulated tissue expression profiles. Consequently, impaired regulation is associated with a number of disease pathologies. For example the largest variants (V0/V1) have been shown to be negative indicators of disease outcome in a number of malignant cancers and are a marker of disease progression in atherosclerosis. Interestingly, the smaller versican isoform V3 which lacks CS chains has been demonstrated to have the potential to reverse disease associated phenotypes. The motivation for carrying out the work in this thesis was to try and gain a better understanding of how versican functions on a molecular scale. In this regard, the first aim was to investigate the structure of the hyaluronan binding region of versican using a construct called VG1. The structure of VG1 was analysed in the presence and absence of hyaluronan oligomers. This revealed an insight into the multi-modular structure of the versican hyaluronan binding region and demonstrated that on binding to HA, VG1 under goes a conformational change. Furthermore, the interaction between VG1 and longer lengths of hyaluronan (pHA) was investigated. This demonstrated that when VG1 binds to pHA it is does so with positive cooperativity, packing very close to neighbouring VG1 molecules along a chain of HA. One consequence of this interaction was to reorganise pHA into a helical conformation, an organisation that was confirmed by a number of solution phase techniques. The effect of this reorganisation of pHA by VG1 on HA/CD44 interactions was also assessed. Previously the interaction between CD44 (a cell surface hyaluronan receptor) and long chains of HA (>30 kDa) was shown to be irreversible; however we demonstrate that VG1 can reverse this. Furthermore, a TSG-6 enhanced CD44/interaction was also completely reversed by the addition of VG1. This provides an indication that a functional hierarchy of hyaluronan binding proteins may exist which could have important implications in understanding the function of hyaluronan complexes. Currently, we do not know whether intact versican molecules could interact with HA in the same way as VG1. However, preliminary data suggests that the CS-containing variants (i.e. V0, V1 and V2) would not, whereas V3 and versican fragments could. This work provides an exciting mechanistic insight into the function of versican variants and their breakdown products.

L’étude concerne un nouveau procédé de reformage du gaz naturel en gaz de synthèse par le dioxyde de carbone (RSM), en vue de l'utilisation rationnelle des déchets carbonés industriels pour la production d'hydrocarbures et d'hydrogène. Cette méthode utilise des systèmes à membranes catalytiques inorganiques (SMC) qui favorisent des réactions catalytiques hétérogènes en phase gazeuse dans des micro-canaux céramiques. La surface active des catalyseurs formés à l'intérieur des canaux est faible en termes de superficie, mais elle est caractérisée par une valeur élevée du facteur Surface/Volume du catalyseur, qui induit une efficacité importante de la catalyse hétérogène. Les SMC, formés à partir de dérivés alcoxy et des précurseurs métalliques complexes, contiennent de 0,008 à 0,055% en masse de nano-composants mono- et bimétalliques actifs répartis uniformément dans les canaux. Pour les systèmes [La-Ce]-MgO-Ti02/Ni-Al et Pd-Mn-Ti02/Ni-Al, les productivités de 10500 et 7500 1/h·dm3membr. ont été respectivement obtenues lors du RSM dès 450°C avec une composition de gaz de synthèse H2/?? allant de 0,63 à 1,25 et un taux de conversion de 50% de la charge CH4/CO2 (1/1). Ainsi les SMC sont d’un ordre de grandeur plus efficace qu’un réacteur à lit fixe du même catalyseur. Le RSM est initié par l'oxydation de CH4 par l'oxygène de structure des oxydes métalliques présents en surface, et le CO2 réagit avec le carbone finement divisé provenant de la dissociation de CH4. Une synergie catalytique a été mise en évidence pour le système Pd-Mn. Ces SMC de 108 pores par cm² de surface constituent un ensemble de nano réacteurs de fort potentiel industriel (synthèse d’oléfines, biomasse) / This study reports the development of a new process to convert methane and carbon dioxide (dry methane reforming - DMR) into valuable products such as syngas from non-oil resources. The practical interest is to produce syngas from carbon containing exhaust industrial gases. This process uses membrane catalytic systems (MCS) that support heterogeneous catalytic reactions in gaseous phase in ceramic micro-channels. The active surface of the catalysts formed inside the micro-channels is low in term of area, but it is characterized by a high value of the catalyst surface/volume ratio, which induces a high efficiency of heterogeneous catalysis. The SMC are formed from alkoxy derivatives and precursor metal complex containing between 0.008 and 0.055% by weight of nano-components mono-and bimetallic active distributed evenly in the channels. For systems [La-Ce] -MgO-Ti02/Ni-Al and Pd-Mn-Ti02/Ni-Al, productivities of 10500 and 7500 l/h · dm3 membr. were respectively obtained by RSM at 450°C with a composition of syngas H2/?? ranging from 0.63 to 1.25 and a conversion rate of 50% with a CH4/CO2 (1/1) feed. Thus the CMS is an order of magnitude more efficient than a fixed bed reactor of the same catalyst. The MDR is initiated by the oxidation of CH4 by structural oxygen of metal oxides available on the surface, and the CO2 reacts with the finely divided carbon arising from the dissociation of CH4. A catalytic synergy has been demonstrated for the system Pd-Mn. This CMS, having 108 pores per cm² of surface, can be considered as a set of nano reactors. Thus this new approach is very promising for industry (synthesis of olefins, uses of biomass)

Orientador: Guimarães, Eduardo Roberto Junqueira Guimarães / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Estudos da Linguagem / Made available in DSpace on 2018-08-04T02:17:53Z (GMT). No. of bitstreams: 1 Nascimento_AnaClaudia_M.pdf: 171332 bytes, checksum: 255cbdc0fe8b3be485a08e74de76e213 (MD5) Previous issue date: 2004 / Resumo: O que é referir e o que é designar? Filiando-nos ao posicionamento teórico da Semântica do Acontecimento, procuramos caracterizar a distinção entre a noção de referência e a noção de designação. Nesse sentido, designar na linguagem não tem a ver meramente com a identificação de um objeto no mundo. A significação de uma expressão referencial é constituída no funcionamento da língua, no confronto de dizeres e sentidos diversos e é esse espaço de constituição de sentido que produz a designação. Para Guimarães (2002), esses espaços são configurados por acontecimentos enunciativos e é aí que se dão as cenas enunciativas, que são modos específicos de acesso à palavra. Analisamos, assim, como se constituem os modos de referir o presidente Lula na época da eleição e, por assim dizer, como se constituem as expressões que designam esse sujeito. Os modos de referir recortados constituem a designação do sujeito em questão e, na medida em que as designações mobilizam dizeres e sentidos outros, identificam Lula nos acontecimentos estudados, particularizando-o. Tomamos, para isso, seqüências de textos extraídos de Veja, Época e Isto É Gente e procuramos mostrar como o espaço enunciativo que faz funcionar esses periódicos é um espaço de política do dizer, em que o dizer se distribui desigualmente. A política do dizer constitui os espaços enunciativos, os dizeres que aí se produzem e, por assim dizer, o modo como Lula é identificado em cada uma das revistas / Abstract: What does refer mean and what does designation mean? We are on the theoretical position from Semântica do Acontecimento, and we try to establish a distinction between the conception of reference and the conception of designation. Thus, refer in the language is not related to identify an object in the world. The sense of the referent in the language is constituted on language working, where there is a confrontation of several sayings and several senses, and this is the space where the designation is produced. According Guimarães (2002), the spaces of constitution of the sense are configured by acontecimentos enunciativos and the enunciative scenes happen there ¿ enunciative scenes are ways of specific access to speaking. We analyze how the ways of referring the president Lula in election time and, then, how the designative expressions of this person are constituted. The cutting ways of referring designate Lula and, more they make ready other sayings and other senses, more they identify Lula in those enunciations. For that, we took sequences of texts from Veja, Época e Isto É Gente and we tried to show how the enunciative space, where those magazines work, is a political space, where the speaking is not distributed equally. The politic question constitutes the enunciative spaces, the enunciation and, then what identifies Lula in each magazine / Mestrado / Linguistica / Mestre em Linguística

Orientador: Maria Carolina Boverio Galzerani / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Educação / Made available in DSpace on 2018-08-03T15:41:45Z (GMT). No. of bitstreams: 1 Bueno_JoaoBatistaGoncalves_M.pdf: 9595364 bytes, checksum: 68b1aae7a4e24e48608a5abfc13fa582 (MD5) Previous issue date: 2003 / Resumo: Faço uma primeira aproximação com a história do livro didático, buscando dar visibilidade e explicitar com maior clareza o uso de imagens em livros didáticos de história. Neste caso, procuro desvendar para o leitor, formas e práticas de leitura iconográficas que ocorreram no final do século XIX comparando-as com as práticas de leitura de imagem do final do século XX. É importante destacar que trabalho as metodologias utilizadas para realizar as análises iconograficas. Bem como, traço um panorama que procura desvendar o estado das pesquisas que focalizam os temas sobre livros didáticos e sobre as ilustrações nos livros no Brasil. A partir do estudo teórico parto para as análises de livros didáticos de história, um do final do século XIX, um do início do século XX e um do final do século XX. Faço portanto, uma análise das diferentes práticas de leitura iconográfica indicadas por esses documentos. Finalmente faço considerações, que buscam propor a professores e alunos, formas de leitura de imagens como documento histórico e que podem ser aplicadas no meio escolar / Abstract: I make a flfst approach with the history of the didactic book, searching to give visibility and to explain with bigger clarity the use of images in didactic books of history. In this in case that, I look to unrnask for the reader, iconographic practical fonns and reading that had occurred in the end of century XIX comparing them with the practical ones reading image of the end of century xx. It is important to detach that work the used methodologies to carry through the iconographic analyses. As well as, trace a panorama that it looks to unmask the state of the research that focuses the subjects on didactic books and illustrations in books in Brazil. From the theoretical study history I go on to the didactic book analyses of history, one of the end of century XIX, one of the beginning of century XX and one of the end of century xx. I make therefore, an analysis of different the practical ones of iconographic reading indicated by these documents. Finally I make consideration, that they search to consider the professors and student, forros of reading of images as historical document and that they can be applied in the halfpertaining to school / Mestrado / Educação, Conhecimento, Linguagem e Arte / Mestre em Educação

Orientadores: Helena Jank, Eduardo Ostergren / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Artes / Made available in DSpace on 2018-08-03T16:07:28Z (GMT). No. of bitstreams: 1 Dias_VivianLisFerreiraNogueira_M.pdf: 9752860 bytes, checksum: fdcbcb0800d20af82d0b0c3d1f0320aa (MD5) Previous issue date: 2002 / Resumo: Esta dissertação tem como objetivo a análise de dois motetes do compositor barroco alemão Johann Ludwig Bach (1677-1731), primo em terceiro grau do mestre Johann Sebastian Bach (1685-1750). Dividido em quatro capítulos, o primeiro apresenta um levantamento de dados biográficos do compositor, envolvendo a contextualização histórico-geográfica, grau de parentesco e relacionamento com Johann Sebastian Bach. O segundo capítulo focaliza o desenvolvimento histórico do motete, desde os seus primórdios até o período barroco. Um estudo detalhado dos motetes "Unsere Trübsal" e "Das ist meine Freude", compreendendo a análise das estruturas formal, harmônica e melódica é o conteúdo do terceiro capítulo. As conclusões obtidas na análise são determinantes para que se estabeleçam os parâmetros interpretativos de cada composição e, ao mesmo tempo, fornecem resultados que confirmam a existência de fortes conexões entre texto e música, evidenciadas pela identificação de diversas figuras retórico-musicais, de acordo com a classificação proposta por George Büelow e Dietrich Bartel. A última parte da dissertação propõe ao regente coral, parâmetros para a interpretação destas obras, tendo como determinante principal a estreita relação entre elementos musicais e retóricos, em consonância com o ideal musical do barroco alemão de valorização do texto através da música, confirmado pelas referências de diversos tratados de época / Abstract: The study and analysis of two motets by the German baroque composer Johann Ludwig Bach (1677-1731) is the purpose of this dissertation. Divided in four chapters, the first examines the composer' s biography within an historical-geographic context as well as family relationship to his cousin by third degree Johann Sebastian Bach. The second examines the development of the motet from the earliest beginnings to its more mature form in the baroque period. A detailed study of motets "Unsere Tübsal" and "Das ist meine Freude" ftom the standpoint of formal, harmonic and melodic analysis, provides elements that help determine interpretive parameters for the performance of each composition. Important aspects of music and text relationship have also been observed as well as identification of musical-rhetorical figures in conformity with George Buelow' s and Dietrich Bartel's classification. The last chapter examines the relationship between the musical and rhetorical elements of sacred vocal music of the German baroque whose ideals were to strongly emphasize text through music. Historical treatises are also referred to in order to support these observations / Mestrado / Mestre em Artes

Orientador : Cesar Apareciddo Nunes / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Educação / Made available in DSpace on 2018-07-31T18:09:13Z (GMT). No. of bitstreams: 1 Bastos_PedroIvoRibeirodeAssis_M.pdf: 723917 bytes, checksum: 02d5f465f43c6a1ad90e3dee4f295a0b (MD5) Previous issue date: 2001 / Mestrado

Orientador: Paulo José dos Reis Pereira / Banca: Reginaldo Mattar Nasser / Banca: Rafael Antonio Duarte Villa / O Programa de Pós-Graduação em Relações Internacionais é instituído em parceria com a Unesp/Unicamp/PUC-SP, em projeto subsidiado pela CAPES, intitulado "Programa San Tiago Dantas" / Resumo: O tráfico de drogas é um tema novo na disciplina das Relações Internacionais e vem ganhando destaque na agenda de segurança internacional desde a década de 1990. O fim da Guerra Fria e as decorrentes transformações políticas, econômicas e tecnológicas influenciaram no crescimento da criminalidade organizada transnacional, assim como na mudança de percepção que se tinha sobre ela. Neste cenário, o tema das drogas passou a ser tratado politicamente como uma ameaça ao sistema internacional, aos estados nacionais e aos indivíduos. A noção de "guerra às drogas" foi incorporada pelo regime de proibição global das drogas das Nações Unidas e das demais organizações multilaterais. Ela também pautou a presença norte-americana sobre a América Latina a partir deste período. A postura do Brasil com relação ao tema das drogas mudou significantemente nos anos 1990, quando uma série de aparatos legislativos e institucionais foram estabelecidos com o objetivo de combater o tráfico de drogas, a partir da identificação das ameaças associadas a este crime. A inserção internacional do Brasil neste período foi marcada pelo reconhecimento de questões de segurança que vinham sendo definidas internacionalmente por meio do regime de proibição global das drogas consolidado pelos organismos multilaterais, assim como pela "guerra às drogas" empreendida pelos Estados Unidos sobre a América Latina. O objetivo deste trabalho é analisar se e como o Brasil incorporou as ideias e políticas estabelecidas internacionalmente ao longo da década de 1990, identificando atores estatais e suas decisões, assim como avaliando o alinhamento das concepções e práticas políticas adotadas domesticamente às diretrizes internacionais com base no modelo institucional e legislativo adotado neste período. / Abstract: Drug trafficking is a new subject in the discipline of International Relations and it has gained substantial highlight in the international security agenda since the 1990s. The end of the Cold War and the resulting political, economic and technological changes influenced on the growth of transnational organized crime and on the change of perception upon it. In this scenario, the issue of drugs is treated politically as a threat to the international system, national states and individuals. The notion of "war on drugs" was not only incorporated in the global prohibition regime on drugs, but has also guided the US presence on Latin America during the 1990s. The Brazilian attitude toward the drug problem has changed significantly in the 1990s, when a series of legislative and institutional apparatuses were developed in order to combat drug trafficking, based on the identification of threats associated with that crime. The Brazilian international insertion strategy during the 1990s was followed by the recognition of the security issues internationally defined by the global prohibition regime of on drugs, consolidated by multilateral organizations, as well as the "drug war" waged by the United States on America Latina. This research objective is to identify whether and how Brazil has incorporated the internationally established ideas and policies on drugs throughout the 1990s, identifying political actors and their decisions, as well as evaluating the alignment of concepts and political practices domestically adopted in relations to the international guidelines, based on the institutional and legislative model adopted during the 1990s. / Mestre