x, 72 p. A print copy of this thesis is available through the UO Libraries. Search the library catalog for the location and call number. / The theory of equivariant homology and cohomology was first created by Bredon in his 1967 paper and has since been developed and generalized by May, Lewis, Costenoble, and a host of others. However, there has been a notable lack of computations done. In this paper, a version of the Serre spectral sequence of a fibration is developed for RO ( G )-graded equivariant cohomology of G -spaces for finite groups G . This spectral sequence is then used to compute cohomology of projective bundles and certain loop spaces. In addition, the cohomology of Rep( G )-complexes, with appropriate coefficients, is shown to always be free. As an application, the cohomology of real projective spaces and some Grassmann manifolds are computed, with an eye towards developing a theory of equivariant characteristic classes. / Adviser: Daniel Dugger

>Magister Scientiae - MSc / The study area for this thesis focuses on the central northern part of the Bredasdorp Basin of southern offshore South Africa, where the depositional environments of wells E-M1, E-M3 and E-AB1 were inferred through electro sequence analysis and sequence stratigraphy analysis of the corresponding seismic line (E82-005). For that, the Petroleum Agency of South Africa (PASA) allowed access to the digital data which were loaded onto softwares such as PETREL and Kingdom SMT for interpretational purposes. The lithologies and sedimentary environments were inferred based on the shape of the gamma ray logs and reported core descriptions. The sequence stratigraphy of the basin comprises three main tectonic phases: Synrift phase, Transitional phase and Drift phase. Syn-rift phase, which began in the Middle Jurassic during a period of regional tectonism, consists of interbedded red claystones and discrete pebbly sandstone beds deposited in a non-marine setting. The syn-rift 1 succession is truncated by the regional Horizon ‘C’ (1At1 unconformity). The transitional phase was influenced by tectonic events, eustatic sea-level changes and thermal subsidence and characterized by repeated episodes of progradation and aggradation between 121Ma to 103Ma, from the top of the Horizon ‘C’ (1At1 unconformity) to the base of the 14At1 unconformity. Finally the drift phase was driven by thermal subsidence and marked by the Middle Albian14At1 unconformity which is associated with deep water submarine fan sandstones. During the Turonian (15At1 unconformity), highstand led to the deposition of thin organic-rich shales. In the thesis, it is concluded that the depositional environment is shallow marine, ranging from prograding marine shelf, a transgressive marine shelf and a prograding shelf edge delta environment.

Submitted to the faculty of the School of Informatics in partial fulfillment of the requirements for the degree Master of Science in Bioinformatics in the School of Informatics,Indiana University July, 2004 / DNA markers have revolutionized the field of genetics by increasing the pace of genetic analysis. Simple sequence repeats (SSRs) are repetitions of nucleotide motifs of 1 to 5 bases and are currently the markers of choice in many plant and animal genomes due to their abundant distribution in the genomes, hypervariable nature and suitability for high-throughput analysis. While SSRs, once developed, are extremely valuable, their development is time consuming, laborious and expensive. Sequences from many genomes are continuously made freely available in the public databases and mining of these sources using computational approaches permits rapid and economical marker development. Expressed sequence tags (ESTs) are ideal candidates for mining SSRs not only because of their availability in large numbers but also due to the fact that they represent expressed genes. Large scale SSR mining efforts in plants to date focused on monocotyledonous plants. In this project, an efficient SSR identification tool was developed and used to mine SSRs from more than 53 dicotyledonous species. A total of 92,648 non-redundant ESTs or 6.0% of the 1.54 million dicotyledonous ESTs investigated in this study were found to contain SSRs. The frequency of non-redundant-ESTs containing SSRs among the species investigated ranged from 2.65% to 16.82%. More than 80% of the non-redundant ESTs having SSRs contained a single SSR repeat while others contained 2 or more SSRs. An extensive analysis of the occurrence and frequencies of various SSR types revealed that the A/T mononucleotide, AG/GA/CT/TC dinucleotide, AAG/AGA/GAA/CTT/TTC/TCT trinucleotide and TTTA and TTAA tetranucleotide repeats are the most abundant in dicotyledonous species. In addition, an analysis of the number of repeats across species revealed that majority of the mononucleotide SSRs contained 15-25 repeats while majority of the di- and tri-nucleotide SSRs contained 5-10 repeats. By providing valuable information on the abundance of SSRs in ESTs of a large number of dicotyledonous species, this study demonstrates the potential of computational mining approach for rapid discovery of SSRs towards the development of markers for genetic analysis and related applications.

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Volvo Group’s cab plant in Umeå produces cab bodies and consists of three operating areas; the Stamping and parts production, the Body in White and the Paint shop. Today the plant produces around XXX cabs/week, but the goal is to reach the invested capacity of XXX cabs/week. In order to increase the production capacity, the efficiency of both the manual and the automated processes need to be improved. Unlike the manual processes in where the capacity can be increased by enlarged workforce, the automated processes need to be optimized. Today the cabs are produced in the same order as the orders are received. The cab plant wants to investigate if the capacity of the automated segment in the Body in White unit can be increased by changing the order in the production sequence. This culminates in the following problem definition:  Is there untapped potential of the Body in White that can be achieved by an optimization of the production sequence? If so, how can Volvo Group’s cab plant in Umeå utilize this knowledge in the production planning process? The objective of the project was achieved by combining discrete event simulation with a heuristic optimization approach. The results shows that long batches in the production sequence limit the throughput of the system. By spreading the unfavorable subsequences of batches evenly over the production sequence, the total throughput of the Body in White can be increased by 52 cabs/week. / Volvokoncernens hyttfabrik i Umeå producerar förarhytter och är uppdelad i tre driftsområden; pressning och detaljtillverkning, hyttsammansättning och ytbehandling. Idag producerar fabriken ca XXX hytter/vecka, men möjlighet finns att nå upp till den investerade kapaciteten på XXX hytter/vecka. För att kunna öka produktionskapaciteten behöver både de manuella och de automatiserade processerna effektiviseras. Till skillnad från de manuella processerna, där kapaciteten kan ökas genom att öka antalet operatörer, behöver de automatiserade processerna optimeras. Idag produceras hytterna i samma ordning som beställningarna kommer in. Hyttfabriken vill undersöka om det finns en möjlighet att öka kapaciteten för den automatiserade processen i Body in White genom att optimera produktionssekvensen. Detta leder fram till följande problemformulering: Finns det outnyttjad potential i Body in White som kan uppnås genom att optimera produktionssekvensen? Hur kan Volvokoncernens hyttfabrik i Umeå utnyttja denna kunskap vid planering av produktionen? Målet med projektet har uppnåtts genom att kombinera diskret händelsesimulering med optimering genom heuristiker. Resultatet visar att stora batcher av samma variant i produktionen begränsar genomströmningen i processen. Genom att bland annat sprida ut de ogynnsamma delsekvenserna jämnt över produktionssekvensen kan den totala genomströmningen i Body in White ökas med 52 hytter/vecka.

ODC activity of the altered proteins was measured and compared to that of the full length 461 amino acid containing ODC. Mouse ODC cDNA sequences were deleted from either 5' or 3' ends using exonuclease III and Mung Bean nuclease treatments. An internal deletion was obtained by Hinc II and Bcl I restriction endonuclease digestion of the full length ODC cDNA. Capped mRNAs were synthesized in vitro using the resulting deleted DNA as templates, and the protein was translated in vitro. The results indicate that the protein in which translation initiates at internal AUG start codons does not have any activity. The protein with 39 amino acids deleted from carboxy-terminus maintains 12% of the activity, while deletion of greater than 79 amino acids have no activity. An internal deletion from amino acid 290 to 331 and which may contain the suspected ornithine binding site has no activity. These results suggest that the entire amino acid sequence of mouse ODC is required for full activity of the enzyme.

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